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The map label for this gene is lip2 [H]

Identifier: 126445670

GI number: 126445670

Start: 1877588

End: 1878547

Strand: Reverse

Name: lip2 [H]

Synonym: BMA10247_A1955

Alternate gene names: 126445670

Gene position: 1878547-1877588 (Counterclockwise)

Preceding gene: 126447670

Following gene: 126447429

Centisome position: 79.85

GC content: 73.65

Gene sequence:

>960_bases
ATGCTGGAGCCGACCATCGAGGCGTTCGTCGCGCGCGTCGCCGCGTGGTATCCGGACGATGCGGCGACGCGGCCGATCGC
CGAGCAGCGCGCGCTATACGAGCGTTTCGCCGCGGCATGGACGCCGGCGCTGCCGGCGGATGTCGACACGCGCGATGCGC
GGCTCGGCGCGTTCGGCGACGGCCGCGAGATCCGGCTGCGGCGCTATCGGTCGCGGTGCGCCGCCGCGTGCGGGACCGTG
CTGTTCTTTCACGGCGGCGGCTTCGTCGTCGGCTCGCTCGACAGCCACGCGCTCATGACGGCCCGCATCGCGGCGGATAC
CGGGCTCGACGTGATCGCCGTCGACTACCGGCTCGCTCCCGAGCATCGTGCGCCGGCCGCGTTCGACGATTGTCTGGCGG
TGACCCTGGCCGCGCTCGACGGCCGGCTACCGTTCGAATCGATCGCGCTGCCGCTGCGGCTTGCCGGCGACAGCGCGGGC
GGCATGCTCGCGGCGTCGGTGGCCACGGCGCTGCGCGACGGCGGACGCATCGGGCGTGACGGCGGCGGTCGAGCGGGCGG
AGGCGTTTGCGCGGTCGCGCTCGTTTATCCGATGCTCGGTTTCGAGCCGCAGGCCCCTGCGCGCGATACGCACGCGCATG
CGCCGATGCTCACGCTCGACGATGTCCACGCATATCGCCGCCACTATTGGGGCGATGCCGGGGCGCCGCCCGGCGCGCTG
CCGCTCGACGCGCGGCGCTTCGACGGCTTGCCGCCCGTGCTGGCGATCGGCGCGGAGCACGATCCGTTGCGCGACGACGC
GCGCGTCTATGCCGAGCGCATTCGCTCGGCGGGCGGCGCCGCGCGATTCTGGTGCGGCGACGGGCTCGTGCACGGTTGCT
GGCGCGCGCTCGACACGAGCGCGCAAGCGGCGCGGATGCATCGGGCCGTGTGCGAATTCCTGCTTGCGCCCGACGGATGA

Upstream 100 bases:

>100_bases
TGCAGCGCGCGCATCCCGCGCCGGCGTACGCCGGGCGCGTGATCGGCCTGCCCGCGCGCCGCTGACGCGGCGCGGACCGG
CGCATCGAGAGGGGCACACG

Downstream 100 bases:

>100_bases
TGCGCGCCGCCCGGCCGCCGGTTCGGACGCGACGAAGGGCGGATGTGCCGACGGCTTGGTACGCGCAAGGCGGTCGTCGG
GGCGACAAATGAAAAAATCG

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLEPTIEAFVARVAAWYPDDAATRPIAEQRALYERFAAAWTPALPADVDTRDARLGAFGDGREIRLRRYRSRCAAACGTV
LFFHGGGFVVGSLDSHALMTARIAADTGLDVIAVDYRLAPEHRAPAAFDDCLAVTLAALDGRLPFESIALPLRLAGDSAG
GMLAASVATALRDGGRIGRDGGGRAGGGVCAVALVYPMLGFEPQAPARDTHAHAPMLTLDDVHAYRRHYWGDAGAPPGAL
PLDARRFDGLPPVLAIGAEHDPLRDDARVYAERIRSAGGAARFWCGDGLVHGCWRALDTSAQAARMHRAVCEFLLAPDG

Sequences:

>Translated_319_residues
MLEPTIEAFVARVAAWYPDDAATRPIAEQRALYERFAAAWTPALPADVDTRDARLGAFGDGREIRLRRYRSRCAAACGTV
LFFHGGGFVVGSLDSHALMTARIAADTGLDVIAVDYRLAPEHRAPAAFDDCLAVTLAALDGRLPFESIALPLRLAGDSAG
GMLAASVATALRDGGRIGRDGGGRAGGGVCAVALVYPMLGFEPQAPARDTHAHAPMLTLDDVHAYRRHYWGDAGAPPGAL
PLDARRFDGLPPVLAIGAEHDPLRDDARVYAERIRSAGGAARFWCGDGLVHGCWRALDTSAQAARMHRAVCEFLLAPDG
>Mature_319_residues
MLEPTIEAFVARVAAWYPDDAATRPIAEQRALYERFAAAWTPALPADVDTRDARLGAFGDGREIRLRRYRSRCAAACGTV
LFFHGGGFVVGSLDSHALMTARIAADTGLDVIAVDYRLAPEHRAPAAFDDCLAVTLAALDGRLPFESIALPLRLAGDSAG
GMLAASVATALRDGGRIGRDGGGRAGGGVCAVALVYPMLGFEPQAPARDTHAHAPMLTLDDVHAYRRHYWGDAGAPPGAL
PLDARRFDGLPPVLAIGAEHDPLRDDARVYAERIRSAGGAARFWCGDGLVHGCWRALDTSAQAARMHRAVCEFLLAPDG

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=176, Percent_Identity=30.1136363636364, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=293, Percent_Identity=29.6928327645051, Blast_Score=88, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI72001146, Length=139, Percent_Identity=33.0935251798561, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17567059, Length=205, Percent_Identity=30.7317073170732, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 33862; Mature: 33862

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPTIEAFVARVAAWYPDDAATRPIAEQRALYERFAAAWTPALPADVDTRDARLGAFGD
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GREIRLRRYRSRCAAACGTVLFFHGGGFVVGSLDSHALMTARIAADTGLDVIAVDYRLAP
CHHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECC
EHRAPAAFDDCLAVTLAALDGRLPFESIALPLRLAGDSAGGMLAASVATALRDGGRIGRD
CCCCCCHHHHHHHHHHHHHCCCCCHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
GGGRAGGGVCAVALVYPMLGFEPQAPARDTHAHAPMLTLDDVHAYRRHYWGDAGAPPGAL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCC
PLDARRFDGLPPVLAIGAEHDPLRDDARVYAERIRSAGGAARFWCGDGLVHGCWRALDTS
CCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCH
AQAARMHRAVCEFLLAPDG
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLEPTIEAFVARVAAWYPDDAATRPIAEQRALYERFAAAWTPALPADVDTRDARLGAFGD
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GREIRLRRYRSRCAAACGTVLFFHGGGFVVGSLDSHALMTARIAADTGLDVIAVDYRLAP
CHHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECC
EHRAPAAFDDCLAVTLAALDGRLPFESIALPLRLAGDSAGGMLAASVATALRDGGRIGRD
CCCCCCHHHHHHHHHHHHHCCCCCHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
GGGRAGGGVCAVALVYPMLGFEPQAPARDTHAHAPMLTLDDVHAYRRHYWGDAGAPPGAL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCC
PLDARRFDGLPPVLAIGAEHDPLRDDARVYAERIRSAGGAARFWCGDGLVHGCWRALDTS
CCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCH
AQAARMHRAVCEFLLAPDG
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]