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The map label for this gene is asnB-1 [H]

Identifier: 126445766

GI number: 126445766

Start: 1483642

End: 1485612

Strand: Reverse

Name: asnB-1 [H]

Synonym: BMA10247_A1535

Alternate gene names: 126445766

Gene position: 1485612-1483642 (Counterclockwise)

Preceding gene: 126446787

Following gene: 126447773

Centisome position: 63.15

GC content: 70.02

Gene sequence:

>1971_bases
ATGTGCGGAATCACCGGCTTCGTGGGCCCTGTCGCATGCGCGCCGCAGACGGCTCGCGCGACCCTCGCGCGAATGACGGA
CAGTCTCGCGCATCGCGGCCCGGACGATACCGGTTTGTGGATCGATCACGACGCGGGCGTCGCGCTCGGCCATCGGCGCC
TGTCGATCGTCGACTTGTCCGAGCACGGGCATCAGCCGATGCTGTCCGCGTGCGGCCGTTATGCGATGACGTTCAACGGC
GAGATCTACAACTTTCGCCAGTTGCGCAGCGAGCTCGAGCGCGAACATCGCGCGCCCGAATGGCGCGGTCATTCGGATAG
CGAAGTGTTGCTCGCGGCGTTCTGCGCGTGGGGCGTCGATCGCGCGTTGCAAAAGGCGGTCGGCATGTTCGCGCTCGCGC
TGTGGAACCGCGAATCGCGCGTGCTCACGCTCGCGCGCGATCGCATCGGCGAGAAGCCGCTCTATTACGGCCGCATCGGC
GGCTCGTTCGTGTTCGGCTCCGAGCTGAAGGCGCTGCGGCGTTTTCCCGGCTTCGACGCGCATCCGATCGACCGCGACGC
GCTGTGCCTGTATCTGCGCCACGCGAGCGTGCCCGCGCCGTACACGATCTACGAAGGCATCCGCAAGCTGCCGCCCGGCA
CGTACGCGCAGCTCGAGGACCTCGCCGAAGCGCCCGCGCCGCGCCGCTACTGGTCGCTCGAGCGCGCGATCGCCGCAGGC
CGCGCGAATCCGTTCGACGGCGACGCGAACGCCGCCGTCGCGCAGCTCGATTACGTGTTGCGCGACGCGATCGCCGAGCA
GTCGGTCGCCGACGTGCCGCTCGGCGCGTTCCTGTCGGGCGGCGTCGATTCGTCGGTGATCGTCGCGCTGATGCAGACGC
AGACCGCGCAGCCGGTGCAGACCTTCACGATCGGCTTTCACGAGGCGGGCTACGACGAGGCCGGCTATGCGAAGGCGGTT
GCGCGGCATTTGGGCACGTCGCACACCGAGCTCTACGTGAAGCCGGCCGACGTGCTCGCGACCGTCCCGCGCATGCCGTG
CGTCTACGACGAGCCGTTCGCCGATTCGTCGCAGATTCCGACGCTGCTCGTTTCCGAGCTCACCCGCCGGCACGTGAAGG
TGAGCCTGTCCGGCGACGGCGGTGACGAGCTGTTCGGCGGCTACACGCGCTATTTCCTGACGCCGCGCCTATGGGGGCGG
CTGCGCCGCGTGCCGGCCGCGGTGCGGATGCGGATCGCGGCCGCGCTGCACGCGCTGCGGCCCGACCATGCGGATCAGAT
CGCCTCGCTCGTGCAGGGCGCGTGGAGCGCGTCCGCGCAGCGCGACGCGTCGCCGCGCGTCGGGGACCGCCTGCACAAGC
TCGCGAACGTGATGACGGCGGACAGCCGCGCGTCGCTGTACCGGTTGCTGATGTCGTCGGTCCATCATCCGGAGCGCTTC
ACGCTCGCGGGGCACGAGCCGCAGACGCTGATCGACACGCCGGGCGCGTGGCCCGCCGGGCTGTCGTTCGCCGAGCAGGC
CATGGCCGTCGATACGCTCACGTATCTGCCGACCGACATCCTGACGAAAGTCGACCGCGCGGCGATGGCGGTGAGCCTCG
AGACGCGGATGCCGTTTCTCGATCACCGCGTCGTCGAGTTCGCGTGGCGGCTGCCGGAGGCGCTGCGCATCCCGGATGGG
CAGGACAAGTGGTTGCTGCGCAAGCTGCTGTATCGCTACGTGCCGCGCCCGCTGATCGACCGGCCGAAACAGGGCTTCTG
CGCGCCCGTCGGCGCGTGGCTGCGCGGCGAATTGAGCGACTGGGCGGCGGAGCTGCTGCGCCCCGCGCGCCTGCGCGACG
AAGGCTACCTCGACGCGGCGCGCGTCGAGCGCCTGTGGCGGCAGCACCAGTCGGGCCGGATGAACTGGCAGCACCCGCTC
TGGACGGTGCTGATGTTCCAGGCATGGCTCGAGCGGCAGCGCGCCGAGTAG

Upstream 100 bases:

>100_bases
GATTAATTAACGAATATTTTCTTCGTAATATGCGCCCAACCGACGGGCGCCGGTCGCCCGTCGAGCGATATCCTTTCACC
ATTCGAACGAGAAATTCGTC

Downstream 100 bases:

>100_bases
GGCGGCCGGCCGCGCGAACGAAGGCTTGCGCGCGGGCGGCGCGCGCGGCGGTCAGTGATATTCGGCGACGCGCTCGAGGT
GCGGCGCGACGGCCGGGCGC

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MCGITGFVGPVACAPQTARATLARMTDSLAHRGPDDTGLWIDHDAGVALGHRRLSIVDLSEHGHQPMLSACGRYAMTFNG
EIYNFRQLRSELEREHRAPEWRGHSDSEVLLAAFCAWGVDRALQKAVGMFALALWNRESRVLTLARDRIGEKPLYYGRIG
GSFVFGSELKALRRFPGFDAHPIDRDALCLYLRHASVPAPYTIYEGIRKLPPGTYAQLEDLAEAPAPRRYWSLERAIAAG
RANPFDGDANAAVAQLDYVLRDAIAEQSVADVPLGAFLSGGVDSSVIVALMQTQTAQPVQTFTIGFHEAGYDEAGYAKAV
ARHLGTSHTELYVKPADVLATVPRMPCVYDEPFADSSQIPTLLVSELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWGR
LRRVPAAVRMRIAAALHALRPDHADQIASLVQGAWSASAQRDASPRVGDRLHKLANVMTADSRASLYRLLMSSVHHPERF
TLAGHEPQTLIDTPGAWPAGLSFAEQAMAVDTLTYLPTDILTKVDRAAMAVSLETRMPFLDHRVVEFAWRLPEALRIPDG
QDKWLLRKLLYRYVPRPLIDRPKQGFCAPVGAWLRGELSDWAAELLRPARLRDEGYLDAARVERLWRQHQSGRMNWQHPL
WTVLMFQAWLERQRAE

Sequences:

>Translated_656_residues
MCGITGFVGPVACAPQTARATLARMTDSLAHRGPDDTGLWIDHDAGVALGHRRLSIVDLSEHGHQPMLSACGRYAMTFNG
EIYNFRQLRSELEREHRAPEWRGHSDSEVLLAAFCAWGVDRALQKAVGMFALALWNRESRVLTLARDRIGEKPLYYGRIG
GSFVFGSELKALRRFPGFDAHPIDRDALCLYLRHASVPAPYTIYEGIRKLPPGTYAQLEDLAEAPAPRRYWSLERAIAAG
RANPFDGDANAAVAQLDYVLRDAIAEQSVADVPLGAFLSGGVDSSVIVALMQTQTAQPVQTFTIGFHEAGYDEAGYAKAV
ARHLGTSHTELYVKPADVLATVPRMPCVYDEPFADSSQIPTLLVSELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWGR
LRRVPAAVRMRIAAALHALRPDHADQIASLVQGAWSASAQRDASPRVGDRLHKLANVMTADSRASLYRLLMSSVHHPERF
TLAGHEPQTLIDTPGAWPAGLSFAEQAMAVDTLTYLPTDILTKVDRAAMAVSLETRMPFLDHRVVEFAWRLPEALRIPDG
QDKWLLRKLLYRYVPRPLIDRPKQGFCAPVGAWLRGELSDWAAELLRPARLRDEGYLDAARVERLWRQHQSGRMNWQHPL
WTVLMFQAWLERQRAE
>Mature_656_residues
MCGITGFVGPVACAPQTARATLARMTDSLAHRGPDDTGLWIDHDAGVALGHRRLSIVDLSEHGHQPMLSACGRYAMTFNG
EIYNFRQLRSELEREHRAPEWRGHSDSEVLLAAFCAWGVDRALQKAVGMFALALWNRESRVLTLARDRIGEKPLYYGRIG
GSFVFGSELKALRRFPGFDAHPIDRDALCLYLRHASVPAPYTIYEGIRKLPPGTYAQLEDLAEAPAPRRYWSLERAIAAG
RANPFDGDANAAVAQLDYVLRDAIAEQSVADVPLGAFLSGGVDSSVIVALMQTQTAQPVQTFTIGFHEAGYDEAGYAKAV
ARHLGTSHTELYVKPADVLATVPRMPCVYDEPFADSSQIPTLLVSELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWGR
LRRVPAAVRMRIAAALHALRPDHADQIASLVQGAWSASAQRDASPRVGDRLHKLANVMTADSRASLYRLLMSSVHHPERF
TLAGHEPQTLIDTPGAWPAGLSFAEQAMAVDTLTYLPTDILTKVDRAAMAVSLETRMPFLDHRVVEFAWRLPEALRIPDG
QDKWLLRKLLYRYVPRPLIDRPKQGFCAPVGAWLRGELSDWAAELLRPARLRDEGYLDAARVERLWRQHQSGRMNWQHPL
WTVLMFQAWLERQRAE

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=420, Percent_Identity=25.2380952380952, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI168229252, Length=420, Percent_Identity=25.2380952380952, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI168229250, Length=420, Percent_Identity=25.2380952380952, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI296010848, Length=393, Percent_Identity=25.4452926208651, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI296010852, Length=325, Percent_Identity=24.3076923076923, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI296010850, Length=325, Percent_Identity=24.3076923076923, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1786889, Length=413, Percent_Identity=29.5399515738499, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71993933, Length=403, Percent_Identity=26.0545905707196, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25147557, Length=403, Percent_Identity=26.0545905707196, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560178, Length=402, Percent_Identity=26.865671641791, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25147560, Length=337, Percent_Identity=25.8160237388724, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=434, Percent_Identity=28.110599078341, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325403, Length=411, Percent_Identity=28.9537712895377, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45553209, Length=417, Percent_Identity=27.8177458033573, Blast_Score=133, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 73173; Mature: 73173

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGFVGPVACAPQTARATLARMTDSLAHRGPDDTGLWIDHDAGVALGHRRLSIVDLS
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEECCCEEEEEECH
EHGHQPMLSACGRYAMTFNGEIYNFRQLRSELEREHRAPEWRGHSDSEVLLAAFCAWGVD
HCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
RALQKAVGMFALALWNRESRVLTLARDRIGEKPLYYGRIGGSFVFGSELKALRRFPGFDA
HHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCEEEECCCCCEEEHHHHHHHHHCCCCCC
HPIDRDALCLYLRHASVPAPYTIYEGIRKLPPGTYAQLEDLAEAPAPRRYWSLERAIAAG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHC
RANPFDGDANAAVAQLDYVLRDAIAEQSVADVPLGAFLSGGVDSSVIVALMQTQTAQPVQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCC
TFTIGFHEAGYDEAGYAKAVARHLGTSHTELYVKPADVLATVPRMPCVYDEPFADSSQIP
EEEEEHHHCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCH
TLLVSELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWGRLRRVPAAVRMRIAAALHALR
HHHHHHHHHCEEEEEECCCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PDHADQIASLVQGAWSASAQRDASPRVGDRLHKLANVMTADSRASLYRLLMSSVHHPERF
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
TLAGHEPQTLIDTPGAWPAGLSFAEQAMAVDTLTYLPTDILTKVDRAAMAVSLETRMPFL
EECCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEHHHCCCHH
DHRVVEFAWRLPEALRIPDGQDKWLLRKLLYRYVPRPLIDRPKQGFCAPVGAWLRGELSD
HHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHCCHHH
WAAELLRPARLRDEGYLDAARVERLWRQHQSGRMNWQHPLWTVLMFQAWLERQRAE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGITGFVGPVACAPQTARATLARMTDSLAHRGPDDTGLWIDHDAGVALGHRRLSIVDLS
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEECCCEEEEEECH
EHGHQPMLSACGRYAMTFNGEIYNFRQLRSELEREHRAPEWRGHSDSEVLLAAFCAWGVD
HCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
RALQKAVGMFALALWNRESRVLTLARDRIGEKPLYYGRIGGSFVFGSELKALRRFPGFDA
HHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCEEEECCCCCEEEHHHHHHHHHCCCCCC
HPIDRDALCLYLRHASVPAPYTIYEGIRKLPPGTYAQLEDLAEAPAPRRYWSLERAIAAG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHC
RANPFDGDANAAVAQLDYVLRDAIAEQSVADVPLGAFLSGGVDSSVIVALMQTQTAQPVQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCC
TFTIGFHEAGYDEAGYAKAVARHLGTSHTELYVKPADVLATVPRMPCVYDEPFADSSQIP
EEEEEHHHCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCH
TLLVSELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWGRLRRVPAAVRMRIAAALHALR
HHHHHHHHHCEEEEEECCCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PDHADQIASLVQGAWSASAQRDASPRVGDRLHKLANVMTADSRASLYRLLMSSVHHPERF
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
TLAGHEPQTLIDTPGAWPAGLSFAEQAMAVDTLTYLPTDILTKVDRAAMAVSLETRMPFL
EECCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEHHHCCCHH
DHRVVEFAWRLPEALRIPDGQDKWLLRKLLYRYVPRPLIDRPKQGFCAPVGAWLRGELSD
HHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHCCHHH
WAAELLRPARLRDEGYLDAARVERLWRQHQSGRMNWQHPLWTVLMFQAWLERQRAE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]