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The map label for this gene is metK [H]

Identifier: 126451461

GI number: 126451461

Start: 199179

End: 200366

Strand: Direct

Name: metK [H]

Synonym: BURPS1106A_0211

Alternate gene names: 126451461

Gene position: 199179-200366 (Clockwise)

Preceding gene: 126452974

Following gene: 126454571

Centisome position: 4.99

GC content: 67.09

Gene sequence:

>1188_bases
GTGGCAAACGATTATCTCTTCACGTCCGAATCCGTTTCCGAAGGCCATCCGGACAAGGTCGCCGACCAAATCTCCGACGC
GATCCTCGACGCAATCCTCGCGCAAGACAAGTACTCCCGTGTCGCGGCCGAAACGCTGTGCAACACCGGTCTCGTCGTGC
TCGCCGGCGAAATCACGACCACGGCGAACATCGACTACATCCAGATCGCGCGCGACACGATCAAGCGCATCGGCTACGAC
AATACCGATTACGGCATCGACTACCGCGGCTGCGCGGTGCTCGTCGCGTACGACAAGCAGTCGCCGGACATCGCGCAAGG
CGTCGACCGCGCGCACGACAACAACCTCGATCAGGGCGCGGGCGATCAGGGCCTGATGTTCGGCTACGCGTGCGACGAAA
CGCCCGAGCTGATGCCGCTGCCGATCCACCTGTCGCACCGGCTCGTCGAGCGCCAGGCGAACCTGCGCCGCGACGGCCGC
CTGCCGTGGCTGCGCCCGGACGCGAAGTCACAGGTGACGGTGCGCTACGTCGACGGCAAGCCGCACTCGATCGACACCGT
CGTGCTATCGACGCAGCACGCGCCGGAGATCGATCTGCCCGCGCTGCGCGAAGCGGTGATCGAAGAGGTCATCAAGCCGA
CGCTGCCCGCCGACCTGATCAAGGGCGACATCAAGTTCCTCGTGAACCCGACCGGCCGCTTCGTGATCGGCGGCCCGCAA
GGCGATTGCGGGCTGACCGGCCGCAAGATCATCGTCGACACCTACGGCGGCGCGGCGCCGCACGGCGGCGGCGCGTTCTC
GGGCAAGGATCCGTCGAAGGTCGACCGCTCGGCCGCCTACGCGGGCCGCTACGTCGCGAAGAACATCGTCGCCGCGGGCC
TCGCGTCGCGCGCGCTGATTCAGGTGTCGTACGCGATCGGCGTGGCCGAGCCGACCTCGGTGATGGTCAACACGTTCGGC
ACGGGCCGCGTGTCCGACGAGACGATCACGAAGCTCGTGCGCGAGCACTTCGACCTGCGTCCGAAGGGCATCATCCAGAT
GCTCGACCTGCTGCGCCCGATCTACGAGAAGACCGCCGCTTACGGCCACTTCGGCCGCGAAGAACCGGAGTTCTCGTGGG
AAGCCGCCGACAAGGCGCTCGCGCTCGCCGAAGCGGCGGGCGTCGAGCCGGCCGTGCAAGTGGCCTGA

Upstream 100 bases:

>100_bases
GCACCCACGGCGCAGGAAATCCGCTAAAGCGTCGCCACTTCGGGCCTCTGGAAGCTGGCTACGTGAACTCAACCGTAACC
AAAAACAGGAGCCTGAGAAC

Downstream 100 bases:

>100_bases
CGCACGCGCGTGCTCGCGCGCAAACAAAAACCCCGGCTTCGGCCGGGGTTTTTTCATTTCAATGGTGCGTCGGATGCGGC
TCGCATGGCGCATCGGCGGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYD
NTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGR
LPWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHAPEIDLPALREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA

Sequences:

>Translated_395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYD
NTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGR
LPWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHAPEIDLPALREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA
>Mature_394_residues
ANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIKRIGYDN
TDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRL
PWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHAPEIDLPALREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGT
GRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=58.6842105263158, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=57.3684210526316, Blast_Score=422, Evalue=1e-118,
Organism=Escherichia coli, GI1789311, Length=385, Percent_Identity=69.8701298701299, Blast_Score=539, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=58.7765957446808, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=58.421052631579, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=58.2446808510638, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=55.7840616966581, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=57.3170731707317, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=52.9255319148936, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=56.6265060240964, Blast_Score=355, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=57.1428571428571, Blast_Score=319, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=58.3333333333333, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=58.5751978891821, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=59.2592592592593, Blast_Score=431, Evalue=1e-122,
Organism=Drosophila melanogaster, GI45552159, Length=378, Percent_Identity=54.7619047619048, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580529, Length=378, Percent_Identity=54.7619047619048, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580537, Length=378, Percent_Identity=54.7619047619048, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580533, Length=378, Percent_Identity=54.7619047619048, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580535, Length=378, Percent_Identity=54.7619047619048, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580531, Length=378, Percent_Identity=54.7619047619048, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580527, Length=376, Percent_Identity=54.2553191489362, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=376, Percent_Identity=54.2553191489362, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=51.9893899204244, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=53.781512605042, Blast_Score=132, Evalue=6e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42642; Mature: 42510

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIKRIGYDNTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRLPWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSIDTVVLSTQHAPEIDLPALREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure 
ANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIKRIGYDNTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRLPWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSIDTVVLSTQHAPEIDLPALREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEAADKALALAEAAGVEPAVQVA
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA