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The map label for this gene is hycE [H]

Identifier: 126442362

GI number: 126442362

Start: 1541369

End: 1543093

Strand: Reverse

Name: hycE [H]

Synonym: BURPS668_A1613

Alternate gene names: 126442362

Gene position: 1543093-1541369 (Counterclockwise)

Preceding gene: 126443859

Following gene: 126443278

Centisome position: 49.34

GC content: 72.7

Gene sequence:

>1725_bases
ATGCGAATCGACGCATTGCGAATGTCCGGTCAGACGCGCATCGCGAGCGCGGCGGGGCAGCCGCGCGCCGTGTTCGCGTC
GGTCGACGCCGACGCATGGACGCGCGCCGCCGAGACGATCCGGACGGCCGGCGGGCGGCTCGTCACGCTGTGGGGCGGCG
AGCCCGCGGCCGGGCGCTACGAGGTCTGCGCCGCGTACGAGCTGGACGACGGGCTGTTGTGGCTGCGCCTGCCGGTCGAG
CCGGCGGCGAACGTGGGGCGGGAAGCCGGCGACACGAGCAACGCGGGCAACGCGAGCGACGCGAGCGACGCGGACGGCGT
GCGGCAACGCGGCGAGGCCGGCAGCTATCCGGATCTGTCGGCGATCTTCGCGAGCGCGACGCGGATGCAACGGGCGCTTC
ACGATCTCGTCGGCCTGCGCGCGCGCGGCGCGCACGACACGCGGCCGTGGCTCGCCCACGGCGCATGGCCGCGCGATTAC
CACCCGCTGCGCAGGCGCGCGACGGGCGGCGAGCGCTTCGAGTACGCGACGGCCGACTATCCGTTCGTGCCGGTCGCGGG
CGACGGCGTGCATGAGATCGCGGTCGGGCCGATTCATGCGGGCGTGATCGAGCCCGGGCATTTCCGCTTTTCCGTCGTCG
GCGAAAAGGTGCTGCGGCTCGAGGAGCGGCTTGGCTACGCGCATCGCGGCGTCGAACGGCTCTTCGAGCGCGCGGATGCG
CTCGCGGGCAGCCGGCTCGCCGCGCGCATCGCCGGCGATTCGACGGTCGCGTTCGCGTGGGCGTACTGCATGGCGCTCGA
ACAGGCGTTGAGAATCCGCGTGCCGGACCGCGCGCTGCGGCTGCGCGCGCTGCTGCTCGAGCGCGAGCGCGTCGCGAACC
ATCTGGGCGACCTCGGCGCGCTCGGCAACGACGCGGGTTTTGCCGTGGGCCTCGCGCATTTCTCGCGGCTCAAGGAAGAC
TGGCTGCGGATGCAGGATCGGATCTTCGGGCATCGCTACCTGATGGACGCGATCGTGCCGGGCGGCGTCGCGCGCGACAT
CGCGGCGGACGACGCCGCGGCGATCGTCGCGCAGTGCGACCGGATCGAGCGTGAAGTGCGCGCGATGCAAACGATCTACG
ACGATCAATCCGGCCTGCAGGACCGCTTCGCCGGCACCGGGCGGCTCGACGCGCAGGCGGTCGCGCATTTCGGCGTATGC
GGGCTCGCCGCCCGGGCGAGCGGGCAGCCGCGCGACGTGCGCGCGGACCATCGGCTGGCGCCATACGACGCGCTGCGCAT
CGACGTCAGCCGCGACGCGCGCGGCGACGTCGCGGCGCGCGTCGCGGTGCGCTTCGCCGAAACGTACGAGTCGATGCGCG
CGATCCGCGCGCTGCTCGCGGATTTGCCGGACGACGGGCTCGCCGCGGAGATCGCGCCCGGCGCGAATGTTCCGGGCGCG
CGCGCCGATGCGGGCGGAGCAGGCGAAGGGGAAGAGGGGGGCGACGCCGCATTCTGCCCGGGCGACGCGCGGCGGCGCGG
CATCGGCTGGATCGAAGGCTGGCGCGGGCCCGTGTTCGTCGCGCTCGAACTCGATGCGCGCGGCGCCGTCGCGCGCTGCC
ATTGCCATGATCCGTCATGGCACAACTGGCCCGCGCTCGAGCATGCGATCATCGGCAACATCGTCGCGGACTTCCCGTTG
ATCAACAAGTCGTTCAACCTGAACTACGCGGGGCACGACCTGTAA

Upstream 100 bases:

>100_bases
GTTCGTTCACCTCGCACTCGGCTTGATGCTCGGGCTTTACGTGCCGCCGTATCTGGCCGCCTGGTATCGGCAGGCGGCGG
CGATGATCGCGGGGTAGCCG

Downstream 100 bases:

>100_bases
TGTGGCAACTCATCAAACAGATCGCGCGCACCGGCCGGCCTAACGAACCCGCGCCGGCGGGCGAAGACGCCTGGCGGCGG
GATGCGCAGTGCATTCGGCA

Product: putative hydrogenase subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MRIDALRMSGQTRIASAAGQPRAVFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRYEVCAAYELDDGLLWLRLPVE
PAANVGREAGDTSNAGNASDASDADGVRQRGEAGSYPDLSAIFASATRMQRALHDLVGLRARGAHDTRPWLAHGAWPRDY
HPLRRRATGGERFEYATADYPFVPVAGDGVHEIAVGPIHAGVIEPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADA
LAGSRLAARIAGDSTVAFAWAYCMALEQALRIRVPDRALRLRALLLERERVANHLGDLGALGNDAGFAVGLAHFSRLKED
WLRMQDRIFGHRYLMDAIVPGGVARDIAADDAAAIVAQCDRIEREVRAMQTIYDDQSGLQDRFAGTGRLDAQAVAHFGVC
GLAARASGQPRDVRADHRLAPYDALRIDVSRDARGDVAARVAVRFAETYESMRAIRALLADLPDDGLAAEIAPGANVPGA
RADAGGAGEGEEGGDAAFCPGDARRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSWHNWPALEHAIIGNIVADFPL
INKSFNLNYAGHDL

Sequences:

>Translated_574_residues
MRIDALRMSGQTRIASAAGQPRAVFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRYEVCAAYELDDGLLWLRLPVE
PAANVGREAGDTSNAGNASDASDADGVRQRGEAGSYPDLSAIFASATRMQRALHDLVGLRARGAHDTRPWLAHGAWPRDY
HPLRRRATGGERFEYATADYPFVPVAGDGVHEIAVGPIHAGVIEPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADA
LAGSRLAARIAGDSTVAFAWAYCMALEQALRIRVPDRALRLRALLLERERVANHLGDLGALGNDAGFAVGLAHFSRLKED
WLRMQDRIFGHRYLMDAIVPGGVARDIAADDAAAIVAQCDRIEREVRAMQTIYDDQSGLQDRFAGTGRLDAQAVAHFGVC
GLAARASGQPRDVRADHRLAPYDALRIDVSRDARGDVAARVAVRFAETYESMRAIRALLADLPDDGLAAEIAPGANVPGA
RADAGGAGEGEEGGDAAFCPGDARRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSWHNWPALEHAIIGNIVADFPL
INKSFNLNYAGHDL
>Mature_574_residues
MRIDALRMSGQTRIASAAGQPRAVFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRYEVCAAYELDDGLLWLRLPVE
PAANVGREAGDTSNAGNASDASDADGVRQRGEAGSYPDLSAIFASATRMQRALHDLVGLRARGAHDTRPWLAHGAWPRDY
HPLRRRATGGERFEYATADYPFVPVAGDGVHEIAVGPIHAGVIEPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADA
LAGSRLAARIAGDSTVAFAWAYCMALEQALRIRVPDRALRLRALLLERERVANHLGDLGALGNDAGFAVGLAHFSRLKED
WLRMQDRIFGHRYLMDAIVPGGVARDIAADDAAAIVAQCDRIEREVRAMQTIYDDQSGLQDRFAGTGRLDAQAVAHFGVC
GLAARASGQPRDVRADHRLAPYDALRIDVSRDARGDVAARVAVRFAETYESMRAIRALLADLPDDGLAAEIAPGANVPGA
RADAGGAGEGEEGGDAAFCPGDARRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSWHNWPALEHAIIGNIVADFPL
INKSFNLNYAGHDL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=367, Percent_Identity=24.5231607629428, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI260898743, Length=367, Percent_Identity=24.5231607629428, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1789076, Length=465, Percent_Identity=31.1827956989247, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788832, Length=466, Percent_Identity=29.6137339055794, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI145693162, Length=466, Percent_Identity=22.1030042918455, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17555284, Length=371, Percent_Identity=23.4501347708895, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17568379, Length=373, Percent_Identity=23.8605898123324, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221459469, Length=271, Percent_Identity=27.3062730627306, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24638644, Length=346, Percent_Identity=25.1445086705202, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 61878; Mature: 61878

