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The map label for this gene is ispG [H]

Identifier: 126452211

GI number: 126452211

Start: 2216794

End: 2218044

Strand: Reverse

Name: ispG [H]

Synonym: BURPS1106A_2228

Alternate gene names: 126452211

Gene position: 2218044-2216794 (Counterclockwise)

Preceding gene: 126454923

Following gene: 126453409

Centisome position: 55.61

GC content: 69.46

Gene sequence:

>1251_bases
GTGTTCGGCGGGCATGCGCCGCGGCGCGTGTCGCATGCGGTCGATGTCCGCTGGGGCGGCACGCTCGTGACGATCGGCGG
CGCGGCGCCCGTGCGCGTGCAGTCGATGACGAACACCGATACGGCCGACGCGATCGGCACCGCGATCCAGGTGAAGGAGC
TCGCGAACGCGGGCTCCGAGCTCGTGCGCATCACCGTGAACACGCCGGAGGCGGCCGCTGCCGTGCCGGCGATTCGCGAG
CAGCTCGACCGGATGGGCGTGACGGTGCCGCTTGTCGGCGATTTCCACTACAACGGCCACCTGCTGCTGCGCGACTACCC
GGACTGCGCGCAGGCGCTGTCGAAATACCGGATCAACCCGGGCAACGTCGGCCAGGGCGCGAAGCGCGATTCGCAGTTCG
CGCAGATGATCGAAGCCGCGATCAAGTACGACAAGCCGGTGCGGATCGGCGTGAACTGGGGCAGCCTCGATCAGGACCTG
CTCGCGCGGATGATGGACGAGAACGGCGCGCGCGCCGAGCCGTGGGAGGCGCAGAGCGTGATGTACGAGGCGCTGATCCA
GTCGGCGATCGGCTCGGCCGAGCGCGCGGTCGAGCTCGGCCTCGGCCGCGACAAGATCGTGCTGTCGTGCAAGGTGAGCG
GCGTGCAGGACCTGGTCGCCGTGTACCGCGAACTGTCACGCCGCTGCGGCTTCGCGCTGCACCTCGGCCTCACCGAGGCG
GGCATGGGCTCGAAGGGCATCGTCGCGTCGACCGCGGCGATCGGTCTGCTGCTGCAGGAAGGCATCGGCGACACGATCCG
CATCTCGCTCACGCCGGAGCCGGGCGCGCCGCGCACGGGCGAAGTGGTGGTCGGCCAGGAGATCCTGCAGACGATGGGGC
TGCGCTCGTTCGCGCCGATGGTCGTCGCGTGTCCGGGCTGCGGCCGCACGACGAGCACGCTGTTCCAGGAGCTCGCGCTG
CGGATCCAGACCTACCTGCGCGAACAGATGCCCGTGTGGCGCAGCGAATACCCGGGCGTCGAGAAGATGAACGTCGCGGT
GATGGGGTGCATCGTCAACGGCCCGGGCGAGTCGAAGCACGCGAACATCGGCATCAGCCTGCCGGGCTCGGGCGAGAATC
CGGCCGCGCCGGTGTTCGTCGACGGCGAGAAAGTGAAGACGCTGCGCGGCGAGCACATCGCGGAAGAGTTCCAGCAGATC
GTGAGCGACTACGTCGCGCGCACCTACGGCCGCGCCGCGGCGCAGAATTAA

Upstream 100 bases:

>100_bases
TGCGCCGCCGGGCAGGCGGCGCGCTTGGCGTAACTTGGATCTATCGATGCAATCCGAAGCTCAATCCCCACGCAGCAGTC
AGATTTGTTCAACCGAGCCG

Downstream 100 bases:

>100_bases
TTCGTCCACCGTATTCAGATGACAGAACAAAAGCGAAAGCTCGAGAAGCTGACGGGCGTGAAGGGCATGAACGACATCCT
CCCGCAGGATGCCGGCTTGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MFGGHAPRRVSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTADAIGTAIQVKELANAGSELVRITVNTPEAAAAVPAIRE
QLDRMGVTVPLVGDFHYNGHLLLRDYPDCAQALSKYRINPGNVGQGAKRDSQFAQMIEAAIKYDKPVRIGVNWGSLDQDL
LARMMDENGARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLVAVYRELSRRCGFALHLGLTEA
GMGSKGIVASTAAIGLLLQEGIGDTIRISLTPEPGAPRTGEVVVGQEILQTMGLRSFAPMVVACPGCGRTTSTLFQELAL
RIQTYLREQMPVWRSEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFVDGEKVKTLRGEHIAEEFQQI
VSDYVARTYGRAAAQN

Sequences:

>Translated_416_residues
MFGGHAPRRVSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTADAIGTAIQVKELANAGSELVRITVNTPEAAAAVPAIRE
QLDRMGVTVPLVGDFHYNGHLLLRDYPDCAQALSKYRINPGNVGQGAKRDSQFAQMIEAAIKYDKPVRIGVNWGSLDQDL
LARMMDENGARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLVAVYRELSRRCGFALHLGLTEA
GMGSKGIVASTAAIGLLLQEGIGDTIRISLTPEPGAPRTGEVVVGQEILQTMGLRSFAPMVVACPGCGRTTSTLFQELAL
RIQTYLREQMPVWRSEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFVDGEKVKTLRGEHIAEEFQQI
VSDYVARTYGRAAAQN
>Mature_416_residues
MFGGHAPRRVSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTADAIGTAIQVKELANAGSELVRITVNTPEAAAAVPAIRE
QLDRMGVTVPLVGDFHYNGHLLLRDYPDCAQALSKYRINPGNVGQGAKRDSQFAQMIEAAIKYDKPVRIGVNWGSLDQDL
LARMMDENGARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLVAVYRELSRRCGFALHLGLTEA
GMGSKGIVASTAAIGLLLQEGIGDTIRISLTPEPGAPRTGEVVVGQEILQTMGLRSFAPMVVACPGCGRTTSTLFQELAL
RIQTYLREQMPVWRSEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFVDGEKVKTLRGEHIAEEFQQI
VSDYVARTYGRAAAQN

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=382, Percent_Identity=33.7696335078534, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44462; Mature: 44462

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGGHAPRRVSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTADAIGTAIQVKELANAGSE
CCCCCCCHHHHHEEEEEECCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCE
LVRITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPDCAQALSKYRINP
EEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEECCCHHHHHHHHCCCCC
GNVGQGAKRDSQFAQMIEAAIKYDKPVRIGVNWGSLDQDLLARMMDENGARAEPWEAQSV
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
MYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLVAVYRELSRRCGFALHLGLTEA
HHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCHHC
GMGSKGIVASTAAIGLLLQEGIGDTIRISLTPEPGAPRTGEVVVGQEILQTMGLRSFAPM
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECHHHHHHCCCCCCCCE
VVACPGCGRTTSTLFQELALRIQTYLREQMPVWRSEYPGVEKMNVAVMGCIVNGPGESKH
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEEEEECCCCCCCC
ANIGISLPGSGENPAAPVFVDGEKVKTLRGEHIAEEFQQIVSDYVARTYGRAAAQN
CEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFGGHAPRRVSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTADAIGTAIQVKELANAGSE
CCCCCCCHHHHHEEEEEECCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCE
LVRITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPDCAQALSKYRINP
EEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEECCCHHHHHHHHCCCCC
GNVGQGAKRDSQFAQMIEAAIKYDKPVRIGVNWGSLDQDLLARMMDENGARAEPWEAQSV
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
MYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLVAVYRELSRRCGFALHLGLTEA
HHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCHHC
GMGSKGIVASTAAIGLLLQEGIGDTIRISLTPEPGAPRTGEVVVGQEILQTMGLRSFAPM
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECHHHHHHCCCCCCCCE
VVACPGCGRTTSTLFQELALRIQTYLREQMPVWRSEYPGVEKMNVAVMGCIVNGPGESKH
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEEEEECCCCCCCC
ANIGISLPGSGENPAAPVFVDGEKVKTLRGEHIAEEFQQIVSDYVARTYGRAAAQN
CEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA