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The map label for this gene is budB [H]

Identifier: 126447062

GI number: 126447062

Start: 209373

End: 211013

Strand: Direct

Name: budB [H]

Synonym: BMA10247_A0246

Alternate gene names: 126447062

Gene position: 209373-211013 (Clockwise)

Preceding gene: 126445648

Following gene: 126447768

Centisome position: 8.9

GC content: 65.02

Gene sequence:

>1641_bases
ATGAAAGCATCCGATCTGTTCGTGAAGGCGCTGGAGGCGGAAGGCGTCCAGTATGTGTTCGGCATTCCCGGCGAGGAAAA
CCTCGATCTGCTCGAATCGCTGCGGCGATCCCGCATCAAGCTCGTGTTGACCCGCCACGAGCAGGCGGCGGGCTTCATGG
CGGCCACCTATGGGCGGCTGACCGGCAAGACGGGCGTGTGCCTCGCGACGCTCGGGCCCGGCGCGACGAACTTCGTCACC
GCGGCCGCGTATGCGCAGCTCGGCGGCATGCCGATGCTGATGATCACCGGGCAAAAGCCGATCAAGTCGAGCAAGCAGGG
GCACTTCCAGATCGTCGACGTCGTCGGGATGATGCAGCCGCTCACGAAGCTCACGCGGCAGATCGTGTCGATCGGCAATA
TTCCGTCGGCGGTGCGCGAGGCGTTCCGGCGCGCGGAGGAGGAACGCCCCGGCGCGACGCATCTCGAATTGCCCGAGGAC
ATCGCGCACGAGGAGGGCGACGGCAAGCCGATTCCGGCGAGTTACAGCCGCCGGCCGATCGCCGAGGAAAAGGCGCTCGC
GCGCGCGGTGGACGCGATCCAGGCGGCGCGCCACCCGCTGCTGATGATCGGCGCGGGCGGCAATCGCAAGACGACCTGCA
GGATGCTCAAGGAGTTCGTCGACACGACGGGCATTCCGTTCTTCACGACGCAGATGGGCAAGGGCGTGATCGACGAGACG
CATCCGCTGTGGCTCGGCAACGCGACGCTGTCGGACGGCGATTTCGTTCACCGCGCGATCGAGCACGCCGATTGCATCGT
CAATGTCGGCCACGACGTGATCGAGAAGCCGCCGTTCTTCATGCGCGCCGACGACAAGACCGTGATCCACGTGAACTTCC
TCGGCGCGCAGGTCGACCCCGTCTACTTTCCGCAGATCGAAGTCGTCGGCGACATCGCGAACGCGGTGTGGCAGATGAAG
GAGAAGCTCGCGCGGCAGCCGCACTGGGATTTCTCGCGCTTCATGCTGATCAAGGCGCACTTCGACGCGCACCTGAAGAA
GGGGCAGGACGACCCGCGCTTTCCGATGTACCCGGTGCGCATCGTCAACGATCTGTACCGGTCGCTGCCGGAGGACGGCA
TCGTATGCCTCGACAACGGTATGTACAAGATCTGGTTCGCGCGCTACTGGCGCGCGCACGAGCCGAATTCGCTGCTGCTC
GACAACGCGCTCGCGTCGATGGGCGCGGGGCTGCCGTCGGCGATCGCGACGAAGATCGTGCATCCGCAGCGCAAGGTGAT
CGCCGTGTGCGGCGACGGCGGCTTCATGATGAATTCTCAGGAGCTCGAAACGGCGGTGCGGCTCAAGCTCGATCTCGTCG
TGATGATCCTGCGCGACGATGCGTTCGGGATGATCCGCTGGAAGCAGGAGAACATGAATTTTCCCGATTACGCGATGACG
CTCGCGAACCCGGATTTCGTCGCGTATGCGCAGAGCTACGGCGCGCACGGGCACCGGATCGCGTCGGCCGACGCGCTCGA
GCCGCTCGTGCGCGAGTGCTTCGCGTCGCCGGGCGTGCACCTGATCGACGTGCCGATCGACTACTCGGACAACGAGCGCG
TGCTCAATCGCGAAATCAAGCGCCTGTCGGCGCAGCTGTGA

Upstream 100 bases:

>100_bases
TCGAAGGAAAAAAGGCCCGTGCGCGCCGTAGGCCGGCGCGCGCGTCGCTCAACGCCGCGCGAACCGTAGGCCGGGGGCGC
GGCACCCACCGAGAGTCCCC

Downstream 100 bases:

>100_bases
CGCTGCGGGCAGGCCGCATATCGGCCGGCCGGACGTCGAGCGGGCGTCAACCGGACCTCAACCGGACCTCAACCGGACGG
AGTCATTCGACATGCTGAAG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKASDLFVKALEAEGVQYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRLTGKTGVCLATLGPGATNFVT
AAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQPLTKLTRQIVSIGNIPSAVREAFRRAEEERPGATHLELPED
IAHEEGDGKPIPASYSRRPIAEEKALARAVDAIQAARHPLLMIGAGGNRKTTCRMLKEFVDTTGIPFFTTQMGKGVIDET
HPLWLGNATLSDGDFVHRAIEHADCIVNVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDPVYFPQIEVVGDIANAVWQMK
EKLARQPHWDFSRFMLIKAHFDAHLKKGQDDPRFPMYPVRIVNDLYRSLPEDGIVCLDNGMYKIWFARYWRAHEPNSLLL
DNALASMGAGLPSAIATKIVHPQRKVIAVCGDGGFMMNSQELETAVRLKLDLVVMILRDDAFGMIRWKQENMNFPDYAMT
LANPDFVAYAQSYGAHGHRIASADALEPLVRECFASPGVHLIDVPIDYSDNERVLNREIKRLSAQL

Sequences:

>Translated_546_residues
MKASDLFVKALEAEGVQYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRLTGKTGVCLATLGPGATNFVT
AAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQPLTKLTRQIVSIGNIPSAVREAFRRAEEERPGATHLELPED
IAHEEGDGKPIPASYSRRPIAEEKALARAVDAIQAARHPLLMIGAGGNRKTTCRMLKEFVDTTGIPFFTTQMGKGVIDET
HPLWLGNATLSDGDFVHRAIEHADCIVNVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDPVYFPQIEVVGDIANAVWQMK
EKLARQPHWDFSRFMLIKAHFDAHLKKGQDDPRFPMYPVRIVNDLYRSLPEDGIVCLDNGMYKIWFARYWRAHEPNSLLL
DNALASMGAGLPSAIATKIVHPQRKVIAVCGDGGFMMNSQELETAVRLKLDLVVMILRDDAFGMIRWKQENMNFPDYAMT
LANPDFVAYAQSYGAHGHRIASADALEPLVRECFASPGVHLIDVPIDYSDNERVLNREIKRLSAQL
>Mature_546_residues
MKASDLFVKALEAEGVQYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRLTGKTGVCLATLGPGATNFVT
AAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQPLTKLTRQIVSIGNIPSAVREAFRRAEEERPGATHLELPED
IAHEEGDGKPIPASYSRRPIAEEKALARAVDAIQAARHPLLMIGAGGNRKTTCRMLKEFVDTTGIPFFTTQMGKGVIDET
HPLWLGNATLSDGDFVHRAIEHADCIVNVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDPVYFPQIEVVGDIANAVWQMK
EKLARQPHWDFSRFMLIKAHFDAHLKKGQDDPRFPMYPVRIVNDLYRSLPEDGIVCLDNGMYKIWFARYWRAHEPNSLLL
DNALASMGAGLPSAIATKIVHPQRKVIAVCGDGGFMMNSQELETAVRLKLDLVVMILRDDAFGMIRWKQENMNFPDYAMT
LANPDFVAYAQSYGAHGHRIASADALEPLVRECFASPGVHLIDVPIDYSDNERVLNREIKRLSAQL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=548, Percent_Identity=26.6423357664234, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI21361361, Length=537, Percent_Identity=25.512104283054, Blast_Score=152, Evalue=8e-37,
Organism=Escherichia coli, GI1790104, Length=542, Percent_Identity=29.3357933579336, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI87081685, Length=549, Percent_Identity=28.9617486338798, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1786717, Length=506, Percent_Identity=25.6916996047431, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1787096, Length=531, Percent_Identity=24.4821092278719, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788716, Length=550, Percent_Identity=24.7272727272727, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=578, Percent_Identity=24.5674740484429, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=483, Percent_Identity=24.6376811594203, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=483, Percent_Identity=24.6376811594203, Blast_Score=135, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=28.4974093264249, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6320816, Length=482, Percent_Identity=24.896265560166, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321524, Length=488, Percent_Identity=23.5655737704918, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=548, Percent_Identity=22.4452554744526, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=489, Percent_Identity=22.9038854805726, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922626, Length=482, Percent_Identity=26.3485477178423, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60639; Mature: 60639

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASDLFVKALEAEGVQYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRL
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEECHHHHCHHHHHHHHC
TGKTGVCLATLGPGATNFVTAAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQP
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHH
LTKLTRQIVSIGNIPSAVREAFRRAEEERPGATHLELPEDIAHEEGDGKPIPASYSRRPI
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEEKALARAVDAIQAARHPLLMIGAGGNRKTTCRMLKEFVDTTGIPFFTTQMGKGVIDET
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCC
HPLWLGNATLSDGDFVHRAIEHADCIVNVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDP
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCCEEEEEEEECCCCCC
VYFPQIEVVGDIANAVWQMKEKLARQPHWDFSRFMLIKAHFDAHLKKGQDDPRFPMYPVR
EECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEHHHHHHHCCCCCCCCCCHHHH
IVNDLYRSLPEDGIVCLDNGMYKIWFARYWRAHEPNSLLLDNALASMGAGLPSAIATKIV
HHHHHHHHCCCCCEEEECCCEEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHC
HPQRKVIAVCGDGGFMMNSQELETAVRLKLDLVVMILRDDAFGMIRWKQENMNFPDYAMT
CCCCEEEEEECCCCEEECHHHHHHHHHHEEEEEEEEEECCCCEEEEEECCCCCCCCCEEE
LANPDFVAYAQSYGAHGHRIASADALEPLVRECFASPGVHLIDVPIDYSDNERVLNREIK
ECCCCHHHHHHHCCCCCCEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
RLSAQL
HHHCCC
>Mature Secondary Structure
MKASDLFVKALEAEGVQYVFGIPGEENLDLLESLRRSRIKLVLTRHEQAAGFMAATYGRL
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEECHHHHCHHHHHHHHC
TGKTGVCLATLGPGATNFVTAAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQP
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHH
LTKLTRQIVSIGNIPSAVREAFRRAEEERPGATHLELPEDIAHEEGDGKPIPASYSRRPI
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEEKALARAVDAIQAARHPLLMIGAGGNRKTTCRMLKEFVDTTGIPFFTTQMGKGVIDET
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCC
HPLWLGNATLSDGDFVHRAIEHADCIVNVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDP
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCCEEEEEEEECCCCCC
VYFPQIEVVGDIANAVWQMKEKLARQPHWDFSRFMLIKAHFDAHLKKGQDDPRFPMYPVR
EECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEHHHHHHHCCCCCCCCCCHHHH
IVNDLYRSLPEDGIVCLDNGMYKIWFARYWRAHEPNSLLLDNALASMGAGLPSAIATKIV
HHHHHHHHCCCCCEEEECCCEEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHC
HPQRKVIAVCGDGGFMMNSQELETAVRLKLDLVVMILRDDAFGMIRWKQENMNFPDYAMT
CCCCEEEEEECCCCEEECHHHHHHHHHHEEEEEEEEEECCCCEEEEEECCCCCCCCCEEE
LANPDFVAYAQSYGAHGHRIASADALEPLVRECFASPGVHLIDVPIDYSDNERVLNREIK
ECCCCHHHHHHHCCCCCCEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
RLSAQL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]