| Definition | Burkholderia mallei NCTC 10247 chromosome II, complete genome. |
|---|---|
| Accession | NC_009079 |
| Length | 2,352,693 |
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The map label for this gene is hdhA [C]
Identifier: 126445594
GI number: 126445594
Start: 244040
End: 244810
Strand: Direct
Name: hdhA [C]
Synonym: BMA10247_A0281
Alternate gene names: 126445594
Gene position: 244040-244810 (Clockwise)
Preceding gene: 126446247
Following gene: 126446626
Centisome position: 10.37
GC content: 67.7
Gene sequence:
>771_bases ATGAGCGCACCCAACCTGTTCGACCTGTCCGGCAAGATCGCCGTCGTCACCGGCGGCAGCCGCGGCATCGGCGCGGAAGT CGCGAAGCTGCTCGGCGCGTTCGGCGCGCATGTCGTCGTCACGAGCCGGCGCCTCGACGATTGCCGCGCGATCGCGGACG AGATCGTCGCGGCGGGCGGCTCGGCCGAGGCGGCCGCGTGCCATATCGGCGAGCTCGAGCAGATCGACGCGCTGTACGCG TCACTCGAAGGCCGGCATGGGCGGCTCGACGTGCTCGTCAACAACGCGGCGACCAATCCGTACTACGGGCCGATCGTCGA TACGGATCTCGGCGCGTTCCAGAAGACCGTCGACGTGAACATCCGCGGCTACTTCTTCATGTCGAGCCGCGGCGCGAAGC TGATGGGCAAGAACGGCGGCGGATCGATCGTCAACGTCGCATCGGTGAACGGCGTGATTCCCGGCTACTGGCAGGGCATC TACTCGATCACGAAGGCGGCGGTGATCTCGATGACCAAGGCGTTCGCGATCGAATGCGCGGCAAGCGGCGTGCGCTGCAA CGCGCTGCTGCCGGGGCTGACCGACACGAAATTCGCGTCGGCGCTCACGCAGACGCCCGAGGTGCTGAAGCAGGTGCTCG CGCACGTGCCGATGCGGCGCGCCGCGCAGCCGACGGAGATGGCGGGCGCGGTGCTGTATCTGGCGTCGGCGGCGTCGAGC TATACGACGGGGACGGTGCTGAACGTCGACGGCGGGTATTTGTCGGTTTGA
Upstream 100 bases:
>100_bases GACGACGTACAACGGCTGGCCGCACCTCGACGGCGAGCGCGACGAACGGCTCGTCACGCACCGCTGATTCCTTTTCCTTC ACCCACTCGGGATATCCTTC
Downstream 100 bases:
>100_bases CGGCGATAGGGTGGGCGACGAGCCACGAACTGCGAGCTGTGAGCTGCGAGTTGCGGGTTGCGAGCGCCGGATACCCGACG CTCGAAGTCTAGACGCCTGA
Product: short chain dehydrogenase
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: NA
Number of amino acids: Translated: 256; Mature: 255
Protein sequence:
>256_residues MSAPNLFDLSGKIAVVTGGSRGIGAEVAKLLGAFGAHVVVTSRRLDDCRAIADEIVAAGGSAEAAACHIGELEQIDALYA SLEGRHGRLDVLVNNAATNPYYGPIVDTDLGAFQKTVDVNIRGYFFMSSRGAKLMGKNGGGSIVNVASVNGVIPGYWQGI YSITKAAVISMTKAFAIECAASGVRCNALLPGLTDTKFASALTQTPEVLKQVLAHVPMRRAAQPTEMAGAVLYLASAASS YTTGTVLNVDGGYLSV
Sequences:
>Translated_256_residues MSAPNLFDLSGKIAVVTGGSRGIGAEVAKLLGAFGAHVVVTSRRLDDCRAIADEIVAAGGSAEAAACHIGELEQIDALYA SLEGRHGRLDVLVNNAATNPYYGPIVDTDLGAFQKTVDVNIRGYFFMSSRGAKLMGKNGGGSIVNVASVNGVIPGYWQGI YSITKAAVISMTKAFAIECAASGVRCNALLPGLTDTKFASALTQTPEVLKQVLAHVPMRRAAQPTEMAGAVLYLASAASS YTTGTVLNVDGGYLSV >Mature_255_residues SAPNLFDLSGKIAVVTGGSRGIGAEVAKLLGAFGAHVVVTSRRLDDCRAIADEIVAAGGSAEAAACHIGELEQIDALYAS LEGRHGRLDVLVNNAATNPYYGPIVDTDLGAFQKTVDVNIRGYFFMSSRGAKLMGKNGGGSIVNVASVNGVIPGYWQGIY SITKAAVISMTKAFAIECAASGVRCNALLPGLTDTKFASALTQTPEVLKQVLAHVPMRRAAQPTEMAGAVLYLASAASSY TTGTVLNVDGGYLSV
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=247, Percent_Identity=36.0323886639676, Blast_Score=159, Evalue=3e-39, Organism=Homo sapiens, GI126723750, Length=249, Percent_Identity=30.9236947791165, Blast_Score=122, Evalue=3e-28, Organism=Homo sapiens, GI5031737, Length=243, Percent_Identity=29.6296296296296, Blast_Score=120, Evalue=2e-27, Organism=Homo sapiens, GI19923817, Length=261, Percent_Identity=30.6513409961686, Blast_Score=117, Evalue=1e-26, Organism=Homo sapiens, GI126723191, Length=193, Percent_Identity=34.1968911917098, Blast_Score=115, Evalue=4e-26, Organism=Homo sapiens, GI40254992, Length=241, Percent_Identity=33.195020746888, Blast_Score=113, Evalue=2e-25, Organism=Homo sapiens, GI209862901, Length=246, Percent_Identity=34.9593495934959, Blast_Score=102, Evalue=3e-22, Organism=Homo sapiens, GI19923983, Length=246, Percent_Identity=34.9593495934959, Blast_Score=102, Evalue=3e-22, Organism=Homo sapiens, GI10190704, Length=253, Percent_Identity=32.0158102766798, Blast_Score=102, Evalue=5e-22, Organism=Homo sapiens, GI7705925, Length=250, Percent_Identity=31.6, Blast_Score=101, Evalue=5e-22, Organism=Homo sapiens, GI33667109, Length=191, Percent_Identity=30.8900523560209, Blast_Score=101, Evalue=6e-22, Organism=Homo sapiens, GI15277342, Length=268, Percent_Identity=30.2238805970149, Blast_Score=92, Evalue=6e-19, Organism=Homo sapiens, GI4503301, Length=261, Percent_Identity=28.3524904214559, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI66933014, Length=186, Percent_Identity=32.7956989247312, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI59889578, Length=223, Percent_Identity=30.4932735426009, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI31542939, Length=204, Percent_Identity=28.921568627451, Blast_Score=85, Evalue=5e-17, Organism=Homo sapiens, GI109715829, Length=256, Percent_Identity=26.5625, Blast_Score=81, Evalue=1e-15, Organism=Homo sapiens, GI302344755, Length=116, Percent_Identity=35.3448275862069, Blast_Score=78, Evalue=7e-15, Organism=Homo sapiens, GI4758504, Length=256, Percent_Identity=31.640625, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI4504505, Length=267, Percent_Identity=28.0898876404494, Blast_Score=71, Evalue=8e-13, Organism=Homo sapiens, GI224922801, Length=179, Percent_Identity=28.4916201117318, Blast_Score=71, Evalue=1e-12, Organism=Homo sapiens, GI32455239, Length=194, Percent_Identity=31.9587628865979, Blast_Score=68, Evalue=9e-12, Organism=Homo sapiens, GI5031765, Length=194, Percent_Identity=31.9587628865979, Blast_Score=68, Evalue=9e-12, Organism=Homo sapiens, GI225579078, Length=213, Percent_Identity=26.2910798122066, Blast_Score=67, Evalue=1e-11, Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=34.5381526104418, Blast_Score=130, Evalue=8e-32, Organism=Escherichia coli, GI87082160, Length=258, Percent_Identity=31.7829457364341, Blast_Score=128, Evalue=4e-31, Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=33.203125, Blast_Score=121, Evalue=5e-29, Organism=Escherichia coli, GI2367175, Length=246, Percent_Identity=32.9268292682927, Blast_Score=113, Evalue=1e-26, Organism=Escherichia coli, GI1787335, Length=252, Percent_Identity=30.952380952381, Blast_Score=113, Evalue=1e-26, Organism=Escherichia coli, GI1790717, Length=250, Percent_Identity=28, Blast_Score=111, Evalue=4e-26, Organism=Escherichia coli, GI1788459, Length=245, Percent_Identity=32.2448979591837, Blast_Score=108, Evalue=3e-25, Organism=Escherichia coli, GI1789208, Length=255, Percent_Identity=29.8039215686275, Blast_Score=104, Evalue=4e-24, Organism=Escherichia coli, GI1789378, Length=246, Percent_Identity=29.6747967479675, Blast_Score=94, Evalue=8e-21, Organism=Escherichia coli, GI1789057, Length=256, Percent_Identity=29.6875, Blast_Score=89, Evalue=3e-19, Organism=Escherichia coli, GI1786812, Length=255, Percent_Identity=31.3725490196078, Blast_Score=86, Evalue=2e-18, Organism=Escherichia coli, GI2367365, Length=247, Percent_Identity=28.3400809716599, Blast_Score=81, Evalue=5e-17, Organism=Escherichia coli, GI1787820, Length=189, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=8e-12, Organism=Caenorhabditis elegans, GI17560676, Length=256, Percent_Identity=32.421875, Blast_Score=140, Evalue=5e-34, Organism=Caenorhabditis elegans, GI17555706, Length=245, Percent_Identity=35.9183673469388, Blast_Score=120, Evalue=5e-28, Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=35.0806451612903, Blast_Score=115, Evalue=3e-26, Organism=Caenorhabditis elegans, GI17561402, Length=252, Percent_Identity=30.1587301587302, Blast_Score=109, Evalue=1e-24, Organism=Caenorhabditis elegans, GI71994604, Length=247, Percent_Identity=31.9838056680162, Blast_Score=108, Evalue=2e-24, Organism=Caenorhabditis elegans, GI17562910, Length=261, Percent_Identity=31.8007662835249, Blast_Score=107, Evalue=7e-24, Organism=Caenorhabditis