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The map label for this gene is hisF [H]

Identifier: 126451649

GI number: 126451649

Start: 3631226

End: 3632014

Strand: Reverse

Name: hisF [H]

Synonym: BURPS1106A_3718

Alternate gene names: 126451649

Gene position: 3632014-3631226 (Counterclockwise)

Preceding gene: 126452514

Following gene: 126454802

Centisome position: 91.06

GC content: 69.96

Gene sequence:

>789_bases
ATGCCGCGCAACATCATGGCTCTAGCTAAACGCATCATCCCCTGTCTCGACGTGACCGCCGGGCGCGTCGTCAAGGGCGT
CAATTTCGTCGAGCTGCGCGACGCGGGAGACCCCGTCGAGATCGCGCGGCGCTATGACGCCCAGGGCGCGGACGAACTCA
CGTTCCTCGACATCACCGCGACGTCGGACGGCCGCGACCTGATCCTGCCGATCATCGAGGCGGTCGCGTCGCAGGTGTTC
ATCCCGCTCACGGTGGGCGGCGGCGTGCGCGCGGTCGAGGACGTGCGGCGCCTCCTGAACGCCGGCGCGGACAAGGTCAG
CATGAACTCGTCGGCGGTCGCCAATCCGCCGCTCGTGCGCGACGCGGCCGATAAGTACGGCTCGCAGTGCATCGTCGTCG
CGATCGACGCGAAGCGCGTGTCGGCCGACGGCGAGCCGCCGCGCTGGGAAGTCTTCACGCACGGCGGCCGCAAGGGCACG
GGCCTCGACGCGGTCGAATGGGCGCGCAAGATGGCCGAGCTCGGCGCGGGCGAGATCCTGCTCACGAGCATGGACCGCGA
CGGCACGAAGGCGGGTTTCGATCTCGCGCTCACGCGCGCGGTGTCGGATGCGGTGCCGGTGCCCGTGATCGCGTCGGGCG
GCGTCGGCTCGCTCGAGCATCTCGCGGCGGGCATCACCGAAGGCCACGCGGACGCCGTGCTCGCCGCGAGCATCTTCCAT
TACGGCGAGCACACGGTCGGCGAGGCGAAGCGCTTCATGGCCGAGCGCGGCATTGCAGTGAGGTTGTGA

Upstream 100 bases:

>100_bases
GACGAACTGAACGGCGAGCTCGACGACGCCTGACGCGCCGTCTCGGCACGACGCCTTTTCGCCAAGCCGTCCGCCTCGCG
CGGGCGGCATTACCCGCGGC

Downstream 100 bases:

>100_bases
TGAATGCAGAAGCGAAGCCGGGCGACTGGCTGGGCAAGGTCCGCTGGGATGCGAACGGCCTCGTGCCGGTGATCGCGCAG
GACGCGGCGACGAACGACGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGRDLILPIIEAVASQVF
IPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGT
GLDAVEWARKMAELGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFH
YGEHTVGEAKRFMAERGIAVRL

Sequences:

>Translated_262_residues
MPRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGRDLILPIIEAVASQVF
IPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGT
GLDAVEWARKMAELGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFH
YGEHTVGEAKRFMAERGIAVRL
>Mature_261_residues
PRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGRDLILPIIEAVASQVFI
PLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTG
LDAVEWARKMAELGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHY
GEHTVGEAKRFMAERGIAVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=42.8571428571429, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=32.2314049586777, Blast_Score=105, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=35.4430379746835, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27591; Mature: 27460

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITA
CCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEE
TSDGRDLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVR
CCCCCCHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHH
DAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTGLDAVEWARKMAELGAGEIL
HHHHHHCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEE
LTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFH
EEECCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHH
YGEHTVGEAKRFMAERGIAVRL
HCCCCHHHHHHHHHHCCCEEEC
>Mature Secondary Structure 
PRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITA
CCHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEE
TSDGRDLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVR
CCCCCCHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHH
DAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTGLDAVEWARKMAELGAGEIL
HHHHHHCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEE
LTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFH
EEECCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHH
YGEHTVGEAKRFMAERGIAVRL
HCCCCHHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA