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The map label for this gene is gltD [H]

Identifier: 126451972

GI number: 126451972

Start: 3654427

End: 3655893

Strand: Reverse

Name: gltD [H]

Synonym: BURPS1106A_3746

Alternate gene names: 126451972

Gene position: 3655893-3654427 (Counterclockwise)

Preceding gene: 126454893

Following gene: 126453082

Centisome position: 91.66

GC content: 67.76

Gene sequence:

>1467_bases
ATGGGCAAGATCACCGGTTTTCTGGAGTACGAGCGCCGTCACGAGGCGTACGAGGCGCCGCTCACGCGCGTGAAGCACTA
CAAGGAGTTCGTCGCGGCGCTGACCGACGCGGACGCGAAGATCCAGGGCGCACGCTGCATGGATTGCGGCATTCCGTTTT
GCAACAACGGCTGCCCCGTCAACAACATCATTCCGGACTTCAACGATCTCGTGTATCGCCAGGAATGGCGTCAGGCGATC
GACGTGCTGCATTCGACGAACAACTTCCCGGAGTTCACGGGCCGCATCTGCCCGGCGCCGTGCGAAGCGGCGTGCACGCT
CGGCATCCATGACGATCCGGTCGGCATCAAGTCGATCGAGCACGCGATCATCGACAAGGCATGGGCGCAGGGCTGGGTCG
CCCCGCAGCCCGCCGCGCACAAGACGGGCAAGAAGGTCGCGGTGGTCGGCTCGGGCCCCGCGGGCCTTGCCGCCGCGCAG
CAGCTCGCGCGCGCGGGCCACGACGTGACGGTGTTCGAGAAGAGCGACCGGATCGGCGGCCTGCTGCGTTACGGGATTCC
CGATTTCAAGCTCGAGAAGTGGCTGATCGACCGCCGGATGCGCCAGATGGAAGCGGAAGGCGTGACGTTCCGCACGAGCG
TGTTCGTCGGCCGCGATCCGCTGCCCGAGACGATCGGCAACATGGCGAAGGAGACGATTTCGCCCGAGACGCTGAAGGAC
GAGTTCGACGCGGTCGTGATCGCGGGCGGCTCGGAGACGCCGCGCGATCTGCCGGTGCCGGGGCGCGAGCTCGCCGGCAT
CCATTTCGCGATGGAGTTCCTGCCGCAGCAGAACCGCGTGAACGCGGGCGACAAGCTGGCCGATCAACTGCTCGCGAAGG
GCAAGCACGTCGTCGTGATCGGCGGCGGCGACACGGGTTCGGATTGCGTCGGCACGTCGAACCGCCACGGCGCGAAGCAC
GTCACGCAGTTCGAGCTGCTGCCGCAGCCGCCCGAGGCGGAGAACAAGCCGCTCGTGTGGCCGTACTGGCCGATCAAGCT
GCGCACGTCGTCGTCGCACGAGGAAGGCTGCGAGCGCGACTGGGCGGTGGCGACGAAACGTTTCGAAGGCAGGAACGGCA
AGGTCGAGAAGCTGATCGCGGCGCGCGTCGCGTGGGTCGACGGCAAGATGCAGGAAGTGCCGGATTCCGAGTTCGAGATC
AAGGCCGACCTCGTGCTGCTCGCGATGGGTTTCACGCAGCCGGCCGCGCCCGTGCTCGAGGCGTTCGGCGTCGCGAAGGA
CGCGCGCGGCAACGCGCGCGCGGGCACCGAGGGCGATCGCGCGTACTACACGTCGGTCGACAAGGTGTTCGCCGCGGGCG
ACATGCGCCGCGGCCAGTCGCTCGTCGTCTGGGCGATCCGCGAAGGCCGCCAGTGCGCGCGCTCGGTCGACGCGTACCTG
ATGGGCAGCTCGGAGTTGCCGCGCTGA

Upstream 100 bases:

