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The map label for this gene is fdoI [H]

Identifier: 126444462

GI number: 126444462

Start: 2316953

End: 2317579

Strand: Reverse

Name: fdoI [H]

Synonym: BURPS668_A2394

Alternate gene names: 126444462

Gene position: 2317579-2316953 (Counterclockwise)

Preceding gene: 126444663

Following gene: 126445389

Centisome position: 74.1

GC content: 64.27

Gene sequence:

>627_bases
ATGAAACATGACGACCCGAACCTGATCGTCCGCTACACGGCGAACGAGCGCTCGAACCACTGGATCACCGCGATCTCGTT
CGTGCTGCTCGCGCTGTCCGGGCTCGCGCTGTTTCATCCTTCGATGTTCTGGATGACGGCGCTGTTCGGCGGCGGGCAGT
GGACGCGGATCCTGCATCCGTTCGTCGGCCTCGTGATGTTCGCGTCGTTCGCGGTGATGGTCGTGCGCTACTGGCGCCAT
AACCTGCTCGACGCGGGCGACCGCCAGTGGCTGCGGCAAATGGACGATGTGCTCGCGAACCGGGAGGACAAGCTGCCGGA
AGTCGGCCGCTATAACGCCGGACAGAAGCTGCTATTCTTCGTGATGGTGGCGTGTCTGCTGTTGCTGCTCGTCTCCGGGA
TCGTGATCTGGCGGCGCTATTTCTCGCTCTACTTCCCGATCGGCGTGATTCGCGCGGCGGCCGTCGTCCATGCGGCGGCG
GCGTTCGCGCTGATCGTCGGCATCGTCGTGCACGTGTACGCGGCGCTGTGGGTGAAGGGCTCGATCGGCGCGATGGTGCG
CGGCACCGTGACCGTCGGCTGGGCCAGAAAGCACCACCCGAAGTGGTTTCGCGAGAGCGTGAAGTAA

Upstream 100 bases:

>100_bases
TCGGCTTCTTCCACTACACGCGCGTCGGCCCGAACGACGTGACGGACGACGAGGAGGCCGCCGCCCGTGACGAAGCGCGC
CGCATCCAGGAGGACGCGAA

Downstream 100 bases:

>100_bases
TATCGCATCGCGCGAAGCCGGCGCGGCGCGCGGGCCCGGGCGGTCCGCGCGCCGCAAGCCGCGCGCCCGTCGGGCCGCCG
GGCCGCCGGGCCGCCCCTGC

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHPFVGLVMFASFAVMVVRYWRH
NLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFFVMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAA
AFALIVGIVVHVYAALWVKGSIGAMVRGTVTVGWARKHHPKWFRESVK

Sequences:

>Translated_208_residues
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHPFVGLVMFASFAVMVVRYWRH
NLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFFVMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAA
AFALIVGIVVHVYAALWVKGSIGAMVRGTVTVGWARKHHPKWFRESVK
>Mature_208_residues
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHPFVGLVMFASFAVMVVRYWRH
NLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFFVMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAA
AFALIVGIVVHVYAALWVKGSIGAMVRGTVTVGWARKHHPKWFRESVK

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=208, Percent_Identity=54.8076923076923, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1787750, Length=205, Percent_Identity=45.3658536585366, Blast_Score=193, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 23635; Mature: 23635

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHP
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FVGLVMFASFAVMVVRYWRHNLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
VMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAAAFALIVGIVVHVYAALWVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIGAMVRGTVTVGWARKHHPKWFRESVK
CCCHHHHHHEEECHHHHCCCHHHHHCCC
>Mature Secondary Structure
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHP
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FVGLVMFASFAVMVVRYWRHNLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
VMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAAAFALIVGIVVHVYAALWVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIGAMVRGTVTVGWARKHHPKWFRESVK
CCCHHHHHHEEECHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]