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The map label for this gene is shiA [H]

Identifier: 126443468

GI number: 126443468

Start: 1717054

End: 1718427

Strand: Reverse

Name: shiA [H]

Synonym: BURPS668_A1770

Alternate gene names: 126443468

Gene position: 1718427-1717054 (Counterclockwise)

Preceding gene: 126444650

Following gene: 126442666

Centisome position: 54.95

GC content: 69.36

Gene sequence:

>1374_bases
TTGCCGAATCCGGACGATTCGGCCGGGGCGGACATTCGAGTGAGGAGAAACGCATTGACCCGGAGCATCCCACCGAGCCC
GCCGGCGCGCCAGCCGCGCCGCGCGGCCGGCGCCGCGTTCGTCGGCACGATGATCGAGTGGTACGACTTCTACATCTACG
CGAACGCGGCCGCGCTCGTCTTCGGCGAGCTGTTCTTCCCGTCGCACGATCCGTTCACGAGCACGATGGCGTCGTTCGCG
ACGTTCGCGGTCGGCTTCTTCGCGCGCCCGCTCGGCGGGCTCTTCTTCGGCCATCTCGGCGACCGGATCGGCCGCAAGAA
GGCGCTGATGGCGACGCTCGCGCTGATGGGCGCGGCGACGGTCTGCGTCGGTCTGCTGCCGACCTACGAACGCGTCGGCC
TGCTCGCGCCCGTGCTGCTCGTGCTGCTGCGCATCGTGCAGGGCATCGCGGTGGGCGGCGAGTGGGGCGGCGCGGTGCTG
ATGGCGGGCGAGCATGCGCCGTATGGGCGGCGCACGTTCTTCGCTTCGTTCGCGCAGTTGGGCAGCCCGGCCGGGCTGAT
CCTGTCGCTCGTCGCGTTTCGCGCGGTCGCGTCGATGGAGCACGGCGCGTTCATGTCCTGGGGCTGGCGGCTGCCGTTTC
TCGCGAGCATCGCGCTGCTCGCGGTCGGCGTGTTCGTGCGGCTGAAGGTCGACGAATCGCCCGAGTTCGCGCATGAGAAG
GCGATGTCGCGCACCGTCGCGCGGCCGCTCGCCGAGGTGCTGCGCACGTCGCGCGCGATGCTGCTGTTCTGCCTGTGCGC
GAACACGATCGGCATCGCGGGGCTGTATTTCACGAACACGTTCATGATCGCGTTCACGACGCAGCATGTGGGCGTGACGA
AATCGCTGATCCTCGATTGCCTGTTCATCGTCGCGATCATCCAGTTCGTGACGCAGCCGATCGCCGCGTGGCTCGGCGGC
AAGCTCGGCGACGCACGCTTTCTCAAGCTCGCCGCGCTGTTCGCGATGGCCTCGCCCTATCCGATGTTCGCGCTCGTGCA
GACGGGGCGGCTCGCGCCGATGGTGATCGGCATCGCGATCGCGACCGTGTTTCTCGCCGGTTTCTACTCGGTGATCGCGG
GCTTCGTGAGCGGCGCGTTCCCGACGCGCGTGCGCTATTCGGCGATCTCGATCTCGTACCAGATGTGCGGCGCGATCGCG
GGCGGGCTGACGCCGCTCGTCGGCACGTGGCTCGCGCACCGGTTCGCCGGGCAGTGGTGGCCGCTCGCGGTGTTCTATAC
GTGCCTCGCGGCGCTCTCGCTCGGCGGCGTGATCGCGCTCGACGCGCGCCGCCATCGGCGGGCCGACGTCGCCGACGCCG
TGCTCACGCGCTGA

Upstream 100 bases:

>100_bases
GAAACGATTTTGCATCGTAGAACTGGATTAATGAAATTTATTCTTAAGATAATAACTTAGAATTTGACCGATTAATCCCG
GTGCGCGATAAAACGCCCAT

Downstream 100 bases:

>100_bases
TTCAGGAAAAGGAGGGAGATCTCGGTGGGGGAAATTGACGGTAGGCGGCTCTGGAACAGCCTGACGGAACTCGCGCGAAT
CGGCGGCACCGCGCGCGGCG

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MPNPDDSAGADIRVRRNALTRSIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALVFGELFFPSHDPFTSTMASFA
TFAVGFFARPLGGLFFGHLGDRIGRKKALMATLALMGAATVCVGLLPTYERVGLLAPVLLVLLRIVQGIAVGGEWGGAVL
MAGEHAPYGRRTFFASFAQLGSPAGLILSLVAFRAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEK
AMSRTVARPLAEVLRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDCLFIVAIIQFVTQPIAAWLGG
KLGDARFLKLAALFAMASPYPMFALVQTGRLAPMVIGIAIATVFLAGFYSVIAGFVSGAFPTRVRYSAISISYQMCGAIA
GGLTPLVGTWLAHRFAGQWWPLAVFYTCLAALSLGGVIALDARRHRRADVADAVLTR

Sequences:

>Translated_457_residues
MPNPDDSAGADIRVRRNALTRSIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALVFGELFFPSHDPFTSTMASFA
TFAVGFFARPLGGLFFGHLGDRIGRKKALMATLALMGAATVCVGLLPTYERVGLLAPVLLVLLRIVQGIAVGGEWGGAVL
MAGEHAPYGRRTFFASFAQLGSPAGLILSLVAFRAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEK
AMSRTVARPLAEVLRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDCLFIVAIIQFVTQPIAAWLGG
KLGDARFLKLAALFAMASPYPMFALVQTGRLAPMVIGIAIATVFLAGFYSVIAGFVSGAFPTRVRYSAISISYQMCGAIA
GGLTPLVGTWLAHRFAGQWWPLAVFYTCLAALSLGGVIALDARRHRRADVADAVLTR
>Mature_456_residues
PNPDDSAGADIRVRRNALTRSIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALVFGELFFPSHDPFTSTMASFAT
FAVGFFARPLGGLFFGHLGDRIGRKKALMATLALMGAATVCVGLLPTYERVGLLAPVLLVLLRIVQGIAVGGEWGGAVLM
AGEHAPYGRRTFFASFAQLGSPAGLILSLVAFRAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEKA
MSRTVARPLAEVLRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDCLFIVAIIQFVTQPIAAWLGGK
LGDARFLKLAALFAMASPYPMFALVQTGRLAPMVIGIAIATVFLAGFYSVIAGFVSGAFPTRVRYSAISISYQMCGAIAG
GLTPLVGTWLAHRFAGQWWPLAVFYTCLAALSLGGVIALDARRHRRADVADAVLTR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=434, Percent_Identity=35.7142857142857, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790550, Length=393, Percent_Identity=31.8066157760814, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=33.7995337995338, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1788942, Length=384, Percent_Identity=30.2083333333333, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI87082231, Length=439, Percent_Identity=22.0956719817768, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082404, Length=393, Percent_Identity=20.8651399491094, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49001; Mature: 48870

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNPDDSAGADIRVRRNALTRSIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALV
CCCCCCCCCCCEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FGELFFPSHDPFTSTMASFATFAVGFFARPLGGLFFGHLGDRIGRKKALMATLALMGAAT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCVGLLPTYERVGLLAPVLLVLLRIVQGIAVGGEWGGAVLMAGEHAPYGRRTFFASFAQL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
GSPAGLILSLVAFRAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEK
CCCHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHH
AMSRTVARPLAEVLRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHCHHHHHHHHH
LFIVAIIQFVTQPIAAWLGGKLGDARFLKLAALFAMASPYPMFALVQTGRLAPMVIGIAI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHH
ATVFLAGFYSVIAGFVSGAFPTRVRYSAISISYQMCGAIAGGLTPLVGTWLAHRFAGQWW
HHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCH
PLAVFYTCLAALSLGGVIALDARRHRRADVADAVLTR
HHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNPDDSAGADIRVRRNALTRSIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALV
CCCCCCCCCCEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FGELFFPSHDPFTSTMASFATFAVGFFARPLGGLFFGHLGDRIGRKKALMATLALMGAAT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCVGLLPTYERVGLLAPVLLVLLRIVQGIAVGGEWGGAVLMAGEHAPYGRRTFFASFAQL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
GSPAGLILSLVAFRAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEK
CCCHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHH
AMSRTVARPLAEVLRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHCHHHHHHHHH
LFIVAIIQFVTQPIAAWLGGKLGDARFLKLAALFAMASPYPMFALVQTGRLAPMVIGIAI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHH
ATVFLAGFYSVIAGFVSGAFPTRVRYSAISISYQMCGAIAGGLTPLVGTWLAHRFAGQWW
HHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCH
PLAVFYTCLAALSLGGVIALDARRHRRADVADAVLTR
HHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]