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The map label for this gene is pilT [H]

Identifier: 126446329

GI number: 126446329

Start: 605953

End: 607059

Strand: Reverse

Name: pilT [H]

Synonym: BMA10247_A0656

Alternate gene names: 126446329

Gene position: 607059-605953 (Counterclockwise)

Preceding gene: 126445976

Following gene: 126446662

Centisome position: 25.8

GC content: 66.4

Gene sequence:

>1107_bases
ATGAACCAGCATGCCGCCGCCGCCCCGCTGCGCGATCTCGCGGGCGAAGTGCTCGGCCTGATCGACCTGAAGCAGATGTT
TACCGACATCCACATCGAACAGGACCGCCCCGTCACGATCAAGACGCCGCGCGGCTGGGTCGAGACGAGCGACGAACCGG
TGCTCCTCGACGACATGCGCCCTCTGCTCGATGCGATCGACGAGCACTGGGAGAGCGCGCTGCGCCAGGGCACGCTCGAC
CGCCCGTTCGTCCTCACGCGCTGCCGGCTGCGCTGCCACGTGTATCGCGCGGGCGGCGGGCGCAAGATCGTGATCTCGAT
GCGGCGGCTGCCGCTCAAGCCGCTGCCGCTCGAGGAGCTCGGCCTGCCCGTCTACGTGCGCTCGATGCTCGACAACACGA
AGGGCATCATCCTCGTCACCGGCCCGACGGGCTCCGGCAAGACGACGACGATCGCTTCGCTGCTCGAGCACGTCAACGCG
ACGCGCAACACGCATATCGTCACGATCGAGGAACCGATCGAATACTATCTGGAGCGCCGCCAGTCGATCATCTCGCAGCG
CGAAGTGCCGACCGACACGCCGAATTTCCCGCACGGGCTGCGCGAGGCGCTGCGGCAGAAACCGGACGTCGTGATGGTGG
GCGAGGTGCGCGACGCGCAGACGGCCGACACGCTGCTGCAGGCGGGCGAATCCGGGCACCTCGTGCTCGCGACGATGCAC
ACGGGCAGCGCGGTCAGCGCGCTCAACAAGCTGCTGTCGTTCTTTCCGGTCGAGCAGCGCGACCGGCACGCGGTCGCGCT
CGCCGAATCGCTGATCGGCATCGTCTGCCAGAGCCTCGTGCCGAGCGCGGACGGCGAAAGCCTCGTGCTCGCGAGCGAGC
TGCTGTTCAACAACAACAACCAGGTCGCAAAGCTGATCGCGGACCCGACCAAGCTGCACCTCGTCAACGAGTTCCTGCGC
CGCCGGGAGGACAACATGTCGCGCTCGCTGAACGACGACCTCGCGGCGCTCGCGGCCCGCAACCGGATCACGACGAAGGA
TGCGTTACGGGCCACTTACAATCGGCTCGAGCTGCATGAAATGATGCAGTCGATTGTTAATCGATAG

Upstream 100 bases:

>100_bases
CAAAAGCTAGTTGGCATGCAAAATTCAATAGACCATTCAATTTCCCACTCGCCGCGCGCGAAATGCGCCGTCCACTCCAT
TCATTGGAATCGCCACCAAG

Downstream 100 bases:

>100_bases
CGACCGCGCGAAGCGGGCCCGGCGTTTTTATACATCGATCTAAAATCTGAAAAAAATCATATAAACAAGCGGCGCCCCCG
CGTTAATCGACTGGACGCGT

Product: putative twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNQHAAAAPLRDLAGEVLGLIDLKQMFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMRPLLDAIDEHWESALRQGTLD
RPFVLTRCRLRCHVYRAGGGRKIVISMRRLPLKPLPLEELGLPVYVRSMLDNTKGIILVTGPTGSGKTTTIASLLEHVNA
TRNTHIVTIEEPIEYYLERRQSIISQREVPTDTPNFPHGLREALRQKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMH
TGSAVSALNKLLSFFPVEQRDRHAVALAESLIGIVCQSLVPSADGESLVLASELLFNNNNQVAKLIADPTKLHLVNEFLR
RREDNMSRSLNDDLAALAARNRITTKDALRATYNRLELHEMMQSIVNR

Sequences:

>Translated_368_residues
MNQHAAAAPLRDLAGEVLGLIDLKQMFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMRPLLDAIDEHWESALRQGTLD
RPFVLTRCRLRCHVYRAGGGRKIVISMRRLPLKPLPLEELGLPVYVRSMLDNTKGIILVTGPTGSGKTTTIASLLEHVNA
TRNTHIVTIEEPIEYYLERRQSIISQREVPTDTPNFPHGLREALRQKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMH
TGSAVSALNKLLSFFPVEQRDRHAVALAESLIGIVCQSLVPSADGESLVLASELLFNNNNQVAKLIADPTKLHLVNEFLR
RREDNMSRSLNDDLAALAARNRITTKDALRATYNRLELHEMMQSIVNR
>Mature_368_residues
MNQHAAAAPLRDLAGEVLGLIDLKQMFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMRPLLDAIDEHWESALRQGTLD
RPFVLTRCRLRCHVYRAGGGRKIVISMRRLPLKPLPLEELGLPVYVRSMLDNTKGIILVTGPTGSGKTTTIASLLEHVNA
TRNTHIVTIEEPIEYYLERRQSIISQREVPTDTPNFPHGLREALRQKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMH
TGSAVSALNKLLSFFPVEQRDRHAVALAESLIGIVCQSLVPSADGESLVLASELLFNNNNQVAKLIADPTKLHLVNEFLR
RREDNMSRSLNDDLAALAARNRITTKDALRATYNRLELHEMMQSIVNR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=36.8794326241135, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1789723, Length=173, Percent_Identity=45.6647398843931, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1786296, Length=158, Percent_Identity=37.9746835443038, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41092; Mature: 41092

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQHAAAAPLRDLAGEVLGLIDLKQMFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCEEHHHHH
PLLDAIDEHWESALRQGTLDRPFVLTRCRLRCHVYRAGGGRKIVISMRRLPLKPLPLEEL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCHHHC
GLPVYVRSMLDNTKGIILVTGPTGSGKTTTIASLLEHVNATRNTHIVTIEEPIEYYLERR
CCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
QSIISQREVPTDTPNFPHGLREALRQKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMH
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEC
TGSAVSALNKLLSFFPVEQRDRHAVALAESLIGIVCQSLVPSADGESLVLASELLFNNNN
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCC
QVAKLIADPTKLHLVNEFLRRREDNMSRSLNDDLAALAARNRITTKDALRATYNRLELHE
CEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
MMQSIVNR
HHHHHHCC
>Mature Secondary Structure
MNQHAAAAPLRDLAGEVLGLIDLKQMFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCEEHHHHH
PLLDAIDEHWESALRQGTLDRPFVLTRCRLRCHVYRAGGGRKIVISMRRLPLKPLPLEEL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCHHHC
GLPVYVRSMLDNTKGIILVTGPTGSGKTTTIASLLEHVNATRNTHIVTIEEPIEYYLERR
CCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
QSIISQREVPTDTPNFPHGLREALRQKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMH
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEC
TGSAVSALNKLLSFFPVEQRDRHAVALAESLIGIVCQSLVPSADGESLVLASELLFNNNN
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCC
QVAKLIADPTKLHLVNEFLRRREDNMSRSLNDDLAALAARNRITTKDALRATYNRLELHE
CEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
MMQSIVNR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]