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The map label for this gene is hemL

Identifier: 126209019

GI number: 126209019

Start: 1778004

End: 1779284

Strand: Direct

Name: hemL

Synonym: APL_1555

Alternate gene names: 126209019

Gene position: 1778004-1779284 (Clockwise)

Preceding gene: 126209011

Following gene: 126209022

Centisome position: 78.17

GC content: 46.37

Gene sequence:

>1281_bases
ATGTCTAAATCAGAACAACTGTTTGAAAAAGCTCAAAAAGTTATCCCGGGCGGGGTCAATTCACCGGTGCGTGCGTTTAA
AGGCGTAGGCGGTACGCCGGTATTTATCCAAAAAGCGGAAGGTGCGTATATTACCGATTCGGACGGTAAAAAATATATCG
ATTACGTCGGTTCTTGGGGACCGATGGTATTAGGTCATAATCACCCGGCAATTATTGATGCGGTATTGAAAGCGGTACCG
AACGGTTTAAGTTTCGGTGCGCCGACCGAAAGCGAAATCACTCTCGCTGAATTGGTTACTAAATTAGTCCCTTCTATCGA
ATTAGTCCGTATGGTCAGCTCGGGTACCGAAGCGACCATGTCGGCAATTCGTTTAGCACGCGGTTATACCGGTCGTGACA
AAATTATTAAATTTGAAGGCTGTTACCACGGACATTCCGATTCGCTATTAGTAAAAGCCGGTTCCGGTGCATTAACCCTA
GGTCAGCCGAGCGGTCCGGGTGTACCTGCCGATTTTGCGAAGCACACGCTCACTTGTACTTACAACGATTTAGATTCGGT
AAAAACCGCTTTCGAACAATATCCGAATGAAATTGCCTGTTTAATCGTTGAGCCTGTTGCCGGCAATATGAACTGTATTC
CGCCGAAAAATGATTTCTTAAAAGGTTTACGTGCTTTATGTGATCAATACGGTGCGGTGTTTATTATCGATGAAGTGATG
ACCGGTTTCCGTGTCGCATTAGGCGGTGCGCAAGCCTATTACGATGTTAAACCGGATTTAACCACACTCGGTAAAATTAT
CGGCGGCGGTATGCCGGTTGGTGCGTTTGGCGGTAAAAAAGAGATTATGGAATACATTGCACCGACAGGGCCGGTATATC
AGGCGGGTACGCTTTCAGGTAACCCGATTGCGATGGCGGCCGGTTTAGCTTGTTTAACCGAACTCTCCAAAGCGGGCAAT
GAAGAGAAACTTGCCGCACAAACTAAAACATTGGCGGAAGGTTTTAAAGCATTAGCGGATAAACATAACGTACTCTTTAC
CGCACAATATGTCGGCGGTATGTTCGGTTTATTCTTTACCGAGCAGGCGGAAATCACGAATTTCCAAGAGGTAATGAAAT
GCGATGCGGCGAAATTCAACCGCTTCTTCCATTTAATGTTGGAACAAGGCGTGTATCTTGCGCCAAGCGCATTTGAAGCG
GGCTTTATGTCGCTTGCCCATAGCGATGAAGATATTCAAGCGACTCTAGCAGCGGCGGACAAAGCCTTTGCTCAATTATA
A

Upstream 100 bases:

>100_bases
GAAAAGTAACCAAAAACAAGCGGTTATTTTTAAACTATTTTTTGTAAAGCGGAACCTTGTCGATCAACGCTATTCAGAAT
TTCAATTTAAGGAATCATTT

Downstream 100 bases:

>100_bases
TTAACGCCTTTATCCGCCCTCAAGCGACGATTCGTGTTGAGGGCGGATTGCCATTTAAACTAAAAAGATATACGGCAAAA
TATTCATAATAGCGGCGGTA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSKSEQLFEKAQKVIPGGVNSPVRAFKGVGGTPVFIQKAEGAYITDSDGKKYIDYVGSWGPMVLGHNHPAIIDAVLKAVP
NGLSFGAPTESEITLAELVTKLVPSIELVRMVSSGTEATMSAIRLARGYTGRDKIIKFEGCYHGHSDSLLVKAGSGALTL
GQPSGPGVPADFAKHTLTCTYNDLDSVKTAFEQYPNEIACLIVEPVAGNMNCIPPKNDFLKGLRALCDQYGAVFIIDEVM
TGFRVALGGAQAYYDVKPDLTTLGKIIGGGMPVGAFGGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGN
EEKLAAQTKTLAEGFKALADKHNVLFTAQYVGGMFGLFFTEQAEITNFQEVMKCDAAKFNRFFHLMLEQGVYLAPSAFEA
GFMSLAHSDEDIQATLAAADKAFAQL

Sequences:

