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The map label for this gene is flhA [H]

Identifier: 126451444

GI number: 126451444

Start: 849919

End: 851025

Strand: Direct

Name: flhA [H]

Synonym: BURPS1106A_0866

Alternate gene names: 126451444

Gene position: 849919-851025 (Clockwise)

Preceding gene: 126454129

Following gene: 126453351

Centisome position: 21.31

GC content: 64.95

Gene sequence:

>1107_bases
ATGAAGACGAAAGCCGCGATCGCCTGGAAGGCGGGCGAACCGCTGACGATCGAAGAGGTCGATCTCGAAGGACCGCGCGC
AGGCGAGGTCCTGATCGAAGTGAAGGCCACCGGCATCTGCCACACCGATTATTACACCCTCTCGGGCGCGGACCCCGAGG
GCATCTTCCCGGCGATCCTCGGGCACGAGGGCGCGGGCGTCGTCGTCGACGTCGGGCCGGGCGTCGGCACGCTGAAGAAG
GGCGATCACGTGATTCCGCTCTACACGCCCGAATGCCGCGAGTGCAAGTTCTGCCTGTCGCGCAAGACGAACCTGTGCCA
GAAGATCCGCGCGACCCAAGGCCGCGGCCTGATGCCCGACGCGACGTCGCGCTTCTCGGTCGGCGGCAAGCCGATCTTCC
ACTACATGGGCACGTCCACGTTCTCGAACTACATCGTGGTGCCGGAGATCGCGGTCGCGAAGGTGCGCGAGGACGCGCCG
TTCGACAAGATCTGCTACATCGGCTGCGGCGTGACGACGGGCGTCGGCGCGGTCGTCTACTCGGCGAAGGTCGAGGCGGG
CGCGAACGTCGTCGTGTTCGGCCTCGGCGGCATCGGCCTGAATGTGATCCAGGGCGCGAGGATGGTGGGCGCGGACAAGA
TCATCGGCGTCGATCTCAACCCGAAGCGCGTCGAGCTCGCGAGGAAGTTCGGCATGACGCACTTCATCAACCCGAACGAA
GTCGAGAACGTCGTCGACCACATCGTGCAGTTGACGGACGGCGGCGCCGATTATTCGTTCGAATGCATCGGCAACGTGAA
GGTGATGCGCCAGGCGCTCGAGTGCACGCACAAGGGGTGGGGGCAGTCGTTCATCATCGGCGTCGCGGCGGCCGGCGAGG
AAATCAGCACGCGGCCGTTCCAGCTCGTGACCGGCCGCGAGTGGAAGGGCTCCGCGTTCGGCGGCGCGCGCGGGCGCACC
GACGTGCCGAAGATCGTCGATTGGTACATGGAAGGCAAGATCAACATCGACGACCTGATCACGCACACGCTGCCGCTCGA
GAAGATCAACGAAGGCTTCGAGCTGATGAAGAAGGGCGAATCGATCCGCTCGGTCGTGCTGTACTGA

Upstream 100 bases:

>100_bases
CGCCGATTCCCGCCAATTTCCGGCCCGCGGCCGCCGTGCCGGCCGCGCATGCGGATACACTTGACGGCATTGCACCCAGT
GACTTTTCCGGAGAACACGC

Downstream 100 bases:

>100_bases
TCGGGAGCCTTGCATGGAAACGACGACGCTCGAATGCGTTTCTTCGCATGCGTGCCACGGCGGCGAGCAGCGCTTCTATC
GGCATGAATCGAAAACGATC

Product: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTLKK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPDATSRFSVGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDLNPKRVELARKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHTLPLEKINEGFELMKKGESIRSVVLY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTLKK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPDATSRFSVGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDLNPKRVELARKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHTLPLEKINEGFELMKKGESIRSVVLY
>Mature_368_residues
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTLKK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPDATSRFSVGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDLNPKRVELARKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHTLPLEKINEGFELMKKGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=61.3079019073569, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=46.524064171123, Blast_Score=363, Evalue=1e-101,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=46.070460704607, Blast_Score=351, Evalue=6e-97,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.7994579945799, Blast_Score=347, Evalue=8e-96,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=44.9864498644986, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=44.9864498644986, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.2574525745258, Blast_Score=340, Evalue=9e-94,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=44.5652173913043, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=44.5983379501385, Blast_Score=327, Evalue=8e-90,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=59.0785907859079, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI87082125, Length=379, Percent_Identity=26.9129287598945, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786825, Length=401, Percent_Identity=25.1870324189526, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=26.8698060941828, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790045, Length=376, Percent_Identity=26.5957446808511, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI226510992, Length=372, Percent_Identity=24.7311827956989, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=25.3602305475504, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081918, Length=360, Percent_Identity=28.6111111111111, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788075, Length=382, Percent_Identity=27.2251308900524, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790718, Length=328, Percent_Identity=24.0853658536585, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=24.7956403269755, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.1554959785523, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=53.8666666666667, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17565904, Length=337, Percent_Identity=55.1928783382789, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=28.6103542234332, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988145, Length=371, Percent_Identity=26.1455525606469, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=363, Percent_Identity=25.068870523416, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17562878, Length=360, Percent_Identity=25.2777777777778, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=53.6388140161725, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323099, Length=371, Percent_Identity=29.1105121293801, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=373, Percent_Identity=27.6139410187668, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=364, Percent_Identity=27.7472527472527, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319621, Length=372, Percent_Identity=27.4193548387097, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=26.6483516483516, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322619, Length=363, Percent_Identity=29.2011019283747, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319955, Length=363, Percent_Identity=28.6501377410468, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319257, Length=367, Percent_Identity=26.158038147139, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=256, Percent_Identity=28.515625, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=59.5108695652174, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17137530, Length=357, Percent_Identity=27.4509803921569, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39634; Mature: 39634

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCCEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVGTLKKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSVGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCCCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDLNPKRVELARKFGMTHFINPNE
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHTLPLEKINEGFELMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCC
SIRSVVLY
CHHHEECC
>Mature Secondary Structure
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCCEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVGTLKKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSVGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCCCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDLNPKRVELARKFGMTHFINPNE
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHTLPLEKINEGFELMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCC
SIRSVVLY
CHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]