elegans, GI193204405, Length=262, Percent_Identity=32.824427480916, Blast_Score=107, Evalue=8e-24, Organism=Caenorhabditis elegans, GI71994600, Length=248, Percent_Identity=31.8548387096774, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI17562906, Length=262, Percent_Identity=31.2977099236641, Blast_Score=103, Evalue=9e-23, Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=31.4393939393939, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17531453, Length=260, Percent_Identity=31.5384615384615, Blast_Score=101, Evalue=3e-22, Organism=Caenorhabditis elegans, GI115534694, Length=253, Percent_Identity=29.6442687747036, Blast_Score=100, Evalue=9e-22, Organism=Caenorhabditis elegans, GI17560220, Length=259, Percent_Identity=31.2741312741313, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17559104, Length=258, Percent_Identity=30.6201550387597, Blast_Score=98, Evalue=5e-21, Organism=Caenorhabditis elegans, GI17538182, Length=264, Percent_Identity=32.1969696969697, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17560150, Length=258, Percent_Identity=31.0077519379845, Blast_Score=94, Evalue=6e-20, Organism=Caenorhabditis elegans, GI17536025, Length=262, Percent_Identity=27.8625954198473, Blast_Score=94, Evalue=7e-20, Organism=Caenorhabditis elegans, GI72000259, Length=260, Percent_Identity=29.2307692307692, Blast_Score=93, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17536651, Length=267, Percent_Identity=28.4644194756554, Blast_Score=92, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17560332, Length=261, Percent_Identity=29.1187739463602, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17562904, Length=264, Percent_Identity=29.9242424242424, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17561272, Length=263, Percent_Identity=28.5171102661597, Blast_Score=88, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17562990, Length=258, Percent_Identity=30.2325581395349, Blast_Score=87, Evalue=6e-18, Organism=Caenorhabditis elegans, GI17555612, Length=211, Percent_Identity=32.2274881516588, Blast_Score=87, Evalue=8e-18, Organism=Caenorhabditis elegans, GI17563726, Length=257, Percent_Identity=26.0700389105058, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI17538480, Length=260, Percent_Identity=28.0769230769231, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=26.2172284644195, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI17551412, Length=265, Percent_Identity=28.6792452830189, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17508651, Length=245, Percent_Identity=26.9387755102041, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=28.996282527881, Blast_Score=76, Evalue=1e-14, Organism=Caenorhabditis elegans, GI17565030, Length=261, Percent_Identity=27.5862068965517, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI71997099, Length=209, Percent_Identity=30.1435406698565, Blast_Score=75, Evalue=2e-14, Organism=Caenorhabditis elegans, GI86565303, Length=220, Percent_Identity=25.4545454545455, Blast_Score=64, Evalue=1e-10, Organism=Saccharomyces cerevisiae, GI6324126, Length=253, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=1e-17, Organism=Saccharomyces cerevisiae, GI6322067, Length=198, Percent_Identity=32.3232323232323, Blast_Score=68, Evalue=2e-12, Organism=Drosophila melanogaster, GI21355319, Length=244, Percent_Identity=35.2459016393443, Blast_Score=152, Evalue=3e-37, Organism=Drosophila melanogaster, GI21357041, Length=248, Percent_Identity=35.4838709677419, Blast_Score=127, Evalue=5e-30, Organism=Drosophila melanogaster, GI28571526, Length=251, Percent_Identity=36.6533864541833, Blast_Score=119, Evalue=3e-27, Organism=Drosophila