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The map label for this gene is rimO

Identifier: 126209100

GI number: 126209100

Start: 1861550

End: 1862881

Strand: Reverse

Name: rimO

Synonym: APL_1636

Alternate gene names: 126209100

Gene position: 1862881-1861550 (Counterclockwise)

Preceding gene: 126209101

Following gene: 126209099

Centisome position: 81.9

GC content: 44.14

Gene sequence:

>1332_bases
ATGAGTACTACACCTAATATTGGTTTTATTAGTCTTGGCTGTCCGAAGAATTTAGTCGATTCGGAACGTATTCTGACTGA
ATTACGTACAGACGGTTATAACATCATTCCAAGTTATGAAAATGCGGATTTAGTCATTGTAAATACCTGCGGCTTTATTG
ATAGTGCGGTGCAGGAGTCGTTAGAAGCGATCGGCGAAGCATTGGAAGAAAACGGTAAAGTGATTGTAACCGGCTGTTTA
GGGGCGAAAGAAAACCAAATTCGTGAAGTACATCCGAAAGTTTTGGAGATTACCGGCCCTCACAGTTATGAAGCGGTAAT
GAAGCACGTGCATAAATACGTACCTCGTCCGGAGCGTAACATTTATACCAGTCTTGTGCCGGCGCAAGGGGTTAAACTTA
CTCCTAAACATTACGCCTATTTAAAAATTTCGGAAGGTTGCGACCATCGTTGTACCTTCTGTATTATTCCGTCAATGCGC
GGCGATTTAGATAGCCGTCCGATTGTGCAAGTGTTAGATGAAGCAAAACGCCTTGCCGATTCGGGCGTAAAAGAGTTACT
CATCGTTTCTCAAGATACTTCCGCTTATGCGCTGGATCAGTCAAAAGAAAATCAAAATAAAACGGTTTTCTGGAACGGTG
CGCCGATTAAAAATAATCTTATCACTTTATGTGAGCAGTTAGGCTCGCTAGGTATTTGGGTTCGCCTACATTATGTCTAT
CCGTATCCGCACGTGGACGACTTAATTCCGCTTATGGCGCAAGGTAAAATTCTGCCGTATTTGGATATTCCGTTACAGCA
CGCCAGCCCGAAAGTGCTGAAAGCGATGAAACGCCCCGGCGCTATAGATCGTACTTTGGAACGTATAAAAAAATGGCGTG
AGATTTGTCCGAAATTAACCTTACGTTCAACCTTTATTGTCGGCTTCCCGGGAGAAACGGAAGAAGATTTCCAAATGTTA
TTGGATTTCTTGGAAGAAGCCCAGTTAGATCGCGTCGGCTGCTTCAAATTTAGCCCAGTAGAAGGGGCGGTTGCAACCGA
AATGGCGGATCAAGTGCCGGAAGACGTGAAAGAAGAACGCTTCCATCGCTTTATGCAAGTTCAACAACGTATTTCCGCTG
CCCGTTTACAACAAAAAGTGGGTAAAACGCTTGCAGTCATTATTGATGAAATTGACGAAGAAGGTATTATCGGTCGTTCA
ATGGCGGATGCGCCGGAAATTGACGGCGTAGTCTATGTCGATAACCTTTCCGAGCAAGAAGTGAAAGTCGGTCAAGTGAT
TTCGGTAAGCATTACCAACGCAGACGAATATGATCTCTGGGGAACTTGTTAA

Upstream 100 bases:

>100_bases
CTATTTGATAGCAACTTCTGTACAATATTCACACTTTGCCTACTGATTCTCAGTAGGTCTTTTTTTTATTTGATTACCTA
ATGATATTTTTGAGAAATTT

Downstream 100 bases:

>100_bases
TTGTAGGGATGATATATGGAAAACGAAAATAAACCGAATGTAGCTAATTTTGAAGCGGCGGTTGCGGCTAAAGATTATGA
AAAAGCTTGCTCCGAATTAC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSTTPNIGFISLGCPKNLVDSERILTELRTDGYNIIPSYENADLVIVNTCGFIDSAVQESLEAIGEALEENGKVIVTGCL
GAKENQIREVHPKVLEITGPHSYEAVMKHVHKYVPRPERNIYTSLVPAQGVKLTPKHYAYLKISEGCDHRCTFCIIPSMR
GDLDSRPIVQVLDEAKRLADSGVKELLIVSQDTSAYALDQSKENQNKTVFWNGAPIKNNLITLCEQLGSLGIWVRLHYVY
PYPHVDDLIPLMAQGKILPYLDIPLQHASPKVLKAMKRPGAIDRTLERIKKWREICPKLTLRSTFIVGFPGETEEDFQML
LDFLEEAQLDRVGCFKFSPVEGAVATEMADQVPEDVKEERFHRFMQVQQRISAARLQQKVGKTLAVIIDEIDEEGIIGRS
MADAPEIDGVVYVDNLSEQEVKVGQVISVSITNADEYDLWGTC

Sequences:

>Translated_443_residues
MSTTPNIGFISLGCPKNLVDSERILTELRTDGYNIIPSYENADLVIVNTCGFIDSAVQESLEAIGEALEENGKVIVTGCL
GAKENQIREVHPKVLEITGPHSYEAVMKHVHKYVPRPERNIYTSLVPAQGVKLTPKHYAYLKISEGCDHRCTFCIIPSMR
GDLDSRPIVQVLDEAKRLADSGVKELLIVSQDTSAYALDQSKENQNKTVFWNGAPIKNNLITLCEQLGSLGIWVRLHYVY
PYPHVDDLIPLMAQGKILPYLDIPLQHASPKVLKAMKRPGAIDRTLERIKKWREICPKLTLRSTFIVGFPGETEEDFQML
LDFLEEAQLDRVGCFKFSPVEGAVATEMADQVPEDVKEERFHRFMQVQQRISAARLQQKVGKTLAVIIDEIDEEGIIGRS
MADAPEIDGVVYVDNLSEQEVKVGQVISVSITNADEYDLWGTC
>Mature_442_residues
STTPNIGFISLGCPKNLVDSERILTELRTDGYNIIPSYENADLVIVNTCGFIDSAVQESLEAIGEALEENGKVIVTGCLG
AKENQIREVHPKVLEITGPHSYEAVMKHVHKYVPRPERNIYTSLVPAQGVKLTPKHYAYLKISEGCDHRCTFCIIPSMRG
DLDSRPIVQVLDEAKRLADSGVKELLIVSQDTSAYALDQSKENQNKTVFWNGAPIKNNLITLCEQLGSLGIWVRLHYVYP
YPHVDDLIPLMAQGKILPYLDIPLQHASPKVLKAMKRPGAIDRTLERIKKWREICPKLTLRSTFIVGFPGETEEDFQMLL
DFLEEAQLDRVGCFKFSPVEGAVATEMADQVPEDVKEERFHRFMQVQQRISAARLQQKVGKTLAVIIDEIDEEGIIGRSM
ADAPEIDGVVYVDNLSEQEVKVGQVISVSITNADEYDLWGTC

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=409, Percent_Identity=28.361858190709, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI28872784, Length=427, Percent_Identity=26.6978922716628, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=26.4150943396226, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1787057, Length=442, Percent_Identity=75.3393665158371, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=455, Percent_Identity=26.1538461538462, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=27.6008492569002, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71996771, Length=265, Percent_Identity=25.2830188679245, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356207, Length=483, Percent_Identity=26.7080745341615, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=24.4444444444444, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_ACTP2 (A3N2T4)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054325.1
- ProteinModelPortal:   A3N2T4
- SMR:   A3N2T4
- STRING:   A3N2T4
- GeneID:   4849937
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1636
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   A3N2T4
- ProtClustDB:   PRK14862
- BioCyc:   APLE416269:APL_1636-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49597; Mature: 49465

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPNIGFISLGCPKNLVDSERILTELRTDGYNIIPSYENADLVIVNTCGFIDSAVQES
CCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCHHHHHHHHH
LEAIGEALEENGKVIVTGCLGAKENQIREVHPKVLEITGPHSYEAVMKHVHKYVPRPERN
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
IYTSLVPAQGVKLTPKHYAYLKISEGCDHRCTFCIIPSMRGDLDSRPIVQVLDEAKRLAD
HHHHCCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
SGVKELLIVSQDTSAYALDQSKENQNKTVFWNGAPIKNNLITLCEQLGSLGIWVRLHYVY
CCCCEEEEEECCCCCEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEEE
PYPHVDDLIPLMAQGKILPYLDIPLQHASPKVLKAMKRPGAIDRTLERIKKWREICPKLT
CCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCE
LRSTFIVGFPGETEEDFQMLLDFLEEAQLDRVGCFKFSPVEGAVATEMADQVPEDVKEER
ECCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCHHHHHHHH
FHRFMQVQQRISAARLQQKVGKTLAVIIDEIDEEGIIGRSMADAPEIDGVVYVDNLSEQE
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
VKVGQVISVSITNADEYDLWGTC
EEECEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
STTPNIGFISLGCPKNLVDSERILTELRTDGYNIIPSYENADLVIVNTCGFIDSAVQES
CCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCHHHHHHHHH
LEAIGEALEENGKVIVTGCLGAKENQIREVHPKVLEITGPHSYEAVMKHVHKYVPRPERN
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
IYTSLVPAQGVKLTPKHYAYLKISEGCDHRCTFCIIPSMRGDLDSRPIVQVLDEAKRLAD
HHHHCCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
SGVKELLIVSQDTSAYALDQSKENQNKTVFWNGAPIKNNLITLCEQLGSLGIWVRLHYVY
CCCCEEEEEECCCCCEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEEE
PYPHVDDLIPLMAQGKILPYLDIPLQHASPKVLKAMKRPGAIDRTLERIKKWREICPKLT
CCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCE
LRSTFIVGFPGETEEDFQMLLDFLEEAQLDRVGCFKFSPVEGAVATEMADQVPEDVKEER
ECCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCHHHHHHHH
FHRFMQVQQRISAARLQQKVGKTLAVIIDEIDEEGIIGRSMADAPEIDGVVYVDNLSEQE
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
VKVGQVISVSITNADEYDLWGTC
EEECEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA