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The map label for this gene is yugJ [H]

Identifier: 126209321

GI number: 126209321

Start: 2090045

End: 2090944

Strand: Reverse

Name: yugJ [H]

Synonym: APL_1859

Alternate gene names: 126209321

Gene position: 2090944-2090045 (Counterclockwise)

Preceding gene: 126209328

Following gene: 126209320

Centisome position: 91.93

GC content: 44.11

Gene sequence:

>900_bases
ATGCAAAATTTTAATTACTTTACTCCGACCAAAATCGTATTCGGTAAAAACACCGAACAACAAGTCGGCGAATTAATTCA
AGCGCAACATTGCAAAAAAGTGTTGATTCATTACGGTAGCCAAAGTGCGAAAAAATCCGGTTTATTGGATCGTATTAAAG
CGTCATTAGATAACGCCGGTATCGCTTATATTGAATTGGGTGGTGTCGTACCGAATCCGTTATTGTCTTTGGTATATCAA
GTTATTGAATTATGCAAGCAAGAAGGCGTTGATTTTATTCTCGCCGTAGGCGGCGGTAGTGTTATCGACTCGGCAAAAGC
GATTGCTTACGGCGTAGCGGAAGAAGGCGATGTTTGGGAGCTGTATGACCGTAAACGTCAAGCAACCGCTTGTTTGCCGG
TTGCAACCGTTTTAACCATTTCAGCCGCCGGTAGTGAAATGAGTGACGGTTCGGTGATTACTAAAGACGAGGGCGGTATC
AAGCGCGGTTATAGTAATAACTTATCTCGCCCGAAATTCTCAATTTTAAATCCCGAATTAACCGTCACCTTACCGGCATA
TCAAACCGCAAGCGGTAACGTAGATATTTTAATGCATACGATGGAACGTTATTTCACACCGAACGGCACAATGGATATTA
CCGATACGATTGCGGAAAGTATTTTGAAAACCGTGATGAAAAATGCGCAAATCTTAGTAAAAGATCCGCAAAATTACCAA
GCCAGAGCGGAAATTATGTGGTGCGGTAGCCTGTCGCACAACGGTTTAACCGGCTGCGGCGGCGGTAACGGCGATTGGGC
AACGCATATGTTAGAACACGAGTTAAGCGGTATGTTTGATGTAACCCACGGTGCCGGTTTAGCTGCGATGTGGGGACATT
GGGCAAGATATGTTTACTAA

Upstream 100 bases:

>100_bases
TTCCTTAAACAGGAATTCTTTATTCGTAATTTATTCATTGTATAGGAATAAATCACTTTTTAGAATGCTTAAATAAATAG
TGATATGAGGAGAAACCAAT

Downstream 100 bases:

>100_bases
GATATTTTACCTCGCTTTGAACGTTTTGCGACTCAAGTAATGGGTGTTACACCAGAAACTACCGCAGAACAAACTGCTCT
AAAAGGTATTACAGCAATGG

Product: NADH-dependent butanol dehydrogenase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MQNFNYFTPTKIVFGKNTEQQVGELIQAQHCKKVLIHYGSQSAKKSGLLDRIKASLDNAGIAYIELGGVVPNPLLSLVYQ
VIELCKQEGVDFILAVGGGSVIDSAKAIAYGVAEEGDVWELYDRKRQATACLPVATVLTISAAGSEMSDGSVITKDEGGI
KRGYSNNLSRPKFSILNPELTVTLPAYQTASGNVDILMHTMERYFTPNGTMDITDTIAESILKTVMKNAQILVKDPQNYQ
ARAEIMWCGSLSHNGLTGCGGGNGDWATHMLEHELSGMFDVTHGAGLAAMWGHWARYVY

Sequences:

>Translated_299_residues
MQNFNYFTPTKIVFGKNTEQQVGELIQAQHCKKVLIHYGSQSAKKSGLLDRIKASLDNAGIAYIELGGVVPNPLLSLVYQ
VIELCKQEGVDFILAVGGGSVIDSAKAIAYGVAEEGDVWELYDRKRQATACLPVATVLTISAAGSEMSDGSVITKDEGGI
KRGYSNNLSRPKFSILNPELTVTLPAYQTASGNVDILMHTMERYFTPNGTMDITDTIAESILKTVMKNAQILVKDPQNYQ
ARAEIMWCGSLSHNGLTGCGGGNGDWATHMLEHELSGMFDVTHGAGLAAMWGHWARYVY
>Mature_299_residues
MQNFNYFTPTKIVFGKNTEQQVGELIQAQHCKKVLIHYGSQSAKKSGLLDRIKASLDNAGIAYIELGGVVPNPLLSLVYQ
VIELCKQEGVDFILAVGGGSVIDSAKAIAYGVAEEGDVWELYDRKRQATACLPVATVLTISAAGSEMSDGSVITKDEGGI
KRGYSNNLSRPKFSILNPELTVTLPAYQTASGNVDILMHTMERYFTPNGTMDITDTIAESILKTVMKNAQILVKDPQNYQ
ARAEIMWCGSLSHNGLTGCGGGNGDWATHMLEHELSGMFDVTHGAGLAAMWGHWARYVY

Specific function: Unknown

COG id: COG1979

COG function: function code C; Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1789386, Length=298, Percent_Identity=39.5973154362416, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI48994951, Length=294, Percent_Identity=25.8503401360544, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789163, Length=289, Percent_Identity=24.2214532871972, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87082107, Length=285, Percent_Identity=23.859649122807, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17537053, Length=292, Percent_Identity=23.2876712328767, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321181, Length=289, Percent_Identity=24.5674740484429, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 32456; Mature: 32456

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFNYFTPTKIVFGKNTEQQVGELIQAQHCKKVLIHYGSQSAKKSGLLDRIKASLDNAG
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
IAYIELGGVVPNPLLSLVYQVIELCKQEGVDFILAVGGGSVIDSAKAIAYGVAEEGDVWE
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHEECCCCCCCCHH
LYDRKRQATACLPVATVLTISAAGSEMSDGSVITKDEGGIKRGYSNNLSRPKFSILNPEL
HHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCE
TVTLPAYQTASGNVDILMHTMERYFTPNGTMDITDTIAESILKTVMKNAQILVKDPQNYQ
EEEECCEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
ARAEIMWCGSLSHNGLTGCGGGNGDWATHMLEHELSGMFDVTHGAGLAAMWGHWARYVY
EEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQNFNYFTPTKIVFGKNTEQQVGELIQAQHCKKVLIHYGSQSAKKSGLLDRIKASLDNAG
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
IAYIELGGVVPNPLLSLVYQVIELCKQEGVDFILAVGGGSVIDSAKAIAYGVAEEGDVWE
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHEECCCCCCCCHH
LYDRKRQATACLPVATVLTISAAGSEMSDGSVITKDEGGIKRGYSNNLSRPKFSILNPEL
HHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCE
TVTLPAYQTASGNVDILMHTMERYFTPNGTMDITDTIAESILKTVMKNAQILVKDPQNYQ
EEEECCEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
ARAEIMWCGSLSHNGLTGCGGGNGDWATHMLEHELSGMFDVTHGAGLAAMWGHWARYVY
EEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]