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The map label for this gene is ald [H]

Identifier: 126444204

GI number: 126444204

Start: 1474713

End: 1475828

Strand: Reverse

Name: ald [H]

Synonym: BURPS668_A1536

Alternate gene names: 126444204

Gene position: 1475828-1474713 (Counterclockwise)

Preceding gene: 126443000

Following gene: 126442734

Centisome position: 47.19

GC content: 70.88

Gene sequence:

>1116_bases
ATGCTGATCGGTGTGCCAAAGGAGATCAAGGTCTACGAATATCGCGTCGGCCTGACGCCGGCGGGCGCGCGCGAGCTCGC
GCTGCACGGCCATCAGGTGCTGGTGCAGCGCGGTGCGGGCGCGGCGATCGGCTTGCAGGACGACGATTACGCGGCGGCCG
GCGCGATGCTGGCCGACGACGCGGCCGACGTGTACGCGCGCGCCGACATGATCGTCAAGGTCAAGGAGCCGCAGCCGGCC
GAATGTGCGATGCTGCGGCGCGGCCAGATCCTGTTCGCCTACCTGCATCTCGCGCCCGATCCCGATCAGGCCGCGGCGCT
CGTCAAGTCGCGCGCGGTCTGCATCGCGTACGAAACCGTGCGCGCGCCCGGAGGCGGCTTGCCGCTGCTCGCGCCGATGA
GCGAAGTGGCCGGCCGCATGTCGATTCAGGTTGCCGCGGCGCATCTCGAAAGCCCCGGCGGCGGGATGGGCCTGCTGATG
GCGGGCGTGCCCGGCGTGCCGGCCGCGCACGTCGTCGTGATCGGCGCGGGCGTCGTCGGCACCTGCGCGCTGCAAATGGC
GGCGGGCCTCGGCGCGCGCGTGACCGTGCTCGACACCAACGTCGATCGGCTGCGCGAGCTCGACCTCGTCTTCGGCAACC
GAATCGCGACCCTCTACTCGAACACGCATGCGATCGACGGTGCGGTGCGCGGCGCGGACGTCGTGATCGGCGCGGTGCTG
GTGCCCGGCGCGTCCGCGCCGCGGCTCGTCACGCGCGAGATGATCTCGAAGATGCGCGCCGGCGCGGTCGTCGTCGATGT
CGCGATCGACCAGGGCGGCTGCATCGAGACATCCCACCCGACGACGCACGCGCAGCCGACCTACGTCGTCGACGGCGTCG
TGCATTACTGCGTGGCCAACATGCCCGGCGCGGTCGCGCGCACGTCGACGTTCGCGCTCGGCAACGCGACGCTTCGCCAT
GCGATCGCGCTCGCGAACAAAGGCTGGAAGCGCGCGCTCGCCGATGACCCGTACCTGCGCGCGGGGCTGAATGTGTGCGA
CGGCCACGTCACGCACGAAGCGGTCGCGCGTGCGCTCGGCCTCGCTTACGTGCATGCGGACGAGATGCTCGATTGA

Upstream 100 bases:

>100_bases
GAGCCGGAAATACGCTCGCCGGCGGCCGGCGCGTGACGGTTGCCGCGGGTTACCGCGCGCGGTCGACTCGGCACCGGACG
CGAACCGGAGGAGACAGATC

Downstream 100 bases:

>100_bases
ATCGTTCAGACGGTCGGGTTCTCGCATGTTCATTGTCGCCGGAGCTATTCGGTGCATCGCCGGCGACATGGTCTGACGGG
GAGCCGGCCAACTGACGATG

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLIGVPKEIKVYEYRVGLTPAGARELALHGHQVLVQRGAGAAIGLQDDDYAAAGAMLADDAADVYARADMIVKVKEPQPA
ECAMLRRGQILFAYLHLAPDPDQAAALVKSRAVCIAYETVRAPGGGLPLLAPMSEVAGRMSIQVAAAHLESPGGGMGLLM
AGVPGVPAAHVVVIGAGVVGTCALQMAAGLGARVTVLDTNVDRLRELDLVFGNRIATLYSNTHAIDGAVRGADVVIGAVL
VPGASAPRLVTREMISKMRAGAVVVDVAIDQGGCIETSHPTTHAQPTYVVDGVVHYCVANMPGAVARTSTFALGNATLRH
AIALANKGWKRALADDPYLRAGLNVCDGHVTHEAVARALGLAYVHADEMLD

Sequences:

