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The map label for this gene is alkK [H]

Identifier: 126446078

GI number: 126446078

Start: 1431712

End: 1433373

Strand: Reverse

Name: alkK [H]

Synonym: BMA10247_A1474

Alternate gene names: 126446078

Gene position: 1433373-1431712 (Counterclockwise)

Preceding gene: 126447951

Following gene: 126447128

Centisome position: 60.92

GC content: 69.8

Gene sequence:

>1662_bases
ATGACGCAGAAGTTCGAGGAGGGGCTCGGCAGGCGCGAGGCCAACTACGTGCCGCTCACGCCGATCGATTTCATCGCGCG
CGCGGCGGAGGTGTACGGCGAGCGCCTCGCCGCCGTGCACGGCGAGATCCGCCGCACGTGGAGCGAGACCTACGCGCGCG
CGCGACGGCTCGCGAGCGCGCTCGAGCGCGCGGGCGTCGGGCGGGGCGACACCGTCGCCGCGCTGCTGCCGAACATTCCG
GCGATGATCGAGGCGCACTTCGGCGTGCCGATGGCGGGCGCCGTCCTCAACGCGCTGAACACGCGGCTCGACGTCGCGTC
GATGCTGTTCATGCTGCGTCACGGCGAGGCGAAGGTGCTGATCGTCGATACCGAGTACGCGGAGTTCGCGCACCGCGCGG
CGCTCGAGGTTCCGGGGCTCGCGATCGTCAGCGTGGCCGACGCGATGCCCGCCGATCCCGCGCGCTTTCCCGCGGCGCTC
GATTACGAGGCGTTCCTCGCCGGCGGCGATCCCGCGTACGCCTGGACGCCGCCGTCCGACGAATGGGAGGCGATCGCGCT
GAATTACACGTCGGGGACGACGGGCGATCCGAAGGGCGTCGTCTACCATCATCGCGGCGCGTATCTCGCCGCGATCAGCA
ACCTCCTCGAATGGGACATGCCGAAGCACGCGGTCTACCTGTGGACGCTGCCGATGTTCCACTGCAACGGCTGGTGCTTT
CCGTGGGCGGTGGCCGCGCGCGCGGGCGTGAACGTGTGCCTGCGCAAGTTCGACGCGAAGCGCGTGCTCGAGCTGATCCG
CCGCGAGCGTGTCACGCACTACAGCGGCGCGCCGATCGTGCAGAGCGCGATCGCGAACGCGCCGGCCGAATGGCGCGCGG
GGATCGCGCACGAGGTGCATGCGTTCGTCGCGGGCGCCGCGCCCGCGCCGGCCGTGATCGCGAAGATGAAGGAGATCGGC
TTCGACCTCACGCATGTCTACGGGCTCACCGAAGTCTACGGGCCCGCGACGGTCTGCGCGAAGCAGGCGCACTGGGCGTC
GCTGCCCGACGACGAGCTCGCGCAGCTCAACGCGCGGCAGGGCGTGCGCTACCACCTGCAGGCGGGCGCGACCGTGCTTG
ATCCCGACACGATGGCGCCCGTGCCGGCGGACGGCGAGACGCTCGGCGAGATCATGTTCCGCGGCAACCTCTGCATGAAG
GGCTACCTGAAGAACCCGCACGCGACCGACGAGGCGTTCGCGGGCGGCTGGTTCCACACGGGGGACCTCGGCGTGCTGAC
GCCCGACGGCTACATCCGCATCAAGGATCGCCGCAAGGACATCATCATCTCGGGCGGCGAGAACATCTCCAGCATCGAGG
TCGAGGACGCGCTGTACCGGCATCCGGCCGTCGAGGTCGCGGCCGTCGTCGCGCTGCCGGACCCGAAGTGGGGCGAGGTG
CCGTGCGCGTTCGTCGAGCTGCGCGACGGGATGAGCGCGACCGAGGAGGAGATCATCGCGCACTGCCGGCTGCTGCTCGC
CGCGTACAAGATTCCGAAGACGGTGCGCTTCGGTGAGCTGCCGAAGACATCGACCGGGAAGATCCAGAAATTTCAATTGC
GCGCGCAGGTCGGGTCGAGCTCGGCGATCGATCTGGTGGCGGCGGCCAGGAAGCCAGGGTAG

Upstream 100 bases:

>100_bases
CGCTTGCATGACAGACGGCTCACAATGGCCGCGAGTAGGCTTGTCCAATTGGACCACAAACCAACGACATCGAGGCGCAC
GCCTTGCAGGAGACGACATC

Downstream 100 bases:

>100_bases
GCGCGACGCGTCGCGCGGTCACGTGACGGGCGGCGAGCGGCCGTTGCGGGCCGCGTGCGCCGCGCGCTCCCCGCCTGCCG
GGCACGTGGCGCTCGGCGTT

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTQKFEEGLGRREANYVPLTPIDFIARAAEVYGERLAAVHGEIRRTWSETYARARRLASALERAGVGRGDTVAALLPNIP
AMIEAHFGVPMAGAVLNALNTRLDVASMLFMLRHGEAKVLIVDTEYAEFAHRAALEVPGLAIVSVADAMPADPARFPAAL
DYEAFLAGGDPAYAWTPPSDEWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNLLEWDMPKHAVYLWTLPMFHCNGWCF
PWAVAARAGVNVCLRKFDAKRVLELIRRERVTHYSGAPIVQSAIANAPAEWRAGIAHEVHAFVAGAAPAPAVIAKMKEIG
FDLTHVYGLTEVYGPATVCAKQAHWASLPDDELAQLNARQGVRYHLQAGATVLDPDTMAPVPADGETLGEIMFRGNLCMK
GYLKNPHATDEAFAGGWFHTGDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVEVAAVVALPDPKWGEV
PCAFVELRDGMSATEEEIIAHCRLLLAAYKIPKTVRFGELPKTSTGKIQKFQLRAQVGSSSAIDLVAAARKPG

Sequences:

>Translated_553_residues
MTQKFEEGLGRREANYVPLTPIDFIARAAEVYGERLAAVHGEIRRTWSETYARARRLASALERAGVGRGDTVAALLPNIP
AMIEAHFGVPMAGAVLNALNTRLDVASMLFMLRHGEAKVLIVDTEYAEFAHRAALEVPGLAIVSVADAMPADPARFPAAL
DYEAFLAGGDPAYAWTPPSDEWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNLLEWDMPKHAVYLWTLPMFHCNGWCF
PWAVAARAGVNVCLRKFDAKRVLELIRRERVTHYSGAPIVQSAIANAPAEWRAGIAHEVHAFVAGAAPAPAVIAKMKEIG
FDLTHVYGLTEVYGPATVCAKQAHWASLPDDELAQLNARQGVRYHLQAGATVLDPDTMAPVPADGETLGEIMFRGNLCMK
GYLKNPHATDEAFAGGWFHTGDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVEVAAVVALPDPKWGEV
PCAFVELRDGMSATEEEIIAHCRLLLAAYKIPKTVRFGELPKTSTGKIQKFQLRAQVGSSSAIDLVAAARKPG
>Mature_552_residues
TQKFEEGLGRREANYVPLTPIDFIARAAEVYGERLAAVHGEIRRTWSETYARARRLASALERAGVGRGDTVAALLPNIPA
MIEAHFGVPMAGAVLNALNTRLDVASMLFMLRHGEAKVLIVDTEYAEFAHRAALEVPGLAIVSVADAMPADPARFPAALD
YEAFLAGGDPAYAWTPPSDEWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNLLEWDMPKHAVYLWTLPMFHCNGWCFP
WAVAARAGVNVCLRKFDAKRVLELIRRERVTHYSGAPIVQSAIANAPAEWRAGIAHEVHAFVAGAAPAPAVIAKMKEIGF
DLTHVYGLTEVYGPATVCAKQAHWASLPDDELAQLNARQGVRYHLQAGATVLDPDTMAPVPADGETLGEIMFRGNLCMKG
YLKNPHATDEAFAGGWFHTGDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVEVAAVVALPDPKWGEVP
CAFVELRDGMSATEEEIIAHCRLLLAAYKIPKTVRFGELPKTSTGKIQKFQLRAQVGSSSAIDLVAAARKPG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=548, Percent_Identity=29.5620437956204, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI187761345, Length=543, Percent_Identity=28.3609576427256, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI187761343, Length=543, Percent_Identity=28.3609576427256, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI58082049, Length=513, Percent_Identity=26.9005847953216, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI157311624, Length=510, Percent_Identity=26.2745098039216, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI157311622, Length=510, Percent_Identity=26.2745098039216, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI122937307, Length=514, Percent_Identity=25.875486381323, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI38505220, Length=511, Percent_Identity=27.0058708414873, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI115511026, Length=512, Percent_Identity=25.1953125, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI42544132, Length=513, Percent_Identity=26.5107212475634, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI83745141, Length=534, Percent_Identity=20.7865168539326, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13375727, Length=180, Percent_Identity=34.4444444444444, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI28416953, Length=130, Percent_Identity=31.5384615384615, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788107, Length=555, Percent_Identity=26.6666666666667, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI221142682, Length=528, Percent_Identity=27.8409090909091, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI145693145, Length=538, Percent_Identity=29.5539033457249, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1786810, Length=569, Percent_Identity=26.713532513181, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788595, Length=461, Percent_Identity=26.6811279826464, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=531, Percent_Identity=28.4369114877589, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71994694, Length=531, Percent_Identity=28.4369114877589, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71994690, Length=531, Percent_Identity=28.4369114877589, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17560308, Length=532, Percent_Identity=28.3834586466165, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17558820, Length=378, Percent_Identity=32.2751322751323, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=527, Percent_Identity=25.9962049335863, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17538037, Length=522, Percent_Identity=26.0536398467433, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=514, Percent_Identity=25.2918287937743, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=509, Percent_Identity=25.7367387033399, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=481, Percent_Identity=24.5322245322245, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=530, Percent_Identity=26.2264150943396, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=545, Percent_Identity=23.8532110091743, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=561, Percent_Identity=23.3511586452763, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71982997, Length=561, Percent_Identity=23.3511586452763, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71985884, Length=151, Percent_Identity=35.7615894039735, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=488, Percent_Identity=26.2295081967213, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323182, Length=146, Percent_Identity=33.5616438356164, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319264, Length=207, Percent_Identity=29.951690821256, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24581924, Length=541, Percent_Identity=27.7264325323475, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21355181, Length=528, Percent_Identity=27.4621212121212, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI18859661, Length=564, Percent_Identity=26.063829787234, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648260, Length=513, Percent_Identity=23.3918128654971, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356947, Length=513, Percent_Identity=21.6374269005848, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24583465, Length=567, Percent_Identity=23.8095238095238, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24583463, Length=567, Percent_Identity=23.8095238095238, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281364798, Length=567, Percent_Identity=23.8095238095238, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI20130357, Length=356, Percent_Identity=25, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358303, Length=479, Percent_Identity=22.5469728601253, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648257, Length=556, Percent_Identity=23.2014388489209, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648253, Length=531, Percent_Identity=21.8455743879473, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281365686, Length=489, Percent_Identity=22.0858895705521, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648255, Length=529, Percent_Identity=21.5500945179584, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60014; Mature: 59883

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKFEEGLGRREANYVPLTPIDFIARAAEVYGERLAAVHGEIRRTWSETYARARRLASA
CCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERAGVGRGDTVAALLPNIPAMIEAHFGVPMAGAVLNALNTRLDVASMLFMLRHGEAKVL
HHHCCCCCCCCHHHHHCCCHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IVDTEYAEFAHRAALEVPGLAIVSVADAMPADPARFPAALDYEAFLAGGDPAYAWTPPSD
EEECHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCC
EWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNLLEWDMPKHAVYLWTLPMFHCNGWCF
CCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCH
PWAVAARAGVNVCLRKFDAKRVLELIRRERVTHYSGAPIVQSAIANAPAEWRAGIAHEVH
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
AFVAGAAPAPAVIAKMKEIGFDLTHVYGLTEVYGPATVCAKQAHWASLPDDELAQLNARQ
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHC
GVRYHLQAGATVLDPDTMAPVPADGETLGEIMFRGNLCMKGYLKNPHATDEAFAGGWFHT
CCEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEE
GDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVEVAAVVALPDPKWGEV
CCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCCEEEEEEEECCCCCCCCC
PCAFVELRDGMSATEEEIIAHCRLLLAAYKIPKTVRFGELPKTSTGKIQKFQLRAQVGSS
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
SAIDLVAAARKPG
CHHHHHHHCCCCC
>Mature Secondary Structure 
TQKFEEGLGRREANYVPLTPIDFIARAAEVYGERLAAVHGEIRRTWSETYARARRLASA
CCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERAGVGRGDTVAALLPNIPAMIEAHFGVPMAGAVLNALNTRLDVASMLFMLRHGEAKVL
HHHCCCCCCCCHHHHHCCCHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IVDTEYAEFAHRAALEVPGLAIVSVADAMPADPARFPAALDYEAFLAGGDPAYAWTPPSD
EEECHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCC
EWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNLLEWDMPKHAVYLWTLPMFHCNGWCF
CCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCH
PWAVAARAGVNVCLRKFDAKRVLELIRRERVTHYSGAPIVQSAIANAPAEWRAGIAHEVH
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
AFVAGAAPAPAVIAKMKEIGFDLTHVYGLTEVYGPATVCAKQAHWASLPDDELAQLNARQ
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHC
GVRYHLQAGATVLDPDTMAPVPADGETLGEIMFRGNLCMKGYLKNPHATDEAFAGGWFHT
CCEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEE
GDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVEVAAVVALPDPKWGEV
CCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCCEEEEEEEECCCCCCCCC
PCAFVELRDGMSATEEEIIAHCRLLLAAYKIPKTVRFGELPKTSTGKIQKFQLRAQVGSS
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
SAIDLVAAARKPG
CHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]