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The map label for this gene is phaZ1 [H]

Identifier: 126447333

GI number: 126447333

Start: 292132

End: 293226

Strand: Direct

Name: phaZ1 [H]

Synonym: BMA10247_A0332

Alternate gene names: 126447333

Gene position: 292132-293226 (Clockwise)

Preceding gene: 126446169

Following gene: 126447870

Centisome position: 12.42

GC content: 70.96

Gene sequence:

>1095_bases
ATGCCGAACGCGCGGACGTCGGCGTCCGCCCTGAAGGCTGCCCAAAAGATGACGGCCGACTGGCCGTTCGCCGAAACGAT
GGCCGCGGCGGCGGCCGCTTCAGTCGCCTCCGTCGCGGCGTCCGCCGACACGGTACCGCCCGCCGCGCGCGAATCGCGCG
TACGGCCGCGCGCGGCCGCTTGGGCGGGCGGCGAATGGATCCGCGCCGATCACCCGTTGCCGCCCGCGTTCGGGCGCTTC
GTCAAGCATCTCGAATACGGGCTGTACGTGCCGTCCGGCGCGAAGACGCTCGGTTTGCCGCTCGTCGTGATGCTGCACGG
CTGCAAGCAGGACATGAATCAGTTCTCGCAAGGCACGCGGATGAACTTGCTCGCGGACCGATACGGCTTCGCCGTGCTCT
ACCCGGAGCAGTCGCTCAGCGCGCACGCGCACGGCTGCTGGCACTGGTACGAGGACACCGTGCACGGCGGGCGCGGCGAA
GCGCAGGCGGTCGTCGCGCTCGTCGACGCGCTCGTCGCCGAGCGCGGCTTTGACGCATCGCGCGTGTATGCGGCCGGCAT
GTCGGCGGGCGCGGGGCTCGTATCGTTGCTCGCGCTGCATTTCCCGCGGCGCTTCGCGGCCGTGGCGCTCCATTCGGGGC
CGGCGTTCGGCGATGCGCATTCCGGGATCACCGCGATGGACGTGATGCGCCGCGGCCTGCACCGCGATCCCGCCGCCGTT
GTCGACGCGCTCGTCGAGCCGGGCGCGCATCCGGGAATGCCGGCCTTCATCGTGCACGGCGACGACGATCGCGTTGTCGT
GCCGAAGAACGCGGACGAACTCGCCGTTCAGTTCCTGCGGCTGAACGGTCTTGCCGACGCCGATGGTGAACCGGCGGGCG
TCGAGCGTTTCGAGACGCGCGCAGCCGACGTGCGCACGCTCGACTATCGCCGCGACGGCGAATCGGTCGTGCGCCTCTGC
CGGGTCCACGGCCTCGCGCATGCGTGGGCGGGCGGCGACGACAGTGTGCCGTTCCACTCGGCCGCCGGCCCGGATGCGAG
CGAGCTGATCTGGTCTTTCTTCGCGTCGCGTGAGCGAGCGGCTGTCGCGTCGTAA

Upstream 100 bases:

>100_bases
CACCCACTGAGGTGTCCCTCATTCTTCTAACTGCGAGCCCCGACCGATGACCAAAAGTCTGACGAAATTCTGGCTGGGCG
GTGTGAAACGCATGCTGCAC

Downstream 100 bases:

>100_bases
CGACGCACGCTCGGGAACGGCTGGCATTGATTCAGGGTTTTCCCTATAATACGGGTAAATACCTAAGAGAAAACCCTTGG
ATTGGAGTGACCGATCATGT

Product: polyhydroxybutyrate depolymerase domain-containing protein

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MPNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWAGGEWIRADHPLPPAFGRF
VKHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGE
AQAVVALVDALVAERGFDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAV
VDALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAADVRTLDYRRDGESVVRLC
RVHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERAAVAS

Sequences:

>Translated_364_residues
MPNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWAGGEWIRADHPLPPAFGRF
VKHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGE
AQAVVALVDALVAERGFDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAV
VDALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAADVRTLDYRRDGESVVRLC
RVHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERAAVAS
>Mature_363_residues
PNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWAGGEWIRADHPLPPAFGRFV
KHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGEA
QAVVALVDALVAERGFDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAVV
DALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAADVRTLDYRRDGESVVRLCR
VHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERAAVAS

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38627; Mature: 38496

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAA
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHH
WAGGEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTR
CCCCCEEECCCCCCHHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
MNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGEAQAVVALVDALVAERGFDAS
HHHHHHHCCEEEEECCHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHH
RVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAV
HHHHHCCCCCHHHHHHHHHHCCHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHH
VDALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETR
HHHHHCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AADVRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERA
HHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVAS
HCCC
>Mature Secondary Structure 
PNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAA
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHH
WAGGEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTR
CCCCCEEECCCCCCHHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
MNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGEAQAVVALVDALVAERGFDAS
HHHHHHHCCEEEEECCHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHH
RVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAV
HHHHHCCCCCHHHHHHHHHHCCHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHH
VDALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETR
HHHHHCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AADVRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERA
HHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVAS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]