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The map label for this gene is purH [H]

Identifier: 126452027

GI number: 126452027

Start: 3315897

End: 3317462

Strand: Direct

Name: purH [H]

Synonym: BURPS1106A_3398

Alternate gene names: 126452027

Gene position: 3315897-3317462 (Clockwise)

Preceding gene: 126451778

Following gene: 126453395

Centisome position: 83.14

GC content: 66.79

Gene sequence:

>1566_bases
ATGATCAAGCAAGCGCTCATTTCCGTTTCCGACAAGACCGGCATCGTCGACTTCGCGAAAGCGCTGTCCGCGCTCGGCGT
CAAGCTGCTGTCGACGGGCGGCACCGCGAAACTGCTCGCCGACGCGGGCCTGCCCGTCACCGAAGTGGCCGACTACACCG
GCTTCCCGGAAATGCTCGATGGGCGCGTGAAGACGCTGCACCCGAAGGTGCACGGCGGCATCCTCGCCCGCCGCGACCTG
CCCGAGCACATGCAGGCGCTCGAAGCGCACGGGATTCCGACGATCGACCTGCTCGTCGTGAACCTGTATCCGTTCGTCCA
GACGATTGCGAAGGACGACTGCACGCTCGCCGACGCGATCGAGAACATCGACATCGGCGGCCCGACGATGCTGCGCTCGG
CGGCGAAGAACCACCGCGACGTGACGGTCGTCGTCGACCCGGCCGATTACGCGGTCGTGCTCGACGAGATGAAAGCGAAC
GGCAACACGCTCGGCTACAAGACGAATTTCCGCCTCGCGACCAAGGTGTTCGCGCACACCGCGCAGTACGACGGCGCGAT
CACGAACTACCTGACGAGCCTCGGCGACGATCTGCAGCACGGCTCGCGCAGCGCATACCCGGCAACGCTGAACCTCGCGT
TCGACAAGGTGCAGGACCTGCGCTACGGCGAGAATCCGCACCAGAGCGCCGCGTTCTACCGCGACGTCGCGACGCCGGCC
GGCGCGCTCGCGAACTACCGCCAGTTGCAGGGCAAGGAACTGTCGTACAACAACATCGCCGATTCGGACGCCGCGTGGGA
ATGCGTGAAGACGTTCGACGCGCCGGCGTGCGTGATCATCAAGCACGCGAATCCGTGCGGCGTCGCGGTGGGCGCGGACG
CGGGCGAAGCGTACGCGAAGGCGTTCCAGACCGATCCGACCTCCGCGTTCGGCGGCATCATCGCGTTCAACCGCGAAGTC
GACGAGGCCGCGGCCCAGGCGGTCGCGAAGCAATTCGTCGAAGTGCTGATCGCGCCGTCGTTCTCGGACGCGGCCAAGCA
GGTGTTCGCGGCCAAGCAGAACGTGCGCCTGCTCGAAATCGCGCTGGGCGAAGGCCATAACGCGTTCGATCTGAAGCGCG
TGGGCGGCGGCCTGCTCGTGCAATCGCTCGATTCGAAGAACGTGCAGCCGCGCGAGCTGCGCGTCGTCACGAAACGCCAC
CCGACGCCGAAGGAAATGGACGACCTCCTGTTCGCATGGCGCGTCGCGAAATACGTGAAGTCGAACGCGATCGTGTTCTG
CGGCAACGGGATGACGCTCGGCGTCGGCGCAGGCCAGATGAGCCGCGTCGATTCGGCGCGCATCGCGAGCATCAAGGCAC
AGAACGCGGGCCTCACGCTCGCGGGCTCGGCCGTCGCATCGGACGCGTTCTTCCCGTTCCGCGACGGCCTCGACGTCGTC
GTCGCGGCGGGCGCGACCTGCGTGATCCAGCCGGGCGGCTCGGTGCGCGACGACGAGGTGATCGCCGCCGCCGACGAGCA
CAACATCGCGATGGTCGTGACGGGCGTGCGCCACTTCCGTCACTGA

Upstream 100 bases:

>100_bases
TCGGCATCAATCGCAACACGCTGCGCAAGAAGCTGCAGCAGCACGGCCTGCTGTAGGCGGCCGCCCCGTCCCCGTTTTCC
TGGCTATTGGTGGTTCCATC

Downstream 100 bases:

>100_bases
TTCGTACGACGGACGCATCGGTTTCGGCCAAAGCCGGCGGTCGCTGACCGCCGTTTTTTTTGGAGGCCGCGCGACGGCGA
TGCGCGATGCGGCGGTCGTT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MIKQALISVSDKTGIVDFAKALSALGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEAHGIPTIDLLVVNLYPFVQTIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN
GNTLGYKTNFRLATKVFAHTAQYDGAITNYLTSLGDDLQHGSRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDVATPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGADAGEAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDSKNVQPRELRVVTKRH
PTPKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMVVTGVRHFRH

Sequences:

>Translated_521_residues
MIKQALISVSDKTGIVDFAKALSALGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEAHGIPTIDLLVVNLYPFVQTIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN
GNTLGYKTNFRLATKVFAHTAQYDGAITNYLTSLGDDLQHGSRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDVATPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGADAGEAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDSKNVQPRELRVVTKRH
PTPKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMVVTGVRHFRH
>Mature_521_residues
MIKQALISVSDKTGIVDFAKALSALGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEAHGIPTIDLLVVNLYPFVQTIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN
GNTLGYKTNFRLATKVFAHTAQYDGAITNYLTSLGDDLQHGSRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDVATPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGADAGEAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDSKNVQPRELRVVTKRH
PTPKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMVVTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=482, Percent_Identity=37.551867219917, Blast_Score=275, Evalue=8e-74,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=62.4761904761905, Blast_Score=654, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=612, Percent_Identity=32.843137254902, Blast_Score=290, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71985574, Length=318, Percent_Identity=25.7861635220126, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=33.8461538461538, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=36.0416666666667, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6323056, Length=489, Percent_Identity=35.9918200408998, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24649832, Length=486, Percent_Identity=38.8888888888889, Blast_Score=276, Evalue=3e-74,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55459; Mature: 55459

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQALISVSDKTGIVDFAKALSALGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHHEECCCCCCHHHHHHHHHHHCHHEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMQALEAHGIPTIDLLVVNLYPFVQTIAKDDCTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKANGNTLGYKTNFRLATKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCEEEEECCCHHHHHHHHHH
AQYDGAITNYLTSLGDDLQHGSRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDVATPA
HHCCCHHHHHHHHHCHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGADAGEAYAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCHHHHH
AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE
ALGEGHNAFDLKRVGGGLLVQSLDSKNVQPRELRVVTKRHPTPKEMDDLLFAWRVAKYVK
EECCCCCCEEHHHCCCCCEEEECCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
CCEEEEECCCEEEEECCCCHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMVVTGVRHFRH
EECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECHHHHCC
>Mature Secondary Structure
MIKQALISVSDKTGIVDFAKALSALGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHHEECCCCCCHHHHHHHHHHHCHHEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMQALEAHGIPTIDLLVVNLYPFVQTIAKDDCTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKANGNTLGYKTNFRLATKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCEEEEECCCHHHHHHHHHH
AQYDGAITNYLTSLGDDLQHGSRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDVATPA
HHCCCHHHHHHHHHCHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGADAGEAYAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCHHHHH
AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE
ALGEGHNAFDLKRVGGGLLVQSLDSKNVQPRELRVVTKRHPTPKEMDDLLFAWRVAKYVK
EECCCCCCEEHHHCCCCCEEEECCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
CCEEEEECCCEEEEECCCCHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE
VAAGATCVIQPGGSVRDDEVIAAADEHNIAMVVTGVRHFRH
EECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA