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The map label for this gene is mdeA [H]

Identifier: 126445086

GI number: 126445086

Start: 322377

End: 324425

Strand: Reverse

Name: mdeA [H]

Synonym: BURPS668_A0354

Alternate gene names: 126445086

Gene position: 324425-322377 (Counterclockwise)

Preceding gene: 126443579

Following gene: 126444712

Centisome position: 10.37

GC content: 68.96

Gene sequence:

>2049_bases
ATGACCGAACCCGCATCCGCCGACTGGCGCCTCGAGACCCTCGCCGTGCACGGCGGCTATCGCCCCGATCCGACGACGCG
CGCCGCCGCGGTGCCGATCTATCAGACGGTCGCGTTCGCGTTCGACGACACGCAGCACGGCGCGGACCTGTTCGATCTGA
AGGTCCAGGGCAACATCTATTCGCGGATCATGAATCCGACCAACGACGTGCTCGAGCAGCGCGTCGCGGCGCTCGAAGGC
GGCATCGGCGCGCTCGCGCTCGCATCGGGCCAGGCGGCCGTCACTTATTCGATCCTGACGATCGCGGAGGCGGGCGACAA
CATCGTATCGTCGAGCACGCTGTACGGCGGCACCTACAACCTGTTCGCGCATACGCTGCCGCAATACGGAATCTCGACGC
GCTTCGCCGACCCGCGCGAGCCGGCCGCGTTCGACGCGCTGATCGACGCACGGACGAAGGCGATCTTCGCGGAATCGGTC
GGCAATCCGCTCGGCAACATCACCGATATCGCCGCGCTCGCCGAGATCGCGCACCGCCACGGCCTGCCGCTCATCGTCGA
CAACACGGTGCCGACGCCCTACCTGCTGCGCCCGTTCGAGCATGGCGCGGATATCGTCGTCCATTCGCTGACGAAGTATC
TCGGCGGGCACGGCACGAGCCTCGGCGGCGCGATCGTCGATTCGGGCAAGTTTCCGTGGGCGAAGCACGCCGAGCGCTTC
CGGCGGCTGAACGAGCCGGACGTCAGCTATCACGGCGTCGTCTACACCGACGCGTTCGGCGCAGCGGCGTACATCGGCCG
CGCGCGCGTCGTTCCGCTGCGCAACACGGGCGCGGCGCTTTCGCCGTTCAACGCATTCCAGATCCTGCAAGGCATCGAGA
CGCTCGCGCTGCGCATCGAGCGCATCGGCGAGAACGCGCTGAAGATCGCGCAGCATCTCGCGCGGCACGAAAAAGTCGAA
TGGGTCGATTATTCCGGGCTGCCGAATCATCGGGATCACGCGCTCGTCGAGCGCTACCTGTCGGGCCGCGCGCCGGGCAT
CCTGACATTCGGCGTGAAGGGCGGGCGCGCGGCGGGCGCCGCGTTCCAGGACGCTGAAGCTCTTCACGCGCCTCGTCAAC
ATCGGCGACGCGAAGTCGCTCGCGACGCATCCCGCTTCGACGACGCATCGCCAGCTCTCGCCCGACGAGCTCGCGAAAGC
GGGCGTGAAGGAAGAGACGGTGCGGCTGTCGATCGGCATCGAGCACATCGACGATCTGCTCGCCGATCTCGATCAGGCGC
TCGCGAAGGTCTGAAAGGCGGCGCGATCGGACGGGCGCGCGATCGTGCGCTCGTGCGCTCGTGCGCTCGTCCGATCGATC
GTACGGTCCGTCGCGATGCAGCTCGGTGCAACGCGTACGTCGCACCGCATTGCCGCGCGATGCGGCTCGGTGCGTCCGCC
GCTTTGCATCGCCGCGAAGCCGCCGCTTCGCGTCGCGCCGCGATGCGCGCTTCGCGCGGCGTTCAGTCCACCGCATCCAC
CGCATCGCCCGGCATGCGTCGTGCGTTGCGCATCATGTGGCGCGCCGCGGCTCGAAGCCCCACGACGCGAACCTCCGGCT
GGAGGCATGCTTCGCAGTCGAAGCCGTCGCGCCGAGGCCCGCCGTCAAGGCCGGCCTCGGCGTTTCGTCGTGGGGCGCCG
TCTCGGGCGCCACGTCGGGCGCCGCCTTGGATCTCGTCTTCGGCATGGCCTTCAGGCCCCGCGTCGAGCCATGCGATGGT
CGGCTATTCGCCGAGCCTTTCGTCGAGCCTCGCCTCAAGCCCGCCTCCGCCCTGTCACCCGCCTTCGCACCACCGTCAGC
CGGCCTGCAGCCTGAACGCCTGCCGATACCGCTTCGGCGTCGTACCGACGATCCGCACGAAGCGCTCGCGAAACGCGCTC
GCCAAGCCGAACCCGGCTTCGGTCGCGATCCGCTCGACGGACAGCGCCGTCGTCTGCGGCAATGGCTGCGCGCGCCGCAC
GCGCGCGGGCCGAAGCCACTGCAACGGCAAGGTGCCGGTCCGTTCCTGA

Upstream 100 bases:

>100_bases
TCACCAAACAAGCTAAAAGCGCGCGGGCGCGACTCCTATAATCGGCCGACACCTCGACACGAGCAAATCGACCCACGAGC
AAATCGACCGGAGACCCATC

Downstream 100 bases:

>100_bases
AAGCGGCGCGCCAGCGTGCGAACGCTCATCGCGCTCTGCCGGGCGATCGCATCGCGCGTCGACGGCTCGCGCAAATGGCC
GTGCAGCCATTCCATCGATG

Product: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase family protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MTEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTNDVLEQRVAALEG
GIGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYNLFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESV
GNPLGNITDIAALAEIAHRHGLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERF
RRLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIERIGENALKIAQHLARHEKVE
WVDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGAAFQDAEALHAPRQHRRREVARDASRFDDASPALARRARES
GREGRDGAAVDRHRAHRRSARRSRSGAREGLKGGAIGRARDRALVRSCARPIDRTVRRDAARCNAYVAPHCRAMRLGASA
ALHRREAAASRRAAMRASRGVQSTASTASPGMRRALRIMWRAAARSPTTRTSGWRHASQSKPSRRGPPSRPASAFRRGAP
SRAPRRAPPWISSSAWPSGPASSHAMVGYSPSLSSSLASSPPPPCHPPSHHRQPACSLNACRYRFGVVPTIRTKRSRNAL
AKPNPASVAIRSTDSAVVCGNGCARRTRAGRSHCNGKVPVRS

Sequences:

>Translated_682_residues
MTEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTNDVLEQRVAALEG
GIGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYNLFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESV
GNPLGNITDIAALAEIAHRHGLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERF
RRLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIERIGENALKIAQHLARHEKVE
WVDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGAAFQDAEALHAPRQHRRREVARDASRFDDASPALARRARES
GREGRDGAAVDRHRAHRRSARRSRSGAREGLKGGAIGRARDRALVRSCARPIDRTVRRDAARCNAYVAPHCRAMRLGASA
ALHRREAAASRRAAMRASRGVQSTASTASPGMRRALRIMWRAAARSPTTRTSGWRHASQSKPSRRGPPSRPASAFRRGAP
SRAPRRAPPWISSSAWPSGPASSHAMVGYSPSLSSSLASSPPPPCHPPSHHRQPACSLNACRYRFGVVPTIRTKRSRNAL
AKPNPASVAIRSTDSAVVCGNGCARRTRAGRSHCNGKVPVRS
>Mature_681_residues
TEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTNDVLEQRVAALEGG
IGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYNLFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESVG
NPLGNITDIAALAEIAHRHGLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERFR
RLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIERIGENALKIAQHLARHEKVEW
VDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGAAFQDAEALHAPRQHRRREVARDASRFDDASPALARRARESG
REGRDGAAVDRHRAHRRSARRSRSGAREGLKGGAIGRARDRALVRSCARPIDRTVRRDAARCNAYVAPHCRAMRLGASAA
LHRREAAASRRAAMRASRGVQSTASTASPGMRRALRIMWRAAARSPTTRTSGWRHASQSKPSRRGPPSRPASAFRRGAPS
RAPRRAPPWISSSAWPSGPASSHAMVGYSPSLSSSLASSPPPPCHPPSHHRQPACSLNACRYRFGVVPTIRTKRSRNALA
KPNPASVAIRSTDSAVVCGNGCARRTRAGRSHCNGKVPVRS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=353, Percent_Identity=33.4277620396601, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI299473759, Length=353, Percent_Identity=29.1784702549575, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI25453487, Length=344, Percent_Identity=29.3604651162791, Blast_Score=110, Evalue=6e-24,
Organism=Escherichia coli, GI1790375, Length=342, Percent_Identity=28.6549707602339, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1789383, Length=351, Percent_Identity=26.2108262108262, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71980708, Length=342, Percent_Identity=29.2397660818713, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17538055, Length=313, Percent_Identity=32.5878594249201, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17560056, Length=310, Percent_Identity=33.5483870967742, Blast_Score=152, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6323333, Length=346, Percent_Identity=48.8439306358381, Blast_Score=310, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6319307, Length=309, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=206, Percent_Identity=33.495145631068, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321254, Length=364, Percent_Identity=25.8241758241758, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321904, Length=315, Percent_Identity=27.3015873015873, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922554, Length=358, Percent_Identity=33.5195530726257, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 73461; Mature: 73330

