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The map label for this gene is emrA [H]

Identifier: 126443286

GI number: 126443286

Start: 1858944

End: 1860197

Strand: Reverse

Name: emrA [H]

Synonym: BURPS668_A1914

Alternate gene names: 126443286

Gene position: 1860197-1858944 (Counterclockwise)

Preceding gene: 126445040

Following gene: 126444168

Centisome position: 59.48

GC content: 73.52

Gene sequence:

>1254_bases
TTGAACGACACCGCCACCGACACTGCGCGCGCGAAGGCGCCCACCGATCCGGCCGTTTCCGACGGCGCGCCCGCGAGCGG
GCACGGCTTGCCTGCGTCGCCCGAAACCGCCGCGACGCTTGCCGCGCGCCGGGCCACGCGCTGCCGCCGCTTCGCATGGT
TCTTCGGAGTGCTGGCGATCGTCGCGCTGGGCGCGGCGCTCTACTGGTTCATCGTGGGCCGCTTCAGCGAAGAGACGGAC
GATGCGTACGTGGCGGGCAACGTCGTGCAGATCGCCGCGCAGATCCAGGGGACGGTGACCGACGTGCTGGTGGCCGACAC
GCAGCAGGTGAAGGCGGGGCAGGCGCTGGTGCGGCTCGACGACGCGGAGGCGGCGGCGGCGTTCGCGCAGGCGCAGGCGC
AGCTCGCGCAGGCGGTGCGGCAGGTGGCGAACACGCGGCTGTCGATGCAGACGTACGAGCAGACGGTGAAGGCGCGCGAG
GCGGACCTGAAGCTCGCGCAGCAGGCGTATCGGGCGCGGGCGGGGGCGTCGGTGGAGGTGGTGGCGCCGGAGGAGCTGGC
GCGGGCGAAGTCGTCGCTGGCGAACGCGCAGGCGGCGCTGGCGGGAGCGCAGGCGCAACGGGAGGCGGCGCGCGCGCTGG
GCAGCGAGCGGCCGGTCGGGCAGAACCCGGCGGTGCAGCAGGCGGCCGCGCAGTTCAAGCTGGCGTACCGGAACCTGAAG
CGCACGACGATCGTGTCGCCGGTGGACGGGACGGTCGGGCAGCGGTCGGTGCAGATCGGGCAGCAGGTGGGGCCGGGGGT
GCCGCTGATGTCGGTGGTGCAGTTGCGGCAGGTGTGGGTGGAGGCGAACTTCAAGGAAGGGCAGTTGCGGCACATGCGGG
TGGGCCAGCCGGTGCGGCTCGAATCGGACCTGTACGGCGCGCGGGTGACGTATCACGGCCGGGTGGAGGGTTTCTCGGCG
GGCACGGGCAGCGCGTTCTCGATGCTGCCGTCGCAGAACGCGGCGGGGAACTGGATCAAGGTGGTGCAGCGCCTGCCGGT
GGTGATCTCGCTGGAGCCGTCGGAGCTGGCGGCGCACCCGTTGCGGGTGGGGCTGTCGATGCGCGCGACGGTGCAGACGA
AGGCGCGCGGCGGGCATCTGCTGGACGGCGACGCGCCGCTGCCGGCGCAGCGCACGCGGGTGCACGAAATGCACGCGGGC
GAGGCGGAGGCGGCGGCCGAGGCGGTGATCCGCGCGAACGACGGTGGGCAGTGA

Upstream 100 bases:

>100_bases
CGCCGCCGAGCATCGAAGCACCCCGCGCCGCGCCGCAGCGGCGCCCGGGCCCGTCCGATCCGCGCCTGCCGGCGCCACCG
ACATCACGCACGCCACCATC

Downstream 100 bases:

>100_bases
CGGGCCGGGGGGATCGCGGGGGGCGCGGTGTGGCTGGGACGGGCGCAGGCGTCGATCGAGTCGGGCGGGGCGCTGGATCG
ATCGGCGTGATTGCGCTGCT

Product: multidrug resistance efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNDTATDTARAKAPTDPAVSDGAPASGHGLPASPETAATLAARRATRCRRFAWFFGVLAIVALGAALYWFIVGRFSEETD
DAYVAGNVVQIAAQIQGTVTDVLVADTQQVKAGQALVRLDDAEAAAAFAQAQAQLAQAVRQVANTRLSMQTYEQTVKARE
ADLKLAQQAYRARAGASVEVVAPEELARAKSSLANAQAALAGAQAQREAARALGSERPVGQNPAVQQAAAQFKLAYRNLK
RTTIVSPVDGTVGQRSVQIGQQVGPGVPLMSVVQLRQVWVEANFKEGQLRHMRVGQPVRLESDLYGARVTYHGRVEGFSA
GTGSAFSMLPSQNAAGNWIKVVQRLPVVISLEPSELAAHPLRVGLSMRATVQTKARGGHLLDGDAPLPAQRTRVHEMHAG
EAEAAAEAVIRANDGGQ

Sequences:

>Translated_417_residues
MNDTATDTARAKAPTDPAVSDGAPASGHGLPASPETAATLAARRATRCRRFAWFFGVLAIVALGAALYWFIVGRFSEETD
DAYVAGNVVQIAAQIQGTVTDVLVADTQQVKAGQALVRLDDAEAAAAFAQAQAQLAQAVRQVANTRLSMQTYEQTVKARE
ADLKLAQQAYRARAGASVEVVAPEELARAKSSLANAQAALAGAQAQREAARALGSERPVGQNPAVQQAAAQFKLAYRNLK
RTTIVSPVDGTVGQRSVQIGQQVGPGVPLMSVVQLRQVWVEANFKEGQLRHMRVGQPVRLESDLYGARVTYHGRVEGFSA
GTGSAFSMLPSQNAAGNWIKVVQRLPVVISLEPSELAAHPLRVGLSMRATVQTKARGGHLLDGDAPLPAQRTRVHEMHAG
EAEAAAEAVIRANDGGQ
>Mature_417_residues
MNDTATDTARAKAPTDPAVSDGAPASGHGLPASPETAATLAARRATRCRRFAWFFGVLAIVALGAALYWFIVGRFSEETD
DAYVAGNVVQIAAQIQGTVTDVLVADTQQVKAGQALVRLDDAEAAAAFAQAQAQLAQAVRQVANTRLSMQTYEQTVKARE
ADLKLAQQAYRARAGASVEVVAPEELARAKSSLANAQAALAGAQAQREAARALGSERPVGQNPAVQQAAAQFKLAYRNLK
RTTIVSPVDGTVGQRSVQIGQQVGPGVPLMSVVQLRQVWVEANFKEGQLRHMRVGQPVRLESDLYGARVTYHGRVEGFSA
GTGSAFSMLPSQNAAGNWIKVVQRLPVVISLEPSELAAHPLRVGLSMRATVQTKARGGHLLDGDAPLPAQRTRVHEMHAG
EAEAAAEAVIRANDGGQ

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=360, Percent_Identity=41.9444444444444, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1788711, Length=333, Percent_Identity=45.045045045045, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1790519, Length=322, Percent_Identity=28.8819875776398, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI87081951, Length=306, Percent_Identity=25.1633986928105, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789637, Length=309, Percent_Identity=27.5080906148867, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787013, Length=326, Percent_Identity=28.2208588957055, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44059; Mature: 44059

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTATDTARAKAPTDPAVSDGAPASGHGLPASPETAATLAARRATRCRRFAWFFGVLAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VALGAALYWFIVGRFSEETDDAYVAGNVVQIAAQIQGTVTDVLVADTQQVKAGQALVRLD
HHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEC
DAEAAAAFAQAQAQLAQAVRQVANTRLSMQTYEQTVKAREADLKLAQQAYRARAGASVEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VAPEELARAKSSLANAQAALAGAQAQREAARALGSERPVGQNPAVQQAAAQFKLAYRNLK
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RTTIVSPVDGTVGQRSVQIGQQVGPGVPLMSVVQLRQVWVEANFKEGQLRHMRVGQPVRL
HHEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEHCCCCCCEEE
ESDLYGARVTYHGRVEGFSAGTGSAFSMLPSQNAAGNWIKVVQRLPVVISLEPSELAAHP
CCCCCCCEEEEECCCCCCCCCCCCHHEECCCCCCCCHHHHHHHHCCEEEEECHHHHHHHH
LRVGLSMRATVQTKARGGHLLDGDAPLPAQRTRVHEMHAGEAEAAAEAVIRANDGGQ
HHHCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHEECCCCCC
>Mature Secondary Structure
MNDTATDTARAKAPTDPAVSDGAPASGHGLPASPETAATLAARRATRCRRFAWFFGVLAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VALGAALYWFIVGRFSEETDDAYVAGNVVQIAAQIQGTVTDVLVADTQQVKAGQALVRLD
HHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEC
DAEAAAAFAQAQAQLAQAVRQVANTRLSMQTYEQTVKAREADLKLAQQAYRARAGASVEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VAPEELARAKSSLANAQAALAGAQAQREAARALGSERPVGQNPAVQQAAAQFKLAYRNLK
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RTTIVSPVDGTVGQRSVQIGQQVGPGVPLMSVVQLRQVWVEANFKEGQLRHMRVGQPVRL
HHEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEHCCCCCCEEE
ESDLYGARVTYHGRVEGFSAGTGSAFSMLPSQNAAGNWIKVVQRLPVVISLEPSELAAHP
CCCCCCCEEEEECCCCCCCCCCCCHHEECCCCCCCCHHHHHHHHCCEEEEECHHHHHHHH
LRVGLSMRATVQTKARGGHLLDGDAPLPAQRTRVHEMHAGEAEAAAEAVIRANDGGQ
HHHCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]