>Translated_371_residues
MLIGVPKEIKVYEYRVGLTPAGARELALHGHQVLVQRGAGAAIGLQDDDYAAAGAMLADDAADVYARADMIVKVKEPQPA
ECAMLRRGQILFAYLHLAPDPDQAAALVKSRAVCIAYETVRAPGGGLPLLAPMSEVAGRMSIQVAAAHLESPGGGMGLLM
AGVPGVPAAHVVVIGAGVVGTCALQMAAGLGARVTVLDTNVDRLRELDLVFGNRIATLYSNTHAIDGAVRGADVVIGAVL
VPGASAPRLVTREMISKMRAGAVVVDVAIDQGGCIETSHPTTHAQPTYVVDGVVHYCVANMPGAVARTSTFALGNATLRH
AIALANKGWKRALADDPYLRAGLNVCDGHVTHEAVARALGLAYVHADEMLD
>Mature_371_residues
MLIGVPKEIKVYEYRVGLTPAGARELALHGHQVLVQRGAGAAIGLQDDDYAAAGAMLADDAADVYARADMIVKVKEPQPA
ECAMLRRGQILFAYLHLAPDPDQAAALVKSRAVCIAYETVRAPGGGLPLLAPMSEVAGRMSIQVAAAHLESPGGGMGLLM
AGVPGVPAAHVVVIGAGVVGTCALQMAAGLGARVTVLDTNVDRLRELDLVFGNRIATLYSNTHAIDGAVRGADVVIGAVL
VPGASAPRLVTREMISKMRAGAVVVDVAIDQGGCIETSHPTTHAQPTYVVDGVVHYCVANMPGAVARTSTFALGNATLRH
AIALANKGWKRALADDPYLRAGLNVCDGHVTHEAVARALGLAYVHADEMLD

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=366, Percent_Identity=29.2349726775956, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI122939153, Length=366, Percent_Identity=29.2349726775956, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1787887, Length=302, Percent_Identity=31.1258278145695, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17550456, Length=380, Percent_Identity=28.6842105263158, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 38428; Mature: 38428

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGVPKEIKVYEYRVGLTPAGARELALHGHQVLVQRGAGAAIGLQDDDYAAAGAMLADD
CCCCCCCCEEEEEEEECCCCCCCHHHHHCCCEEEEECCCCCEECCCCCCCHHHCCCHHCC
AADVYARADMIVKVKEPQPAECAMLRRGQILFAYLHLAPDPDQAAALVKSRAVCIAYETV
HHHHHEECEEEEEECCCCCHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCEEEEEEEEC
RAPGGGLPLLAPMSEVAGRMSIQVAAAHLESPGGGMGLLMAGVPGVPAAHVVVIGAGVVG
CCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEEEEEECCHHH
TCALQMAAGLGARVTVLDTNVDRLRELDLVFGNRIATLYSNTHAIDGAVRGADVVIGAVL
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCEEEEEECCCEECCCCCCCCCEEEEEEE
VPGASAPRLVTREMISKMRAGAVVVDVAIDQGGCIETSHPTTHAQPTYVVDGVVHYCVAN
ECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHC
MPGAVARTSTFALGNATLRHAIALANKGWKRALADDPYLRAGLNVCDGHVTHEAVARALG
CCCCHHHCCEEEECCHHHHHHHHHHCCCHHHHCCCCCHHHHCCHHHCCCHHHHHHHHHHH
LAYVHADEMLD
HHEEEHHHHCC
>Mature Secondary Structure
MLIGVPKEIKVYEYRVGLTPAGARELALHGHQVLVQRGAGAAIGLQDDDYAAAGAMLADD
CCCCCCCCEEEEEEEECCCCCCCHHHHHCCCEEEEECCCCCEECCCCCCCHHHCCCHHCC
AADVYARADMIVKVKEPQPAECAMLRRGQILFAYLHLAPDPDQAAALVKSRAVCIAYETV
HHHHHEECEEEEEECCCCCHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCEEEEEEEEC
RAPGGGLPLLAPMSEVAGRMSIQVAAAHLESPGGGMGLLMAGVPGVPAAHVVVIGAGVVG
CCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEEEEEECCHHH
TCALQMAAGLGARVTVLDTNVDRLRELDLVFGNRIATLYSNTHAIDGAVRGADVVIGAVL
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCEEEEEECCCEECCCCCCCCCEEEEEEE
VPGASAPRLVTREMISKMRAGAVVVDVAIDQGGCIETSHPTTHAQPTYVVDGVVHYCVAN
ECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHC
MPGAVARTSTFALGNATLRHAIALANKGWKRALADDPYLRAGLNVCDGHVTHEAVARALG
CCCCHHHCCEEEECCHHHHHHHHHHCCCHHHHCCCCCHHHHCCHHHCCCHHHHHHHHHHH
LAYVHADEMLD
HHEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]