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The map label for this gene is tilS

Identifier: 126209005

GI number: 126209005

Start: 1754984

End: 1756243

Strand: Direct

Name: tilS

Synonym: APL_1541

Alternate gene names: 126209005

Gene position: 1754984-1756243 (Clockwise)

Preceding gene: 126209000

Following gene: 126209010

Centisome position: 77.16

GC content: 43.1

Gene sequence:

>1260_bases
ATGTTACTGGATACACTTCAAACACAGTTTAGGCACTATTTCCCGACTCAGCGAAATTTTGTGCTTGGCTTAAGCGGTGG
GATTGATTCGATTGTGTTATTGCACCTGTTGGCCGAATTACAGCTCAACCTAAGAGCGGTACATATTCACCACGGGCTTA
GTCCGAATGCCGACAATTGGGCTGCTTTTTGTGAACAAGTTTGCAAGCGGTTAAAAATTCCGTTTATTTTGCAAAAAGTC
ACGGTAGATCGCAGTGAAGGTATCGAAGCCGGCGCCAGAGCGGCACGTTATCAAGCAATCGGTGAAATTATTCAGCCTAA
CGAAGTGTTAGTGACCGCTCATCATCTTGATGATCAAACAGAAACTTTCTTGCTTGCCTTGAAACGTGGCAGTGGAATCA
AAGGGTTGTCAGCGATGCAAGCTGTCGGATTTTGGCAAAATTTTACCATTTTTAGACCGCTTCTTAACGTGAGCAAAGCG
CAAATCGAACAATATGCGCTGCAACAACAATTCACTTGGATTGAAGACGAAAGTAATCACGACAGCCATTACGACCGTAA
TTTTTTACGCAATGAGGTGTTACCGATCGTGAATCAACGCTGGCAGCATTTCAGTCAAATGGTAGCCCGTTCTGCGCAAC
ATTGTGCCGAACAACAAATGCTATTGGAAGAATTGCTTGCTCAAGAATTGCAACGCTATGCGGATTTTTCGGAAAAGCGT
TTAAATATCGAAGCTTTTCCTCAATTTTCTTTGGCGAAACAACAACAATTGATCCGTTTATGGCTGGAAAAATGCGGCGC
ACAAATGCCAAGTACGGCACAGCTAATGCAAATAATTCAACAAACAATTTATGCGGACGTCGATAAAAATCCGCAGCTTA
AATTAGCCGATTTTTGGTTGCGCCGTTATCAACATCATCTCTATTTAACCGGTGAATTGCTTGAACCGGATGATTTTTGC
CAGCCGCTCTTTGCCCAACAATCGCTAACGCTACCGGACGGAATCGGTGAGCTACAACATTTAGGCGACAGCATTATTTA
CCAAAAATCTGGCAAAATTGACCGCTTGTTATTACCGAAAGCGTTAGTAAATGCTCCGTTGCAAGTAAAGTTGACGCATC
AAGGTAAAGTCAAACAGTATGGCAAACCGATGCGAGAAGAAATGAAGAAACGCTACCAACAAGCGCAAGTTCCGGTTTGG
CTACGCAAACGCACACCGCTTATTTTTTTCCACGATCAGTTAGTTTTTATATGCCATTAA

Upstream 100 bases:

>100_bases
AATTTTAACATATTAAATCCGGCTCTCACTGCTCTTTTTCAAAAAGGGGCGCGGAATAATGATAGAATGAAGAAAATGAC
CGACACAGATAAGGCGCAAT

Downstream 100 bases:

>100_bases
AAAACCTGCTTTCGCAGGTTTATAAATTATAAAGATTGGATGATATACCCCAGTAAAGCATAAAATCCGATAAAAGAAAG
CATCAATAAGCCTCTGGCTA

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLLDTLQTQFRHYFPTQRNFVLGLSGGIDSIVLLHLLAELQLNLRAVHIHHGLSPNADNWAAFCEQVCKRLKIPFILQKV
TVDRSEGIEAGARAARYQAIGEIIQPNEVLVTAHHLDDQTETFLLALKRGSGIKGLSAMQAVGFWQNFTIFRPLLNVSKA
QIEQYALQQQFTWIEDESNHDSHYDRNFLRNEVLPIVNQRWQHFSQMVARSAQHCAEQQMLLEELLAQELQRYADFSEKR
LNIEAFPQFSLAKQQQLIRLWLEKCGAQMPSTAQLMQIIQQTIYADVDKNPQLKLADFWLRRYQHHLYLTGELLEPDDFC
QPLFAQQSLTLPDGIGELQHLGDSIIYQKSGKIDRLLLPKALVNAPLQVKLTHQGKVKQYGKPMREEMKKRYQQAQVPVW
LRKRTPLIFFHDQLVFICH

Sequences:

