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The map label for this gene is yafC [H]

Identifier: 126445047

GI number: 126445047

Start: 385532

End: 386563

Strand: Reverse

Name: yafC [H]

Synonym: BURPS668_A0420

Alternate gene names: 126445047

Gene position: 386563-385532 (Counterclockwise)

Preceding gene: 126444947

Following gene: 126443752

Centisome position: 12.36

GC content: 65.41

Gene sequence:

>1032_bases
ATGATGGGCATCCATGAATCCGGTAACGATGCCGCGCAGTGTGCCCGCTTTACTTTTGCTCAAAAAGTAGCCTATAAACG
AAATTATTTTGATTTCAAATCAACAATGAAGGTCACGCTAGACGAACTTCAGGCCTTCGCGGCCGTGGTCGACACGGGTT
CGATCACCGCGGCCGCGCAACAGCTCGGCCTCACCGTGTCGGCGACGAGCCGCACGCTCGCGCGGCTCGAGGGCAAGCTC
AAGACCACGCTGCTGCGCCGGACCACGCGCCGCCTCGAGCTGACCGAGGAGGGCCGGACGTTCCTCAACAGCGCGCGGGC
AATCATCGATTCGGTCGAAAGCGCGGAAGAGCAGATGCTCGCGCGGCGCGAGAAGCCGTCCGGCCGGCTGCGGGTCGACG
CCGCGTCGCCGTTCATGCTGCATGTGATCGTGCCGCTCGTGCGCGGCTATCGGGAGCGCTTCCCGCGCGTGGAGCTGGAG
CTGAACAGTAACGAGGGCGTCATCGATCTGCTCGAGCGGCGCACCGACGTCGCGATCCGGATCGGCCGCCTGAAGGATTC
GACGCTGCATAGCCGGCTCATCGGCAATAGCCGGCTGCGCATCCTCGCGAGCCCCGCGTATCTCGACGCGCACGGCCAGC
CGCGCAAGGCCGGCGATCTCGGCAAGCATGCGCTGCTCGGCTTCAATCAGCCGGAATCGCTGAACGTGTGGCCGATCCTC
GGCGCGGACGGCGAGCCTTGCCGGATCGAGCCGGCCGTGTGGTCGTCGAGCGGCGAGACGCTCAGACAGCTCGCGCTCGA
CGGCGCGGGCATCGTCTGCCTGTCGGATTTCATGACCGCGCAGGATCGCGAAGCGGGCCGCCTCGTGCAGATCCTCGCGC
GCCACACGCAAGACGTGCGGCAGCCGATTCATGCGGTCTATTACCGGAACACGGCGATTTCGTCGCGAATCGCGTCATTC
GTCGATTATCTGGTCGACGCGCTCGGCGGCGGGAATGCCGCGCAAAAGGCGGCGGCATGGACGCGTCCGTGA

Upstream 100 bases:

>100_bases
GCCGAGGTCGGACGCGATCGTCGGGATCAGCCCGACGATCACGAATTCGGTCGTGCCGATGGCGAAGGCGCTGATCGTCA
GCGCGAGTAGAGCGAGTGGC

Downstream 100 bases:

>100_bases
CGCGCGCGCGATGCCTCACGACGACGGCGCTCGGGAGGAAACTCACTGAGCGACGCGCGCCGGCCGGCCTCCATCACGAA
CGCGCAACGGACGCCGTCGC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MMGIHESGNDAAQCARFTFAQKVAYKRNYFDFKSTMKVTLDELQAFAAVVDTGSITAAAQQLGLTVSATSRTLARLEGKL
KTTLLRRTTRRLELTEEGRTFLNSARAIIDSVESAEEQMLARREKPSGRLRVDAASPFMLHVIVPLVRGYRERFPRVELE
LNSNEGVIDLLERRTDVAIRIGRLKDSTLHSRLIGNSRLRILASPAYLDAHGQPRKAGDLGKHALLGFNQPESLNVWPIL
GADGEPCRIEPAVWSSSGETLRQLALDGAGIVCLSDFMTAQDREAGRLVQILARHTQDVRQPIHAVYYRNTAISSRIASF
VDYLVDALGGGNAAQKAAAWTRP

Sequences:

>Translated_343_residues
MMGIHESGNDAAQCARFTFAQKVAYKRNYFDFKSTMKVTLDELQAFAAVVDTGSITAAAQQLGLTVSATSRTLARLEGKL
KTTLLRRTTRRLELTEEGRTFLNSARAIIDSVESAEEQMLARREKPSGRLRVDAASPFMLHVIVPLVRGYRERFPRVELE
LNSNEGVIDLLERRTDVAIRIGRLKDSTLHSRLIGNSRLRILASPAYLDAHGQPRKAGDLGKHALLGFNQPESLNVWPIL
GADGEPCRIEPAVWSSSGETLRQLALDGAGIVCLSDFMTAQDREAGRLVQILARHTQDVRQPIHAVYYRNTAISSRIASF
VDYLVDALGGGNAAQKAAAWTRP
>Mature_343_residues
MMGIHESGNDAAQCARFTFAQKVAYKRNYFDFKSTMKVTLDELQAFAAVVDTGSITAAAQQLGLTVSATSRTLARLEGKL
KTTLLRRTTRRLELTEEGRTFLNSARAIIDSVESAEEQMLARREKPSGRLRVDAASPFMLHVIVPLVRGYRERFPRVELE
LNSNEGVIDLLERRTDVAIRIGRLKDSTLHSRLIGNSRLRILASPAYLDAHGQPRKAGDLGKHALLGFNQPESLNVWPIL
GADGEPCRIEPAVWSSSGETLRQLALDGAGIVCLSDFMTAQDREAGRLVQILARHTQDVRQPIHAVYYRNTAISSRIASF
VDYLVDALGGGNAAQKAAAWTRP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=46.0207612456747, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=31.5254237288136, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=27.972027972028, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=26.8041237113402, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=27.5510204081633, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787589, Length=271, Percent_Identity=25.830258302583, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=29.9212598425197, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788481, Length=291, Percent_Identity=25.7731958762887, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082132, Length=295, Percent_Identity=25.7627118644068, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788297, Length=141, Percent_Identity=31.2056737588652, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37854; Mature: 37854

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGIHESGNDAAQCARFTFAQKVAYKRNYFDFKSTMKVTLDELQAFAAVVDTGSITAAAQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QLGLTVSATSRTLARLEGKLKTTLLRRTTRRLELTEEGRTFLNSARAIIDSVESAEEQML
HHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARREKPSGRLRVDAASPFMLHVIVPLVRGYRERFPRVELELNSNEGVIDLLERRTDVAIR
HHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCCEEE
IGRLKDSTLHSRLIGNSRLRILASPAYLDAHGQPRKAGDLGKHALLGFNQPESLNVWPIL
EECCCCHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEE
GADGEPCRIEPAVWSSSGETLRQLALDGAGIVCLSDFMTAQDREAGRLVQILARHTQDVR
CCCCCCCEECCHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QPIHAVYYRNTAISSRIASFVDYLVDALGGGNAAQKAAAWTRP
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MMGIHESGNDAAQCARFTFAQKVAYKRNYFDFKSTMKVTLDELQAFAAVVDTGSITAAAQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QLGLTVSATSRTLARLEGKLKTTLLRRTTRRLELTEEGRTFLNSARAIIDSVESAEEQML
HHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARREKPSGRLRVDAASPFMLHVIVPLVRGYRERFPRVELELNSNEGVIDLLERRTDVAIR
HHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCCEEE
IGRLKDSTLHSRLIGNSRLRILASPAYLDAHGQPRKAGDLGKHALLGFNQPESLNVWPIL
EECCCCHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEE
GADGEPCRIEPAVWSSSGETLRQLALDGAGIVCLSDFMTAQDREAGRLVQILARHTQDVR
CCCCCCCEECCHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QPIHAVYYRNTAISSRIASFVDYLVDALGGGNAAQKAAAWTRP
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]