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDALRMSGQTRIASAAGQPRAVFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRY
CCCCCEECCCCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCE
EVCAAYELDDGLLWLRLPVEPAANVGREAGDTSNAGNASDASDADGVRQRGEAGSYPDLS
EEEEEEEECCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHH
AIFASATRMQRALHDLVGLRARGAHDTRPWLAHGAWPRDYHPLRRRATGGERFEYATADY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHCCCCCCEEEECCCC
PFVPVAGDGVHEIAVGPIHAGVIEPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADA
CEEEECCCCCCEEEECCEECCCCCCCCEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LAGSRLAARIAGDSTVAFAWAYCMALEQALRIRVPDRALRLRALLLERERVANHLGDLGA
HHCCHHEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
LGNDAGFAVGLAHFSRLKEDWLRMQDRIFGHRYLMDAIVPGGVARDIAADDAAAIVAQCD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHH
RIEREVRAMQTIYDDQSGLQDRFAGTGRLDAQAVAHFGVCGLAARASGQPRDVRADHRLA
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PYDALRIDVSRDARGDVAARVAVRFAETYESMRAIRALLADLPDDGLAAEIAPGANVPGA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
RADAGGAGEGEEGGDAAFCPGDARRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSW
CCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCC
HNWPALEHAIIGNIVADFPLINKSFNLNYAGHDL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIDALRMSGQTRIASAAGQPRAVFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRY
CCCCCEECCCCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCE
EVCAAYELDDGLLWLRLPVEPAANVGREAGDTSNAGNASDASDADGVRQRGEAGSYPDLS
EEEEEEEECCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHH
AIFASATRMQRALHDLVGLRARGAHDTRPWLAHGAWPRDYHPLRRRATGGERFEYATADY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHCCCCCCEEEECCCC
PFVPVAGDGVHEIAVGPIHAGVIEPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADA
CEEEECCCCCCEEEECCEECCCCCCCCEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LAGSRLAARIAGDSTVAFAWAYCMALEQALRIRVPDRALRLRALLLERERVANHLGDLGA
HHCCHHEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
LGNDAGFAVGLAHFSRLKEDWLRMQDRIFGHRYLMDAIVPGGVARDIAADDAAAIVAQCD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHH
RIEREVRAMQTIYDDQSGLQDRFAGTGRLDAQAVAHFGVCGLAARASGQPRDVRADHRLA
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PYDALRIDVSRDARGDVAARVAVRFAETYESMRAIRALLADLPDDGLAAEIAPGANVPGA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
RADAGGAGEGEEGGDAAFCPGDARRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSW
CCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCC
HNWPALEHAIIGNIVADFPLINKSFNLNYAGHDL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]