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The map label for this gene is gph [C]

Identifier: 126209353

GI number: 126209353

Start: 2125286

End: 2125951

Strand: Reverse

Name: gph [C]

Synonym: APL_1893

Alternate gene names: 126209353

Gene position: 2125951-2125286 (Counterclockwise)

Preceding gene: 126209354

Following gene: 126209352

Centisome position: 93.47

GC content: 41.59

Gene sequence:

>666_bases
ATGACTGCAAAATATAAAGTAATCGGTTTTGATCTTGACGGTACCTTGGTAAATACTCTGCCGGATTTAACTTTGGTGGC
TAATTCGATGTTTGCCGAATACGGTTTACCAACTACGACGCAAGAGAAGGTCTTGACCTGGATTGGTAAAGGTGCGGATA
TTTTCTTTCAAAATGCGATTGCTTACACCGGACAAGTGTTTGACGCCCCAAAATTAGTGCGGTTACGTACCAGTTTCGAT
AAATATTATGCCGCTTACGTTTGCGAAGAAAGCGAGTTATATCCGAATGTAAAACAAACTTTAGAAACGTTGAGGGCACA
AGGCTATACGTTGGTGGTGATTACCAATAAACCGACCAAATTGGTCGAGCCGGTATTAAGTGCGTTCGGGATTTTTGAAT
TGTTCAGTGAATATTTAGGCGGACAATCTCTACCGAAAATCAAACCGCATCCGGATCCGATGTTGCATATCTGTGAGAAA
TTTGCGATTCAGCCAAGTGAAATGTTATTTGTCGGTGATTCGGAAAATGATGTGATTGCCGCCAAAGCCGCCGGTTGTGA
TGTGGTCGGTTTAACTTACGGTTATAACTATAACATACCAATCGAGCAATCAAATCCAACCTTCGTTACCGGCGAATTTG
CTGACGTATTGAAATTTGTTGGTTAA

Upstream 100 bases:

>100_bases
CAAAACATTCATAAGAACGTAAAAAGCCGTAACCTCCGATTGTTACGGCTTTTTTATTTGCTAAAATTAGCGGCAATTGA
ATTTAGATAAGGAAAAAATT

Downstream 100 bases:

>100_bases
TTCTACATATAACAAGCGGTCAAATTCCCTTAGTTTTTTGCAAAAAATTTCTGGAATTTGACCGCTTGTGCTTTTATCGA
ATGGCAATAAAGGCTAAGAT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTAKYKVIGFDLDGTLVNTLPDLTLVANSMFAEYGLPTTTQEKVLTWIGKGADIFFQNAIAYTGQVFDAPKLVRLRTSFD
KYYAAYVCEESELYPNVKQTLETLRAQGYTLVVITNKPTKLVEPVLSAFGIFELFSEYLGGQSLPKIKPHPDPMLHICEK
FAIQPSEMLFVGDSENDVIAAKAAGCDVVGLTYGYNYNIPIEQSNPTFVTGEFADVLKFVG

Sequences:

>Translated_221_residues
MTAKYKVIGFDLDGTLVNTLPDLTLVANSMFAEYGLPTTTQEKVLTWIGKGADIFFQNAIAYTGQVFDAPKLVRLRTSFD
KYYAAYVCEESELYPNVKQTLETLRAQGYTLVVITNKPTKLVEPVLSAFGIFELFSEYLGGQSLPKIKPHPDPMLHICEK
FAIQPSEMLFVGDSENDVIAAKAAGCDVVGLTYGYNYNIPIEQSNPTFVTGEFADVLKFVG
>Mature_220_residues
TAKYKVIGFDLDGTLVNTLPDLTLVANSMFAEYGLPTTTQEKVLTWIGKGADIFFQNAIAYTGQVFDAPKLVRLRTSFDK
YYAAYVCEESELYPNVKQTLETLRAQGYTLVVITNKPTKLVEPVLSAFGIFELFSEYLGGQSLPKIKPHPDPMLHICEKF
AIQPSEMLFVGDSENDVIAAKAAGCDVVGLTYGYNYNIPIEQSNPTFVTGEFADVLKFVG

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=233, Percent_Identity=42.4892703862661, Blast_Score=183, Evalue=9e-48,
Organism=Escherichia coli, GI1788021, Length=207, Percent_Identity=24.6376811594203, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR000150
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24438; Mature: 24307

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKYKVIGFDLDGTLVNTLPDLTLVANSMFAEYGLPTTTQEKVLTWIGKGADIFFQNAI
CCCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEHHHH
AYTGQVFDAPKLVRLRTSFDKYYAAYVCEESELYPNVKQTLETLRAQGYTLVVITNKPTK
HHCCCCCCCCHHEEEHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEEECCCHH
LVEPVLSAFGIFELFSEYLGGQSLPKIKPHPDPMLHICEKFAIQPSEMLFVGDSENDVIA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEE
AKAAGCDVVGLTYGYNYNIPIEQSNPTFVTGEFADVLKFVG
EECCCCCEEEEEECCEECCEEECCCCCEEECCHHHHHHHCC
>Mature Secondary Structure 
TAKYKVIGFDLDGTLVNTLPDLTLVANSMFAEYGLPTTTQEKVLTWIGKGADIFFQNAI
CCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEHHHH
AYTGQVFDAPKLVRLRTSFDKYYAAYVCEESELYPNVKQTLETLRAQGYTLVVITNKPTK
HHCCCCCCCCHHEEEHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEEECCCHH
LVEPVLSAFGIFELFSEYLGGQSLPKIKPHPDPMLHICEKFAIQPSEMLFVGDSENDVIA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEE
AKAAGCDVVGLTYGYNYNIPIEQSNPTFVTGEFADVLKFVG
EECCCCCEEEEEECCEECCEEECCCCCEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA