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The map label for this gene is apuR [H]

Identifier: 126209089

GI number: 126209089

Start: 1848084

End: 1848839

Strand: Direct

Name: apuR [H]

Synonym: APL_1625

Alternate gene names: 126209089

Gene position: 1848084-1848839 (Clockwise)

Preceding gene: 126209070

Following gene: 126209104

Centisome position: 81.25

GC content: 45.11

Gene sequence:

>756_bases
TTGTTTAAACGTACGACGCGTAACATTACCCTAACCAATTTCGGCGAAGAGTTTTTATTACAGGCGGAACAATTACTGAG
AGACAGCGAAAAATTGTTCGGCTTAGGCAAGAAAAAACAGGATGAAATGGCGGGCGTCGTGCGTATCACGCTACCGAAAT
TACGTGATCAGGAGGAAATTCTATTTGAGTTACTGCAAGCGCTGGAGCCCTATCCGGAATTAGTGATTGACTGGCGGGTT
GATGCCGCGCGTTTAGATAACGTTAAACATAGAATCGATATCGGTATCCGAGCCGGTCATGACCCCGATCCTAATTTTAT
TATTCGTCCTTTAACCAAAATGCACGAGATTTTCGTTGCCTCCCCCGCTTTTATTCAGCGTTGGGGTATGCCGAAAAATC
TGGACGATATGCAACGAAACTTCCCCATCAGCAGCTTATTAAACGCCAATACCGGACGGCCTTGGGAAATTTATCTTAAC
GAGCATCAAGTGCTTGTTCCGCAAAAAATCGGTTTTATTACGACGGACTTATACTGCGAGCTTCAGGCGGCATTAGCCGG
TAAAACCATTACGCATATCGGCAATACGGTGTGTAAGCCGTATCTCGAAAGCGGTCAGCTTATTCAACTTTTTCCCGAAC
AGCAGTCCGAATTATGGCAACTGTATTTATACCGCCCGTATCAAGCGGTAACATCGCCGAGAGTACTTAAAGTTTTTGAT
CTGCTGACCGAGATTATGCAACGCCGTTATAGCTAA

Upstream 100 bases:

>100_bases
TCAAACACTCAATTTTAGAGAAGCCGCCAATCAATTGGCGGTTTCTCCGCCGGTTATTAGCCGAGCCGTAAATGAGCTTG
AATCGGAGCTAGGCGAACAA

Downstream 100 bases:

>100_bases
CAAGAAAAAAGCGGTCAAATTTTGCAAAAAAATTGCAATCTCTGACCGCCAAGGTGAACACTGTTCTCTTTAAACGGATT
AAACGTTTGCCGGCTTAATC

Product: putative LysR-type transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MFKRTTRNITLTNFGEEFLLQAEQLLRDSEKLFGLGKKKQDEMAGVVRITLPKLRDQEEILFELLQALEPYPELVIDWRV
DAARLDNVKHRIDIGIRAGHDPDPNFIIRPLTKMHEIFVASPAFIQRWGMPKNLDDMQRNFPISSLLNANTGRPWEIYLN
EHQVLVPQKIGFITTDLYCELQAALAGKTITHIGNTVCKPYLESGQLIQLFPEQQSELWQLYLYRPYQAVTSPRVLKVFD
LLTEIMQRRYS

Sequences:

>Translated_251_residues
MFKRTTRNITLTNFGEEFLLQAEQLLRDSEKLFGLGKKKQDEMAGVVRITLPKLRDQEEILFELLQALEPYPELVIDWRV
DAARLDNVKHRIDIGIRAGHDPDPNFIIRPLTKMHEIFVASPAFIQRWGMPKNLDDMQRNFPISSLLNANTGRPWEIYLN
EHQVLVPQKIGFITTDLYCELQAALAGKTITHIGNTVCKPYLESGQLIQLFPEQQSELWQLYLYRPYQAVTSPRVLKVFD
LLTEIMQRRYS
>Mature_251_residues
MFKRTTRNITLTNFGEEFLLQAEQLLRDSEKLFGLGKKKQDEMAGVVRITLPKLRDQEEILFELLQALEPYPELVIDWRV
DAARLDNVKHRIDIGIRAGHDPDPNFIIRPLTKMHEIFVASPAFIQRWGMPKNLDDMQRNFPISSLLNANTGRPWEIYLN
EHQVLVPQKIGFITTDLYCELQAALAGKTITHIGNTVCKPYLESGQLIQLFPEQQSELWQLYLYRPYQAVTSPRVLKVFD
LLTEIMQRRYS

Specific function: Positive regulator required for L-tartrate-dependent anaerobic growth on glycerol. Induces expression of the ttdA-ttdB- ygjE operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789440, Length=230, Percent_Identity=25.6521739130435, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI145693193, Length=258, Percent_Identity=24.8062015503876, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081978, Length=256, Percent_Identity=26.171875, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=254, Percent_Identity=23.2283464566929, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=230, Percent_Identity=22.6086956521739, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 29226; Mature: 29226

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRTTRNITLTNFGEEFLLQAEQLLRDSEKLFGLGKKKQDEMAGVVRITLPKLRDQEEI
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCEEEEECCCCCCHHHH
LFELLQALEPYPELVIDWRVDAARLDNVKHRIDIGIRAGHDPDPNFIIRPLTKMHEIFVA
HHHHHHHHCCCCCEEEEECCCHHHHCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHC
SPAFIQRWGMPKNLDDMQRNFPISSLLNANTGRPWEIYLNEHQVLVPQKIGFITTDLYCE
CHHHHHHCCCCCCHHHHHHCCCHHHHHCCCCCCCEEEEECCCEEECCCCCCEEEHHHHHH
LQAALAGKTITHIGNTVCKPYLESGQLIQLFPEQQSELWQLYLYRPYQAVTSPRVLKVFD
HHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCHHHHCCCHHHHHHH
LLTEIMQRRYS
HHHHHHHHHCC
>Mature Secondary Structure
MFKRTTRNITLTNFGEEFLLQAEQLLRDSEKLFGLGKKKQDEMAGVVRITLPKLRDQEEI
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCEEEEECCCCCCHHHH
LFELLQALEPYPELVIDWRVDAARLDNVKHRIDIGIRAGHDPDPNFIIRPLTKMHEIFVA
HHHHHHHHCCCCCEEEEECCCHHHHCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHC
SPAFIQRWGMPKNLDDMQRNFPISSLLNANTGRPWEIYLNEHQVLVPQKIGFITTDLYCE
CHHHHHHCCCCCCHHHHHHCCCHHHHHCCCCCCCEEEEECCCEEECCCCCCEEEHHHHHH
LQAALAGKTITHIGNTVCKPYLESGQLIQLFPEQQSELWQLYLYRPYQAVTSPRVLKVFD
HHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCHHHHCCCHHHHHHH
LLTEIMQRRYS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]