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The map label for this gene is proW [H]

Identifier: 126447053

GI number: 126447053

Start: 1799464

End: 1800366

Strand: Reverse

Name: proW [H]

Synonym: BMA10247_A1869

Alternate gene names: 126447053

Gene position: 1800366-1799464 (Counterclockwise)

Preceding gene: 126447578

Following gene: 126447174

Centisome position: 76.52

GC content: 67.66

Gene sequence:

>903_bases
ATGTCTGAACTCATTCCGCTCGGTAGCTGGGTCGACCAGTCGGTTCACTATCTGCTCGACCACGACGCGAAGACGTTCGA
CGCGATCGGCCGCGCCATCGAGGGGCTCGCCGCGCTCGTCGAGCACGGCCTGCAGGCCGTGCCGATGTGGCTGATGATGG
CGTGCTTCATCGGCGTCGGCCTCTGGCGCGTGGGCTGGCGCTTCGCGTTCTTCACCGCCGCGTCGCTGTTCCTGATCTTC
GCGACGGGCTTCTGGGATCAGACAGTCGTCACGCTCGGCCTCACGCTGTCGTCGACGATCGTGAGCCTCGTGCTCGGCAT
TCCGCTCGGCATCTGGGCCGCGAAGAGCAAGTGGGTCGCGGCGATCGTGCGGCCGATCCTCGACCTGATGCAGACGATGC
CCGCGTTCGTCTACCTGATTCCGGCCGCGATGCTGTTCGGCCTCGGCCGCGTGCCGGGGATTCTGTCGACGGTGATCTTC
GCGATGCCGCCCGCCGTGCGCCTGACGAGCCTCGGCATCCGCCACGTGAACCGCGAGATCGTCGAGGCGGGGCAGGCGTT
CGGCTGCACGCCGTGGCAACTGCTGTACAAGGTGCAGTTCCCGAATGCGCTGCCGTCGATCATGCAGGGCGTGAACCAGA
CGATCATGATGGCGCTGTCGATGGTGATCATCGCGTCGATGGTCGGCGCGGGCGGCCTCGGCAACGACGTGCTCGCGAGC
ATCCAGCGCCTGGACATCGGCTTGGGCTTCGAGAGCGGCCTGTCGGTCGTGCTGCTCGCGATCATCCTCGACCGCATCAC
CGAGAGCTTCGGCCGCGCGCCGGGCACGGCGAGAGCGCCGCTCTTCTCGGGGCTGCGGCAACTGTTCGGCCCGCGCTCGC
GCTCGCCGGTCGTGCAGGCCTGA

Upstream 100 bases:

>100_bases
CATCCGCACGCGCTGCCCGTCGTCGACGACGACGGCTGTTACTGCGGCTCGGTCGACCGGGCCGTGCTTCTGAAAGCCAT
TACGCGTTCACGAGGTTCCC

Downstream 100 bases:

>100_bases
CGTTTCCCGCTTTCCGGCGTTTCGCCGGTTTTTTCGCCTTCCTCGTTTCGCCGATCCGGTCGTCTCCGGCGGGCTGTTCG
CCGCCGGAGCGGCTTCGCGC

Product: glycine betaine/L-proline ABC transporter, permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSELIPLGSWVDQSVHYLLDHDAKTFDAIGRAIEGLAALVEHGLQAVPMWLMMACFIGVGLWRVGWRFAFFTAASLFLIF
ATGFWDQTVVTLGLTLSSTIVSLVLGIPLGIWAAKSKWVAAIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIF
AMPPAVRLTSLGIRHVNREIVEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGTARAPLFSGLRQLFGPRSRSPVVQA

Sequences:

>Translated_300_residues
MSELIPLGSWVDQSVHYLLDHDAKTFDAIGRAIEGLAALVEHGLQAVPMWLMMACFIGVGLWRVGWRFAFFTAASLFLIF
ATGFWDQTVVTLGLTLSSTIVSLVLGIPLGIWAAKSKWVAAIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIF
AMPPAVRLTSLGIRHVNREIVEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGTARAPLFSGLRQLFGPRSRSPVVQA
>Mature_299_residues
SELIPLGSWVDQSVHYLLDHDAKTFDAIGRAIEGLAALVEHGLQAVPMWLMMACFIGVGLWRVGWRFAFFTAASLFLIFA
TGFWDQTVVTLGLTLSSTIVSLVLGIPLGIWAAKSKWVAAIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFA
MPPAVRLTSLGIRHVNREIVEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLASI
QRLDIGLGFESGLSVVLLAIILDRITESFGRAPGTARAPLFSGLRQLFGPRSRSPVVQA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=271, Percent_Identity=46.4944649446494, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1788451, Length=201, Percent_Identity=28.8557213930348, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788449, Length=180, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32308; Mature: 32177

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELIPLGSWVDQSVHYLLDHDAKTFDAIGRAIEGLAALVEHGLQAVPMWLMMACFIGVG
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWRVGWRFAFFTAASLFLIFATGFWDQTVVTLGLTLSSTIVSLVLGIPLGIWAAKSKWVA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFAMPPAVRLTSLGIRHVNREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
HHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGTARAPLFSGLRQLFGPRSRSPVVQA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SELIPLGSWVDQSVHYLLDHDAKTFDAIGRAIEGLAALVEHGLQAVPMWLMMACFIGVG
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]