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The map label for this gene is cmlA [H]

Identifier: 126444208

GI number: 126444208

Start: 1569847

End: 1571097

Strand: Reverse

Name: cmlA [H]

Synonym: BURPS668_A1641

Alternate gene names: 126444208

Gene position: 1571097-1569847 (Counterclockwise)

Preceding gene: 126444062

Following gene: 126445398

Centisome position: 50.24

GC content: 68.82

Gene sequence:

>1251_bases
ATGAGCGAGACCTCGAAGCGCCATCCTTCCCTCGTGTTGATCCTGATTCCGGTCGTGATGCTCGGCCAGCTCTCGATGGA
CATGTATCTGCCCGCGCTCGACAGCCTGCGGGCCAGCATGCGCGCGACGGGCAGCGAGCTGCAGCTCACGCTCAGCGCGT
TCGTCGTCTCGCTCGGCGTATCGCAGTTCCTCGTCGGCGAGTTCGCGCGCCGCGCGGGGCGAGGGCGCGCGATCCGGATC
GCGCTCGTCGGCTTCGCCGGCGCGACGGCCGGCTGCGCGATCACGTCGTCGATCGCGTTCTTTATCGTCTGCCGCGCGGT
TCAGGGCATCTCGGCGGCGGTGTGCGTCGCGCTGTCGTACGCGGTGATCGCCGATAACGCGAAGGACAGCCATGAAAGCA
TGCGCGGGATGACGATGCTCACGGTCGCCTCGACGGTCGCGCCGCTGATCGCGCCGGTCGCGGGCTCGCAGGTGCTGCAG
CTCGCCGGCTCGTGGCGGGCCGTCTTCGTGCTGCTGCTATGCGTCGCGAGCGCGGCGACGCTCGCGTTCTTCACGCAACC
GGCCGCGCGCTTCGATGCGCGCGCGTCGGACGCCTCGAAGCCGGCGCGCCAGATCTATCTCGGGATCGCGCGGTCTTCCC
GGTTCTGGCGCTACGCGCTGCTTTGCTCGATCGGCACGACGATCAATTTCTGCTTCTTCTCGTTCCTGCCCGGCATCCTG
CTGGTACAGAACGGGCTTACGCAGAACGCGTTTTCCGCCGCGTTCTTCCTGTGCGGGCTGACCGAGATCGCGGGCTCGCT
GCTCGCGCCGAGAATCAGCAAGCGCATCGGGCACCCCGCATTGACGGGGCTCGCGTGCGCGTGCTGCGCGCTCGGCGGTT
TCGGCGTCGCGTATCTGTCGACGATGCAGGGGGCGGTCGTGCCGATCGTCGTCGCGTTCTCGATCGTCGAGCTGGGAACC
GGCATGCTGATCGCGCCGTGCTTCTCGGCGGCGCTGTATCTGTTCCGCTCGGCGTCCAACGAAGCGTCCGCGATCTGCGG
GTTCCAGCAATTCATGATCGCCGCCGGCGTCGCCACGTTCGCCGCTGCGATGCAATGGCAATCGTCGTATGCGCTCGGCA
TCCTGATCGGCGTGGCCGCGCTGCTCGGCGCGGCGCTCGCCTGCACGCCGGCGGGCGGCGCTGCGGCCGACGAGCGAGCC
GATGAGCGAGCGGACGAGCGCGTGGCGCGGGTGGCGGCGAATCCGGATTAG

Upstream 100 bases:

>100_bases
ATGCTGGGCCTCGCGCTGGACGCCGAAAGCACGCTCGCCGGCTGAGCGGCGCCGCCTTCCCTGCCTTTTTTCGAATCGTC
ATTTCCTGTCGACCACGTTC

Downstream 100 bases:

>100_bases
CGGCGAGGCGCCCGCGCGGGCGCGCGGCGGCCGTTGGCCGAGCGACGCGCGGGGAAGGACGAAGGCGGGGCGCGACGCAC
GCCGTGCAAGCGAGGCGAAC

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSETSKRHPSLVLILIPVVMLGQLSMDMYLPALDSLRASMRATGSELQLTLSAFVVSLGVSQFLVGEFARRAGRGRAIRI
ALVGFAGATAGCAITSSIAFFIVCRAVQGISAAVCVALSYAVIADNAKDSHESMRGMTMLTVASTVAPLIAPVAGSQVLQ
LAGSWRAVFVLLLCVASAATLAFFTQPAARFDARASDASKPARQIYLGIARSSRFWRYALLCSIGTTINFCFFSFLPGIL
LVQNGLTQNAFSAAFFLCGLTEIAGSLLAPRISKRIGHPALTGLACACCALGGFGVAYLSTMQGAVVPIVVAFSIVELGT
GMLIAPCFSAALYLFRSASNEASAICGFQQFMIAAGVATFAAAMQWQSSYALGILIGVAALLGAALACTPAGGAAADERA
DERADERVARVAANPD

Sequences:

>Translated_416_residues
MSETSKRHPSLVLILIPVVMLGQLSMDMYLPALDSLRASMRATGSELQLTLSAFVVSLGVSQFLVGEFARRAGRGRAIRI
ALVGFAGATAGCAITSSIAFFIVCRAVQGISAAVCVALSYAVIADNAKDSHESMRGMTMLTVASTVAPLIAPVAGSQVLQ
LAGSWRAVFVLLLCVASAATLAFFTQPAARFDARASDASKPARQIYLGIARSSRFWRYALLCSIGTTINFCFFSFLPGIL
LVQNGLTQNAFSAAFFLCGLTEIAGSLLAPRISKRIGHPALTGLACACCALGGFGVAYLSTMQGAVVPIVVAFSIVELGT
GMLIAPCFSAALYLFRSASNEASAICGFQQFMIAAGVATFAAAMQWQSSYALGILIGVAALLGAALACTPAGGAAADERA
DERADERVARVAANPD
>Mature_415_residues
SETSKRHPSLVLILIPVVMLGQLSMDMYLPALDSLRASMRATGSELQLTLSAFVVSLGVSQFLVGEFARRAGRGRAIRIA
LVGFAGATAGCAITSSIAFFIVCRAVQGISAAVCVALSYAVIADNAKDSHESMRGMTMLTVASTVAPLIAPVAGSQVLQL
AGSWRAVFVLLLCVASAATLAFFTQPAARFDARASDASKPARQIYLGIARSSRFWRYALLCSIGTTINFCFFSFLPGILL
VQNGLTQNAFSAAFFLCGLTEIAGSLLAPRISKRIGHPALTGLACACCALGGFGVAYLSTMQGAVVPIVVAFSIVELGTG
MLIAPCFSAALYLFRSASNEASAICGFQQFMIAAGVATFAAAMQWQSSYALGILIGVAALLGAALACTPAGGAAADERAD
ERADERVARVAANPD

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=284, Percent_Identity=28.5211267605634, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082312, Length=355, Percent_Identity=26.4788732394366, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI48994889, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790146, Length=155, Percent_Identity=25.1612903225806, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43171; Mature: 43040

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00213 LIPOCALIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETSKRHPSLVLILIPVVMLGQLSMDMYLPALDSLRASMRATGSELQLTLSAFVVSLGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SQFLVGEFARRAGRGRAIRIALVGFAGATAGCAITSSIAFFIVCRAVQGISAAVCVALSY
HHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
AVIADNAKDSHESMRGMTMLTVASTVAPLIAPVAGSQVLQLAGSWRAVFVLLLCVASAAT
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHH
LAFFTQPAARFDARASDASKPARQIYLGIARSSRFWRYALLCSIGTTINFCFFSFLPGIL
HHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVQNGLTQNAFSAAFFLCGLTEIAGSLLAPRISKRIGHPALTGLACACCALGGFGVAYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
TMQGAVVPIVVAFSIVELGTGMLIAPCFSAALYLFRSASNEASAICGFQQFMIAAGVATF
HHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AAAMQWQSSYALGILIGVAALLGAALACTPAGGAAADERADERADERVARVAANPD
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SETSKRHPSLVLILIPVVMLGQLSMDMYLPALDSLRASMRATGSELQLTLSAFVVSLGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SQFLVGEFARRAGRGRAIRIALVGFAGATAGCAITSSIAFFIVCRAVQGISAAVCVALSY
HHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
AVIADNAKDSHESMRGMTMLTVASTVAPLIAPVAGSQVLQLAGSWRAVFVLLLCVASAAT
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHH
LAFFTQPAARFDARASDASKPARQIYLGIARSSRFWRYALLCSIGTTINFCFFSFLPGIL
HHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVQNGLTQNAFSAAFFLCGLTEIAGSLLAPRISKRIGHPALTGLACACCALGGFGVAYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
TMQGAVVPIVVAFSIVELGTGMLIAPCFSAALYLFRSASNEASAICGFQQFMIAAGVATF
HHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AAAMQWQSSYALGILIGVAALLGAALACTPAGGAAADERADERADERVARVAANPD
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]