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The map label for this gene is rhf [H]

Identifier: 126447706

GI number: 126447706

Start: 535236

End: 537590

Strand: Direct

Name: rhf [H]

Synonym: BMA10247_A0588

Alternate gene names: 126447706

Gene position: 535236-537590 (Clockwise)

Preceding gene: 126447323

Following gene: 126446407

Centisome position: 22.75

GC content: 69.98

Gene sequence:

>2355_bases
ATGACGACGCATTCCGCGTCCACGCCGCCGGCCTTGCCCGCCGCGCAGTGTCCGTTCCGCCCCGTTTCAGACGATGCCGC
CGCGCATCCGGCCGGTTGCCCGGCGAGCGCCGGGGCGGCGGCATTCGATCCGTTCGACGACGCCTATCAACAGGACCCGC
CCGAGTACGTGCGTTGGGCGCGCGAGCGCGAGCCGGTGTTCTACAGCCCGAAGCTCGGTTACTGGGTCGTCGCGCGCTAT
GACGACATCAAGACGATCTTTCGCGACAACCGGACCTTCAGCCCGTCGATCGCGCTCGAGAAGATCACGCCGACGGGGCC
GGACGCGAACGCGGCGCTCGCGTCGTACGGCTATGCGATGGGCCGCACGCTCGTCAACGAGGACGAGCCCACGCACATGG
CGCGGCGGCGTCTGCTGATCGAGCCGTTCACGCCCGACGCGCTGCGCGCGCATGAGCCGATGGTGCGACGGCTTGCGCGC
GAATACGTCGACCGCTTCGTCGACGATGGCCGCGCGGACCTCGTCGACCAGATGCTGTGGGAAGTGCCGCTCACGGTCGC
GCTGCATTTTCTGGGCGTGCCCGAAGCGGACATGGATACGCTGCGCGCGTACTCGATCGCGCATACCGTCAACACCTGGG
GACGGCCGCGGCCGGACGAGCAAGTTGCGGTCGCGCATGCGGTCGGACAGTTCTGGCAGTACGCGGGCAAGGTGCTCGAG
CAGATGCGGGAGGATCCGTCGGGGCCCGGCTGGATGCAGTTCGGCATCCGCATGCAGCGCGAGCATCCGGACATCGTCAC
CGATTCGTATCTGCATTCGATGATGATGGCGGGCATCGTTGCCGCGCACGAGACCACCGCGAACGCAACGGCGAACGCGC
TGAAGCTGTTGCTCGAGCATCCGCGCGCGTGGCGAGAGCTGTGTGACGACCCTGCGCTGATCCCGAACGCGGTCGACGAG
TGCCTGCGTCACAGCGGATCGATCGTCGCGTGGCGGCGGATCGCGACGCGCGACGCGACGGTCGGCGGCGTTGCGATTCC
CGCCGGCGCGAAGCTGTTGATCGTCACGTCGTCGGCGAATCGTGACGAGCGCCGCTTCGCCGATCCGGACCTGTTCGACA
TCCACCGCGACAATCCCGCCGATCACCTGACGTTCGGCTACGGTTCGCATCAGTGCATGGGCAAGCATCTCGCGCGCATG
GAGATGCAGATCTTCATCGACGAGCTGTCGCGCCGCCTGCCGCACATGCGGCTCGCCGAACAGCGCTTCACGTATGTGCC
GAACACGTCGTTTCGTGGTCCGGAGCATCTGTGGGTCGAATGGGAGCCGGCGGAGAATCCGCAGCGGCGCGGCCACGGCG
CGCGCCGCTGCGCGCTTGCCGTGCGCATCGGCGAATCGAACGCGCGCCGCGCGGGGCGCGCGCTGCGCGTCGACAGCGTG
ATGCCGGTGGCCGAGCGCGTGCTGCGCGTACGCCTCGTCGCGCCCGACGGCGCGCCGCTGCCGCGCTGGAGCGCGGGCGC
GCATATCGACGTTGAATGCGGCGACACCGGGCTCTCCCGACAGTACTCGCTGTGCGGCGATCCGGCCGAGCGCGGCGTAT
ACGAGATCGCGGTGCTGCGCGAGCCGGCGAGCCGCGGCGGCTCCGCATGGATGCACGAGCGGCTGCGGCCCGGCATGCGC
GTGAAGATTCGCGGGCCGCGCAACCACTTCCGGCTCGACGGGCACGCCGCCCGCGCGATCCTGATCGCGGGCGGCATCGG
CATCACGCCGATCGGCGCGATCGCACGCGAGGCGCGCGCGCGCGGCATCGACTACGCGCTGCATTACTGCGGCCGCAGCC
GCCGGACGATGGCGTTCGTCGACGAGCTCGCCGCGCTGCACGGTGAGCGGCTTCGCCTGCACGTGTCGGACGAAGGCACG
CGGCATGATTTTTCCGCGCTCGACGTCGCTGCCGGCACGCAGGTGTATGCGTGCGGTCCCGCGCGCATGCTCGACGCGCT
GGCCGCGTGCGCCGCGCATTGGCCGGCGCATGCGCTGCGCGTCGAGCATTTCGCGGCCGCCGCGCCGCGCGTCGATCCGC
GCACCGATCAGCCGTTCGAGGTCGAGCTGAAGGATTCGGGGCTCACGCTGACGGTGCCCGCGAACCGGACGCTGCTGGCG
ACGCTGCGCGCGGCGAACGTCGACGTGCAGAGCGACTGCGAGGAAGGGCTGTGCGGCTCGTGCGAGGTGCGCGTGCTTGC
CGGCGAGGTTGACCATCGTGACAGCGTGCTGAGCGCGGACGAGCGTGCGCTGAACACGCGCATGATGGCGTGCTGCTCGC
GCGCGAGGCACGGACGGCTCGTGCTCGAACTGTAG

