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The map label for this gene is dnaG [H]

Identifier: 126208938

GI number: 126208938

Start: 1686354

End: 1688096

Strand: Direct

Name: dnaG [H]

Synonym: APL_1474

Alternate gene names: 126208938

Gene position: 1686354-1688096 (Clockwise)

Preceding gene: 126208937

Following gene: 126208939

Centisome position: 74.14

GC content: 44.58

Gene sequence:

>1743_bases
ATGAAAGGCACAATTCCTAGTTCGTTTATTGATGATCTTGTTGCTCGTACCGACATTGTTGAACTCATCAATAGTCGAGT
CAAACTCAAAAAAGCAGGACGAGATTATCAAGCCTGTTGTCCGTTTCATCACGAAAAAAGTCCTTCGTTTACCGTCAGTC
AATCTAAACAGTTTTATCACTGCTTTGGTTGCGGTGCGCACGGTAATGCGATTAGCTTCTTGATGGATTACGATAAACTG
GAATTTCCCGAGGCGATTGAAGAACTTGCCGCGATGCAAGGTTTGGAAGTACCGCGTGAGAATGTGATAAACCGTGACGG
CAAGCCGCAAGCAAGCTACAAAACCAAGCGTAATTTATATGAGCTGTTAGAAGCGATTAGTCGGTTTTATCAACAAAATT
TAACCCAAGATATTCCTAGCCAAAGTTACCTACAAAACCGTGGACTGTCAGCGGAAATCATCGCTCGTTTTGAAATTGGT
TTTGCATATAATTCGATGGATTCCGTGCTACGTAAATTCGGCACGAATCGTGATGAAGTACAGAAATTGTTCGATACGGG
TATGATTACGCAAAATGATAGCGGTCGAATTTACGATAAATTTCGCAATAGAGTAATGTTTCCGATTCGTGATAAACGCG
GGCGGGTAATTGCCTTTGGTGGGCGAGTCATGGGGGATGAACGACCTAAATATCTGAATTCACCGGAATCAGCGACTTAT
CATAAAGGTAATGAACTGTATGGTTTATTTCAAGCGTTACAACAGAATGAAAATCCTACCTCACTCGTTGTGGTCGAAGG
GTATATGGATGTGGTCGCACTGGCGCAATTCGGGGTGGATAACGTGGTGGCGTCACTCGGTACGGCAACTACCGGCGAGC
AAATCCAGCAGATGTTCCGTGTCACCGAACAAGTGATTTGTTGCTATGACGGCGATAGAGCGGGGCGAGAGGCGGCATGG
CGGGCGTTTGAGAACGCATTACCGTATTTGCATGACGGACGACAACTGAAATTTATTTTCTTGCCGGACGGCGAAGATCC
GGACAGTTTTGTACGCGCACAGGGCAAGCAAGGCTTTGAAGCCTACTTGCAAAATGCGATGTCACTCAGTGATTTTCTAT
TCGATTCGCTTATTGCACAGGTTGATCTTTCAAGCAAAGAAGGTAAATCGAAACTGGCGGCATTAGCAATACCGTTGATT
AATCGTATTCCGGGTGAAATGCTGCGAGTTTATTTACGCAATATTCTCGGACAAAAGCTCGGTATTTTAGATCCGGCGCA
ATTGGAAGCAATGTTGCCGAGTCGTTCGCAAGCCGTACAAAAAAAGGCGGTGCAAACGCCGCAAATTAAGCGAACGCCGA
TGCGTTTATTAATCGCCTTATTATTGCAAAACCCCGAATTGGTTAAATTCGTGCCGGATATTTCCGCGCTTAAAACGCTG
GAAGAACCGGGCTTTGAATTATTACTTGAGCTGGTTGAGGTTTGCCGGCAAAAAGTCGGTGTCTCAATGGGCGCATTACT
CGAACATTGGCGAGATAAACCGAATTACCGCACTCTTGAACTGTTAGCCGATTGGGAACATTTGGTCACATCGGAAAACA
TCGAAACGACTTTTATTGAAACGCTTGATTTTCTTTATGCGAAATTGGTGGAAAAGCGAATCGAAGTATTAATTGCCAAA
GATCGCAGCCTAGGACTAAGCGCGGAAGAAAAGCAAGAGTTGGTCATGTTGATTGCACAATGA

Upstream 100 bases:

>100_bases
ACCCCTATGTAACCTATAGTAAAATAAGCGGTCAAATTTCAATAAATTTTGCAAAATTTAGCGTAAATCTAACCGCTTGC
CGCTATGACAAGGAGAATCA

Downstream 100 bases:

>100_bases
TTAAGCGGTCATTTTTGAGAAATTTTTTGCAAAACTATCAAGAAAATTTTAATTAATGGTATAATCCAATACTATTTTTG
CGCGCACACAAAACGGATAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MKGTIPSSFIDDLVARTDIVELINSRVKLKKAGRDYQACCPFHHEKSPSFTVSQSKQFYHCFGCGAHGNAISFLMDYDKL
EFPEAIEELAAMQGLEVPRENVINRDGKPQASYKTKRNLYELLEAISRFYQQNLTQDIPSQSYLQNRGLSAEIIARFEIG
FAYNSMDSVLRKFGTNRDEVQKLFDTGMITQNDSGRIYDKFRNRVMFPIRDKRGRVIAFGGRVMGDERPKYLNSPESATY
HKGNELYGLFQALQQNENPTSLVVVEGYMDVVALAQFGVDNVVASLGTATTGEQIQQMFRVTEQVICCYDGDRAGREAAW
RAFENALPYLHDGRQLKFIFLPDGEDPDSFVRAQGKQGFEAYLQNAMSLSDFLFDSLIAQVDLSSKEGKSKLAALAIPLI
NRIPGEMLRVYLRNILGQKLGILDPAQLEAMLPSRSQAVQKKAVQTPQIKRTPMRLLIALLLQNPELVKFVPDISALKTL
EEPGFELLLELVEVCRQKVGVSMGALLEHWRDKPNYRTLELLADWEHLVTSENIETTFIETLDFLYAKLVEKRIEVLIAK
DRSLGLSAEEKQELVMLIAQ

Sequences:

>Translated_580_residues
MKGTIPSSFIDDLVARTDIVELINSRVKLKKAGRDYQACCPFHHEKSPSFTVSQSKQFYHCFGCGAHGNAISFLMDYDKL
EFPEAIEELAAMQGLEVPRENVINRDGKPQASYKTKRNLYELLEAISRFYQQNLTQDIPSQSYLQNRGLSAEIIARFEIG
FAYNSMDSVLRKFGTNRDEVQKLFDTGMITQNDSGRIYDKFRNRVMFPIRDKRGRVIAFGGRVMGDERPKYLNSPESATY
HKGNELYGLFQALQQNENPTSLVVVEGYMDVVALAQFGVDNVVASLGTATTGEQIQQMFRVTEQVICCYDGDRAGREAAW
RAFENALPYLHDGRQLKFIFLPDGEDPDSFVRAQGKQGFEAYLQNAMSLSDFLFDSLIAQVDLSSKEGKSKLAALAIPLI
NRIPGEMLRVYLRNILGQKLGILDPAQLEAMLPSRSQAVQKKAVQTPQIKRTPMRLLIALLLQNPELVKFVPDISALKTL
EEPGFELLLELVEVCRQKVGVSMGALLEHWRDKPNYRTLELLADWEHLVTSENIETTFIETLDFLYAKLVEKRIEVLIAK
DRSLGLSAEEKQELVMLIAQ
>Mature_580_residues
MKGTIPSSFIDDLVARTDIVELINSRVKLKKAGRDYQACCPFHHEKSPSFTVSQSKQFYHCFGCGAHGNAISFLMDYDKL
EFPEAIEELAAMQGLEVPRENVINRDGKPQASYKTKRNLYELLEAISRFYQQNLTQDIPSQSYLQNRGLSAEIIARFEIG
FAYNSMDSVLRKFGTNRDEVQKLFDTGMITQNDSGRIYDKFRNRVMFPIRDKRGRVIAFGGRVMGDERPKYLNSPESATY
HKGNELYGLFQALQQNENPTSLVVVEGYMDVVALAQFGVDNVVASLGTATTGEQIQQMFRVTEQVICCYDGDRAGREAAW
RAFENALPYLHDGRQLKFIFLPDGEDPDSFVRAQGKQGFEAYLQNAMSLSDFLFDSLIAQVDLSSKEGKSKLAALAIPLI
NRIPGEMLRVYLRNILGQKLGILDPAQLEAMLPSRSQAVQKKAVQTPQIKRTPMRLLIALLLQNPELVKFVPDISALKTL
EEPGFELLLELVEVCRQKVGVSMGALLEHWRDKPNYRTLELLADWEHLVTSENIETTFIETLDFLYAKLVEKRIEVLIAK
DRSLGLSAEEKQELVMLIAQ

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=579, Percent_Identity=56.9948186528497, Blast_Score=690, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65640; Mature: 65640

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGTIPSSFIDDLVARTDIVELINSRVKLKKAGRDYQACCPFHHEKSPSFTVSQSKQFYH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEECCCCCEEE
CFGCGAHGNAISFLMDYDKLEFPEAIEELAAMQGLEVPRENVINRDGKPQASYKTKRNLY
EEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHH
ELLEAISRFYQQNLTQDIPSQSYLQNRGLSAEIIARFEIGFAYNSMDSVLRKFGTNRDEV
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHEEEHHHHHHHHHHHHHHHHCCCHHHH
QKLFDTGMITQNDSGRIYDKFRNRVMFPIRDKRGRVIAFGGRVMGDERPKYLNSPESATY
HHHHHCCCEEECCCCCHHHHHHCCEECEEECCCCCEEEECCEECCCCCCCCCCCCCCCCC
HKGNELYGLFQALQQNENPTSLVVVEGYMDVVALAQFGVDNVVASLGTATTGEQIQQMFR
CCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHH
VTEQVICCYDGDRAGREAAWRAFENALPYLHDGRQLKFIFLPDGEDPDSFVRAQGKQGFE
HHHHEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCHHH
AYLQNAMSLSDFLFDSLIAQVDLSSKEGKSKLAALAIPLINRIPGEMLRVYLRNILGQKL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
GILDPAQLEAMLPSRSQAVQKKAVQTPQIKRTPMRLLIALLLQNPELVKFVPDISALKTL
CCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHH
EEPGFELLLELVEVCRQKVGVSMGALLEHWRDKPNYRTLELLADWEHLVTSENIETTFIE
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHHHHH
TLDFLYAKLVEKRIEVLIAKDRSLGLSAEEKQELVMLIAQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEEEECC
>Mature Secondary Structure
MKGTIPSSFIDDLVARTDIVELINSRVKLKKAGRDYQACCPFHHEKSPSFTVSQSKQFYH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEECCCCCEEE
CFGCGAHGNAISFLMDYDKLEFPEAIEELAAMQGLEVPRENVINRDGKPQASYKTKRNLY
EEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHH
ELLEAISRFYQQNLTQDIPSQSYLQNRGLSAEIIARFEIGFAYNSMDSVLRKFGTNRDEV
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHEEEHHHHHHHHHHHHHHHHCCCHHHH
QKLFDTGMITQNDSGRIYDKFRNRVMFPIRDKRGRVIAFGGRVMGDERPKYLNSPESATY
HHHHHCCCEEECCCCCHHHHHHCCEECEEECCCCCEEEECCEECCCCCCCCCCCCCCCCC
HKGNELYGLFQALQQNENPTSLVVVEGYMDVVALAQFGVDNVVASLGTATTGEQIQQMFR
CCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHH
VTEQVICCYDGDRAGREAAWRAFENALPYLHDGRQLKFIFLPDGEDPDSFVRAQGKQGFE
HHHHEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCHHH
AYLQNAMSLSDFLFDSLIAQVDLSSKEGKSKLAALAIPLINRIPGEMLRVYLRNILGQKL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
GILDPAQLEAMLPSRSQAVQKKAVQTPQIKRTPMRLLIALLLQNPELVKFVPDISALKTL
CCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHH
EEPGFELLLELVEVCRQKVGVSMGALLEHWRDKPNYRTLELLADWEHLVTSENIETTFIE
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHHHHH
TLDFLYAKLVEKRIEVLIAKDRSLGLSAEEKQELVMLIAQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]