Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 126451553

GI number: 126451553

Start: 1070612

End: 1072312

Strand: Direct

Name: nadE [H]

Synonym: BURPS1106A_1089

Alternate gene names: 126451553

Gene position: 1070612-1072312 (Clockwise)

Preceding gene: 126454730

Following gene: 126454900

Centisome position: 26.84

GC content: 69.66

Gene sequence:

>1701_bases
ATGAAGACCCGTATCGCACTCGCCCAACTCAACGTCACCGTCGGCGATTTCGCCGGCAACGTCGCCAAGATCGTCGCCGC
CGCGCAGGCCGCGCACGATGCCGGCGCGCACCTGCTGATCGCGCCCGAGCTCGCGCTGTCCGGCTACCCGCCCGAGGATC
TGCTGCTGCGGCCCGCGTTCTACGCGGCGTCCGACGCGGCGCTCGCCGCGCTCGCCGCGCAACTGAAGCCGCTCGCCGGG
CTCGCGGTGCTCGTCGGCCATCCGCTGCGTGCGGCCGGCGCGGGCGCGTCAAGCGCCGATGGTAATGCAAACCGTCCGAT
CGAGCGCGGCGCCGCGCCGGTCGACACCTACAACGCGGCGTCGCTGATCGTCGGCGGCGAGGTGGTCGGCACGTACCGCA
AGCAGGATCTGCCGAACACCGAGGTGTTCGACGAGAAGCGCTATTTCGCGACCGACGCGGCGCCTTACGTGTTCGAGCTG
AACGGCGTGCGCTTCGGCGTCGTGATCTGCGAGGACGTGTGGCACGCGTCGGCCGCGCAGCTCGCGAAGGCGGCCGGCGC
GCAGGTGCTGATCGTGCCGAACGGCTCGCCGTATCACATGCACAAGGAGGCGGTGCGCATCGACATCCTGCGCGCGCGGA
TTCGCGAAACGGGCCTGCCGATGGTCTACGTGAATCTCGTCGGCGGCCAGGACGAGCTCGTGTTCGACGGCGGCTCGTTC
GTGCTCGACGGCGCGGGCGAGCTCGTCGCGAAGATGCCGCAGTTCGAGGAGGGCCATGCGATCGTCGAATTCGACGGCGC
GCGGGCGCTGCCCGCGCCGATCGCGCCGGCGCTGCCGGTCGAGGCGCAGGTGTACCGCGCGCTCGTGCTCGGCGTGCGCG
ACTATATCGGCAAGAACGGCTTCCCGGGCGCGATCGTCGGGCTGTCGGGCGGCGTCGATTCGGCGCTCGTGCTCGCGGTC
GCCGTCGACGCGCTCGGCGCCGAGCGCGTGCGCGCCGTGATGATGCCGTCGCGCTACACGGCCGACATCTCGACGACCGA
TGCGGCCGACATGGCAAAGCGCGTCGGCGTGCGCTACGACGAGATCGCGATCGCGCCGATGTTCGACGCGTTCCGCGCGT
CGCTCGCGGCCGAATTCGCGGGCCTCGCCGAAGACGCGACGGAGGAGAACATCCAGGCGCGCATTCGCGGCACGCTGCTG
ATGGCGCTGTCGAACAAGTTCGGCTCGATCGTGCTGACGACGGGCAACAAGAGCGAGATGGCGGTCGGCTACTGCACGCT
GTACGGCGACATGGCGGGCGGCTTCGCGGTGATCAAGGACATCGCGAAGACGCTCGTCTACCGGCTCTGCCGCTATCGCA
ACGCGGCGGCGGAATACGGCGCGCTCGACGTCATTCCCGAGCGGATCCTCACGCGCGCGCCGTCGGCCGAGCTGCGCGAG
AACCAGACCGATCAGGACAGCCTGCCGCCGTACGACGTGCTCGATGCGATCATGCGCATGTACATGGAGGAGGACCGGCC
GCTCGCGGAGATCGTCGCGGCGGGCTATGCGGAGGCGGACGTGAAGCGCGTCACGCGGCTCATCAAGATCAACGAGTACA
AGCGCCGCCAGGCGCCCGTCGGCATTCGCGTCACGCATCGCGCGTTCGGGCGCGACTGGCGCTATCCGATCACGTCGCGC
TTCATCGAGAGCATCGACTGA

Upstream 100 bases:

>100_bases
CGTACCCGTCTGCACGGCCGGCTGCGCGCGCTGCCGCGCCGCCCGTGCGTTTGCCAAAGCCGTTCGCGCCGTGGCCCGAG
GAGGCCGCGCTGATCCCGTG

Downstream 100 bases:

>100_bases
CGCGGCGCGCGGCGTAGAATCGGCTGACGGTTTTCACTCATTCGAAGCAAGGGGCACATCATGAAACGCATCACCGCCAT
CATCAAGCCGTTCAAGCTGG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHLLIAPELALSGYPPEDLLLRPAFYAASDAALAALAAQLKPLAG
LAVLVGHPLRAAGAGASSADGNANRPIERGAAPVDTYNAASLIVGGEVVGTYRKQDLPNTEVFDEKRYFATDAAPYVFEL
NGVRFGVVICEDVWHASAAQLAKAAGAQVLIVPNGSPYHMHKEAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSF
VLDGAGELVAKMPQFEEGHAIVEFDGARALPAPIAPALPVEAQVYRALVLGVRDYIGKNGFPGAIVGLSGGVDSALVLAV
AVDALGAERVRAVMMPSRYTADISTTDAADMAKRVGVRYDEIAIAPMFDAFRASLAAEFAGLAEDATEENIQARIRGTLL
MALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGALDVIPERILTRAPSAELRE
NQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAEADVKRVTRLIKINEYKRRQAPVGIRVTHRAFGRDWRYPITSR
FIESID

Sequences:

>Translated_566_residues
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHLLIAPELALSGYPPEDLLLRPAFYAASDAALAALAAQLKPLAG
LAVLVGHPLRAAGAGASSADGNANRPIERGAAPVDTYNAASLIVGGEVVGTYRKQDLPNTEVFDEKRYFATDAAPYVFEL
NGVRFGVVICEDVWHASAAQLAKAAGAQVLIVPNGSPYHMHKEAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSF
VLDGAGELVAKMPQFEEGHAIVEFDGARALPAPIAPALPVEAQVYRALVLGVRDYIGKNGFPGAIVGLSGGVDSALVLAV
AVDALGAERVRAVMMPSRYTADISTTDAADMAKRVGVRYDEIAIAPMFDAFRASLAAEFAGLAEDATEENIQARIRGTLL
MALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGALDVIPERILTRAPSAELRE
NQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAEADVKRVTRLIKINEYKRRQAPVGIRVTHRAFGRDWRYPITSR
FIESID
>Mature_566_residues
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHLLIAPELALSGYPPEDLLLRPAFYAASDAALAALAAQLKPLAG
LAVLVGHPLRAAGAGASSADGNANRPIERGAAPVDTYNAASLIVGGEVVGTYRKQDLPNTEVFDEKRYFATDAAPYVFEL
NGVRFGVVICEDVWHASAAQLAKAAGAQVLIVPNGSPYHMHKEAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSF
VLDGAGELVAKMPQFEEGHAIVEFDGARALPAPIAPALPVEAQVYRALVLGVRDYIGKNGFPGAIVGLSGGVDSALVLAV
AVDALGAERVRAVMMPSRYTADISTTDAADMAKRVGVRYDEIAIAPMFDAFRASLAAEFAGLAEDATEENIQARIRGTLL
MALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGALDVIPERILTRAPSAELRE
NQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAEADVKRVTRLIKINEYKRRQAPVGIRVTHRAFGRDWRYPITSR
FIESID

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60518; Mature: 60518

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHLLIAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEHHHHCCHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCHHHEECCHH
YAASDAALAALAAQLKPLAGLAVLVGHPLRAAGAGASSADGNANRPIERGAAPVDTYNAA
HCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
SLIVGGEVVGTYRKQDLPNTEVFDEKRYFATDAAPYVFELNGVRFGVVICEDVWHASAAQ
EEEECCHHEECHHHCCCCCCCCCCCCCCEECCCCCEEEEECCEEEEEEEECHHHHHHHHH
LAKAAGAQVLIVPNGSPYHMHKEAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSF
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCE
VLDGAGELVAKMPQFEEGHAIVEFDGARALPAPIAPALPVEAQVYRALVLGVRDYIGKNG
EECCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
FPGAIVGLSGGVDSALVLAVAVDALGAERVRAVMMPSRYTADISTTDAADMAKRVGVRYD
CCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHCCCCCEECCCCCCCHHHHHHHHCCCCH
EIAIAPMFDAFRASLAAEFAGLAEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKSEM
HEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCE
AVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGALDVIPERILTRAPSAELRE
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHHC
NQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAEADVKRVTRLIKINEYKRRQAPV
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIRVTHRAFGRDWRYPITSRFIESID
CEEEEHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKTRIALAQLNVTVGDFAGNVAKIVAAAQAAHDAGAHLLIAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEHHHHCCHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCHHHEECCHH
YAASDAALAALAAQLKPLAGLAVLVGHPLRAAGAGASSADGNANRPIERGAAPVDTYNAA
HCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
SLIVGGEVVGTYRKQDLPNTEVFDEKRYFATDAAPYVFELNGVRFGVVICEDVWHASAAQ
EEEECCHHEECHHHCCCCCCCCCCCCCCEECCCCCEEEEECCEEEEEEEECHHHHHHHHH
LAKAAGAQVLIVPNGSPYHMHKEAVRIDILRARIRETGLPMVYVNLVGGQDELVFDGGSF
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCE
VLDGAGELVAKMPQFEEGHAIVEFDGARALPAPIAPALPVEAQVYRALVLGVRDYIGKNG
EECCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
FPGAIVGLSGGVDSALVLAVAVDALGAERVRAVMMPSRYTADISTTDAADMAKRVGVRYD
CCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHCCCCCEECCCCCCCHHHHHHHHCCCCH
EIAIAPMFDAFRASLAAEFAGLAEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKSEM
HEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCE
AVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNAAAEYGALDVIPERILTRAPSAELRE
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHHC
NQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAEADVKRVTRLIKINEYKRRQAPV
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIRVTHRAFGRDWRYPITSRFIESID
CEEEEHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]