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The map label for this gene is selB [H]

Identifier: 126209025

GI number: 126209025

Start: 1785447

End: 1787282

Strand: Reverse

Name: selB [H]

Synonym: APL_1561

Alternate gene names: 126209025

Gene position: 1787282-1785447 (Counterclockwise)

Preceding gene: 126209026

Following gene: 126209024

Centisome position: 78.58

GC content: 41.61

Gene sequence:

>1836_bases
ATGATTTTTGTTACCAGCGGACACGTGGATCATGGTAAAACGACCTTGTTACAAGCCTTAACAGGTAACGATACCGCTCA
TTTACCGGAAGAAAAAAAGCGTGGTTTAACGATTGATTTGGGCTATGCTTACTTGCCGATTGAAAATGATGTGTTAGGTT
TTATTGATGTGCCGGGGCATCAGAAATTCCTGTCAAACATGCTTGCCGGACTGGGCGGTGTACAAAATGCGTTATTGATT
GTGTCGGCGGAAGAAGGGATTAAGCCGCAAACGGCGGAACATTTAGAGATTCTGCGTTTATTGAATTTTACCAATATCTT
AGTGATTTTGACGAAAGCGGATCGCGTGGCGGCGGAGCAAGCCGAACAACTTATGACGAAAATTAAAGCGACTTATTCGT
TTCTATCCGACGCACCGTTTTTCTTGACCTCTGCGGTAACCGGACAAGGTATAGAAGCATTAAGAAATCAATTAATTCAG
TTAAGCCGACAACAATCACATCTTGAAAAACCGTTTAGATATGCGATTGACCGAGTATTTAATATTAAAGGTGCCGGTTT
GGTCGTAACCGGAACGGCGGTGGCGGGTAAAGTTAAAATCGGTGACGAGTTTTATTTATCAAGCGGTCAAAAAGTTAGAA
TTAAGCAAATTCACGCACAAAATCAGACGGCGGAAATCGGTACGGCAGGGCAACGTTTAGCTTTGAATCTTGCCAACGCG
GAAAAAGAGCAGATACAGCGCGGTGATTGGATTACCGAGTTGATGCCGCAGTTTACCGATAGAATTACCGTTAAAATGCA
TACGGCACAAAACCTAAAAGAAAATCATATTGTGCATTTATACCATTTTGCTTCGCACGTCACCGGCAAGTTGAATCTCT
TAACCGAAAAACAAGCGGTTAAAAACAGTGAAACTTTTGCAGAAATTATTTTCGATGCGCCGTTAGCAATGGCGGTGACG
GATAAACTGATTATTCGTAGTGGCGATGACAGCCAAACCTTAGCAGGAGCGGAAGTTTTAGAAATTGATTCGCCGAAACG
CTATAAGCGAACAGAACAACGCCTTGCCTTAGTAAAAGAGTTGGCAAAAACGACCGCTTATATGCAGAGAATCCCGCTTT
ACTTAAAGAATAGAGCGGAACAGCTGGATTCGTTGTTATGGCGTGAGCAATGTTTTGCACAAGAGTTGGCTGAACAGGGT
TTCGACGTGCAGTCGAAATGGATTTTTACCGCAGACTTTAAGCAACAAATTCAACAAAGAATTATCGAAAAACTCAGTGA
ATATCATCAAAATCATAATGATCAATTAGGCGTAACCAAAGCGAGATTATATCGTATCGCCGTGTTAGATCAGCCGGAAC
CGCTGGCTTATCAATTTATTGACGACTTAATCGAAAGTAAACAACTGGTACAAACCCGAGGTTGGATTCATTTACCGAAT
CATCGTATCGAATTTTCGGCAGAGGAATTGCAACTTTGGCAACAAATTCGACCGCTATTCGAAAGCAAAAGCGAAGCGCT
TTGGGTTCGTGATATTGCCACGGAATTATGTTGCGACGAAACCGAAATGCGAAACTTATTATATAAAGCCGGAAAATTAG
GCTACTTAATCCCGATTGTGAAAGATCGCTTCTTATTAAGCGAGAGAATGACTGAATTGGCTCAATTAGTAAAAGAGATT
GTGGAACAAAACGGTTCGGTATCGGTAAATCAGTTGCGTGATGAGATTCAGTGGGGACGTAAATTGACCGTTCAGCTGAT
TGAATATTTTGACCGTTCGGGCTTTTTAAGACGAAAAGGTAATATTCATTTATTACGTGATCAAGATACCTTCTAA

Upstream 100 bases:

>100_bases
TGCCAAGTCCGATAATCGGGCGATTTGCTCAGCAAAAATTTTGGTTGGACGTGCGTTCGGCGGCACAGTTTGAACAGTTG
TTAAATATGTTGGAGGAAGC

Downstream 100 bases:

>100_bases
TAAGAATACATTAATGAAGAAACAAAACGCACGTGCGGTTGCCGCACAAATTATTTTACAAGTGTTGGATCAAGGTAAAT
CGCTTGCCACTTTGATTCCT

Product: selenocysteine-specific elongation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MIFVTSGHVDHGKTTLLQALTGNDTAHLPEEKKRGLTIDLGYAYLPIENDVLGFIDVPGHQKFLSNMLAGLGGVQNALLI
VSAEEGIKPQTAEHLEILRLLNFTNILVILTKADRVAAEQAEQLMTKIKATYSFLSDAPFFLTSAVTGQGIEALRNQLIQ
LSRQQSHLEKPFRYAIDRVFNIKGAGLVVTGTAVAGKVKIGDEFYLSSGQKVRIKQIHAQNQTAEIGTAGQRLALNLANA
EKEQIQRGDWITELMPQFTDRITVKMHTAQNLKENHIVHLYHFASHVTGKLNLLTEKQAVKNSETFAEIIFDAPLAMAVT
DKLIIRSGDDSQTLAGAEVLEIDSPKRYKRTEQRLALVKELAKTTAYMQRIPLYLKNRAEQLDSLLWREQCFAQELAEQG
FDVQSKWIFTADFKQQIQQRIIEKLSEYHQNHNDQLGVTKARLYRIAVLDQPEPLAYQFIDDLIESKQLVQTRGWIHLPN
HRIEFSAEELQLWQQIRPLFESKSEALWVRDIATELCCDETEMRNLLYKAGKLGYLIPIVKDRFLLSERMTELAQLVKEI
VEQNGSVSVNQLRDEIQWGRKLTVQLIEYFDRSGFLRRKGNIHLLRDQDTF

Sequences:

>Translated_611_residues
MIFVTSGHVDHGKTTLLQALTGNDTAHLPEEKKRGLTIDLGYAYLPIENDVLGFIDVPGHQKFLSNMLAGLGGVQNALLI
VSAEEGIKPQTAEHLEILRLLNFTNILVILTKADRVAAEQAEQLMTKIKATYSFLSDAPFFLTSAVTGQGIEALRNQLIQ
LSRQQSHLEKPFRYAIDRVFNIKGAGLVVTGTAVAGKVKIGDEFYLSSGQKVRIKQIHAQNQTAEIGTAGQRLALNLANA
EKEQIQRGDWITELMPQFTDRITVKMHTAQNLKENHIVHLYHFASHVTGKLNLLTEKQAVKNSETFAEIIFDAPLAMAVT
DKLIIRSGDDSQTLAGAEVLEIDSPKRYKRTEQRLALVKELAKTTAYMQRIPLYLKNRAEQLDSLLWREQCFAQELAEQG
FDVQSKWIFTADFKQQIQQRIIEKLSEYHQNHNDQLGVTKARLYRIAVLDQPEPLAYQFIDDLIESKQLVQTRGWIHLPN
HRIEFSAEELQLWQQIRPLFESKSEALWVRDIATELCCDETEMRNLLYKAGKLGYLIPIVKDRFLLSERMTELAQLVKEI
VEQNGSVSVNQLRDEIQWGRKLTVQLIEYFDRSGFLRRKGNIHLLRDQDTF
>Mature_611_residues
MIFVTSGHVDHGKTTLLQALTGNDTAHLPEEKKRGLTIDLGYAYLPIENDVLGFIDVPGHQKFLSNMLAGLGGVQNALLI
VSAEEGIKPQTAEHLEILRLLNFTNILVILTKADRVAAEQAEQLMTKIKATYSFLSDAPFFLTSAVTGQGIEALRNQLIQ
LSRQQSHLEKPFRYAIDRVFNIKGAGLVVTGTAVAGKVKIGDEFYLSSGQKVRIKQIHAQNQTAEIGTAGQRLALNLANA
EKEQIQRGDWITELMPQFTDRITVKMHTAQNLKENHIVHLYHFASHVTGKLNLLTEKQAVKNSETFAEIIFDAPLAMAVT
DKLIIRSGDDSQTLAGAEVLEIDSPKRYKRTEQRLALVKELAKTTAYMQRIPLYLKNRAEQLDSLLWREQCFAQELAEQG
FDVQSKWIFTADFKQQIQQRIIEKLSEYHQNHNDQLGVTKARLYRIAVLDQPEPLAYQFIDDLIESKQLVQTRGWIHLPN
HRIEFSAEELQLWQQIRPLFESKSEALWVRDIATELCCDETEMRNLLYKAGKLGYLIPIVKDRFLLSERMTELAQLVKEI
VEQNGSVSVNQLRDEIQWGRKLTVQLIEYFDRSGFLRRKGNIHLLRDQDTF

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=276, Percent_Identity=28.6231884057971, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI223555963, Length=305, Percent_Identity=26.8852459016393, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI5729864, Length=305, Percent_Identity=26.8852459016393, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI82546879, Length=231, Percent_Identity=25.974025974026, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI46094014, Length=305, Percent_Identity=24.2622950819672, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI194018520, Length=305, Percent_Identity=23.6065573770492, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI194097354, Length=305, Percent_Identity=23.6065573770492, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI194018522, Length=305, Percent_Identity=23.6065573770492, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI2367247, Length=622, Percent_Identity=43.0868167202572, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1790412, Length=278, Percent_Identity=29.8561151079137, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789737, Length=278, Percent_Identity=29.8561151079137, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789108, Length=303, Percent_Identity=25.4125412541254, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1789559, Length=90, Percent_Identity=35.5555555555556, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=316, Percent_Identity=29.1139240506329, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25141371, Length=292, Percent_Identity=27.7397260273973, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506081, Length=259, Percent_Identity=27.7992277992278, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115532067, Length=308, Percent_Identity=26.2987012987013, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115532065, Length=308, Percent_Identity=26.2987012987013, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17565438, Length=226, Percent_Identity=27.8761061946903, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI32566629, Length=308, Percent_Identity=23.3766233766234, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=297, Percent_Identity=29.6296296296296, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325337, Length=261, Percent_Identity=25.6704980842912, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319594, Length=261, Percent_Identity=25.6704980842912, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363316, Length=277, Percent_Identity=28.5198555956679, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864358, Length=277, Percent_Identity=28.5198555956679, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922690, Length=264, Percent_Identity=31.8181818181818, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19921738, Length=275, Percent_Identity=28.7272727272727, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI45550900, Length=319, Percent_Identity=24.1379310344828, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922512, Length=320, Percent_Identity=25.625, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24654852, Length=320, Percent_Identity=25.625, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 69580; Mature: 69580

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFVTSGHVDHGKTTLLQALTGNDTAHLPEEKKRGLTIDLGYAYLPIENDVLGFIDVPGH
CEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHCCEEEEECEEEEEECCCCEEEEECCCH
QKFLSNMLAGLGGVQNALLIVSAEEGIKPQTAEHLEILRLLNFTNILVILTKADRVAAEQ
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHH
AEQLMTKIKATYSFLSDAPFFLTSAVTGQGIEALRNQLIQLSRQQSHLEKPFRYAIDRVF
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIKGAGLVVTGTAVAGKVKIGDEFYLSSGQKVRIKQIHAQNQTAEIGTAGQRLALNLANA
CCCCCCEEEEECEEEEEEEECCHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCC
EKEQIQRGDWITELMPQFTDRITVKMHTAQNLKENHIVHLYHFASHVTGKLNLLTEKQAV
CHHHHHCCCHHHHHHHHHHCCEEEEEECHHCCCCCCEEEEEEHHHHCCCCEEEEEHHHHH
KNSETFAEIIFDAPLAMAVTDKLIIRSGDDSQTLAGAEVLEIDSPKRYKRTEQRLALVKE
CCHHHHHHHHHCCCHHHHHHCCCEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
LAKTTAYMQRIPLYLKNRAEQLDSLLWREQCFAQELAEQGFDVQSKWIFTADFKQQIQQR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHH
IIEKLSEYHQNHNDQLGVTKARLYRIAVLDQPEPLAYQFIDDLIESKQLVQTRGWIHLPN
HHHHHHHHHCCCCCCCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCC
HRIEFSAEELQLWQQIRPLFESKSEALWVRDIATELCCDETEMRNLLYKAGKLGYLIPIV
CCEECCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCEEHHHH
KDRFLLSERMTELAQLVKEIVEQNGSVSVNQLRDEIQWGRKLTVQLIEYFDRSGFLRRKG
HHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
NIHLLRDQDTF
CEEEEECCCCC
>Mature Secondary Structure
MIFVTSGHVDHGKTTLLQALTGNDTAHLPEEKKRGLTIDLGYAYLPIENDVLGFIDVPGH
CEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHCCEEEEECEEEEEECCCCEEEEECCCH
QKFLSNMLAGLGGVQNALLIVSAEEGIKPQTAEHLEILRLLNFTNILVILTKADRVAAEQ
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHH
AEQLMTKIKATYSFLSDAPFFLTSAVTGQGIEALRNQLIQLSRQQSHLEKPFRYAIDRVF
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIKGAGLVVTGTAVAGKVKIGDEFYLSSGQKVRIKQIHAQNQTAEIGTAGQRLALNLANA
CCCCCCEEEEECEEEEEEEECCHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCC
EKEQIQRGDWITELMPQFTDRITVKMHTAQNLKENHIVHLYHFASHVTGKLNLLTEKQAV
CHHHHHCCCHHHHHHHHHHCCEEEEEECHHCCCCCCEEEEEEHHHHCCCCEEEEEHHHHH
KNSETFAEIIFDAPLAMAVTDKLIIRSGDDSQTLAGAEVLEIDSPKRYKRTEQRLALVKE
CCHHHHHHHHHCCCHHHHHHCCCEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
LAKTTAYMQRIPLYLKNRAEQLDSLLWREQCFAQELAEQGFDVQSKWIFTADFKQQIQQR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHH
IIEKLSEYHQNHNDQLGVTKARLYRIAVLDQPEPLAYQFIDDLIESKQLVQTRGWIHLPN
HHHHHHHHHCCCCCCCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCC
HRIEFSAEELQLWQQIRPLFESKSEALWVRDIATELCCDETEMRNLLYKAGKLGYLIPIV
CCEECCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCEEHHHH
KDRFLLSERMTELAQLVKEIVEQNGSVSVNQLRDEIQWGRKLTVQLIEYFDRSGFLRRKG
HHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
NIHLLRDQDTF
CEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]