>Translated_426_residues
MSKSEQLFEKAQKVIPGGVNSPVRAFKGVGGTPVFIQKAEGAYITDSDGKKYIDYVGSWGPMVLGHNHPAIIDAVLKAVP
NGLSFGAPTESEITLAELVTKLVPSIELVRMVSSGTEATMSAIRLARGYTGRDKIIKFEGCYHGHSDSLLVKAGSGALTL
GQPSGPGVPADFAKHTLTCTYNDLDSVKTAFEQYPNEIACLIVEPVAGNMNCIPPKNDFLKGLRALCDQYGAVFIIDEVM
TGFRVALGGAQAYYDVKPDLTTLGKIIGGGMPVGAFGGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGN
EEKLAAQTKTLAEGFKALADKHNVLFTAQYVGGMFGLFFTEQAEITNFQEVMKCDAAKFNRFFHLMLEQGVYLAPSAFEA
GFMSLAHSDEDIQATLAAADKAFAQL
>Mature_425_residues
SKSEQLFEKAQKVIPGGVNSPVRAFKGVGGTPVFIQKAEGAYITDSDGKKYIDYVGSWGPMVLGHNHPAIIDAVLKAVPN
GLSFGAPTESEITLAELVTKLVPSIELVRMVSSGTEATMSAIRLARGYTGRDKIIKFEGCYHGHSDSLLVKAGSGALTLG
QPSGPGVPADFAKHTLTCTYNDLDSVKTAFEQYPNEIACLIVEPVAGNMNCIPPKNDFLKGLRALCDQYGAVFIIDEVMT
GFRVALGGAQAYYDVKPDLTTLGKIIGGGMPVGAFGGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNE
EKLAAQTKTLAEGFKALADKHNVLFTAQYVGGMFGLFFTEQAEITNFQEVMKCDAAKFNRFFHLMLEQGVYLAPSAFEAG
FMSLAHSDEDIQATLAAADKAFAQL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=313, Percent_Identity=32.2683706070287, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI13994255, Length=361, Percent_Identity=25.4847645429363, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI284507298, Length=231, Percent_Identity=32.9004329004329, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=71.5962441314554, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=381, Percent_Identity=28.8713910761155, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787560, Length=283, Percent_Identity=33.9222614840989, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789759, Length=328, Percent_Identity=30.1829268292683, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1788044, Length=283, Percent_Identity=32.1554770318021, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI145693181, Length=349, Percent_Identity=28.9398280802292, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786991, Length=390, Percent_Identity=27.6923076923077, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=360, Percent_Identity=28.6111111111111, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25144271, Length=314, Percent_Identity=29.9363057324841, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32564660, Length=350, Percent_Identity=28.2857142857143, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=159, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=334, Percent_Identity=29.0419161676647, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=337, Percent_Identity=25.8160237388724, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357415, Length=304, Percent_Identity=29.6052631578947, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356575, Length=346, Percent_Identity=25.4335260115607, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_ACTP2 (A3N2K3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054244.1
- ProteinModelPortal:   A3N2K3
- SMR:   A3N2K3
- STRING:   A3N2K3
- GeneID:   4850218
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1555
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- PhylomeDB:   A3N2K3
- ProtClustDB:   PRK00062
- BioCyc:   APLE416269:APL_1555-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45267; Mature: 45136

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSEQLFEKAQKVIPGGVNSPVRAFKGVGGTPVFIQKAEGAYITDSDGKKYIDYVGSWG
CCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEECCCCEEECCCCCHHHHHHCCCC
PMVLGHNHPAIIDAVLKAVPNGLSFGAPTESEITLAELVTKLVPSIELVRMVSSGTEATM
CEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHCHHHHHHHHHCCHHHHH
SAIRLARGYTGRDKIIKFEGCYHGHSDSLLVKAGSGALTLGQPSGPGVPADFAKHTLTCT
HHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCHHHHCCEEEEE
YNDLDSVKTAFEQYPNEIACLIVEPVAGNMNCIPPKNDFLKGLRALCDQYGAVFIIDEVM
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVALGGAQAYYDVKPDLTTLGKIIGGGMPVGAFGGKKEIMEYIAPTGPVYQAGTLSG
HHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGLACLTELSKAGNEEKLAAQTKTLAEGFKALADKHNVLFTAQYVGGMFGLFFT
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCC
EQAEITNFQEVMKCDAAKFNRFFHLMLEQGVYLAPSAFEAGFMSLAHSDEDIQATLAAAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHH
KAFAQL
HHHHCC
>Mature Secondary Structure 
SKSEQLFEKAQKVIPGGVNSPVRAFKGVGGTPVFIQKAEGAYITDSDGKKYIDYVGSWG
CCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEECCCCEEECCCCCHHHHHHCCCC
PMVLGHNHPAIIDAVLKAVPNGLSFGAPTESEITLAELVTKLVPSIELVRMVSSGTEATM
CEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHCHHHHHHHHHCCHHHHH
SAIRLARGYTGRDKIIKFEGCYHGHSDSLLVKAGSGALTLGQPSGPGVPADFAKHTLTCT
HHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCHHHHCCEEEEE
YNDLDSVKTAFEQYPNEIACLIVEPVAGNMNCIPPKNDFLKGLRALCDQYGAVFIIDEVM
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVALGGAQAYYDVKPDLTTLGKIIGGGMPVGAFGGKKEIMEYIAPTGPVYQAGTLSG
HHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGLACLTELSKAGNEEKLAAQTKTLAEGFKALADKHNVLFTAQYVGGMFGLFFT
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCC
EQAEITNFQEVMKCDAAKFNRFFHLMLEQGVYLAPSAFEAGFMSLAHSDEDIQATLAAAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHH
KAFAQL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA