Click here to switch to the map view.

The map label for this gene is rep [H]

Identifier: 126451499

GI number: 126451499

Start: 3891166

End: 3893253

Strand: Reverse

Name: rep [H]

Synonym: BURPS1106A_3991

Alternate gene names: 126451499

Gene position: 3893253-3891166 (Counterclockwise)

Preceding gene: 126453418

Following gene: 126453740

Centisome position: 97.61

GC content: 65.18

Gene sequence:

>2088_bases
ATGTCCGCAGGCCTGAATTCCGCTCAAAGCGAAGCGGTGCGCTATCTCGACGGTCCCTGCCTCGTGCTCGCCGGCGCGGG
CAGCGGCAAGACCCGCGTCATCACGCAGAAGATCGCGCACCTGATCGAGGCAAAAGGCTTCGAGCCGCGCCACATCGCCG
CCGTCACGTTCACGAACAAGGCGGCCGCCGAAATGCGCGAGCGCGTGGGCAAGCTCCTCGAGGGCAAGACGCTCACGACG
CCCGGCAAGGAAGGCCGCAAGGTGCCCGTCAATCAGCTGACGGTGTGCACGTTCCATTCGCTCGGCGTGCAGATTTTGCG
GCAGGAAGCCGAGCACGTCGGCCTGAAGCCGCAGTTCTCGATCATGGATTCGGACGACTGCTTCGGGATGATCCAGGAGC
AGATCGGCACGACCGACAAGGGCCTGATCCGCAAGATCCAGACGATCATTTCGCTGTGGAAGAACGGCCTCGTCGCGCCC
GAGGAGGCGATGACGATCGCCGCGAACGAGGACGAGCACCAGGCGGCGCTCGTCTACCGGAACTACGTCGCGACGCTGCA
CGCGTATCAGGCGGTCGACTTCGACGACCTGATCCGCCTGCCGACCGAACTGTTCGCGAGAAACGAGCCGGTGCGCGACC
GCTGGCAGAACAGGCTGCGCTATCTGCTGATCGACGAATATCAGGACACCAATGCGTGCCAGTACGAGCTGCTGAAGCTG
CTCGCGGGGCCGCGCGCGGCGTTCACGGCCGTGGGCGACGACGATCAGGCGATCTACGGCTGGCGCGGCGCGACGCTCGA
GAATCTCGCGCAGCTCGGCAAGGACTTCCCGAAACTGCACGTGATCAAGCTCGAGCAGAATTACCGGTCGACGGTGCGCA
TTCTGACCGCCGCGAACAACGTGATCGCGAACAACCCGAAGCTGTTCGAGAAGAAGCTGTGGTCCGAACACGGCATGGGC
GATTCGATCACCGTCACGCCGTGCAACGACGAAGAGCACGAGGCCGAATCCGTCGTGTTTCGCTTGTCCGCGCACAAGTT
CGAGCGGCGCACGCAGTTTCGCGACTACGCGATCCTGTACCGCGGCAACTTCCAGGCGCGCGTCTTCGAGCAGGTGCTGC
GGCGCGAGCGGATTCCCTACGTGCTGTCGGGCGGCCAGTCGTTCTTCGACAAGGCCGAGATCAAGGATTTGTGCGCGTAC
TTGCGGTTGATCGCGAACGCCGACGACGATCCCGCGTTCATCCGCGCGGTCACCACGCCGCGCCGCGGGATCGGCAACAC
GACGCTCGAGGCGCTCGGCGCGTTCGCGGGGCAGGCGAAGGTGTCGCTGTTCGAGGCGGTCTACATGGGCGGGATCGAAG
CGCGGCTGTCCGCGCGGCAGGTCGAGCCGCTGCGGATCTTCTGCGACTTCATCCAGCGCCTGACCGATCGCGCGGACAAG
GAGCCCGCGACCGTCGTCCTCGACGACATGATGGAGGCGATCCACTACGAAGCGTACCTGTACGACGCATACGACGAACG
GCAAGCGCAGACGAAGTGGCAGAACGTGCTCGAATTCCTTGAATGGCTGAAGCGCAAGGGAACGAAGCCGGAGGCCGAGC
AGGCGGTGGACGGCGAGGCCGAAGGGTTCCACAACGCGGACGGCCTCGCCGATACCGGCAAGAACCTGCTCGGGCTGATC
CAGACGGTCGCGCTGATGTCGATGCTCGAGGGCAAGGAAGAGGATCCGGACGCGGTGCGGCTGTCGACCGTGCATGCGTC
GAAGGGGCTCGAGTATCCGCACGTGTTCCTGGTTGGCGTCGAGGAAGGGATCATGCCGCACAGCGGCGGCAGCGACGACG
GCCCGATCGACGACGAGCGGATCGAGGAAGAGCGCCGGCTGATGTACGTCGCGATCACGCGCGCGCAGCGCAGCCTGCAT
CTGAACTGGTGCAAGAAGCGCAAGCGGGCGCGCGAGACGATCGTCTGCGAACAGTCGCGCTTCATCGCGGAGATGGGGCT
CGACGAGGCGCCGCCGCCTACGCCGGAAGAGGCGCCGATGACGCCGAAGGATCGCCTCGCGAGCCTGAAGGCGCTGCTGC
AGAAGTGA

Upstream 100 bases:

>100_bases
GTCCCGCGAGCCGTTCGCCGCCGCATACCACGCGCCGGCCGCACGTCGCTCGCAGCGCCTTCCGGCTTCGCCGCCGTCAA
CCGCAACACCATCCGCCATC

Downstream 100 bases:

>100_bases
CGCGGGCCGGGGGCCGCGCGATCGCGCCGGCCGCCGCCGGCCGGCTCGGCCCATTGCGCTCGGCCGGCGTTGCGGTTGGA
TCTTGCCGCGCGGCGGCATG

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MSAGLNSAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVGKLLEGKTLTT
PGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQIGTTDKGLIRKIQTIISLWKNGLVAP
EEAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPTELFARNEPVRDRWQNRLRYLLIDEYQDTNACQYELLKL
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMG
DSITVTPCNDEEHEAESVVFRLSAHKFERRTQFRDYAILYRGNFQARVFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAY
LRLIANADDDPAFIRAVTTPRRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEARLSARQVEPLRIFCDFIQRLTDRADK
EPATVVLDDMMEAIHYEAYLYDAYDERQAQTKWQNVLEFLEWLKRKGTKPEAEQAVDGEAEGFHNADGLADTGKNLLGLI
QTVALMSMLEGKEEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHSGGSDDGPIDDERIEEERRLMYVAITRAQRSLH
LNWCKKRKRARETIVCEQSRFIAEMGLDEAPPPTPEEAPMTPKDRLASLKALLQK

Sequences:

>Translated_695_residues
MSAGLNSAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVGKLLEGKTLTT
PGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQIGTTDKGLIRKIQTIISLWKNGLVAP
EEAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPTELFARNEPVRDRWQNRLRYLLIDEYQDTNACQYELLKL
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMG
DSITVTPCNDEEHEAESVVFRLSAHKFERRTQFRDYAILYRGNFQARVFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAY
LRLIANADDDPAFIRAVTTPRRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEARLSARQVEPLRIFCDFIQRLTDRADK
EPATVVLDDMMEAIHYEAYLYDAYDERQAQTKWQNVLEFLEWLKRKGTKPEAEQAVDGEAEGFHNADGLADTGKNLLGLI
QTVALMSMLEGKEEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHSGGSDDGPIDDERIEEERRLMYVAITRAQRSLH
LNWCKKRKRARETIVCEQSRFIAEMGLDEAPPPTPEEAPMTPKDRLASLKALLQK
>Mature_694_residues
SAGLNSAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVGKLLEGKTLTTP
GKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQIGTTDKGLIRKIQTIISLWKNGLVAPE
EAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPTELFARNEPVRDRWQNRLRYLLIDEYQDTNACQYELLKLL
AGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGD
SITVTPCNDEEHEAESVVFRLSAHKFERRTQFRDYAILYRGNFQARVFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAYL
RLIANADDDPAFIRAVTTPRRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEARLSARQVEPLRIFCDFIQRLTDRADKE
PATVVLDDMMEAIHYEAYLYDAYDERQAQTKWQNVLEFLEWLKRKGTKPEAEQAVDGEAEGFHNADGLADTGKNLLGLIQ
TVALMSMLEGKEEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHSGGSDDGPIDDERIEEERRLMYVAITRAQRSLHL
NWCKKRKRARETIVCEQSRFIAEMGLDEAPPPTPEEAPMTPKDRLASLKALLQK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=701, Percent_Identity=46.9329529243937, Blast_Score=583, Evalue=1e-167,
Organism=Escherichia coli, GI2367296, Length=669, Percent_Identity=36.0239162929746, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6322369, Length=730, Percent_Identity=28.4931506849315, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6324477, Length=667, Percent_Identity=23.5382308845577, Blast_Score=107, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78084; Mature: 77953

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGLNSAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNK
CCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
AAAEMRERVGKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFS
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQIGTTDKGLIRKIQTIISLWKNGLVAPEEAMTIAANEDEHQAALVYR
ECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
NYVATLHAYQAVDFDDLIRLPTELFARNEPVRDRWQNRLRYLLIDEYQDTNACQYELLKL
HHHHHHHHHHCCCHHHHHHCCHHHHHCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHH
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANN
HCCCCCEEEEECCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEECCCCHHHEEEHHHCCC
VIANNPKLFEKKLWSEHGMGDSITVTPCNDEEHEAESVVFRLSAHKFERRTQFRDYAILY
HHCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNFQARVFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAFIRAVTTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
RRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEARLSARQVEPLRIFCDFIQRLTDRADK
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
EPATVVLDDMMEAIHYEAYLYDAYDERQAQTKWQNVLEFLEWLKRKGTKPEAEQAVDGEA
CCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCC
EGFHNADGLADTGKNLLGLIQTVALMSMLEGKEEDPDAVRLSTVHASKGLEYPHVFLVGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEE
EEGIMPHSGGSDDGPIDDERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETIVCEQSR
CCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAEMGLDEAPPPTPEEAPMTPKDRLASLKALLQK
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAGLNSAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNK
CCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
AAAEMRERVGKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFS
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQIGTTDKGLIRKIQTIISLWKNGLVAPEEAMTIAANEDEHQAALVYR
ECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
NYVATLHAYQAVDFDDLIRLPTELFARNEPVRDRWQNRLRYLLIDEYQDTNACQYELLKL
HHHHHHHHHHCCCHHHHHHCCHHHHHCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHH
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANN
HCCCCCEEEEECCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEECCCCHHHEEEHHHCCC
VIANNPKLFEKKLWSEHGMGDSITVTPCNDEEHEAESVVFRLSAHKFERRTQFRDYAILY
HHCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNFQARVFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAFIRAVTTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
RRGIGNTTLEALGAFAGQAKVSLFEAVYMGGIEARLSARQVEPLRIFCDFIQRLTDRADK
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
EPATVVLDDMMEAIHYEAYLYDAYDERQAQTKWQNVLEFLEWLKRKGTKPEAEQAVDGEA
CCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCC
EGFHNADGLADTGKNLLGLIQTVALMSMLEGKEEDPDAVRLSTVHASKGLEYPHVFLVGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEE
EEGIMPHSGGSDDGPIDDERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETIVCEQSR
CCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAEMGLDEAPPPTPEEAPMTPKDRLASLKALLQK
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]