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The map label for this gene is spoT [H]

Identifier: 126452109

GI number: 126452109

Start: 2948469

End: 2950838

Strand: Reverse

Name: spoT [H]

Synonym: BURPS1106A_3013

Alternate gene names: 126452109

Gene position: 2950838-2948469 (Counterclockwise)

Preceding gene: 126451627

Following gene: 126454976

Centisome position: 73.98

GC content: 64.47

Gene sequence:

>2370_bases
ATGAGCACTACACCATCGTCCGCCTCCTCGGAGGCCGGACACGCCGAGGCCACCGCATCGTCGCACGCGCGTCAATATAT
CGACGCGGTTCTCGAACAATCGTTTCGCCATCTGTTCGGGCCGACCGCGACACCCGAGCAGCCCCGCAAGCACGGCGTCG
TTTCCATCGCGAAACTGACGGCCGCGCTTGCCGATTACCTCGGCGCCGAGGAAATCAAAGAGGTCAAGGCGGCGTTCCAC
TTCAGCGACGAAGCCCACCTCGGTCAATATCGCCAGAGCGGCGAACCCTACATCACCCATCCCGTCGCCGTCGCGGAGAT
CTGCGCCGGCTGGAAGCTCGATGCGCAGGCGATCATGGCGGCGCTGCTGCACGACGTGATGGAAGACCAGGGCGTGACGA
AGAGCGAGCTCGCCGAGCGCTTCGGCCCGAAGGTCGCCGAGCTCGTCGACGGCCTGTCGAAACTCGACAAGATGGAGTTC
AGGAGCCGCGAGGAAGCGCAGGCGGAGAACTTCCGCAAGATGCTGCTCGCGATGGCGCGCGACGTGCGCGTGATCCTCGT
GAAGCTCGCCGATCGCCTGCACAACATGCGCACGCTCGGCGCGGTGCCGATGGAGAAGCGCCGCCGCGTCGCGCGCGAGA
CGCTCGACATCTATGCGCCGATCGCGCACCGCCTCGGTCTGAACAATACGTATCGCGAGCTGCAGGACATGAGCTTCGCG
AACTTCAATCCGCATCGCTATGCGACGCTCGAAAAGGCGGTGAAGGCCGCGCGCGGCAATCGCCGCGAAGTGATCGGCAA
GATTCTCGAATCGGTGCAGCGCGCGATGGCGGATGCGAAGATCGACGCGGAAATCACCGGCCGCGAGAAGACGATCTACA
GCATCTACAAGAAGATGCGCGACAAGCAGTTGTCGTTCTCGCAGGTGCTCGACGTGTACGGCTTCCGGATCGTCGTCGAA
CATCCGCTCGACTGCTACACGTGCATCGGCGTGCTGCATGCGCTTTACAAGCCCGTGCCGGGCAAGTTCAAGGACTACAT
CGCGATCCCGAAGGTCAACGGCTATCAGTCGCTGCACACGACGCTCGTCGGCCCGTTCGGCGCGCCGATCGAGTTCCAGG
TGCGCACGCGCAAGATGCACGAGATCGCGGAAGCGGGCGTTGCCGCGCACTGGCTGTACAAGAACGGCGGCGCGGATCTG
AACGACGTGCAAAAGCGTGCGCATCAATGGCTCAAGTCGCTCCTCGACATCCAGAGCGAGGCGGGCGATTCAAGCGAATT
CCTCGAGCACGTGAAGATCGACCTGTTCCCGGACGCGGTCTACGTGTTCACGCCGAAGTCGAAGATCATGGCGCTGCCGC
GCGGCGCGACCGCGCTCGACTTCGCGTATTCGATCCATAGCGATCTCGGCAATCAGTGCGTCGCCGTGAAGATCAACAAC
GAGCTATTGCCGTTGCGCACCGAATTGAAGAGCGGCGATATCGTCGAGGTGATCACCGCGCCGTACTCGAAGCCGAATCC
CGCATGGCTCGGCTTCGTGCGCACCGGCAAGGCGCGTTCGGCGATCCGGCATTATCTGAAGACGATGCGCCTGAACGAAT
CGGTGCAACTGGGCGAGCGGCTCGTCGATCAGAGCCTGAAGGGCTATGGGCTCGCGCTGGCCGACGTCACGCCCGAGGTC
TGGGAAAAGCTCGTGCAGTGGACGGGCAACAAGAGCCGTCAGGAGATCTTCGCGGACATCGGCCTCGGTCGCCGCGTGGC
CGCCGTGATGGCCAAACGCATCGAGGTGCTGATGAGCGGGCGCGACGCGGACGACGATCTGCCGCGCGCGGAGCGTCATG
CGGCCAACCATGCGCCGCCGGTGGTCATCACCGGCACGGAAGGGATGTCGGTGCAGCTGTCCGCGTGCTGCCGGCCGATT
CCGGGCGACGACATCATGGGCTACATCGGCATCGGGCTCGGGATGGCGATCCACACGACCGCCTGCCGCGTCGCGCAGCG
CATCCACCGGCGCGATCCTGGCCGCTGGATCGACGTCGCCTGGGCGCCGCAGCCGGGCCGCCTGTTCGACGTCGCGGTGA
AGGCGCTCGTGAAGAACACGAAGGGCATCTTCGCGCGCGTCGCGGCCGATATCACGTCGGCGGACGCGAACATCGTCCAT
ATCGCGATGGACGAGGATCTGACGCACGAATCGACGGTGCTGCGCTTCGTGATTCAGGTGAGCGACCGCGTGCACCTCGC
GAACGTGATGCGCCGCGTGCGCACGAATCCGGACGTGATGCGGATCATGCGCGAGCGCTCGAACGACGATGTCGTGCATG
CGCGCCACGATGGCGGAATGCGGATCGATCGCGAGCGGCAGGATTACTAG

