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The map label for this gene is holB [H]

Identifier: 126209278

GI number: 126209278

Start: 2038713

End: 2039696

Strand: Reverse

Name: holB [H]

Synonym: APL_1816

Alternate gene names: 126209278

Gene position: 2039696-2038713 (Counterclockwise)

Preceding gene: 126209279

Following gene: 126209277

Centisome position: 89.68

GC content: 43.09

Gene sequence:

>984_bases
ATGCTATATCCTTGGCTTCAACACACTTATCAACAACTTACAGAATCCTTTTTGCATGGGCGTGGTCATCACGCCCTGTT
ATTTAAAACCGATTCGGGTTTAGGGACGGAAACTCTTATTCGTCATTTTGCGCATTGGCTACTTTGCCAAAATCCGCACA
GGGAACAAGCTTGCCAACAGTGTAAAAGTTGTTTATTGACTGCTGCCGGAAACCACCCTGATTTCCATATTTTAGAGCCG
ATTGACGGTAAAGATATCGGTATTGATCAAGTGCGTGAAGCCAATGCCAAATTGCAAAACTTCTCGCAACAAGGTGGCAA
TGCGGTGGTATATATTCGTGGTGCGGAACGTTTAACCGAAGCAAGCAGCAATGCACTCTTAAAAACGCTTGAAGAACCAC
ATAACAGCGTTTATTTTCTGCTTGAAGCACCACTACAAGGTGCAATGCTCCCGACCATTCAAAGTCGCTGCCAAACGTGG
TTAATTCATCCGCCGGAAATGGATATTGCGTTAGATTGGTTGCAAGCAAACTGTAGCGGTGTTGATTTTTCCTCTTGTGA
AATTGCGATGCGTTTATGTCATCAACGCCCGCTGATTTGCAAAACTTTTTTAGAAAACGACCGCTTGCAAGCCCGCAAAA
CCTTTTTACAAACTTTTTGGCGTTTCTTTAAAAGCCGAGATGTATTGCTCCTCTTCACCGCATTTGATAAAGAAAAGGAA
CTGGTATTGCAGCAATTAGAATGGTTGGATAGCTTTTTTGCCGATGCGTTAAAAGCCAAAATGGATATTGCAATCGGTTG
GACAAATCCGGACTTACAAGCCGGTATTCTGCCGTTTAGCCAACAACTCTCCGTCCAAGCGCTGCTAAAAGGTCACCAAA
TTCTGCAACAAACCCGCCAAGACTTACAGCAAGTAAATGCGATGAATTTGGAATTGATGTTACTTAACTACCTGACAAAG
CTAGCGCTAGAGACGTTTGAATAA

Upstream 100 bases:

>100_bases
ACGAGAAAGCGGTAATTATTAATGCGGAACAATCTATCGAACAAGTTGCCGCTGACATTCAACAAGCGGTCGAAAACTTC
TTAAAAATTGCAAAATAAAT

Downstream 100 bases:

>100_bases
ATAAAATAGCATGTCTACCCACAACCTTGTAATTTTTACGTAGCCATACTGTAGAGAATACACGAGACTTCACTCTCCAA
TAAACATCATTTAACTGTCA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLYPWLQHTYQQLTESFLHGRGHHALLFKTDSGLGTETLIRHFAHWLLCQNPHREQACQQCKSCLLTAAGNHPDFHILEP
IDGKDIGIDQVREANAKLQNFSQQGGNAVVYIRGAERLTEASSNALLKTLEEPHNSVYFLLEAPLQGAMLPTIQSRCQTW
LIHPPEMDIALDWLQANCSGVDFSSCEIAMRLCHQRPLICKTFLENDRLQARKTFLQTFWRFFKSRDVLLLFTAFDKEKE
LVLQQLEWLDSFFADALKAKMDIAIGWTNPDLQAGILPFSQQLSVQALLKGHQILQQTRQDLQQVNAMNLELMLLNYLTK
LALETFE

Sequences:

>Translated_327_residues
MLYPWLQHTYQQLTESFLHGRGHHALLFKTDSGLGTETLIRHFAHWLLCQNPHREQACQQCKSCLLTAAGNHPDFHILEP
IDGKDIGIDQVREANAKLQNFSQQGGNAVVYIRGAERLTEASSNALLKTLEEPHNSVYFLLEAPLQGAMLPTIQSRCQTW
LIHPPEMDIALDWLQANCSGVDFSSCEIAMRLCHQRPLICKTFLENDRLQARKTFLQTFWRFFKSRDVLLLFTAFDKEKE
LVLQQLEWLDSFFADALKAKMDIAIGWTNPDLQAGILPFSQQLSVQALLKGHQILQQTRQDLQQVNAMNLELMLLNYLTK
LALETFE
>Mature_327_residues
MLYPWLQHTYQQLTESFLHGRGHHALLFKTDSGLGTETLIRHFAHWLLCQNPHREQACQQCKSCLLTAAGNHPDFHILEP
IDGKDIGIDQVREANAKLQNFSQQGGNAVVYIRGAERLTEASSNALLKTLEEPHNSVYFLLEAPLQGAMLPTIQSRCQTW
LIHPPEMDIALDWLQANCSGVDFSSCEIAMRLCHQRPLICKTFLENDRLQARKTFLQTFWRFFKSRDVLLLFTAFDKEKE
LVLQQLEWLDSFFADALKAKMDIAIGWTNPDLQAGILPFSQQLSVQALLKGHQILQQTRQDLQQVNAMNLELMLLNYLTK
LALETFE

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=320, Percent_Identity=34.0625, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786676, Length=151, Percent_Identity=31.1258278145695, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37489; Mature: 37489

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPWLQHTYQQLTESFLHGRGHHALLFKTDSGLGTETLIRHFAHWLLCQNPHREQACQQ
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
CKSCLLTAAGNHPDFHILEPIDGKDIGIDQVREANAKLQNFSQQGGNAVVYIRGAERLTE
HHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHH
ASSNALLKTLEEPHNSVYFLLEAPLQGAMLPTIQSRCQTWLIHPPEMDIALDWLQANCSG
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCEEECCCCCCCHHHHHHHCCCCC
VDFSSCEIAMRLCHQRPLICKTFLENDRLQARKTFLQTFWRFFKSRDVLLLFTAFDKEKE
CCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHH
LVLQQLEWLDSFFADALKAKMDIAIGWTNPDLQAGILPFSQQLSVQALLKGHQILQQTRQ
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DLQQVNAMNLELMLLNYLTKLALETFE
HHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLYPWLQHTYQQLTESFLHGRGHHALLFKTDSGLGTETLIRHFAHWLLCQNPHREQACQQ
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
CKSCLLTAAGNHPDFHILEPIDGKDIGIDQVREANAKLQNFSQQGGNAVVYIRGAERLTE
HHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHH
ASSNALLKTLEEPHNSVYFLLEAPLQGAMLPTIQSRCQTWLIHPPEMDIALDWLQANCSG
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCEEECCCCCCCHHHHHHHCCCCC
VDFSSCEIAMRLCHQRPLICKTFLENDRLQARKTFLQTFWRFFKSRDVLLLFTAFDKEKE
CCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHH
LVLQQLEWLDSFFADALKAKMDIAIGWTNPDLQAGILPFSQQLSVQALLKGHQILQQTRQ
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DLQQVNAMNLELMLLNYLTKLALETFE
HHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]