Click here to switch to the map view.

The map label for this gene is ptrB [C]

Identifier: 126446051

GI number: 126446051

Start: 1374532

End: 1376628

Strand: Direct

Name: ptrB [C]

Synonym: BMA10247_A1414

Alternate gene names: 126446051

Gene position: 1374532-1376628 (Clockwise)

Preceding gene: 262192851

Following gene: 262192891

Centisome position: 58.42

GC content: 68.34

Gene sequence:

>2097_bases
ATGCCTCATGCTTCCTGGCCCGAACAGGCCGATCCGCATCAATTCCTCGAAGAACTGGACAGCGCCGCGAGCGTCGGCTG
GGTCGACGCGCAAAACGCCCGCACGCACGATGCGCCCTGGCTCGACGAAGCGCACTATCGCGCGCTGGTCGAGCGCTTCA
CCCAGGCGCTGCTGCCGCGCGAGCGCCCGGTGATTCCGCAGCGCTGGCAGGACTGGGCGTACGACGTCTGGCAGGACGAA
CAGCATCCGAAGGGCCTGTGGCGGCGCACGCGATGGACGAGCTGGCGCAGCGGCCACGCGGACTGGCAGACGTTGATCGA
CCTCGATGCGCTTGGTGAAGCGCAAGGCGTGCAGTGGGTGTTCGACGATCAGCTCATCCTCGAGCCGGACGGCGATCGTG
CGCTGATCGTGCTGTCCGACGGCGGCGCCGACGCGGTCGTCGTCCGCGAGTTCGACATCGCGCAATGCCGGTTCGTCGAC
GACGGCTTCTCGATCGAAGCGGCCGGCAAGCATTCGGTCGAATGGATCGATCGCGACACGATCTACGTCGGCTGGGACGA
CGGCGGCGCCACCGTCACGCGCTCCGGCTATCCGCGCGAAGTGCGGCGCTGGACGCGCGGCACGCCGCTGTCCAGCGCGC
CCGTGGTGTTTCGCGGCGCGCGCGGCGACATCTCGGTCGATGCGCAATATGATCCGCTCGACCGGCATCACGCGATCGAG
CAGGCGATCAATTTCTACGACGCGAACACGTATCGCCTCGCCGAGGACGGCGCGTGGGCGCGCTACGACGTGCCACCGCA
CGTCGAAGTCGGTTACTGGAGCGGGTGGCTGCTGCTCCAGCCGCGGCTCGACTGGACTTGCGGCGGCGCGCGCTACGCGG
GCGGCAGCCTGCTCGCGATCCGCGAGGACGCGTTCGTCGCCGGTGAGCGCGCGTTCGCCGCGCTGTTCGAGCCGAACGAG
CGCACGTCCGCATGCGGCTGGACGCACACGCGCCGCTACGTGCTGGTGTCGTGGCTCGACGACGTGCTCACGCGCACGAT
GCTCTGGCTTCCCGAACGTCAGGATGACGGAGCATGGCGCTGGCATGCTCGTCCGTTCCCCGCGCGAGGGCTCGCGCAAG
TGGACGTGTCGCCCGTCGAGCCCACGTTCGACGACGAGGTGTACGTGAGCGTCGACGATTACCTGAAGCCGCCCGAGTAT
TCGCTCGCGAATCTCGCCAGCGACGACCTGTCCGCCTGGACGCTGCTCGACCGCTGGCCGACGCAGTTCGACGCGTCCGA
ACTGACGGTGCGGCGCGAACACGCGCGCTCGCGCGACGGCACGCTCGTGCCTTATACGCTGGTCGGGCCGCGCGACGTGC
TGGACAATGCGGCGCGCGCGCCGCGCCCCTGCCTGTTGAACGGCTACGGCGGCTTCGCGATTGCGCTCACGCCCGATTAC
GATCCGTTGCTCGGCATCGGCTGGCTCGAGAAAGGCGGCATCGCGGTGTTCGCCCATATTCGCGGCGGCGGCGAGTTCGG
CACGCAGTGGCACGAATCGGCGCGGCAAACGCAACGGCAGCGATCGTTCGACGATTTCATCGCGGTCGCCGAAAAACTCG
TCGCGGACGGCGTGACGAGCGCCGCGCAACTGGGTATTCGCGGCGGCAGCAACGGCGGGCTGCTGGTCGCGGCATGCATG
ATTCAGCGCCCGGACCTGTTCGGCGCGGTGGTGAGCGACGTGCCGCTTCTCGACATGCAGCGCTATGCGCTGCTGCACGC
GGGCGCATCGTGGCTGGACGAATTCGGCGATCCCGACGATCCGGCGCATGCGTCGGCGCTCGCGGCCTACTCGCCGTATC
ACCGGGTCGCGCGCGACATCGCGTATCCGCCCGCGCTGTTCACGACATCGACGAGCGACGACCGCGTGCATCCCGCCCAT
GCGAGAAAAATGGTCGCGCGCATGCAGGCGCAAGGGCACCGAAACGTATGGCTGATCGAGAAAACCGATGGCGGCCACGG
CAGCGCGGACGCGATCGATACCGCCGAGCACGAAGCGATCGGCTATGTGTTTCTGTGGACTCACTTGTCCCGCGGCGCGC
ATGACGCGCGCGAGTGA

Upstream 100 bases:

>100_bases
GCCCGCGGCGGACGCGTCGCAATCGACGAGCCGGCAGCCAAGCGCGAGCGCCGCTCGTCTTACGCTACCATTACACGCCA
TGCCCCTTGCAAAGAACGCC

Downstream 100 bases:

>100_bases
ATCGCCGTGACGGTGCGTGCGGTCGCTCGCCCCGTCTTTCCTGCATGCGGGGCGGATCGACGTTGCCGCCACGTCGGATC
GCAGCAGGCGCGACGGCGCG

Product: prolyl oligopeptidase family protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MPHASWPEQADPHQFLEELDSAASVGWVDAQNARTHDAPWLDEAHYRALVERFTQALLPRERPVIPQRWQDWAYDVWQDE
QHPKGLWRRTRWTSWRSGHADWQTLIDLDALGEAQGVQWVFDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQCRFVD
DGFSIEAAGKHSVEWIDRDTIYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVVFRGARGDISVDAQYDPLDRHHAIE
QAINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGWLLLQPRLDWTCGGARYAGGSLLAIREDAFVAGERAFAALFEPNE
RTSACGWTHTRRYVLVSWLDDVLTRTMLWLPERQDDGAWRWHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLKPPEY
SLANLASDDLSAWTLLDRWPTQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDNAARAPRPCLLNGYGGFAIALTPDY
DPLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQTQRQRSFDDFIAVAEKLVADGVTSAAQLGIRGGSNGGLLVAACM
IQRPDLFGAVVSDVPLLDMQRYALLHAGASWLDEFGDPDDPAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRVHPAH
ARKMVARMQAQGHRNVWLIEKTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDARE

Sequences:

>Translated_698_residues
MPHASWPEQADPHQFLEELDSAASVGWVDAQNARTHDAPWLDEAHYRALVERFTQALLPRERPVIPQRWQDWAYDVWQDE
QHPKGLWRRTRWTSWRSGHADWQTLIDLDALGEAQGVQWVFDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQCRFVD
DGFSIEAAGKHSVEWIDRDTIYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVVFRGARGDISVDAQYDPLDRHHAIE
QAINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGWLLLQPRLDWTCGGARYAGGSLLAIREDAFVAGERAFAALFEPNE
RTSACGWTHTRRYVLVSWLDDVLTRTMLWLPERQDDGAWRWHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLKPPEY
SLANLASDDLSAWTLLDRWPTQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDNAARAPRPCLLNGYGGFAIALTPDY
DPLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQTQRQRSFDDFIAVAEKLVADGVTSAAQLGIRGGSNGGLLVAACM
IQRPDLFGAVVSDVPLLDMQRYALLHAGASWLDEFGDPDDPAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRVHPAH
ARKMVARMQAQGHRNVWLIEKTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDARE
>Mature_697_residues
PHASWPEQADPHQFLEELDSAASVGWVDAQNARTHDAPWLDEAHYRALVERFTQALLPRERPVIPQRWQDWAYDVWQDEQ
HPKGLWRRTRWTSWRSGHADWQTLIDLDALGEAQGVQWVFDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQCRFVDD
GFSIEAAGKHSVEWIDRDTIYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVVFRGARGDISVDAQYDPLDRHHAIEQ
AINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGWLLLQPRLDWTCGGARYAGGSLLAIREDAFVAGERAFAALFEPNER
TSACGWTHTRRYVLVSWLDDVLTRTMLWLPERQDDGAWRWHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLKPPEYS
LANLASDDLSAWTLLDRWPTQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDNAARAPRPCLLNGYGGFAIALTPDYD
PLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQTQRQRSFDDFIAVAEKLVADGVTSAAQLGIRGGSNGGLLVAACMI
QRPDLFGAVVSDVPLLDMQRYALLHAGASWLDEFGDPDDPAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRVHPAHA
RKMVARMQAQGHRNVWLIEKTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDARE

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=297, Percent_Identity=37.7104377104377, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI108860686, Length=250, Percent_Identity=29.2, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI284172438, Length=250, Percent_Identity=29.2, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI284172431, Length=250, Percent_Identity=29.2, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI284172420, Length=250, Percent_Identity=29.2, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI284172413, Length=250, Percent_Identity=29.2, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI70778815, Length=250, Percent_Identity=29.2, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI108860692, Length=286, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788150, Length=263, Percent_Identity=31.1787072243346, Blast_Score=126, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24583414, Length=300, Percent_Identity=36.3333333333333, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI221510989, Length=233, Percent_Identity=39.9141630901288, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 78154; Mature: 78023

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHASWPEQADPHQFLEELDSAASVGWVDAQNARTHDAPWLDEAHYRALVERFTQALLPR
CCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
ERPVIPQRWQDWAYDVWQDEQHPKGLWRRTRWTSWRSGHADWQTLIDLDALGEAQGVQWV
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEE
FDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQCRFVDDGFSIEAAGKHSVEWIDRDT
ECCEEEECCCCCEEEEEEECCCCCEEEEEECCCEEEEEECCCCEEEECCCCCEEEECCCE
IYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVVFRGARGDISVDAQYDPLDRHHAIE
EEEEECCCCCEEECCCCCHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCCCCHHHHHHH
QAINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGWLLLQPRLDWTCGGARYAGGSLLAI
HHHHHCCCCEEEEECCCCEEEECCCCCEEEEEECCEEEECCCCCEEECCCEECCCEEEEE
REDAFVAGERAFAALFEPNERTSACGWTHTRRYVLVSWLDDVLTRTMLWLPERQDDGAWR
ECCCEECCCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
WHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLKPPEYSLANLASDDLSAWTLLDRWP
ECCCCCCCCCCEEECCCCCCCCCCCEEEEEHHHCCCCCCCHHHHHCCCCCHHEEHHHCCC
TQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDNAARAPRPCLLNGYGGFAIALTPDY
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEECCCCEEEEECCCC
DPLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQTQRQRSFDDFIAVAEKLVADGVTS
CCCEECCEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AAQLGIRGGSNGGLLVAACMIQRPDLFGAVVSDVPLLDMQRYALLHAGASWLDEFGDPDD
HHHHCCCCCCCCCEEEEEEHHCCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCCC
PAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRVHPAHARKMVARMQAQGHRNVWLIE
CHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
KTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDARE
CCCCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCCCCC
>Mature Secondary Structure 
PHASWPEQADPHQFLEELDSAASVGWVDAQNARTHDAPWLDEAHYRALVERFTQALLPR
CCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
ERPVIPQRWQDWAYDVWQDEQHPKGLWRRTRWTSWRSGHADWQTLIDLDALGEAQGVQWV
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEE
FDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQCRFVDDGFSIEAAGKHSVEWIDRDT
ECCEEEECCCCCEEEEEEECCCCCEEEEEECCCEEEEEECCCCEEEECCCCCEEEECCCE
IYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVVFRGARGDISVDAQYDPLDRHHAIE
EEEEECCCCCEEECCCCCHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCCCCHHHHHHH
QAINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGWLLLQPRLDWTCGGARYAGGSLLAI
HHHHHCCCCEEEEECCCCEEEECCCCCEEEEEECCEEEECCCCCEEECCCEECCCEEEEE
REDAFVAGERAFAALFEPNERTSACGWTHTRRYVLVSWLDDVLTRTMLWLPERQDDGAWR
ECCCEECCCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
WHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLKPPEYSLANLASDDLSAWTLLDRWP
ECCCCCCCCCCEEECCCCCCCCCCCEEEEEHHHCCCCCCCHHHHHCCCCCHHEEHHHCCC
TQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDNAARAPRPCLLNGYGGFAIALTPDY
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEECCCCEEEEECCCC
DPLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQTQRQRSFDDFIAVAEKLVADGVTS
CCCEECCEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AAQLGIRGGSNGGLLVAACMIQRPDLFGAVVSDVPLLDMQRYALLHAGASWLDEFGDPDD
HHHHCCCCCCCCCEEEEEEHHCCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCCC
PAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRVHPAHARKMVARMQAQGHRNVWLIE
CHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
KTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDARE
CCCCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]