Upstream 100 bases:

>100_bases
GGCGATGCAGGTTAACGCGATCATTGATTTTGCGCAGTGCGTACTAGCGTTACGCAAAGCGCAATTCGATCCAGCGTAAT
TCGACCCCTGGAGGACTGCC

Downstream 100 bases:

>100_bases
CTGTAGCGTGAGGCCCGTCGCCCCGGCGCGGGACGGACGAAGACAACGGCATGGCGGGAGGCGACGAATGACGGCGCGAG
TGGACGTGCGCGCGAGGCTC

Product: cytochrome P450

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 784; Mature: 783

Protein sequence:

>784_residues
MTTHSASTPPALPAAQCPFRPVSDDAAAHPAGCPASAGAAAFDPFDDAYQQDPPEYVRWAREREPVFYSPKLGYWVVARY
DDIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAMGRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLAR
EYVDRFVDDGRADLVDQMLWEVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLE
QMREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEHPRAWRELCDDPALIPNAVDE
CLRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSANRDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARM
EMQIFIDELSRRLPHMRLAEQRFTYVPNTSFRGPEHLWVEWEPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSV
MPVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLREPASRGGSAWMHERLRPGMR
VKIRGPRNHFRLDGHAARAILIAGGIGITPIGAIAREARARGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGT
RHDFSALDVAAGTQVYACGPARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLA
TLRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARHGRLVLEL

Sequences:

>Translated_784_residues
MTTHSASTPPALPAAQCPFRPVSDDAAAHPAGCPASAGAAAFDPFDDAYQQDPPEYVRWAREREPVFYSPKLGYWVVARY
DDIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAMGRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLAR
EYVDRFVDDGRADLVDQMLWEVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLE
QMREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEHPRAWRELCDDPALIPNAVDE
CLRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSANRDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARM
EMQIFIDELSRRLPHMRLAEQRFTYVPNTSFRGPEHLWVEWEPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSV
MPVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLREPASRGGSAWMHERLRPGMR
VKIRGPRNHFRLDGHAARAILIAGGIGITPIGAIAREARARGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGT
RHDFSALDVAAGTQVYACGPARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLA
TLRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARHGRLVLEL
>Mature_783_residues
TTHSASTPPALPAAQCPFRPVSDDAAAHPAGCPASAGAAAFDPFDDAYQQDPPEYVRWAREREPVFYSPKLGYWVVARYD
DIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAMGRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLARE
YVDRFVDDGRADLVDQMLWEVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLEQ
MREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEHPRAWRELCDDPALIPNAVDEC
LRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSANRDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARME
MQIFIDELSRRLPHMRLAEQRFTYVPNTSFRGPEHLWVEWEPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSVM
PVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLREPASRGGSAWMHERLRPGMRV
KIRGPRNHFRLDGHAARAILIAGGIGITPIGAIAREARARGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGTR
HDFSALDVAAGTQVYACGPARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLAT
LRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARHGRLVLEL

Specific function: Degradation of thiocarbamate herbicides [H]

COG id: COG2124

COG function: function code Q; Cytochrome P450

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome P450 family [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=320, Percent_Identity=40.3125, Blast_Score=220, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001128
- InterPro:   IPR002397
- InterPro:   IPR017972 [H]

Pfam domain/function: PF00067 p450 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 86814; Mature: 86683

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00086 CYTOCHROME_P450 ; PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHSASTPPALPAAQCPFRPVSDDAAAHPAGCPASAGAAAFDPFDDAYQQDPPEYVRWA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
REREPVFYSPKLGYWVVARYDDIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAM
HHCCCEEECCCCCEEEEEEEHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
GRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLAREYVDRFVDDGRADLVDQMLW
CCHHCCCCCHHHHHHHHHEECCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QMREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEH
HHHHCCCCCCCEEECEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
PRAWRELCDDPALIPNAVDECLRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSAN
CHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEECCCCEEEEEECCCC
RDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARMEMQIFIDELSRRLPHMRLAE
CCHHHCCCCCCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRFTYVPNTSFRGPEHLWVEWEPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSV
HHHEECCCCCCCCCCEEEEEECCCCCHHHHCCCHHEEEEEEEECCCCHHHCCCEEEECHH
MPVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLR
HHHHHHHHEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCEEEEEEEE
EPASRGGSAWMHERLRPGMRVKIRGPRNHFRLDGHAARAILIAGGIGITPIGAIAREARA
CCHHCCCHHHHHHHCCCCCEEEEECCCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHH
RGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGTRHDFSALDVAAGTQVYACGP
CCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCEEEECCH
ARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLA
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEE
TLRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARHGRL
EHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLEL
EEEC
>Mature Secondary Structure 
TTHSASTPPALPAAQCPFRPVSDDAAAHPAGCPASAGAAAFDPFDDAYQQDPPEYVRWA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
REREPVFYSPKLGYWVVARYDDIKTIFRDNRTFSPSIALEKITPTGPDANAALASYGYAM
HHCCCEEECCCCCEEEEEEEHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
GRTLVNEDEPTHMARRRLLIEPFTPDALRAHEPMVRRLAREYVDRFVDDGRADLVDQMLW
CCHHCCCCCHHHHHHHHHEECCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EVPLTVALHFLGVPEADMDTLRAYSIAHTVNTWGRPRPDEQVAVAHAVGQFWQYAGKVLE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QMREDPSGPGWMQFGIRMQREHPDIVTDSYLHSMMMAGIVAAHETTANATANALKLLLEH
HHHHCCCCCCCEEECEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
PRAWRELCDDPALIPNAVDECLRHSGSIVAWRRIATRDATVGGVAIPAGAKLLIVTSSAN
CHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEECCCCEEEEEECCCC
RDERRFADPDLFDIHRDNPADHLTFGYGSHQCMGKHLARMEMQIFIDELSRRLPHMRLAE
CCHHHCCCCCCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRFTYVPNTSFRGPEHLWVEWEPAENPQRRGHGARRCALAVRIGESNARRAGRALRVDSV
HHHEECCCCCCCCCCEEEEEECCCCCHHHHCCCHHEEEEEEEECCCCHHHCCCEEEECHH
MPVAERVLRVRLVAPDGAPLPRWSAGAHIDVECGDTGLSRQYSLCGDPAERGVYEIAVLR
HHHHHHHHEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCEEEEEEEE
EPASRGGSAWMHERLRPGMRVKIRGPRNHFRLDGHAARAILIAGGIGITPIGAIAREARA
CCHHCCCHHHHHHHCCCCCEEEEECCCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHH
RGIDYALHYCGRSRRTMAFVDELAALHGERLRLHVSDEGTRHDFSALDVAAGTQVYACGP
CCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCEEEECCH
ARMLDALAACAAHWPAHALRVEHFAAAAPRVDPRTDQPFEVELKDSGLTLTVPANRTLLA
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEE
TLRAANVDVQSDCEEGLCGSCEVRVLAGEVDHRDSVLSADERALNTRMMACCSRARHGRL
EHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLEL
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]