melanogaster, GI23397609, Length=250, Percent_Identity=32.8, Blast_Score=117, Evalue=1e-26, Organism=Drosophila melanogaster, GI24644339, Length=253, Percent_Identity=32.0158102766798, Blast_Score=113, Evalue=1e-25, Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=32.1285140562249, Blast_Score=103, Evalue=1e-22, Organism=Drosophila melanogaster, GI24644337, Length=251, Percent_Identity=30.6772908366534, Blast_Score=97, Evalue=1e-20, Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=31.7269076305221, Blast_Score=94, Evalue=6e-20, Organism=Drosophila melanogaster, GI17737361, Length=256, Percent_Identity=29.296875, Blast_Score=81, Evalue=7e-16, Organism=Drosophila melanogaster, GI116007236, Length=197, Percent_Identity=27.4111675126904, Blast_Score=79, Evalue=2e-15, Organism=Drosophila melanogaster, GI24641232, Length=238, Percent_Identity=27.7310924369748, Blast_Score=74, Evalue=1e-13, Organism=Drosophila melanogaster, GI116007234, Length=235, Percent_Identity=28.936170212766, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI116007238, Length=175, Percent_Identity=27.4285714285714, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI24665243, Length=206, Percent_Identity=30.5825242718447, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI18859837, Length=238, Percent_Identity=29.8319327731092, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI221331218, Length=206, Percent_Identity=30.5825242718447, Blast_Score=69, Evalue=2e-12, Organism=Drosophila melanogaster, GI21357673, Length=200, Percent_Identity=26, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI161076371, Length=262, Percent_Identity=27.0992366412214, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI24665247, Length=206, Percent_Identity=30.5825242718447, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI221331216, Length=206, Percent_Identity=30.5825242718447, Blast_Score=67, Evalue=9e-12, Organism=Drosophila melanogaster, GI19921754, Length=222, Percent_Identity=27.4774774774775, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI24656154, Length=236, Percent_Identity=27.1186440677966, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI19922628, Length=236, Percent_Identity=27.1186440677966, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI24640492, Length=221, Percent_Identity=24.8868778280543, Blast_Score=65, Evalue=4e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 26430; Mature: 26298
Theoretical pI: Translated: 7.45; Mature: 7.45
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSAPNLFDLSGKIAVVTGGSRGIGAEVAKLLGAFGAHVVVTSRRLDDCRAIADEIVAAGG CCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCC SAEAAACHIGELEQIDALYASLEGRHGRLDVLVNNAATNPYYGPIVDTDLGAFQKTVDVN CCCHHEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEECCCHHHHHHEEEEE IRGYFFMSSRGAKLMGKNGGGSIVNVASVNGVIPGYWQGIYSITKAAVISMTKAFAIECA EEEEEEECCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH ASGVRCNALLPGLTDTKFASALTQTPEVLKQVLAHVPMRRAAQPTEMAGAVLYLASAASS CCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCC YTTGTVLNVDGGYLSV CCCCEEEEECCCEECC >Mature Secondary Structure SAPNLFDLSGKIAVVTGGSRGIGAEVAKLLGAFGAHVVVTSRRLDDCRAIADEIVAAGG CCCCEEECCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCC SAEAAACHIGELEQIDALYASLEGRHGRLDVLVNNAATNPYYGPIVDTDLGAFQKTVDVN CCCHHEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEECCCHHHHHHEEEEE IRGYFFMSSRGAKLMGKNGGGSIVNVASVNGVIPGYWQGIYSITKAAVISMTKAFAIECA EEEEEEECCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH ASGVRCNALLPGLTDTKFASALTQTPEVLKQVLAHVPMRRAAQPTEMAGAVLYLASAASS CCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCC YTTGTVLNVDGGYLSV CCCCEEEEECCCEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 10360571 [H]