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The map label for this gene is menD

Identifier: 126209214

GI number: 126209214

Start: 1986901

End: 1988607

Strand: Reverse

Name: menD

Synonym: APL_1750

Alternate gene names: 126209214

Gene position: 1988607-1986901 (Counterclockwise)

Preceding gene: 126209221

Following gene: 126209213

Centisome position: 87.43

GC content: 46.22

Gene sequence:

>1707_bases
ATGACAGTCAGCACCTTTAACCGTACGTGGGCAAAAGTGATAGTGAACGCCTTATTACGTTACGGCGTAAAACATTTCTG
TATTGCGCCGGGTTCACGTTCAACACCTTTAACTCTCGAAGCCCTACAATTACAACAAAATCAACAAGCACAATGCCACA
GTCATTTTGATGAACGCGGATTGGGCTTTTTTGCTTTGGGTATCGCTAAAGTGACCAATGATCCGGTCGCAATTATTGTG
ACTTCCGGAACGGCGGTCGCTAATCTTTATCCGGCGATTATCGAAGCGAGCTTGACTCATCATAAATTGATCGTACTTTC
CGCCGATCGTCCGCCGGAATTAATCGGTTGCGGAGCAAATCAAGCGATTCCGCAGCAAGGTATTTTCGCCGATTATCCGA
TAGCCGGCGTTAATTTGCCGAAACCTGCGGAGCACTATAACGCCGGCTGGTTAGTTGCAACGATTGAACAGGCTTGCGTT
ACACAAAGCCAACAAGGCGGTGTGGTACATATCAATGCGCCGTTTGCCGAGCCGCTTTATGAAGCGGATGAAAACGCAAT
CAACACACATCCGTGGTTAAAGCCGATTCAAAGCTGGTTAATCAATCCGCAAACCAAATGGATTAACAGTCAAACGATTC
AAAGCGAAGTGTCAATGCACGAGAATTGGGATTATTGGCGTACCAAACGCGGTGTGATTGTGGTCGGTAAATTGCCGGTC
GAACAAGGTATCGGTATTAAAGCTTGGGCGGAAACGCTCGGTTGGTGTTTGATTACCGACGTACAATCTTGTGTAGATGC
CAATCTGCCGTATGCGGATATTTGGCTTTCCAACAACACGGTTCACCAACGCCTGTTACAAGCGGATATTGTGATTCAAT
TCGGCGGTCAAATCGTGAGTAAAAGGGTAAATAAATTCCTTGAAGCGTTTAAAGGCGAATTTTGGCAAGTGGATGAATAC
AGCGACTATCTTAACCCGTTTGCACATCACCAAACCCGTTTTGTGGCAAAAGCGCATCATTTCTTACGCGTACATCCGCC
GCTTCGCCAAAAACCGTGGTTATTGGAACCGCTCGCACTTTCACAATTCTGCGCCGGTTTTATCGAACAACAAGTCGGCG
GTAGCCTGAATGAGGCTTCATTAGCCCATCATATTGAAGAAGTGTTGGCAACCAGCGGTAATTTATTTATCGGTAACAGC
TTATTCGTACGTTTAGTTGATGCCCTATGTAAATTACCGGAAGGTTATCCGGTTTATACCAACCGCGGTGCAAGCGGCAT
TGACGGTTTAATCGCAACCATGGCGGGGGTGGCAAAAGGCAGCGGTCAGCCGACCGTCGGCGTTATCGGCGACATTTCCG
CATTACATGATCTGAATTCGGTGTCGCTACTTAACAAAATCAGCCATCCGTGCATTTTATTTGTGATCAACAATAGCGGC
GGAGCGATTTTCGATATGTTACCGGTGGAAGCGCAAGCGAAAGAACAATTCTATCGCTTATCGCATAACTATGAATTCGC
ACCGATTGCGACCATGTTCGGCATTGAATATATCCGTCCGTTTACTTGGGCCGATCTGAAAGCGAAACTGAAACTCGCTT
ACGGACGTAAAGGCGTGACTATCGTTGAAATTAAAGTGAACGATCAAGACGGTAGCAATCTTTACAAATCCTTAGTTAAA
CAAATCTCACAAGCGGAAATTGCCTAA

Upstream 100 bases:

>100_bases
GAATAACAAGCGGTAAATTTCCACCCATTTTTGCCAGATTTTGCTACAATCTCGCCATTTAGTGAAAATTAAACCAGTTA
TAGAGAAACAGGGAAGAGAT

Downstream 100 bases:

>100_bases
TGCTCCACGCAACATGGCATCGTGAAACGGGTATGCCGGTGGTTTTTCTGCACGGTTTACTCGGTTCGCAACAAGATTGG
CAAGCGGTGCTAGACCGCTT

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MTVSTFNRTWAKVIVNALLRYGVKHFCIAPGSRSTPLTLEALQLQQNQQAQCHSHFDERGLGFFALGIAKVTNDPVAIIV
TSGTAVANLYPAIIEASLTHHKLIVLSADRPPELIGCGANQAIPQQGIFADYPIAGVNLPKPAEHYNAGWLVATIEQACV
TQSQQGGVVHINAPFAEPLYEADENAINTHPWLKPIQSWLINPQTKWINSQTIQSEVSMHENWDYWRTKRGVIVVGKLPV
EQGIGIKAWAETLGWCLITDVQSCVDANLPYADIWLSNNTVHQRLLQADIVIQFGGQIVSKRVNKFLEAFKGEFWQVDEY
SDYLNPFAHHQTRFVAKAHHFLRVHPPLRQKPWLLEPLALSQFCAGFIEQQVGGSLNEASLAHHIEEVLATSGNLFIGNS
LFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGVAKGSGQPTVGVIGDISALHDLNSVSLLNKISHPCILFVINNSG
GAIFDMLPVEAQAKEQFYRLSHNYEFAPIATMFGIEYIRPFTWADLKAKLKLAYGRKGVTIVEIKVNDQDGSNLYKSLVK
QISQAEIA

Sequences:

>Translated_568_residues
MTVSTFNRTWAKVIVNALLRYGVKHFCIAPGSRSTPLTLEALQLQQNQQAQCHSHFDERGLGFFALGIAKVTNDPVAIIV
TSGTAVANLYPAIIEASLTHHKLIVLSADRPPELIGCGANQAIPQQGIFADYPIAGVNLPKPAEHYNAGWLVATIEQACV
TQSQQGGVVHINAPFAEPLYEADENAINTHPWLKPIQSWLINPQTKWINSQTIQSEVSMHENWDYWRTKRGVIVVGKLPV
EQGIGIKAWAETLGWCLITDVQSCVDANLPYADIWLSNNTVHQRLLQADIVIQFGGQIVSKRVNKFLEAFKGEFWQVDEY
SDYLNPFAHHQTRFVAKAHHFLRVHPPLRQKPWLLEPLALSQFCAGFIEQQVGGSLNEASLAHHIEEVLATSGNLFIGNS
LFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGVAKGSGQPTVGVIGDISALHDLNSVSLLNKISHPCILFVINNSG
GAIFDMLPVEAQAKEQFYRLSHNYEFAPIATMFGIEYIRPFTWADLKAKLKLAYGRKGVTIVEIKVNDQDGSNLYKSLVK
QISQAEIA
>Mature_567_residues
TVSTFNRTWAKVIVNALLRYGVKHFCIAPGSRSTPLTLEALQLQQNQQAQCHSHFDERGLGFFALGIAKVTNDPVAIIVT
SGTAVANLYPAIIEASLTHHKLIVLSADRPPELIGCGANQAIPQQGIFADYPIAGVNLPKPAEHYNAGWLVATIEQACVT
QSQQGGVVHINAPFAEPLYEADENAINTHPWLKPIQSWLINPQTKWINSQTIQSEVSMHENWDYWRTKRGVIVVGKLPVE
QGIGIKAWAETLGWCLITDVQSCVDANLPYADIWLSNNTVHQRLLQADIVIQFGGQIVSKRVNKFLEAFKGEFWQVDEYS
DYLNPFAHHQTRFVAKAHHFLRVHPPLRQKPWLLEPLALSQFCAGFIEQQVGGSLNEASLAHHIEEVLATSGNLFIGNSL
FVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGVAKGSGQPTVGVIGDISALHDLNSVSLLNKISHPCILFVINNSGG
AIFDMLPVEAQAKEQFYRLSHNYEFAPIATMFGIEYIRPFTWADLKAKLKLAYGRKGVTIVEIKVNDQDGSNLYKSLVKQ
ISQAEIA

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=566, Percent_Identity=47.3498233215548, Blast_Score=520, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_ACTP2 (A3N348)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054439.1
- ProteinModelPortal:   A3N348
- STRING:   A3N348
- GeneID:   4849122
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1750
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- PhylomeDB:   A3N348
- ProtClustDB:   PRK07449
- BioCyc:   APLE416269:APL_1750-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 62667; Mature: 62536

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSTFNRTWAKVIVNALLRYGVKHFCIAPGSRSTPLTLEALQLQQNQQAQCHSHFDERG
CCCCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCEEHHHHHHHCCCHHHHHHHHHHCC
LGFFALGIAKVTNDPVAIIVTSGTAVANLYPAIIEASLTHHKLIVLSADRPPELIGCGAN
CCEEEEEHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCC
QAIPQQGIFADYPIAGVNLPKPAEHYNAGWLVATIEQACVTQSQQGGVVHINAPFAEPLY
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEEHHHHHHCCCCCCCEEEEECCCCCHHH
EADENAINTHPWLKPIQSWLINPQTKWINSQTIQSEVSMHENWDYWRTKRGVIVVGKLPV
HCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCCHHCCCCCEEEEECCCH
EQGIGIKAWAETLGWCLITDVQSCVDANLPYADIWLSNNTVHQRLLQADIVIQFGGQIVS
HHCCCHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEECCHHHHH
KRVNKFLEAFKGEFWQVDEYSDYLNPFAHHQTRFVAKAHHFLRVHPPLRQKPWLLEPLAL
HHHHHHHHHHCCCCEEECCHHHHHCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHH
SQFCAGFIEQQVGGSLNEASLAHHIEEVLATSGNLFIGNSLFVRLVDALCKLPEGYPVYT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCCEEE
NRGASGIDGLIATMAGVAKGSGQPTVGVIGDISALHDLNSVSLLNKISHPCILFVINNSG
CCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHCCHHHHHHHHCCCCEEEEEEECCC
GAIFDMLPVEAQAKEQFYRLSHNYEFAPIATMFGIEYIRPFTWADLKAKLKLAYGRKGVT
CEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHEEEEEEECCCCCE
IVEIKVNDQDGSNLYKSLVKQISQAEIA
EEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVSTFNRTWAKVIVNALLRYGVKHFCIAPGSRSTPLTLEALQLQQNQQAQCHSHFDERG
CCCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCEEHHHHHHHCCCHHHHHHHHHHCC
LGFFALGIAKVTNDPVAIIVTSGTAVANLYPAIIEASLTHHKLIVLSADRPPELIGCGAN
CCEEEEEHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCC
QAIPQQGIFADYPIAGVNLPKPAEHYNAGWLVATIEQACVTQSQQGGVVHINAPFAEPLY
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEEHHHHHHCCCCCCCEEEEECCCCCHHH
EADENAINTHPWLKPIQSWLINPQTKWINSQTIQSEVSMHENWDYWRTKRGVIVVGKLPV
HCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCCHHCCCCCEEEEECCCH
EQGIGIKAWAETLGWCLITDVQSCVDANLPYADIWLSNNTVHQRLLQADIVIQFGGQIVS
HHCCCHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEECCHHHHH
KRVNKFLEAFKGEFWQVDEYSDYLNPFAHHQTRFVAKAHHFLRVHPPLRQKPWLLEPLAL
HHHHHHHHHHCCCCEEECCHHHHHCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHH
SQFCAGFIEQQVGGSLNEASLAHHIEEVLATSGNLFIGNSLFVRLVDALCKLPEGYPVYT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCCEEE
NRGASGIDGLIATMAGVAKGSGQPTVGVIGDISALHDLNSVSLLNKISHPCILFVINNSG
CCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHCCHHHHHHHHCCCCEEEEEEECCC
GAIFDMLPVEAQAKEQFYRLSHNYEFAPIATMFGIEYIRPFTWADLKAKLKLAYGRKGVT
CEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHEEEEEEECCCCCE
IVEIKVNDQDGSNLYKSLVKQISQAEIA
EEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA