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The map label for this gene is metZ [H]

Identifier: 126444271

GI number: 126444271

Start: 2354663

End: 2355934

Strand: Reverse

Name: metZ [H]

Synonym: BURPS668_A2437

Alternate gene names: 126444271

Gene position: 2355934-2354663 (Counterclockwise)

Preceding gene: 126445014

Following gene: 126445502

Centisome position: 75.33

GC content: 66.9

Gene sequence:

>1272_bases
ATGAGCGGGCTTTTTGTTGGTCTGCGCATTCGCCGCGCGACGCGCACGGCGGCGATGCGCTTGAAAACGGATGAACGGAA
CATGGACGACTCTCTCAACTTCGACACGCTCGCCGTCCGCTCGGGCACGCTGCGCAGTGAATTCAACGAGCATTCGGAAG
CGCTCTTCCTCACGTCGAGCTTCTGCTACGCGAGCGCGGCCGAGGCGGCCGAGCGCTTCAAGCATTCGGAAGACTACTAC
ACGTACTCGCGCTTCACGAATCCGACCGTCACGATGTTCCAGGATCGCCTCGCGGCGCTCGAGGGCGGCGAGGCGTGCAT
CGCGACCGCGTCCGGAATGGCGGCGATCACGTCGGTCGTGATGGCTGCGCTGCAGGCGGGCGATCACCTCGTCAGCTCGC
GCAGCCTGTTCGGCTCGACGCTCGGGCTGTTCGGGCAGATCTTCGCGAAGTTCGGCATCGAGACGACGTTCGTCGATCCG
GCCGATCTCGACGCATGGCGCGCCGCCGTGCGCCCCGAGACGAAGATGTTCTTCCTCGAGACGCCGTCGAACCCGATGAC
CGAGCTCGCCGACATCGAGGCGGTGGGCAGGATCGCGAAGGCGGCCCATGCGCTGTTCGTCGTCGACAACTGTTTCTGCA
GCCCGGTGCTGCAGCAGCCGCTCAAGCTCGGCGCGGACGTCGTGATGCATTCGGCCACCAAGTTCCTCGACGGTCAGGGG
CGCGTGCTCGGCGGCGCGCTCGTCGGCTCGAAGGCGTTCATCATGGAGAAGGTGTTCCCGTTCGTGCGCACCGCGGGGCC
GACGCTGTCCGCGTTCAATGCGTGGGTGCTGCTCAAGGGCATGGAGACGCTGTCGCTGCGCGTGAACCGGCAATCGGAGA
ACGCGCTCGAGATCGCCCGCTGGCTCGAGGCGCATCCGGCCGTGAAGCGCGTGTTCTATCCGGGGCTCGAATCGCATCCG
CAGCATGCGCTCGCGAAGCGCCAGCAGAAATCGGGCGGCTCGGTCGTGTCGTTCGAGCTGAACGGCGATACGCCGGAGCA
GCAGCGCGCGAACGCGTGGCGTGTGATCGACGGCACGAAGATCGTATCGATCACCGCGAACCTCGGCGACACGCGCACGA
CGATCACGCATCCGGCGACGACGACGCACAGCCGGATCGCGCCTGAAGCGCGCGAGGCGGCGGGGATCACCGAAGGGCTG
ATCCGGCTCGCGGTCGGGCTCGAGGATCCGGCCGACATCCGCGACGATCTCGCGCGCGGGCTCGCCGGCTGA

Upstream 100 bases:

>100_bases
GGCAACCCGAAACAGCTAAAGCGAAGGCCGGCGAGCAGCGCCATGCCGGCCCGAGTCGATAGCTGTTCGTACCGCACACC
AAGCCCGCTGATGCCGACGC

Downstream 100 bases:

>100_bases
CGGCTGACGGCTGACGGCGGGCGGCGGGCGGCTCGGCGGCGTGTGTGCGCGGCGCGTGCAGGGGCTGCGGGCGCGCGAAG
CGGTTCGCTGCGTTCATCCC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSGLFVGLRIRRATRTAAMRLKTDERNMDDSLNFDTLAVRSGTLRSEFNEHSEALFLTSSFCYASAAEAAERFKHSEDYY
TYSRFTNPTVTMFQDRLAALEGGEACIATASGMAAITSVVMAALQAGDHLVSSRSLFGSTLGLFGQIFAKFGIETTFVDP
ADLDAWRAAVRPETKMFFLETPSNPMTELADIEAVGRIAKAAHALFVVDNCFCSPVLQQPLKLGADVVMHSATKFLDGQG
RVLGGALVGSKAFIMEKVFPFVRTAGPTLSAFNAWVLLKGMETLSLRVNRQSENALEIARWLEAHPAVKRVFYPGLESHP
QHALAKRQQKSGGSVVSFELNGDTPEQQRANAWRVIDGTKIVSITANLGDTRTTITHPATTTHSRIAPEAREAAGITEGL
IRLAVGLEDPADIRDDLARGLAG

Sequences:

>Translated_423_residues
MSGLFVGLRIRRATRTAAMRLKTDERNMDDSLNFDTLAVRSGTLRSEFNEHSEALFLTSSFCYASAAEAAERFKHSEDYY
TYSRFTNPTVTMFQDRLAALEGGEACIATASGMAAITSVVMAALQAGDHLVSSRSLFGSTLGLFGQIFAKFGIETTFVDP
ADLDAWRAAVRPETKMFFLETPSNPMTELADIEAVGRIAKAAHALFVVDNCFCSPVLQQPLKLGADVVMHSATKFLDGQG
RVLGGALVGSKAFIMEKVFPFVRTAGPTLSAFNAWVLLKGMETLSLRVNRQSENALEIARWLEAHPAVKRVFYPGLESHP
QHALAKRQQKSGGSVVSFELNGDTPEQQRANAWRVIDGTKIVSITANLGDTRTTITHPATTTHSRIAPEAREAAGITEGL
IRLAVGLEDPADIRDDLARGLAG
>Mature_422_residues
SGLFVGLRIRRATRTAAMRLKTDERNMDDSLNFDTLAVRSGTLRSEFNEHSEALFLTSSFCYASAAEAAERFKHSEDYYT
YSRFTNPTVTMFQDRLAALEGGEACIATASGMAAITSVVMAALQAGDHLVSSRSLFGSTLGLFGQIFAKFGIETTFVDPA
DLDAWRAAVRPETKMFFLETPSNPMTELADIEAVGRIAKAAHALFVVDNCFCSPVLQQPLKLGADVVMHSATKFLDGQGR
VLGGALVGSKAFIMEKVFPFVRTAGPTLSAFNAWVLLKGMETLSLRVNRQSENALEIARWLEAHPAVKRVFYPGLESHPQ
HALAKRQQKSGGSVVSFELNGDTPEQQRANAWRVIDGTKIVSITANLGDTRTTITHPATTTHSRIAPEAREAAGITEGLI
RLAVGLEDPADIRDDLARGLAG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=329, Percent_Identity=36.1702127659575, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI299473759, Length=329, Percent_Identity=32.5227963525836, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI25453487, Length=328, Percent_Identity=30.7926829268293, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1790375, Length=387, Percent_Identity=36.6925064599483, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789383, Length=346, Percent_Identity=28.3236994219653, Blast_Score=144, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=37.9518072289157, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=332, Percent_Identity=37.0481927710843, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI71980708, Length=346, Percent_Identity=35.5491329479769, Blast_Score=219, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=33.256351039261, Blast_Score=212, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6319307, Length=401, Percent_Identity=31.6708229426434, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=27.5862068965517, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6321170, Length=293, Percent_Identity=29.0102389078498, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=349, Percent_Identity=26.3610315186246, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922554, Length=346, Percent_Identity=35.8381502890173, Blast_Score=236, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 45904; Mature: 45773

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLFVGLRIRRATRTAAMRLKTDERNMDDSLNFDTLAVRSGTLRSEFNEHSEALFLTSS
CCCEEEEEEEHHHHHHHHHEEECCCCCCCCCCCCCEEEEECCCHHHHHCCCCCEEEEEHH
FCYASAAEAAERFKHSEDYYTYSRFTNPTVTMFQDRLAALEGGEACIATASGMAAITSVV
HHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
MAALQAGDHLVSSRSLFGSTLGLFGQIFAKFGIETTFVDPADLDAWRAAVRPETKMFFLE
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHCCCCCEEEEEE
TPSNPMTELADIEAVGRIAKAAHALFVVDNCFCSPVLQQPLKLGADVVMHSATKFLDGQG
CCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
RVLGGALVGSKAFIMEKVFPFVRTAGPTLSAFNAWVLLKGMETLSLRVNRQSENALEIAR
CEEEHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCEEEEEEECCCCCHHHHHHH
WLEAHPAVKRVFYPGLESHPQHALAKRQQKSGGSVVSFELNGDTPEQQRANAWRVIDGTK
HHHHCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCEEEECCCE
IVSITANLGDTRTTITHPATTTHSRIAPEAREAAGITEGLIRLAVGLEDPADIRDDLARG
EEEEEECCCCCCEEEECCCCCCHHHCCCHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
LAG
CCC
>Mature Secondary Structure 
SGLFVGLRIRRATRTAAMRLKTDERNMDDSLNFDTLAVRSGTLRSEFNEHSEALFLTSS
CCEEEEEEEHHHHHHHHHEEECCCCCCCCCCCCCEEEEECCCHHHHHCCCCCEEEEEHH
FCYASAAEAAERFKHSEDYYTYSRFTNPTVTMFQDRLAALEGGEACIATASGMAAITSVV
HHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
MAALQAGDHLVSSRSLFGSTLGLFGQIFAKFGIETTFVDPADLDAWRAAVRPETKMFFLE
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHCCCCCEEEEEE
TPSNPMTELADIEAVGRIAKAAHALFVVDNCFCSPVLQQPLKLGADVVMHSATKFLDGQG
CCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
RVLGGALVGSKAFIMEKVFPFVRTAGPTLSAFNAWVLLKGMETLSLRVNRQSENALEIAR
CEEEHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCEEEEEEECCCCCHHHHHHH
WLEAHPAVKRVFYPGLESHPQHALAKRQQKSGGSVVSFELNGDTPEQQRANAWRVIDGTK
HHHHCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCEEEECCCE
IVSITANLGDTRTTITHPATTTHSRIAPEAREAAGITEGLIRLAVGLEDPADIRDDLARG
EEEEEECCCCCCEEEECCCCCCHHHCCCHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
LAG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]