>Translated_419_residues
MLLDTLQTQFRHYFPTQRNFVLGLSGGIDSIVLLHLLAELQLNLRAVHIHHGLSPNADNWAAFCEQVCKRLKIPFILQKV
TVDRSEGIEAGARAARYQAIGEIIQPNEVLVTAHHLDDQTETFLLALKRGSGIKGLSAMQAVGFWQNFTIFRPLLNVSKA
QIEQYALQQQFTWIEDESNHDSHYDRNFLRNEVLPIVNQRWQHFSQMVARSAQHCAEQQMLLEELLAQELQRYADFSEKR
LNIEAFPQFSLAKQQQLIRLWLEKCGAQMPSTAQLMQIIQQTIYADVDKNPQLKLADFWLRRYQHHLYLTGELLEPDDFC
QPLFAQQSLTLPDGIGELQHLGDSIIYQKSGKIDRLLLPKALVNAPLQVKLTHQGKVKQYGKPMREEMKKRYQQAQVPVW
LRKRTPLIFFHDQLVFICH
>Mature_419_residues
MLLDTLQTQFRHYFPTQRNFVLGLSGGIDSIVLLHLLAELQLNLRAVHIHHGLSPNADNWAAFCEQVCKRLKIPFILQKV
TVDRSEGIEAGARAARYQAIGEIIQPNEVLVTAHHLDDQTETFLLALKRGSGIKGLSAMQAVGFWQNFTIFRPLLNVSKA
QIEQYALQQQFTWIEDESNHDSHYDRNFLRNEVLPIVNQRWQHFSQMVARSAQHCAEQQMLLEELLAQELQRYADFSEKR
LNIEAFPQFSLAKQQQLIRLWLEKCGAQMPSTAQLMQIIQQTIYADVDKNPQLKLADFWLRRYQHHLYLTGELLEPDDFC
QPLFAQQSLTLPDGIGELQHLGDSIIYQKSGKIDRLLLPKALVNAPLQVKLTHQGKVKQYGKPMREEMKKRYQQAQVPVW
LRKRTPLIFFHDQLVFICH

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=407, Percent_Identity=41.7690417690418, Blast_Score=279, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_ACTP2 (A3N2I9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054230.1
- ProteinModelPortal:   A3N2I9
- SMR:   A3N2I9
- STRING:   A3N2I9
- GeneID:   4849337
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1541
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   KSAVGFF
- PhylomeDB:   A3N2I9
- ProtClustDB:   CLSK918732
- BioCyc:   APLE416269:APL_1541-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 48578; Mature: 48578

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDTLQTQFRHYFPTQRNFVLGLSGGIDSIVLLHLLAELQLNLRAVHIHHGLSPNADNW
CCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHH
AAFCEQVCKRLKIPFILQKVTVDRSEGIEAGARAARYQAIGEIIQPNEVLVTAHHLDDQT
HHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
ETFLLALKRGSGIKGLSAMQAVGFWQNFTIFRPLLNVSKAQIEQYALQQQFTWIEDESNH
HHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DSHYDRNFLRNEVLPIVNQRWQHFSQMVARSAQHCAEQQMLLEELLAQELQRYADFSEKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHC
LNIEAFPQFSLAKQQQLIRLWLEKCGAQMPSTAQLMQIIQQTIYADVDKNPQLKLADFWL
CCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RRYQHHLYLTGELLEPDDFCQPLFAQQSLTLPDGIGELQHLGDSIIYQKSGKIDRLLLPK
HHHHCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCHHEEECCCCCCHHCCCH
ALVNAPLQVKLTHQGKVKQYGKPMREEMKKRYQQAQVPVWLRKRTPLIFFHDQLVFICH
HHHCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCEEEEEC
>Mature Secondary Structure
MLLDTLQTQFRHYFPTQRNFVLGLSGGIDSIVLLHLLAELQLNLRAVHIHHGLSPNADNW
CCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHH
AAFCEQVCKRLKIPFILQKVTVDRSEGIEAGARAARYQAIGEIIQPNEVLVTAHHLDDQT
HHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
ETFLLALKRGSGIKGLSAMQAVGFWQNFTIFRPLLNVSKAQIEQYALQQQFTWIEDESNH
HHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DSHYDRNFLRNEVLPIVNQRWQHFSQMVARSAQHCAEQQMLLEELLAQELQRYADFSEKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHC
LNIEAFPQFSLAKQQQLIRLWLEKCGAQMPSTAQLMQIIQQTIYADVDKNPQLKLADFWL
CCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RRYQHHLYLTGELLEPDDFCQPLFAQQSLTLPDGIGELQHLGDSIIYQKSGKIDRLLLPK
HHHHCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCHHEEECCCCCCHHCCCH
ALVNAPLQVKLTHQGKVKQYGKPMREEMKKRYQQAQVPVWLRKRTPLIFFHDQLVFICH
HHHCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA