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The map label for this gene is dosP [H]

Identifier: 126451509

GI number: 126451509

Start: 2069262

End: 2070056

Strand: Reverse

Name: dosP [H]

Synonym: BURPS1106A_2076

Alternate gene names: 126451509

Gene position: 2070056-2069262 (Counterclockwise)

Preceding gene: 126454519

Following gene: 126452455

Centisome position: 51.9

GC content: 65.28

Gene sequence:

>795_bases
TTGGACGAGGCCGATCTCGAGCGGGCATTCGAGGCCGGAGAATTCGTCATGGATTATCAGCCGATCGTCTCGATCCGCTC
CGGGGCCGTGACGGGCGCCGAAGCGACGATACGATGGGATCATCCGGAATGGGGCGCGCTGCCTCAGCGTGTGGTCCACG
CCGCCGCGGACCGATTGGGCGCGGCGTCCCGGATCGCCGGCCACGCGTTCGGCGAGGCGTGCAGGCAGCTCGCGCGCTGG
AAGCAGCAGGGCGCCGGCGTGTCGGCGCTGTCGATGCGGCTTTCGGGCGCCCAGTTGGGCGCCGAAAGCGTATTCGAGCG
ACTCGCGCTGTCGATCGAGGCGTTCGATATCTCGAGCAGCCAGTTCACGCTCGAAGTGCCGGAAATGTCGGCGCCGGAGG
AATCGCTGAGTCTTCTCGATCGCCTGAAGCGGCTGAGGCAGAAGGGTTACGGCATCGTGCTCGGCGATTTCGGAGCGCAT
CACACGGCGATGTCGACGTTGATGGTGCTGCCCGTCACGGGCGTCAAATTCGGCGAATCGTTCACGGAGCGATTGCCCGG
TTCGCCGACTGCGGAGGCGATCCTGTCGAGCGTCTCGCGTCTCGCGCACGATCTGGGTTTCACGTTGACGGTGTCCGGCG
TCGAAAACGGCCGGCAGTTCGAATTGCTGCGTCGCTTTCGCGATATCGAGCTGCAGGGCGCCTTTCTGTTCGAGCCGATG
CGCGCCGAGGTGTGGCACGAGCGCGTGAATCCGAGAGCGCTGTCGCAACTGAATCTGCGTGAGCCGCCGTTCTGA

Upstream 100 bases:

>100_bases
GATGCTGCGACGGCGGCGTCTTTGGGAGAGCGATATGGAAGCGGGCATCACGCGTCGTAGGGGTGCATTCTTTGCCCGGG
AACACGAACACGGACTGTCG

Downstream 100 bases:

>100_bases
CGCGGCGAGCCTGGCCGCTCGCGTTTCATCCCGAGGTGCGGGATCGGGCACGCGTGCGGCCGGCCCGCCCGCATGGCGAG
CCGGGTTGCGCAGCCGCCGC

Product: cyclic diguanylate phosphodiesterase

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDEADLERAFEAGEFVMDYQPIVSIRSGAVTGAEATIRWDHPEWGALPQRVVHAAADRLGAASRIAGHAFGEACRQLARW
KQQGAGVSALSMRLSGAQLGAESVFERLALSIEAFDISSSQFTLEVPEMSAPEESLSLLDRLKRLRQKGYGIVLGDFGAH
HTAMSTLMVLPVTGVKFGESFTERLPGSPTAEAILSSVSRLAHDLGFTLTVSGVENGRQFELLRRFRDIELQGAFLFEPM
RAEVWHERVNPRALSQLNLREPPF

Sequences:

>Translated_264_residues
MDEADLERAFEAGEFVMDYQPIVSIRSGAVTGAEATIRWDHPEWGALPQRVVHAAADRLGAASRIAGHAFGEACRQLARW
KQQGAGVSALSMRLSGAQLGAESVFERLALSIEAFDISSSQFTLEVPEMSAPEESLSLLDRLKRLRQKGYGIVLGDFGAH
HTAMSTLMVLPVTGVKFGESFTERLPGSPTAEAILSSVSRLAHDLGFTLTVSGVENGRQFELLRRFRDIELQGAFLFEPM
RAEVWHERVNPRALSQLNLREPPF
>Mature_264_residues
MDEADLERAFEAGEFVMDYQPIVSIRSGAVTGAEATIRWDHPEWGALPQRVVHAAADRLGAASRIAGHAFGEACRQLARW
KQQGAGVSALSMRLSGAQLGAESVFERLALSIEAFDISSSQFTLEVPEMSAPEESLSLLDRLKRLRQKGYGIVLGDFGAH
HTAMSTLMVLPVTGVKFGESFTERLPGSPTAEAILSSVSRLAHDLGFTLTVSGVENGRQFELLRRFRDIELQGAFLFEPM
RAEVWHERVNPRALSQLNLREPPF

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=240, Percent_Identity=31.25, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787541, Length=254, Percent_Identity=24.8031496062992, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081845, Length=238, Percent_Identity=26.0504201680672, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790496, Length=258, Percent_Identity=26.3565891472868, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI226510982, Length=254, Percent_Identity=27.1653543307087, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081743, Length=222, Percent_Identity=25.6756756756757, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787055, Length=246, Percent_Identity=23.5772357723577, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 29034; Mature: 29034

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEADLERAFEAGEFVMDYQPIVSIRSGAVTGAEATIRWDHPEWGALPQRVVHAAADRLG
CCHHHHHHHHHHCCHHCCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
AASRIAGHAFGEACRQLARWKQQGAGVSALSMRLSGAQLGAESVFERLALSIEAFDISSS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCHHHHHHHHHHHHHEEECCCC
QFTLEVPEMSAPEESLSLLDRLKRLRQKGYGIVLGDFGAHHTAMSTLMVLPVTGVKFGES
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHH
FTERLPGSPTAEAILSSVSRLAHDLGFTLTVSGVENGRQFELLRRFRDIELQGAFLFEPM
HHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHEECHH
RAEVWHERVNPRALSQLNLREPPF
HHHHHHHHCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MDEADLERAFEAGEFVMDYQPIVSIRSGAVTGAEATIRWDHPEWGALPQRVVHAAADRLG
CCHHHHHHHHHHCCHHCCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
AASRIAGHAFGEACRQLARWKQQGAGVSALSMRLSGAQLGAESVFERLALSIEAFDISSS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCHHHHHHHHHHHHHEEECCCC
QFTLEVPEMSAPEESLSLLDRLKRLRQKGYGIVLGDFGAHHTAMSTLMVLPVTGVKFGES
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHH
FTERLPGSPTAEAILSSVSRLAHDLGFTLTVSGVENGRQFELLRRFRDIELQGAFLFEPM
HHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHEECHH
RAEVWHERVNPRALSQLNLREPPF
HHHHHHHHCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]