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The map label for this gene is emrB [C]

Identifier: 126443986

GI number: 126443986

Start: 1275506

End: 1277020

Strand: Reverse

Name: emrB [C]

Synonym: BURPS668_A1356

Alternate gene names: 126443986

Gene position: 1277020-1275506 (Counterclockwise)

Preceding gene: 126445387

Following gene: 126445371

Centisome position: 40.83

GC content: 70.17

Gene sequence:

>1515_bases
ATGTCCCCCGATTCCTCCGCGCCGCCCGCCCATGCCGATGCCGACCGTCCGGCCGCGTCCGTGCGGCTCACGATCGCCGC
GCTGCTGCTCGTGCTGCTGTTGTCCGCGCTCGACCAGACGATCGTGTCGACCGCGCTGCCGACGATCGTCGGCGACCTGG
GCGGCCTCGAGAAGCTGTCGTGGGTCGTCACCGCGTACCTGCTGTCGTCGACCGTCGTGCTGCCGCTGTACGGCAAGCTC
GGCGATCTGTACGGGCGCAAGATCGTGCTGCAGGCGGCGATCGTGCTGTTCGTCGTCGGCTCGGCGCTTTGCGGGCTCGC
GCAGGACATGACGCAGCTGATCGTGCTGCGCGCGCTGCAGGGGCTCGGCGGCGGCGGGCTGATGGTCGTCACGATGGCGG
CGATCGCCGATCTGATTCCGCCCGACGAGCGCGGCCGCTACCAGGGGATGTTCGGCGGCGTGTACGGGATCGCGACCGTG
ATCGGGCCGCTGCTCGGCGGGTTTCTCGTCCAGCATCTGTCGTGGCGCTGGATCTTCTACATCAACCTGCCGCTCGGCGC
GCTCGCGTTCGCGGTGATCGGCGCGGCGTTCAGGCCGCACACCGCATACGTGCGGCGCCGGATCGACTACGCGGGCGCCG
CGTTCCTCGCCACCGCGCTCACCTGCGTCGTGCTGTTCACGAGCCAGGGCGGCACGATCCTGCCGTGGTCGTCGCCGCAA
CTGTGGTTCACGCTCGCGCTCGGCCTCGTCGCGACCGCCGGCTTCGTCTACGAGGAACGCCTCGCCGCGGAGCCGATCCT
GCCGCTCGAGCTGTTTCGCCACCGCACCTTCGTGCTGTGCTGCCTGATCGGCTTCGTCGTCGGGCTCGCGCTGTTCGGCT
CGGTCACGTTCCTGCCGCTCTATCTGCAGGTCGTCAAGGGCTCGACGCCGTCGGAAGCCGGCATGCAGGTGCTGCCGATG
ATGGGCGGGCTGCTCGCGACGTCGATCGGCAGCGGCCGCCTCATCACGACGCTCGGCAAGTATCGTCTGTTCCCGATCGC
GGGCACCTTCGTCGCCTGCGCGGCGATGCTGCTGCTCGCGACGCTGTCGCTCGACACGCCGCTGCGCGCGATGTACCTGT
ACATGGCGCTCCTCGGCGGCGGGCTCGGCATGGTGATGCCCGTCATCGTGCTCGCCGTGCAGAACACCGTCGAATTCCGG
CATCTGGGCGTCGCCACGTCCGGCGCAACGCTGTTTCGCTCGATCGGCGGTTCGCTCGGCGTCGCCGCGTTCGGCGCGCT
GTTCTCGAACGGCCTGCACGCGCGGCTCGCCGCGACGCTGCCGCCCGACACGGAGCTGCCGCCGTCGCTCGGGCCGTCGG
CCGTGCGCCAATTGCCGGGCGCCGTGCGCAGCGCGTATCTGCACGCGTTCGCGAGCTCGCTGCACACGGTGTATCTGTGC
GCGGCCGCGGTGATCGCGATCGCATTCGCGCTCGCGTGGCTCGTCGAAGACGCGCCGCTCAGAAAACGCGCGTAA

Upstream 100 bases:

>100_bases
GGCCGGTAACCAATGGAAATTCTTGTGACGAAGCAGAGATTGACTAGTCAATCGATTGTTTCTACATTCCGTTTGCCCCC
GAACGACAATACGATTGCCC

Downstream 100 bases:

>100_bases
TCGGCGGCGCCGCGCGCGCGGCGCCGCCGCTCGCTCACTGCCCGCTGCGCTTGCACCGCTGCGGGAAAAACTTCAGCTCG
GCGCCGACGCGCAGCGCGTA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSPDSSAPPAHADADRPAASVRLTIAALLLVLLLSALDQTIVSTALPTIVGDLGGLEKLSWVVTAYLLSSTVVLPLYGKL
GDLYGRKIVLQAAIVLFVVGSALCGLAQDMTQLIVLRALQGLGGGGLMVVTMAAIADLIPPDERGRYQGMFGGVYGIATV
IGPLLGGFLVQHLSWRWIFYINLPLGALAFAVIGAAFRPHTAYVRRRIDYAGAAFLATALTCVVLFTSQGGTILPWSSPQ
LWFTLALGLVATAGFVYEERLAAEPILPLELFRHRTFVLCCLIGFVVGLALFGSVTFLPLYLQVVKGSTPSEAGMQVLPM
MGGLLATSIGSGRLITTLGKYRLFPIAGTFVACAAMLLLATLSLDTPLRAMYLYMALLGGGLGMVMPVIVLAVQNTVEFR
HLGVATSGATLFRSIGGSLGVAAFGALFSNGLHARLAATLPPDTELPPSLGPSAVRQLPGAVRSAYLHAFASSLHTVYLC
AAAVIAIAFALAWLVEDAPLRKRA

Sequences:

>Translated_504_residues
MSPDSSAPPAHADADRPAASVRLTIAALLLVLLLSALDQTIVSTALPTIVGDLGGLEKLSWVVTAYLLSSTVVLPLYGKL
GDLYGRKIVLQAAIVLFVVGSALCGLAQDMTQLIVLRALQGLGGGGLMVVTMAAIADLIPPDERGRYQGMFGGVYGIATV
IGPLLGGFLVQHLSWRWIFYINLPLGALAFAVIGAAFRPHTAYVRRRIDYAGAAFLATALTCVVLFTSQGGTILPWSSPQ
LWFTLALGLVATAGFVYEERLAAEPILPLELFRHRTFVLCCLIGFVVGLALFGSVTFLPLYLQVVKGSTPSEAGMQVLPM
MGGLLATSIGSGRLITTLGKYRLFPIAGTFVACAAMLLLATLSLDTPLRAMYLYMALLGGGLGMVMPVIVLAVQNTVEFR
HLGVATSGATLFRSIGGSLGVAAFGALFSNGLHARLAATLPPDTELPPSLGPSAVRQLPGAVRSAYLHAFASSLHTVYLC
AAAVIAIAFALAWLVEDAPLRKRA
>Mature_503_residues
SPDSSAPPAHADADRPAASVRLTIAALLLVLLLSALDQTIVSTALPTIVGDLGGLEKLSWVVTAYLLSSTVVLPLYGKLG
DLYGRKIVLQAAIVLFVVGSALCGLAQDMTQLIVLRALQGLGGGGLMVVTMAAIADLIPPDERGRYQGMFGGVYGIATVI
GPLLGGFLVQHLSWRWIFYINLPLGALAFAVIGAAFRPHTAYVRRRIDYAGAAFLATALTCVVLFTSQGGTILPWSSPQL
WFTLALGLVATAGFVYEERLAAEPILPLELFRHRTFVLCCLIGFVVGLALFGSVTFLPLYLQVVKGSTPSEAGMQVLPMM
GGLLATSIGSGRLITTLGKYRLFPIAGTFVACAAMLLLATLSLDTPLRAMYLYMALLGGGLGMVMPVIVLAVQNTVEFRH
LGVATSGATLFRSIGGSLGVAAFGALFSNGLHARLAATLPPDTELPPSLGPSAVRQLPGAVRSAYLHAFASSLHTVYLCA
AAVIAIAFALAWLVEDAPLRKRA

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=440, Percent_Identity=26.3636363636364, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790195, Length=384, Percent_Identity=29.6875, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081983, Length=288, Percent_Identity=29.5138888888889, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788392, Length=343, Percent_Identity=32.3615160349854, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788710, Length=296, Percent_Identity=25.6756756756757, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790146, Length=161, Percent_Identity=32.2981366459627, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788509, Length=139, Percent_Identity=31.6546762589928, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=298, Percent_Identity=30.5369127516779, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322958, Length=486, Percent_Identity=25.1028806584362, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6319770, Length=476, Percent_Identity=27.3109243697479, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325455, Length=255, Percent_Identity=30.5882352941176, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321663, Length=172, Percent_Identity=31.3953488372093, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324954, Length=386, Percent_Identity=24.6113989637306, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319783, Length=385, Percent_Identity=23.1168831168831, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52876; Mature: 52744

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPDSSAPPAHADADRPAASVRLTIAALLLVLLLSALDQTIVSTALPTIVGDLGGLEKLS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
WVVTAYLLSSTVVLPLYGKLGDLYGRKIVLQAAIVLFVVGSALCGLAQDMTQLIVLRALQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGGGGLMVVTMAAIADLIPPDERGRYQGMFGGVYGIATVIGPLLGGFLVQHLSWRWIFY
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
INLPLGALAFAVIGAAFRPHTAYVRRRIDYAGAAFLATALTCVVLFTSQGGTILPWSSPQ
EECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCH
LWFTLALGLVATAGFVYEERLAAEPILPLELFRHRTFVLCCLIGFVVGLALFGSVTFLPL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQVVKGSTPSEAGMQVLPMMGGLLATSIGSGRLITTLGKYRLFPIAGTFVACAAMLLLA
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHCCEEEEEHHHHHHHHHHHHHHH
TLSLDTPLRAMYLYMALLGGGLGMVMPVIVLAVQNTVEFRHLGVATSGATLFRSIGGSLG
HHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHH
VAAFGALFSNGLHARLAATLPPDTELPPSLGPSAVRQLPGAVRSAYLHAFASSLHTVYLC
HHHHHHHHHCCCHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAVIAIAFALAWLVEDAPLRKRA
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SPDSSAPPAHADADRPAASVRLTIAALLLVLLLSALDQTIVSTALPTIVGDLGGLEKLS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH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HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]