Click here to switch to the map view.

The map label for this gene is cioA [H]

Identifier: 126444426

GI number: 126444426

Start: 391882

End: 393294

Strand: Reverse

Name: cioA [H]

Synonym: BURPS668_A0428

Alternate gene names: 126444426

Gene position: 393294-391882 (Counterclockwise)

Preceding gene: 126443918

Following gene: 126445403

Centisome position: 12.58

GC content: 66.6

Gene sequence:

>1413_bases
ATGGAAACCGCTTTTTCGGCCCTCGATCTGGCCCGCGTGCAGTTCGCGTTCACCGTCTCCTTCCATATCGTCTTTCCCGC
GCTGAGCATCGGGCTCGCGAGCTTCATCGCCGTGCTCGAATACCGATGGCTCAAGACCGGCAAGGCGTATTACAAGAGCC
TTTGCCTGTTCTGGTCGAAGATCTTCGCGGTCGCGTTCGGCATGGGCGTCGTCTCCGGCGTCGTGATGGCGTATCAGTTC
GGCACGAACTGGTCCGGCTTCTCGAGCTTCGCCGGCTCCGTCACCGGCCCGCTGCTGATGTACGAGGTGATGACCGCGTT
CTTCCTCGAAGCGGGCTTCCTCGGCATCATGCTGTTCGGCTGGAACCGCGTGAGCCCGCGCGCGCACTTCGGCGCGACGC
TGATGGTCGCGATCGGCACGCTGATCTCGACGTTCTGGATTCTCGCGTCGAACAGCTGGATGCAGACGCCGCAGGGCTTC
GAGATCGTCGACGGCCAGGTCGTGCCCACCGACTGGTTCGCGATCATCTTCAACCCGTCGTTCCCGTACCGGCTCGCGCA
CATGGCGATCGCCGCGTTCGTCGTCGCCGCGCTCGTGGTGGCCGCGACGGCCGCGTGGCACCTGCTGAAGGGCCGCCGCG
ACAAGGCCGTGAAGAAGATGTTCTCGATGTCGCTGTGGCTGTTGCTCGTGCTCGCGCCGCTGCAGGCCGTGATCGGCGAT
CAGCACGGCATCAACACGCTGCGCCACCAGCCCGCGAAGCTCGCCGCGATCGAGGGGCTGTGGGAAACCGAGCCGGGCGG
CACCGCGCTGAACCTGTTCGGCATTCCCGACATGAAGGCGCAAACCACGCGTTACGCGGTGTCGATTCCGCATCTCGGCA
GCCTGATCCTCACGCACAGCTGGGACGGAGAGATCCGCGGGCTGAAGTCGTTCCCGCCGCAAGACCGGCCGAACTCGACC
GTCGTGTTCTGGAGCTTTCGCATCATGGTCGGGCTCGGCCTCGCGATGATCGGCCTCGCCGCGCTCGCCTGGCTGCTGCG
CCGCCGCGGGCGCCTCTATGAAGCGAAGTGGTTCCACCGGTTCGCGCTCGCGATGGGCCCGACGGGCTTCGTCTCCCTGC
TCGCCGGCTGGGTGACGACCGAAGTGGGCCGCCAGCCGTGGGTAGTCTACGGCGTGATGCGCACGTCGCACGCGGTGTCG
CCGCTGTCGACGCAGCAGGTGAGCGTCTCGCTGATGACGTTCGTCGTCGTCTACTTCCTCGTGTTCGGCACGGGCGTCTA
CTACATGCTGAAGCTGATGAGAAAGGGCCCCGCGCTGCCGGGCGACACGCCCGACGATGCGCACGACGCGCGGCCCGAGC
GCACCGCCCGGCGCCCGCTGTCGGCCGCGAACCAGTTGATCGACGCCGCGTGA

Upstream 100 bases:

>100_bases
GCGATACTCGGCTCCGACTCTACGGCCGCGGCCTTGCAGGCGCGGCGCAGGGGCCGCCGGCAGCCCCGCCGGCGGGTGTC
GTCAGCTTCGGTCTACTCGC

Downstream 100 bases:

>100_bases
AGCCGGCGCGCCACCAAGCCAAGCGAGGTTAGCAAATGGATGTCACCGTAATCTGGGCCGCGATCATCGCACTCGGCCTG
TTCATGTACGTCGTGCTCGA

Product: cyanide-insensitive terminal oxidase CioA

Products: Ubiquinone-8; H2O. [C]

Alternate protein names: Cytochrome d ubiquinol oxidase subunit I [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
METAFSALDLARVQFAFTVSFHIVFPALSIGLASFIAVLEYRWLKTGKAYYKSLCLFWSKIFAVAFGMGVVSGVVMAYQF
GTNWSGFSSFAGSVTGPLLMYEVMTAFFLEAGFLGIMLFGWNRVSPRAHFGATLMVAIGTLISTFWILASNSWMQTPQGF
EIVDGQVVPTDWFAIIFNPSFPYRLAHMAIAAFVVAALVVAATAAWHLLKGRRDKAVKKMFSMSLWLLLVLAPLQAVIGD
QHGINTLRHQPAKLAAIEGLWETEPGGTALNLFGIPDMKAQTTRYAVSIPHLGSLILTHSWDGEIRGLKSFPPQDRPNST
VVFWSFRIMVGLGLAMIGLAALAWLLRRRGRLYEAKWFHRFALAMGPTGFVSLLAGWVTTEVGRQPWVVYGVMRTSHAVS
PLSTQQVSVSLMTFVVVYFLVFGTGVYYMLKLMRKGPALPGDTPDDAHDARPERTARRPLSAANQLIDAA

Sequences:

>Translated_470_residues
METAFSALDLARVQFAFTVSFHIVFPALSIGLASFIAVLEYRWLKTGKAYYKSLCLFWSKIFAVAFGMGVVSGVVMAYQF
GTNWSGFSSFAGSVTGPLLMYEVMTAFFLEAGFLGIMLFGWNRVSPRAHFGATLMVAIGTLISTFWILASNSWMQTPQGF
EIVDGQVVPTDWFAIIFNPSFPYRLAHMAIAAFVVAALVVAATAAWHLLKGRRDKAVKKMFSMSLWLLLVLAPLQAVIGD
QHGINTLRHQPAKLAAIEGLWETEPGGTALNLFGIPDMKAQTTRYAVSIPHLGSLILTHSWDGEIRGLKSFPPQDRPNST
VVFWSFRIMVGLGLAMIGLAALAWLLRRRGRLYEAKWFHRFALAMGPTGFVSLLAGWVTTEVGRQPWVVYGVMRTSHAVS
PLSTQQVSVSLMTFVVVYFLVFGTGVYYMLKLMRKGPALPGDTPDDAHDARPERTARRPLSAANQLIDAA
>Mature_470_residues
METAFSALDLARVQFAFTVSFHIVFPALSIGLASFIAVLEYRWLKTGKAYYKSLCLFWSKIFAVAFGMGVVSGVVMAYQF
GTNWSGFSSFAGSVTGPLLMYEVMTAFFLEAGFLGIMLFGWNRVSPRAHFGATLMVAIGTLISTFWILASNSWMQTPQGF
EIVDGQVVPTDWFAIIFNPSFPYRLAHMAIAAFVVAALVVAATAAWHLLKGRRDKAVKKMFSMSLWLLLVLAPLQAVIGD
QHGINTLRHQPAKLAAIEGLWETEPGGTALNLFGIPDMKAQTTRYAVSIPHLGSLILTHSWDGEIRGLKSFPPQDRPNST
VVFWSFRIMVGLGLAMIGLAALAWLLRRRGRLYEAKWFHRFALAMGPTGFVSLLAGWVTTEVGRQPWVVYGVMRTSHAVS
PLSTQQVSVSLMTFVVVYFLVFGTGVYYMLKLMRKGPALPGDTPDDAHDARPERTARRPLSAANQLIDAA

Specific function: Cytochrome D Terminal Oxidase Complex Is The Component Of The Aerobic Respiratory Chain Of E.Coli That Predominates When Cells Are Grown At Low Aeration. [C]

COG id: COG1271

COG function: function code C; Cytochrome bd-type quinol oxidase, subunit 1

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome ubiquinol oxidase subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI87081779, Length=502, Percent_Identity=31.2749003984064, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1787212, Length=317, Percent_Identity=35.6466876971609, Blast_Score=222, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002585 [H]

Pfam domain/function: PF01654 Bac_Ubq_Cox [H]

EC number: 1.10.3.- [C]

Molecular weight: Translated: 51809; Mature: 51809

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METAFSALDLARVQFAFTVSFHIVFPALSIGLASFIAVLEYRWLKTGKAYYKSLCLFWSK
CCCHHHHHHHHHHHEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFAVAFGMGVVSGVVMAYQFGTNWSGFSSFAGSVTGPLLMYEVMTAFFLEAGFLGIMLFG
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEC
WNRVSPRAHFGATLMVAIGTLISTFWILASNSWMQTPQGFEIVDGQVVPTDWFAIIFNPS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEECCCEEEEEECCC
FPYRLAHMAIAAFVVAALVVAATAAWHLLKGRRDKAVKKMFSMSLWLLLVLAPLQAVIGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QHGINTLRHQPAKLAAIEGLWETEPGGTALNLFGIPDMKAQTTRYAVSIPHLGSLILTHS
CCCCHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCEEEEEC
WDGEIRGLKSFPPQDRPNSTVVFWSFRIMVGLGLAMIGLAALAWLLRRRGRLYEAKWFHR
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FALAMGPTGFVSLLAGWVTTEVGRQPWVVYGVMRTSHAVSPLSTQQVSVSLMTFVVVYFL
HHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHH
VFGTGVYYMLKLMRKGPALPGDTPDDAHDARPERTARRPLSAANQLIDAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure
METAFSALDLARVQFAFTVSFHIVFPALSIGLASFIAVLEYRWLKTGKAYYKSLCLFWSK
CCCHHHHHHHHHHHEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFAVAFGMGVVSGVVMAYQFGTNWSGFSSFAGSVTGPLLMYEVMTAFFLEAGFLGIMLFG
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEC
WNRVSPRAHFGATLMVAIGTLISTFWILASNSWMQTPQGFEIVDGQVVPTDWFAIIFNPS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEECCCEEEEEECCC
FPYRLAHMAIAAFVVAALVVAATAAWHLLKGRRDKAVKKMFSMSLWLLLVLAPLQAVIGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QHGINTLRHQPAKLAAIEGLWETEPGGTALNLFGIPDMKAQTTRYAVSIPHLGSLILTHS
CCCCHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCEEEEEC
WDGEIRGLKSFPPQDRPNSTVVFWSFRIMVGLGLAMIGLAALAWLLRRRGRLYEAKWFHR
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FALAMGPTGFVSLLAGWVTTEVGRQPWVVYGVMRTSHAVSPLSTQQVSVSLMTFVVVYFL
HHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHH
VFGTGVYYMLKLMRKGPALPGDTPDDAHDARPERTARRPLSAANQLIDAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ubiquinol-8; O(2) [C]

Specific reaction: Ubiquinol-8 + O(2) = Ubiquinone-8 + H2O. [C]

General reaction: Oxidoreductases; Acting on diphenols and related substances as donors; With oxygen as acceptor [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]