Theoretical pI: Translated: 11.59; Mature: 11.59

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIY
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCHHH
SRIMNPTNDVLEQRVAALEGGIGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYN
HHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEHHHCCCCHHCCCEECCCHHH
LFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESVGNPLGNITDIAALAEIAHRH
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
GLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERF
CCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHH
RRLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIE
HHCCCCCCCCCCEEEECCHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RIGENALKIAQHLARHEKVEWVDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGA
HHCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCC
AFQDAEALHAPRQHRRREVARDASRFDDASPALARRARESGREGRDGAAVDRHRAHRRSA
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RRSRSGAREGLKGGAIGRARDRALVRSCARPIDRTVRRDAARCNAYVAPHCRAMRLGASA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHCHHH
ALHRREAAASRRAAMRASRGVQSTASTASPGMRRALRIMWRAAARSPTTRTSGWRHASQS
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KPSRRGPPSRPASAFRRGAPSRAPRRAPPWISSSAWPSGPASSHAMVGYSPSLSSSLASS
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHCC
PPPPCHPPSHHRQPACSLNACRYRFGVVPTIRTKRSRNALAKPNPASVAIRSTDSAVVCG
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCCCCCCCCCCCCCEEEEECCCCEEEEC
NGCARRTRAGRSHCNGKVPVRS
CCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TEPASADWRLETLAVHGGYRPDPTTRAAAVPIYQTVAFAFDDTQHGADLFDLKVQGNIY
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCHHH
SRIMNPTNDVLEQRVAALEGGIGALALASGQAAVTYSILTIAEAGDNIVSSSTLYGGTYN
HHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEHHHCCCCHHCCCEECCCHHH
LFAHTLPQYGISTRFADPREPAAFDALIDARTKAIFAESVGNPLGNITDIAALAEIAHRH
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
GLPLIVDNTVPTPYLLRPFEHGADIVVHSLTKYLGGHGTSLGGAIVDSGKFPWAKHAERF
CCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHH
RRLNEPDVSYHGVVYTDAFGAAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLALRIE
HHCCCCCCCCCCEEEECCHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RIGENALKIAQHLARHEKVEWVDYSGLPNHRDHALVERYLSGRAPGILTFGVKGGRAAGA
HHCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCC
AFQDAEALHAPRQHRRREVARDASRFDDASPALARRARESGREGRDGAAVDRHRAHRRSA
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RRSRSGAREGLKGGAIGRARDRALVRSCARPIDRTVRRDAARCNAYVAPHCRAMRLGASA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHCHHH
ALHRREAAASRRAAMRASRGVQSTASTASPGMRRALRIMWRAAARSPTTRTSGWRHASQS
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KPSRRGPPSRPASAFRRGAPSRAPRRAPPWISSSAWPSGPASSHAMVGYSPSLSSSLASS
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHCC
PPPPCHPPSHHRQPACSLNACRYRFGVVPTIRTKRSRNALAKPNPASVAIRSTDSAVVCG
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCCCCCCCCCCCCCEEEEECCCCEEEEC
NGCARRTRAGRSHCNGKVPVRS
CCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]