Click here to switch to the map view.

The map label for this gene is narH [H]

Identifier: 126442732

GI number: 126442732

Start: 1555536

End: 1557065

Strand: Reverse

Name: narH [H]

Synonym: BURPS668_A1629

Alternate gene names: 126442732

Gene position: 1557065-1555536 (Counterclockwise)

Preceding gene: 126444060

Following gene: 126442632

Centisome position: 49.79

GC content: 65.56

Gene sequence:

>1530_bases
ATGAAGATCCGCGCGCAGATCGCGATGGTGCTCAACCTGGACAAGTGCATCGGCTGCCACACGTGCTCGGTCACGTGCAA
GAACGTGTGGACGAGCCGCGAAGGCATGGAATACGCGTGGTTCAACAACGTCGAGACGAAGCCGGGCATCGGCTATCCGA
AGGACTGGGAGAACCAGGCGCGCTGGCGCGGCGGCTGGAAGCGGCGCGCGGACGGCCGGATCGAGCCGCTGCTCGGCGGC
AAGTGGCGGCTGCTCGCGCAGATCTTCGCGAATCCGCATCTGCCTGAAATCGACGACTACTACGAGCCGTTCACGTTCGA
CTACGCGCATTTGCAGGAGGCGGGCGACACGAAGGCGATGCCGGTGGCGCGCCCGCGCTCGCTCGTGAGCGGCGAGCGGC
TCGAGAAGATCGAGTGGGGGCCGAACTGGGAGGAAATCCTCGGCGGCGAATTCGACAAACGCTCGAAGGATTACAACTTC
GAGCACGTGCAGAAGGACATCTACGGGCAGTTCGAGAACACGTTCATGATGTACCTGCCGCGGCTGTGCGAGCACTGCCT
GAACCCGGCGTGCGTTGCCGCGTGCCCGTCGGGCTCGATCTACAAGCGCGAGGAAGACGGAATCGTGCTGATCGATCAGG
ACAAGTGCCGCGGCTGGCGGATGTGCGTGTCCGGCTGCCCGTACAAGAAGATCTACTACAACTGGCAGAGCGGCAAGGCG
GAAAAGTGCATCTTCTGCTACCCGCGCATCGAGGCGGGCCAGCCCACCGTGTGCTCGGAAACCTGCGTCGGGCGCATCCG
CTATCTCGGCGTGCTGCTGTACGACGCCGATCGGATCGGCGAAGCGGCGAGCGTCGAGCGCGAAACCGATCTGTACGAAG
CGCAGTTGTCGCTCTTTCTCGATCCGAACGATCCGGCGATCGCCGCGCACGCCGAGCGCGACGGCGTGCCGGCCGCATGG
CTCGACGCGGCGCGGCGCTCGCCCGTCTACAAGATGGCGATCGACTGGAAGATCGCGTTCCCGCTGCATCCGGAGTACCG
GACGCTGCCGATGGTCTGGTACGTGCCGCCGCTCTCGCCGATCAACGCGGCCGCGAACAGCGGCGCGCTCGGGATGAACG
GCTATCTGCCCGATGTCGAATCGCTGCGCATTCCGCTGCGCTATCTGGCGAACCTGCTGAGCGCGGGCGACGAAGCGCCC
GTGAAGCGCGCGCTCGAGCGGCTGCTCGCGATGCGCGCGTTCATGCGCGCGCGGCACGTCGATCGCGTCGACGCGCCGGG
CGTGCTGAAGCAGGCGGGCCTGTCGGTCGCGCAGGTCGACGAGATGTACCGCTATCTGGCGATCGCGAACTACGAGGATC
GCTTCGTGATCCCGACGGCCCATCGCGAATACGCGGAGGACGCGTTCGATCTGCGCGCGTCGTGCGGCTTCTCGTTCGGC
AACGGCTGCTCGGGCGGCACGTCCGAGACGAGTCTCTTCGGGATGAAGCAAGGGCAGCGAACGATTCCGATCAAGGAGGT
GGAGCAATGA

Upstream 100 bases:

>100_bases
GGATCGATTGGCTCGACGGCGAAAACGAAAGCGCGCGCGCGCCGAACGTCTCGACATCGGGCGCCGACGACGAACGCAAT
GCCGACGCAGGAGAAACGCG

Downstream 100 bases:

>100_bases
GCGACGCACCAGCCGCGCCGCGCGGCGCCGTGTACTCGGCGCTGTCGGCGCTGCTCGATTATCCGGAGGACGCGCTCGTC
GATGCGCTGCCCGCGATCGA

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQARWRGGWKRRADGRIEPLLGG
KWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQEAGDTKAMPVARPRSLVSGERLEKIEWGPNWEEILGGEFDKRSKDYNF
EHVQKDIYGQFENTFMMYLPRLCEHCLNPACVAACPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQSGKA
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIGEAASVERETDLYEAQLSLFLDPNDPAIAAHAERDGVPAAW
LDAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPINAAANSGALGMNGYLPDVESLRIPLRYLANLLSAGDEAP
VKRALERLLAMRAFMRARHVDRVDAPGVLKQAGLSVAQVDEMYRYLAIANYEDRFVIPTAHREYAEDAFDLRASCGFSFG
NGCSGGTSETSLFGMKQGQRTIPIKEVEQ

Sequences:

>Translated_509_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQARWRGGWKRRADGRIEPLLGG
KWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQEAGDTKAMPVARPRSLVSGERLEKIEWGPNWEEILGGEFDKRSKDYNF
EHVQKDIYGQFENTFMMYLPRLCEHCLNPACVAACPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQSGKA
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIGEAASVERETDLYEAQLSLFLDPNDPAIAAHAERDGVPAAW
LDAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPINAAANSGALGMNGYLPDVESLRIPLRYLANLLSAGDEAP
VKRALERLLAMRAFMRARHVDRVDAPGVLKQAGLSVAQVDEMYRYLAIANYEDRFVIPTAHREYAEDAFDLRASCGFSFG
NGCSGGTSETSLFGMKQGQRTIPIKEVEQ
>Mature_509_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQARWRGGWKRRADGRIEPLLGG
KWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQEAGDTKAMPVARPRSLVSGERLEKIEWGPNWEEILGGEFDKRSKDYNF
EHVQKDIYGQFENTFMMYLPRLCEHCLNPACVAACPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQSGKA
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIGEAASVERETDLYEAQLSLFLDPNDPAIAAHAERDGVPAAW
LDAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPINAAANSGALGMNGYLPDVESLRIPLRYLANLLSAGDEAP
VKRALERLLAMRAFMRARHVDRVDAPGVLKQAGLSVAQVDEMYRYLAIANYEDRFVIPTAHREYAEDAFDLRASCGFSFG
NGCSGGTSETSLFGMKQGQRTIPIKEVEQ

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=494, Percent_Identity=71.8623481781377, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=498, Percent_Identity=70.0803212851406, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=36.3636363636364, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=36.3636363636364, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI2367345, Length=142, Percent_Identity=27.4647887323944, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789370, Length=106, Percent_Identity=37.7358490566038, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=34.4444444444444, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57699; Mature: 57699

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQA
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCH
RWRGGWKRRADGRIEPLLGGKWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQEAGDTKAM
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCC
PVARPRSLVSGERLEKIEWGPNWEEILGGEFDKRSKDYNFEHVQKDIYGQFENTFMMYLP
CCCCCHHHHCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVAACPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQSGKA
HHHHHHCCCCEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECCCCC
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIGEAASVERETDLYEAQLSLFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECHHHCCCHHCCHHHHHHEEEEEEEEE
DPNDPAIAAHAERDGVPAAWLDAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCH
INAAANSGALGMNGYLPDVESLRIPLRYLANLLSAGDEAPVKRALERLLAMRAFMRARHV
HHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DRVDAPGVLKQAGLSVAQVDEMYRYLAIANYEDRFVIPTAHREYAEDAFDLRASCGFSFG
CCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCC
NGCSGGTSETSLFGMKQGQRTIPIKEVEQ
CCCCCCCCCCEEEECCCCCCCCCHHHCCC
>Mature Secondary Structure
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVETKPGIGYPKDWENQA
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCH
RWRGGWKRRADGRIEPLLGGKWRLLAQIFANPHLPEIDDYYEPFTFDYAHLQEAGDTKAM
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCC
PVARPRSLVSGERLEKIEWGPNWEEILGGEFDKRSKDYNFEHVQKDIYGQFENTFMMYLP
CCCCCHHHHCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVAACPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQSGKA
HHHHHHCCCCEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECCCCC
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIGEAASVERETDLYEAQLSLFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECHHHCCCHHCCHHHHHHEEEEEEEEE
DPNDPAIAAHAERDGVPAAWLDAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCH
INAAANSGALGMNGYLPDVESLRIPLRYLANLLSAGDEAPVKRALERLLAMRAFMRARHV
HHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
DRVDAPGVLKQAGLSVAQVDEMYRYLAIANYEDRFVIPTAHREYAEDAFDLRASCGFSFG
CCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCC
NGCSGGTSETSLFGMKQGQRTIPIKEVEQ
CCCCCCCCCCEEEECCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]