>100_bases
CGCCTGACGCAACGCGGCTCGACGACATAGCGAACGCGCCCGTCCGATGGGCGGGCGCGGGTCCCCCTTACCCGATTCAA
CAGATTCAGAAGAGAACCTC

Downstream 100 bases:

>100_bases
GCGGACGGCAGTCGCCAGAGCGCCGTTTCGACAAACCGGGCGTCCCGCGGGCGCCCGGTTTTTTTTACGCGGCCGCTTTG
CCGCGGCGCGCATCGCGCGC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MGKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQEWRQAI
DVLHSTNNFPEFTGRICPAPCEAACTLGIHDDPVGIKSIEHAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKSDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFVGRDPLPETIGNMAKETISPETLKD
EFDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKLADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKH
VTQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRFEGRNGKVEKLIAARVAWVDGKMQEVPDSEFEI
KADLVLLAMGFTQPAAPVLEAFGVAKDARGNARAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGSSELPR

Sequences:

>Translated_488_residues
MGKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQEWRQAI
DVLHSTNNFPEFTGRICPAPCEAACTLGIHDDPVGIKSIEHAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKSDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFVGRDPLPETIGNMAKETISPETLKD
EFDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKLADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKH
VTQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRFEGRNGKVEKLIAARVAWVDGKMQEVPDSEFEI
KADLVLLAMGFTQPAAPVLEAFGVAKDARGNARAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGSSELPR
>Mature_487_residues
GKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQEWRQAID
VLHSTNNFPEFTGRICPAPCEAACTLGIHDDPVGIKSIEHAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQQ
LARAGHDVTVFEKSDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFVGRDPLPETIGNMAKETISPETLKDE
FDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKLADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKHV
TQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRFEGRNGKVEKLIAARVAWVDGKMQEVPDSEFEIK
ADLVLLAMGFTQPAAPVLEAFGVAKDARGNARAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYLM
GSSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=488, Percent_Identity=24.1803278688525, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1789606, Length=486, Percent_Identity=34.5679012345679, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788811, Length=467, Percent_Identity=34.6895074946467, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082180, Length=470, Percent_Identity=34.2553191489362, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1788468, Length=463, Percent_Identity=27.8617710583153, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789243, Length=445, Percent_Identity=27.6404494382022, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=506, Percent_Identity=45.2569169960474, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71984108, Length=482, Percent_Identity=25.3112033195021, Blast_Score=95, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6320030, Length=502, Percent_Identity=48.605577689243, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI28574881, Length=503, Percent_Identity=49.7017892644135, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24665539, Length=503, Percent_Identity=49.7017892644135, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24665547, Length=503, Percent_Identity=49.7017892644135, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24665543, Length=503, Percent_Identity=49.7017892644135, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24640763, Length=482, Percent_Identity=24.0663900414938, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI18858217, Length=482, Percent_Identity=24.0663900414938, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53499; Mature: 53368

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQEWRQAIDVLHSTNNFPEFTGRICPAPCEAACTLGIHDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQQLARAGHDVTVFEKSDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFVGRDPLPETIGNMAKETISPETLKD
HHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHC
EFDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKLADQLLAKGKHVVVI
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKHVTQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRFEGRNGKVEKLIAARVAWVDGKMQEVPDSEFEIKADLVLLAMGFTQPAAPVLE
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCCEEEEEEEEEEEECCCCCHHHHHH
AFGVAKDARGNARAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHE
MGSSELPR
ECCCCCCC
>Mature Secondary Structure 
GKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQEWRQAIDVLHSTNNFPEFTGRICPAPCEAACTLGIHDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQQLARAGHDVTVFEKSDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFVGRDPLPETIGNMAKETISPETLKD
HHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHC
EFDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKLADQLLAKGKHVVVI
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKHVTQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRFEGRNGKVEKLIAARVAWVDGKMQEVPDSEFEIKADLVLLAMGFTQPAAPVLE
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCCEEEEEEEEEEEECCCCCHHHHHH
AFGVAKDARGNARAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHE
MGSSELPR
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]