Upstream 100 bases:

>100_bases
CGGCGTCGAAATGCTGAAGAAAGTGCCCGCTTAACGCGCGCCCGGTTTCTTCAGCCATGTAGTCCCCCGCGCGACCGACG
AACGAACCCAGGAGGCGAAA

Downstream 100 bases:

>100_bases
CGCCGCGCGGCGCCCGACGGCGAGCGGGCGCGCGCCGGCAATCGCGACGACGCCCTCGGGCCGCCATTTGTTTCGGGCGC
GAAGATGAAATCGGAGGGAA

Product: GTP pyrophosphokinase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 789; Mature: 788

Protein sequence:

>789_residues
MSTTPSSASSEAGHAEATASSHARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALADYLGAEEIKEVKAAFH
FSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEF
RSREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFA
NFNPHRYATLEKAVKAARGNRREVIGKILESVQRAMADAKIDAEITGREKTIYSIYKKMRDKQLSFSQVLDVYGFRIVVE
HPLDCYTCIGVLHALYKPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADL
NDVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINN
ELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVTPEV
WEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPRAERHAANHAPPVVITGTEGMSVQLSACCRPI
PGDDIMGYIGIGLGMAIHTTACRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVH
IAMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSNDDVVHARHDGGMRIDRERQDY

Sequences:

>Translated_789_residues
MSTTPSSASSEAGHAEATASSHARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALADYLGAEEIKEVKAAFH
FSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEF
RSREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFA
NFNPHRYATLEKAVKAARGNRREVIGKILESVQRAMADAKIDAEITGREKTIYSIYKKMRDKQLSFSQVLDVYGFRIVVE
HPLDCYTCIGVLHALYKPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADL
NDVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINN
ELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVTPEV
WEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPRAERHAANHAPPVVITGTEGMSVQLSACCRPI
PGDDIMGYIGIGLGMAIHTTACRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVH
IAMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSNDDVVHARHDGGMRIDRERQDY
>Mature_788_residues
STTPSSASSEAGHAEATASSHARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALADYLGAEEIKEVKAAFHF
SDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFR
SREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
FNPHRYATLEKAVKAARGNRREVIGKILESVQRAMADAKIDAEITGREKTIYSIYKKMRDKQLSFSQVLDVYGFRIVVEH
PLDCYTCIGVLHALYKPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLN
DVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVTPEVW
EKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPRAERHAANHAPPVVITGTEGMSVQLSACCRPIP
GDDIMGYIGIGLGMAIHTTACRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSNDDVVHARHDGGMRIDRERQDY

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=710, Percent_Identity=45.6338028169014, Blast_Score=661, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=31.6251830161054, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 88042; Mature: 87911

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPSSASSEAGHAEATASSHARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHH
AALADYLGAEEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMA
HHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHH
ALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMAR
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
NFNPHRYATLEKAVKAARGNRREVIGKILESVQRAMADAKIDAEITGREKTIYSIYKKMR
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHH
DKQLSFSQVLDVYGFRIVVEHPLDCYTCIGVLHALYKPVPGKFKDYIAIPKVNGYQSLHT
HCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHH
TLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLDIQSE
HHCCCCCCCEEEEHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
AGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINN
CCCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECC
ELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGER
CEECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHH
LVDQSLKGYGLALADVTPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSG
HHHHHHHCCCEEHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
RDADDDLPRAERHAANHAPPVVITGTEGMSVQLSACCRPIPGDDIMGYIGIGLGMAIHTT
CCCCCCCCHHHHHHCCCCCCEEEECCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ACRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVH
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IAMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSNDDVVHARHDGGM
EEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCC
RIDRERQDY
EECCCCCCC
>Mature Secondary Structure 
STTPSSASSEAGHAEATASSHARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHH
AALADYLGAEEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAIMA
HHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHH
ALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMAR
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
NFNPHRYATLEKAVKAARGNRREVIGKILESVQRAMADAKIDAEITGREKTIYSIYKKMR
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHH
DKQLSFSQVLDVYGFRIVVEHPLDCYTCIGVLHALYKPVPGKFKDYIAIPKVNGYQSLHT
HCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHH
TLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLDIQSE
HHCCCCCCCEEEEHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
AGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINN
CCCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECC
ELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGER
CEECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHH
LVDQSLKGYGLALADVTPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSG
HHHHHHHCCCEEHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
RDADDDLPRAERHAANHAPPVVITGTEGMSVQLSACCRPIPGDDIMGYIGIGLGMAIHTT
CCCCCCCCHHHHHHCCCCCCEEEECCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ACRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVH
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IAMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSNDDVVHARHDGGM
EEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCC
RIDRERQDY
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]