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The map label for this gene is phaZ1 [H]

Identifier: 126442346

GI number: 126442346

Start: 2469816

End: 2470964

Strand: Reverse

Name: phaZ1 [H]

Synonym: BURPS668_A2562

Alternate gene names: 126442346

Gene position: 2470964-2469816 (Counterclockwise)

Preceding gene: 126443051

Following gene: 126443044

Centisome position: 79.01

GC content: 70.06

Gene sequence:

>1149_bases
ATGACCAAAAGTCTGACGAAATTCTGGCTGGGCGGTGTGAAACGCATGCTGCACATGCCGAACGCGCGGACGTCGGCGTC
CGCCCTGAAGGCTGCCCAAAAGATGACGGCCGACTGGCCGTTCGCCGAAACGATGGCCGCGGCGGCGGCCGCTTCAGTTG
CCTCCGTCGCGGCGTCCGCCGACACGGTACCGCCCGCCGCGCGCGAATCGCGCGTACGGCCGCGCGCGGCCGCTTGGGCG
GGCGGCGAATGGATCCGCGCCGATCACCCGTTGCCGCCCGCGTTCGGGCGCTTCGTCAAGCATCTCGAATACGGGCTGTA
CGTGCCGTCCGGCGCGAAGACGCTCGGTTTGCCGCTCGTCGTGATGCTGCACGGCTGCAAGCAGGACATGAATCAGTTCT
CGCAAGGCACGCGGATGAACTTGCTCGCGGACCGATACGGCTTCGCCGTGCTCTACCCGGAGCAGTCGCTCAGCGCGCAC
GCGCACGGCTGCTGGCACTGGTACGAGGACACCGTGCACGGCGGGCGCGGCGAAGCGCAGGCGGTCGTCGCGCTCGTCGA
CGCGCTCGTCGCCGAGCGCGGCTTTGACGCATCGCGCGTGTATGCGGCCGGCATGTCGGCGGGCGCGGGGCTCGTATCGT
TGCTCGCGCTGCATTTCCCGCGGCGCTTCGCGGCCGTGGCGCTCCATTCGGGGCCGGCGTTCGGCGATGCGCATTCCGGG
ATCACCGCGATGGACGTGATGCGCCGCGGCCTGCACCGCGATCCCGCCGCCGTTGTCGACGCGCTCGTCGAGCCGGGCGC
GCATCCGGGAATGCCGGCCTTCATCGTGCACGGCGACGACGATCGCGTTGTCGTGCCGAAGAACGCGGACGAACTCGCCG
TTCAGTTCCTGCGGCTGAACGGTCTTGCCGACGCCGACGGTGAACCGGCGGGCGTCGAGCGTTTCGAGACGCGCGCAGCC
GACGTGCGCACGCTCGACTATCGCCGCGACGGCGAATCGGTCGTGCGCCTCTGCCGGGTCCACGGCCTCGCGCATGCATG
GGCGGGCGGCGACGACAGCGTGCCGTTCCACTCGGCCGCCGGCCCGGATGCGAGCGAGCTGATCTGGTCTTTCTTCGCGT
CGCGTGAGCGAGCGGCTGTCGCGTCGTAA

Upstream 100 bases:

>100_bases
CGGGATAAATCCCAGAAACAGTGTTATTGTGCATTGCACAAACCAAAACGATTGCACCCACTGAGGTGTCCCTCATTCTT
CTAACTGCGAGCCCCGACCG

Downstream 100 bases:

>100_bases
CGACGCACGCTCGGGAACGGCTGGCGTTGGTTCAGGGTTTTCCCTATAATGCGGGTAAATACCTAAGAGAAAACCCTTGG
ATTGGAGTGACCGATCATGT

Product: depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTKSLTKFWLGGVKRMLHMPNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWA
GGEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAH
AHGCWHWYEDTVHGGRGEAQAVVALVDALVAERGFDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSG
ITAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAA
DVRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERAAVAS

Sequences:

>Translated_382_residues
MTKSLTKFWLGGVKRMLHMPNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWA
GGEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAH
AHGCWHWYEDTVHGGRGEAQAVVALVDALVAERGFDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSG
ITAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAA
DVRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERAAVAS
>Mature_381_residues
TKSLTKFWLGGVKRMLHMPNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWAG
GEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAHA
HGCWHWYEDTVHGGRGEAQAVVALVDALVAERGFDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSGI
TAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAAD
VRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERAAVAS

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40743; Mature: 40611

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSLTKFWLGGVKRMLHMPNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASA
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
DTVPPAARESRVRPRAAAWAGGEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKTLGLPLV
CCCCCCHHHHCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHCEECCCCCCHHHHHHH
VMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGEAQ
HHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEECCHHCCCCCCHHHHHHHHHHCCCCCHHH
AVVALVDALVAERGFDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSG
HHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHEEEECCCCCCCCCCC
ITAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLN
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHC
GLADADGEPAGVERFETRAADVRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSVPFHSAA
CCCCCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GPDASELIWSFFASRERAAVAS
CCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKSLTKFWLGGVKRMLHMPNARTSASALKAAQKMTADWPFAETMAAAAAASVASVAASA
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
DTVPPAARESRVRPRAAAWAGGEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKTLGLPLV
CCCCCCHHHHCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHCEECCCCCCHHHHHHH
VMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGEAQ
HHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEECCHHCCCCCCHHHHHHHHHHCCCCCHHH
AVVALVDALVAERGFDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSG
HHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHEEEECCCCCCCCCCC
ITAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAFIVHGDDDRVVVPKNADELAVQFLRLN
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHC
GLADADGEPAGVERFETRAADVRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSVPFHSAA
CCCCCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GPDASELIWSFFASRERAAVAS
CCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]