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The map label for this gene is hslU

Identifier: 126209199

GI number: 126209199

Start: 1970984

End: 1972306

Strand: Reverse

Name: hslU

Synonym: APL_1735

Alternate gene names: 126209199

Gene position: 1972306-1970984 (Counterclockwise)

Preceding gene: 126209200

Following gene: 126209198

Centisome position: 86.71

GC content: 43.16

Gene sequence:

>1323_bases
ATGTCAATGACCCCTCGTGAAATCGTGTCGGAATTGGACGCGCACATTATCGGACAAAACGAAGCAAAACGTGCGGTGGC
AATTGCGCTACGCAACCGTTGGAGAAGGATGCAATTACCGGAAGATCTCCGCCAAGAAGTTACTCCGAAAAACATTCTGA
TGATCGGCCCGACCGGTGTAGGTAAAACCGAAATTGCACGCCGTTTAGCAAAATTAGCCAACGCACCTTTTATCAAAGTG
GAAGCAACAAAATTCACCGAAGTGGGCTATGTAGGTAAAGAAGTCGATTCAATTATCAAAGATCTGACCGATGTGGCTGT
GAAATTAGTTAAATCACAAGCGGTCGAAAAAAACCGTATGCGTGCGCAAGATGCGGCGGAAGATCGCATTTTAGATGTGT
TATTACCACCGGCAAAAGATCAATGGGGTAACGTACAAGAAAGCGATAACAGCTCAACCCGCCAAGTGTTCCGCAAAAAA
TTGCGTGAAGGTCAGCTTGATGACAAAGAAATCGAAATCGATGTAGCGGCACAAGTGAGCGTAGAAATTATGACGCCTCC
GGGTATGGAAGAAATGACTTCTCAGCTTCAATCGTTATTTGAAGGTATGTCACCGAATAAAACCAAAAAACGCAAAATGA
AAATCAAAGATGCGTTAAAAGTGATGGTGGACGAAGAAGCGGCAAAACTGGTGAATCCGGAAGAGTTGAAACAACAAGCG
ATTGAAGCGGTTGAACAACACGGTATCGTATTTATTGATGAGATCGATAAAATCTGTAAAAAAGGCGAACACTCGGGCGG
CGATGTTTCTCGTGAAGGTGTACAACGTGATTTACTGCCGATTATTGAAGGTTCTACGGTCAATACCAAACACGGTATGG
TAAAAACCGACCATATTTTATTTATCTGTTCAGGTGCATTCCAAGTGGCTCGCCCTTCAGATTTATTACCGGAATTACAA
GGTCGTTTACCGATTCGCGTCGAATTAAAATCACTGACTAAAGAAGATTTCGAACGTATCCTCACCGAACCGAACGCTTC
ATTAACGCTACAATATCGTGAGCTAATGAAAACCGAAGGTGTAGAAATCGAATTTACCAAAGACGGTATCAGTAAAATCG
CCGAATCGGCATTCCGTGTAAACGAAAAAACCGAAAACATCGGTGCTCGTCGCTTACACACCGTATTAGAACGCCTAATG
GACGGAATTTCATTCGACGCAAGCGAACGCTCAGGCGAGAAAATCGTCATCGATGAGAAATACGTACAAGCTGCGTTAAA
TGACGTAGTAGAGAATGAAGATTTAAGTCGTTTTATTCTTTAA

Upstream 100 bases:

>100_bases
TATTGATATTTACTCAAATCATAATCACGTAATCGAAGAAGTTTAAAAAATGTCCCCCTCTTTGCTAAAGAGGGGGAGTT
ATTTAGAAGGAACAAAAAAT

Downstream 100 bases:

>100_bases
TTAACGGATAACAAGCGGTCAAAATTTGCGGATTTTTTGCAGATTTTGACCACTTTTAATATACGAGAGAAAATGAAATA
CTTTTATATTTTATTAGTTC

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSMTPREIVSELDAHIIGQNEAKRAVAIALRNRWRRMQLPEDLRQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVDSIIKDLTDVAVKLVKSQAVEKNRMRAQDAAEDRILDVLLPPAKDQWGNVQESDNSSTRQVFRKK
LREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPNKTKKRKMKIKDALKVMVDEEAAKLVNPEELKQQA
IEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQRDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQ
GRLPIRVELKSLTKEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISKIAESAFRVNEKTENIGARRLHTVLERLM
DGISFDASERSGEKIVIDEKYVQAALNDVVENEDLSRFIL

Sequences:

>Translated_440_residues
MSMTPREIVSELDAHIIGQNEAKRAVAIALRNRWRRMQLPEDLRQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVDSIIKDLTDVAVKLVKSQAVEKNRMRAQDAAEDRILDVLLPPAKDQWGNVQESDNSSTRQVFRKK
LREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPNKTKKRKMKIKDALKVMVDEEAAKLVNPEELKQQA
IEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQRDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQ
GRLPIRVELKSLTKEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISKIAESAFRVNEKTENIGARRLHTVLERLM
DGISFDASERSGEKIVIDEKYVQAALNDVVENEDLSRFIL
>Mature_439_residues
SMTPREIVSELDAHIIGQNEAKRAVAIALRNRWRRMQLPEDLRQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVE
ATKFTEVGYVGKEVDSIIKDLTDVAVKLVKSQAVEKNRMRAQDAAEDRILDVLLPPAKDQWGNVQESDNSSTRQVFRKKL
REGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPNKTKKRKMKIKDALKVMVDEEAAKLVNPEELKQQAI
EAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQRDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQG
RLPIRVELKSLTKEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISKIAESAFRVNEKTENIGARRLHTVLERLMD
GISFDASERSGEKIVIDEKYVQAALNDVVENEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=440, Percent_Identity=77.0454545454545, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=232, Percent_Identity=34.4827586206897, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=486, Percent_Identity=26.3374485596708, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_ACTP2 (A3N333)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054424.1
- ProteinModelPortal:   A3N333
- SMR:   A3N333
- STRING:   A3N333
- GeneID:   4849314
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1735
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- PhylomeDB:   A3N333
- ProtClustDB:   PRK05201
- BioCyc:   APLE416269:APL_1735-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 49477; Mature: 49345

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: BINDING 17-17 BINDING 253-253 BINDING 318-318 BINDING 390-390

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTPREIVSELDAHIIGQNEAKRAVAIALRNRWRRMQLPEDLRQEVTPKNILMIGPTGV
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCC
GKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIKDLTDVAVKLVKSQAVEKNRM
CHHHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAQDAAEDRILDVLLPPAKDQWGNVQESDNSSTRQVFRKKLREGQLDDKEIEIDVAAQVS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEE
VEIMTPPGMEEMTSQLQSLFEGMSPNKTKKRKMKIKDALKVMVDEEAAKLVNPEELKQQA
EEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQRDLLPIIEGSTVNTKHGMVKTDHIL
HHHHHHCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCEE
FICSGAFQVARPSDLLPELQGRLPIRVELKSLTKEDFERILTEPNASLTLQYRELMKTEG
EEECCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCC
VEIEFTKDGISKIAESAFRVNEKTENIGARRLHTVLERLMDGISFDASERSGEKIVIDEK
CEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHH
YVQAALNDVVENEDLSRFIL
HHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SMTPREIVSELDAHIIGQNEAKRAVAIALRNRWRRMQLPEDLRQEVTPKNILMIGPTGV
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCC
GKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIKDLTDVAVKLVKSQAVEKNRM
CHHHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAQDAAEDRILDVLLPPAKDQWGNVQESDNSSTRQVFRKKLREGQLDDKEIEIDVAAQVS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEE
VEIMTPPGMEEMTSQLQSLFEGMSPNKTKKRKMKIKDALKVMVDEEAAKLVNPEELKQQA
EEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQRDLLPIIEGSTVNTKHGMVKTDHIL
HHHHHHCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCEE
FICSGAFQVARPSDLLPELQGRLPIRVELKSLTKEDFERILTEPNASLTLQYRELMKTEG
EEECCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCC
VEIEFTKDGISKIAESAFRVNEKTENIGARRLHTVLERLMDGISFDASERSGEKIVIDEK
CEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHH
YVQAALNDVVENEDLSRFIL
HHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA