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The map label for this gene is ubiB

Identifier: 126209438

GI number: 126209438

Start: 2211332

End: 2212966

Strand: Direct

Name: ubiB

Synonym: APL_1984

Alternate gene names: 126209438

Gene position: 2211332-2212966 (Clockwise)

Preceding gene: 126209437

Following gene: 126209439

Centisome position: 97.22

GC content: 43.79

Gene sequence:

>1635_bases
ATGACTTGTAAAAATACCCGTCGCCTTTATCAAATTATCACTACGTTTCTGCGTTACGGCATTGATGAAATCATTCCCGA
TATTCCGCTTACTCGCCACGCCCGTTTAGGACGTAAAGCTCTGTTTTGGGTGCGAAATCAGCACAAAGATCAGCCGTTCG
GCGTGCGTTTACGTTTAGCTTTGCAAGAGCTAGGGCCGGTGTGGATTAAACTCGGGCAAATGCTTTCTACTCGCCGTGAT
TTATTTGAGCCGGAATTAGCGGAGCAGCTTGCTTTATTACAGGACTCGGTTGAACCTTTTGACGGTAAATCAGCCCGTCA
AATTATTGAACAAGCTTTAGGCGGAAGCCTTGAGACTTGGTTTGACGAATTCGATGAACAAGCGCTTGCCTCCGCTTCGA
TTGCACAGGTTCACACGGCAAAATTTAATCAAAATCAACCGCTTGCCGGCAAAGATGTCGTGCTTAAAGTGATTCGTCCG
GATATTGAGCCGATTATCAAGGCGGATATTGCATTGATGTATCGTCTGGCAAGTTGGATTCCTCGCCTTTCGAATGATGC
GAAGCGTTTACGTGCGACCGAAGTGGTGCGTGAATACGAAAAAACGCTACTTGATGAGCTGGATTTGACCCGAGAAATGG
CAAATGCGATTCGCTTGCGCAATAACTTTGAAAACAGCGAAATGTTATATGTGCCGGAAATGTATCAGGATTTTTGTCAT
AAAAACGTGATTGTGATGGAGCGTATTTACGGTATTCCGGTATCGGATGTCGAAACGCTCAAAGCCAACGGTACGGATAT
GAAATTATTGGCGGAACGTGGCGTACAAGTGTTCTTCACTCAAGTATTTCGTGACAGCTTTTTCCATGCCGATATGCACG
CCGGTAATATTTTTGTTAATCCGAATCATCCGGAAAATCCGCAATATATCGGCATTGACTGCGGTATTGTCGGTACACTG
AATCAAAATGATAAACGCTATTTAGCGGAAAGTTTTGTTGCCTTTTTTAATCGAGATTATCGCCGCGTGGCGTTAATGCA
CGTGGAGTCGGGCTGGACACCGCCGGATACCGATATTGATGCATTTGAACAGGCTTTTCGTGAAGTGTGTGAGCCGATTT
TCGCCAAACCGTTATCGGAAATTTCTTTTGGTCACGTGTTACTGAATCTGTTTAATGTGGCACGTGAATTTAATATGGAA
GTGCAGCCGCAATTAGTATTGTTACAGAAGACATTACTTTATATTGAGGGTTTAGGCAGACAGGTTTATCCGCAGCTGGA
TTTATGGCAAACCGCAAAACCGTTTTTACAAAATTGGCTGAATGAACAAGTAGGGGTGAAAGCGATTTTACGTGATTTAA
AACAACGCGCGCCACAATTTAGAGAACATTTTGCTGAATTTCCGGAAGCGGTATTCAACGCTTTACAGCAACAGAAGCAG
ATTAATTTCCGTTTAGATGAGCTAAATAAAACCTTGCAAGCGCAAGGGCGGCAAAAATCCCATAATGTACGGAGTATCGT
GAGCGGAGTAATTATTCTCGGTGTGTTATGGCGATTTGACGATTTACCGTTATGGCTGAGTTGCGGTACGTTGGTTACGG
TGTTGTTAGTGTTATTGCTACAAAGAAAGTCGTAA

Upstream 100 bases:

>100_bases
TGCATCGCTTACAAGCGGTTAATTTTTACGATCAAGTTGCAGAGCTTGAACAGCAAGCGGTCGAATTTGAACGAAAATTT
GCAAAATTTGAGAAGAAATC

Downstream 100 bases:

>100_bases
TAAATGACGTGTTTATATTGGTCATATTATTAAAATAGCATATAATGCGAAAAATCATTTTTAAACCTAAAAACAAAAAA
GAGGAGCCTGTATGGGTGGC

Product: ubiquinone biosynthesis protein ubiB

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTCKNTRRLYQIITTFLRYGIDEIIPDIPLTRHARLGRKALFWVRNQHKDQPFGVRLRLALQELGPVWIKLGQMLSTRRD
LFEPELAEQLALLQDSVEPFDGKSARQIIEQALGGSLETWFDEFDEQALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
DIEPIIKADIALMYRLASWIPRLSNDAKRLRATEVVREYEKTLLDELDLTREMANAIRLRNNFENSEMLYVPEMYQDFCH
KNVIVMERIYGIPVSDVETLKANGTDMKLLAERGVQVFFTQVFRDSFFHADMHAGNIFVNPNHPENPQYIGIDCGIVGTL
NQNDKRYLAESFVAFFNRDYRRVALMHVESGWTPPDTDIDAFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAREFNME
VQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAILRDLKQRAPQFREHFAEFPEAVFNALQQQKQ
INFRLDELNKTLQAQGRQKSHNVRSIVSGVIILGVLWRFDDLPLWLSCGTLVTVLLVLLLQRKS

Sequences:

>Translated_544_residues
MTCKNTRRLYQIITTFLRYGIDEIIPDIPLTRHARLGRKALFWVRNQHKDQPFGVRLRLALQELGPVWIKLGQMLSTRRD
LFEPELAEQLALLQDSVEPFDGKSARQIIEQALGGSLETWFDEFDEQALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
DIEPIIKADIALMYRLASWIPRLSNDAKRLRATEVVREYEKTLLDELDLTREMANAIRLRNNFENSEMLYVPEMYQDFCH
KNVIVMERIYGIPVSDVETLKANGTDMKLLAERGVQVFFTQVFRDSFFHADMHAGNIFVNPNHPENPQYIGIDCGIVGTL
NQNDKRYLAESFVAFFNRDYRRVALMHVESGWTPPDTDIDAFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAREFNME
VQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAILRDLKQRAPQFREHFAEFPEAVFNALQQQKQ
INFRLDELNKTLQAQGRQKSHNVRSIVSGVIILGVLWRFDDLPLWLSCGTLVTVLLVLLLQRKS
>Mature_543_residues
TCKNTRRLYQIITTFLRYGIDEIIPDIPLTRHARLGRKALFWVRNQHKDQPFGVRLRLALQELGPVWIKLGQMLSTRRDL
FEPELAEQLALLQDSVEPFDGKSARQIIEQALGGSLETWFDEFDEQALASASIAQVHTAKFNQNQPLAGKDVVLKVIRPD
IEPIIKADIALMYRLASWIPRLSNDAKRLRATEVVREYEKTLLDELDLTREMANAIRLRNNFENSEMLYVPEMYQDFCHK
NVIVMERIYGIPVSDVETLKANGTDMKLLAERGVQVFFTQVFRDSFFHADMHAGNIFVNPNHPENPQYIGIDCGIVGTLN
QNDKRYLAESFVAFFNRDYRRVALMHVESGWTPPDTDIDAFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAREFNMEV
QPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAILRDLKQRAPQFREHFAEFPEAVFNALQQQKQI
NFRLDELNKTLQAQGRQKSHNVRSIVSGVIILGVLWRFDDLPLWLSCGTLVTVLLVLLLQRKS

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily

Homologues:

Organism=Homo sapiens, GI41393593, Length=275, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI40254938, Length=279, Percent_Identity=26.5232974910394, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI32261307, Length=335, Percent_Identity=24.1791044776119, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI217035081, Length=225, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI34147522, Length=193, Percent_Identity=27.979274611399, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI2367309, Length=541, Percent_Identity=63.9556377079482, Blast_Score=712, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559152, Length=280, Percent_Identity=25, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17555620, Length=536, Percent_Identity=21.2686567164179, Blast_Score=95, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6325148, Length=454, Percent_Identity=25.9911894273128, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323282, Length=291, Percent_Identity=24.7422680412371, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321319, Length=249, Percent_Identity=23.2931726907631, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI22024280, Length=256, Percent_Identity=29.296875, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24662575, Length=267, Percent_Identity=27.7153558052434, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIB_ACTP2 (A3N3S2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054663.1
- ProteinModelPortal:   A3N3S2
- STRING:   A3N3S2
- GeneID:   4849534
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1984
- eggNOG:   COG0661
- HOGENOM:   HBG593509
- OMA:   RIQRVVI
- PhylomeDB:   A3N3S2
- ProtClustDB:   CLSK870574
- BioCyc:   APLE416269:APL_1984-MONOMER
- HAMAP:   MF_00414
- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232
- TIGRFAMs:   TIGR01982

Pfam domain/function: PF03109 ABC1; SSF56112 Kinase_like

EC number: NA

Molecular weight: Translated: 62865; Mature: 62734

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ef318ec)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCKNTRRLYQIITTFLRYGIDEIIPDIPLTRHARLGRKALFWVRNQHKDQPFGVRLRLA
CCCCHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHCHHHHHHHHHCCCCCCCCHHHHHH
LQELGPVWIKLGQMLSTRRDLFEPELAEQLALLQDSVEPFDGKSARQIIEQALGGSLETW
HHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHH
FDEFDEQALASASIAQVHTAKFNQNQPLAGKDVVLKVIRPDIEPIIKADIALMYRLASWI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
PRLSNDAKRLRATEVVREYEKTLLDELDLTREMANAIRLRNNFENSEMLYVPEMYQDFCH
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHC
KNVIVMERIYGIPVSDVETLKANGTDMKLLAERGVQVFFTQVFRDSFFHADMHAGNIFVN
CCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEC
PNHPENPQYIGIDCGIVGTLNQNDKRYLAESFVAFFNRDYRRVALMHVESGWTPPDTDID
CCCCCCCCEEEEECCEEECCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCHH
AFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAREFNMEVQPQLVLLQKTLLYIEGLGR
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
QVYPQLDLWQTAKPFLQNWLNEQVGVKAILRDLKQRAPQFREHFAEFPEAVFNALQQQKQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
INFRLDELNKTLQAQGRQKSHNVRSIVSGVIILGVLWRFDDLPLWLSCGTLVTVLLVLLL
HCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QRKS
HCCH
>Mature Secondary Structure 
TCKNTRRLYQIITTFLRYGIDEIIPDIPLTRHARLGRKALFWVRNQHKDQPFGVRLRLA
CCCHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHCHHHHHHHHHCCCCCCCCHHHHHH
LQELGPVWIKLGQMLSTRRDLFEPELAEQLALLQDSVEPFDGKSARQIIEQALGGSLETW
HHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHH
FDEFDEQALASASIAQVHTAKFNQNQPLAGKDVVLKVIRPDIEPIIKADIALMYRLASWI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
PRLSNDAKRLRATEVVREYEKTLLDELDLTREMANAIRLRNNFENSEMLYVPEMYQDFCH
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHC
KNVIVMERIYGIPVSDVETLKANGTDMKLLAERGVQVFFTQVFRDSFFHADMHAGNIFVN
CCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEC
PNHPENPQYIGIDCGIVGTLNQNDKRYLAESFVAFFNRDYRRVALMHVESGWTPPDTDID
CCCCCCCCEEEEECCEEECCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCHH
AFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAREFNMEVQPQLVLLQKTLLYIEGLGR
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
QVYPQLDLWQTAKPFLQNWLNEQVGVKAILRDLKQRAPQFREHFAEFPEAVFNALQQQKQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
INFRLDELNKTLQAQGRQKSHNVRSIVSGVIILGVLWRFDDLPLWLSCGTLVTVLLVLLL
HCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QRKS
HCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA