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The map label for this gene is paaF [C]

Identifier: 126447762

GI number: 126447762

Start: 1739567

End: 1740394

Strand: Reverse

Name: paaF [C]

Synonym: BMA10247_A1802

Alternate gene names: 126447762

Gene position: 1740394-1739567 (Counterclockwise)

Preceding gene: 126446563

Following gene: 126445641

Centisome position: 73.97

GC content: 65.94

Gene sequence:

>828_bases
ATGAGTTATGAAGGTCGCTGGAAAACCGTCGAGGTGATCGTCGACGGCGCAATCGCATGGGTGACGCTGAATCGTCCCGA
CAAGCGCAACGCGATGAGCCCGACGCTGAACGCGGAGATGATCGACGTGCTGGAGGCGATCGAGCTCGATCCCGAAGCGC
GGGTGCTCGTGCTGACGGGCGAGGGCGAGGCGTGGACGGCCGGCATGGACCTGAAGGAGTACTTTCGCGAAATCGACGCC
GGCCCCGAGATCCTGCAGGAGAAGATCCGCCGCGACGCGAGCCGCTGGCAGTGGCAGTTGCTGCGCATGTATGCGAAGCC
GACGATCGCGATGGTCAACGGCTGGTGCTTCGGCGGCGGGTTCTCGCCGCTCGTCGCGTGCGATCTCGCGATCGCGGCCG
ACGAGGCCGTGTTCGGCCTGTCGGAGATCAACTGGGGCATTCCGCCCGGCAATCTCGTCAGCAAGGCGATGGCCGATACG
GTCGGCCATCGTCAGGCGCTGTACTACATCATGACGGGCGAGACCTTCACGGGCGCGCAGGCCGCGCAGATGGGGCTCGT
CAACCGGAGCGTGCCGCGCGCGCAACTGCGCGACGCGGTGCGCGCGCTCGCCGCGAAGCTGCTCGACAAGAATCCGGTCG
TGCTGCGCAACGCGAAGCACGGCTTCAAGCGCTGCCGCGAGCTCACGTGGGAGCAGAACGAGGACTACCTGTACGCGAAG
CTCGATCAGGCGCAGTTGCGCGATCCCGAGCACGGGCGCGAGCAGGGCCTCAAGCAATTCCTCGACGACAAGACGATCAA
GCCGGGCTTGCAGGCGTACAGGCGTTGA

Upstream 100 bases:

>100_bases
TTTGTCCGGACCTCCGCCGATACTGCTTTACATATCAGGTAACCTGATATTAAATCAGCAGTGAGCCGCGCGTCGCGGCA
ATGTCCCTGGAGAAAGATCA

Downstream 100 bases:

>100_bases
GCGCGCGCCCCGGGTGCGCCGGGCCGCAACGCGATGAAGCGACGGCGCGATGAAGCGGCATGCGGCGGCGGCATGCCGCA
GGCGGCGCGCGGCGCGGCAA

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSYEGRWKTVEVIVDGAIAWVTLNRPDKRNAMSPTLNAEMIDVLEAIELDPEARVLVLTGEGEAWTAGMDLKEYFREIDA
GPEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGGFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADT
VGHRQALYYIMTGETFTGAQAAQMGLVNRSVPRAQLRDAVRALAAKLLDKNPVVLRNAKHGFKRCRELTWEQNEDYLYAK
LDQAQLRDPEHGREQGLKQFLDDKTIKPGLQAYRR

Sequences:

>Translated_275_residues
MSYEGRWKTVEVIVDGAIAWVTLNRPDKRNAMSPTLNAEMIDVLEAIELDPEARVLVLTGEGEAWTAGMDLKEYFREIDA
GPEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGGFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADT
VGHRQALYYIMTGETFTGAQAAQMGLVNRSVPRAQLRDAVRALAAKLLDKNPVVLRNAKHGFKRCRELTWEQNEDYLYAK
LDQAQLRDPEHGREQGLKQFLDDKTIKPGLQAYRR
>Mature_274_residues
SYEGRWKTVEVIVDGAIAWVTLNRPDKRNAMSPTLNAEMIDVLEAIELDPEARVLVLTGEGEAWTAGMDLKEYFREIDAG
PEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGGFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADTV
GHRQALYYIMTGETFTGAQAAQMGLVNRSVPRAQLRDAVRALAAKLLDKNPVVLRNAKHGFKRCRELTWEQNEDYLYAKL
DQAQLRDPEHGREQGLKQFLDDKTIKPGLQAYRR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=28.740157480315, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI70995211, Length=234, Percent_Identity=29.4871794871795, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=29.2307692307692, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI45643119, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI260275230, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=229, Percent_Identity=27.5109170305677, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=25.2631578947368, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=31.0756972111554, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI221142681, Length=238, Percent_Identity=31.5126050420168, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788597, Length=215, Percent_Identity=35.3488372093023, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=23.0483271375465, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=26.9607843137255, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790281, Length=203, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787660, Length=198, Percent_Identity=28.7878787878788, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=223, Percent_Identity=30.0448430493274, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=27.2, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=28.8461538461538, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=227, Percent_Identity=27.7533039647577, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=234, Percent_Identity=24.3589743589744, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=203, Percent_Identity=28.0788177339901, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=25.2747252747253, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129971, Length=273, Percent_Identity=26.7399267399267, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24653477, Length=273, Percent_Identity=26.7399267399267, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650670, Length=184, Percent_Identity=31.5217391304348, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=30.5970149253731, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21357171, Length=185, Percent_Identity=29.1891891891892, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920382, Length=246, Percent_Identity=26.0162601626016, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922422, Length=240, Percent_Identity=24.5833333333333, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30951; Mature: 30820

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEGRWKTVEVIVDGAIAWVTLNRPDKRNAMSPTLNAEMIDVLEAIELDPEARVLVLTG
CCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
EGEAWTAGMDLKEYFREIDAGPEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGG
CCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECCC
FSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADTVGHRQALYYIMTGETFTGAQ
CHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
AAQMGLVNRSVPRAQLRDAVRALAAKLLDKNPVVLRNAKHGFKRCRELTWEQNEDYLYAK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCEEEEE
LDQAQLRDPEHGREQGLKQFLDDKTIKPGLQAYRR
ECHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHCC
>Mature Secondary Structure 
SYEGRWKTVEVIVDGAIAWVTLNRPDKRNAMSPTLNAEMIDVLEAIELDPEARVLVLTG
CCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
EGEAWTAGMDLKEYFREIDAGPEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGG
CCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECCC
FSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADTVGHRQALYYIMTGETFTGAQ
CHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
AAQMGLVNRSVPRAQLRDAVRALAAKLLDKNPVVLRNAKHGFKRCRELTWEQNEDYLYAK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCEEEEE
LDQAQLRDPEHGREQGLKQFLDDKTIKPGLQAYRR
ECHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA