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The map label for this gene is napF [H]

Identifier: 126208895

GI number: 126208895

Start: 1636794

End: 1637324

Strand: Reverse

Name: napF [H]

Synonym: APL_1431

Alternate gene names: 126208895

Gene position: 1637324-1636794 (Counterclockwise)

Preceding gene: 126208897

Following gene: 126208894

Centisome position: 71.99

GC content: 44.82

Gene sequence:

>531_bases
TTGTCCGAGTTCAGCCAACAATCTTTACCTCGACGTAGTTTCTTACGCGGTCATTTTTTAAATGCGTTACAAACCGAGCA
GGTAAGGCAGCAAGGACATCATGCGATTCGCCCGCCTTGGACAAATCTTGCAGATTTTTATGAAAAATGCACCGCTTGCG
ATAATTGCATATCCGCCTGTGAAACACAAATTATTGTGAAAGGGGCGGGCGGTTATCCCGAGATTGATTTTAGTCGCGGC
GAATGTACTTTTTGTGAAGCTTGTGTGAAAAGTTGTGAGTCGGATGTGTTTTTGGCTACAACGGAAGAAGCATGGCGGCA
TAAGGTCGAAATTAAAGAAAACTGCTTATTAAAACAGCGGGTTGAATGTAGAAGTTGCGGAGACGGTTGTGAAAGCAGAG
CCATTCGTTTTCGCCCCGCATTGGGCGGCATTGCAACTTTGCAACTGGATTTAACCGCTTGTAACGGTTGTGGCGCTTGC
CTATCGGTTTGTCCTACTTCCGCAATATCAATTTTAAGGAATGAACTTTAG

Upstream 100 bases:

>100_bases
TTCCTTCGGTTCTTAGGCAAATACCTCGTTGGAGTGATGAAAGCGGATCAGTAAAAACCGATAATATTCATTATTTAGTT
TTCTTTGCCGTAGGTGTGTT

Downstream 100 bases:

>100_bases
CAATGAGTACGGAAAACTTAAACGAAGCGAAAAACTGGTATGTATGTAGCTTAGTCGTTCAAGCAAAACCAGCAAAATTA
GCGCAAGTGAAAGCGGACAT

Product: ferredoxin-type protein NapF

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MSEFSQQSLPRRSFLRGHFLNALQTEQVRQQGHHAIRPPWTNLADFYEKCTACDNCISACETQIIVKGAGGYPEIDFSRG
ECTFCEACVKSCESDVFLATTEEAWRHKVEIKENCLLKQRVECRSCGDGCESRAIRFRPALGGIATLQLDLTACNGCGAC
LSVCPTSAISILRNEL

Sequences:

>Translated_176_residues
MSEFSQQSLPRRSFLRGHFLNALQTEQVRQQGHHAIRPPWTNLADFYEKCTACDNCISACETQIIVKGAGGYPEIDFSRG
ECTFCEACVKSCESDVFLATTEEAWRHKVEIKENCLLKQRVECRSCGDGCESRAIRFRPALGGIATLQLDLTACNGCGAC
LSVCPTSAISILRNEL
>Mature_175_residues
SEFSQQSLPRRSFLRGHFLNALQTEQVRQQGHHAIRPPWTNLADFYEKCTACDNCISACETQIIVKGAGGYPEIDFSRGE
CTFCEACVKSCESDVFLATTEEAWRHKVEIKENCLLKQRVECRSCGDGCESRAIRFRPALGGIATLQLDLTACNGCGACL
SVCPTSAISILRNEL

Specific function: Involved in electron transfer [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=161, Percent_Identity=44.0993788819876, Blast_Score=140, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19488; Mature: 19357

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00014 ER_TARGET ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
9.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFSQQSLPRRSFLRGHFLNALQTEQVRQQGHHAIRPPWTNLADFYEKCTACDNCISAC
CCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ETQIIVKGAGGYPEIDFSRGECTFCEACVKSCESDVFLATTEEAWRHKVEIKENCLLKQR
HHEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHH
VECRSCGDGCESRAIRFRPALGGIATLQLDLTACNGCGACLSVCPTSAISILRNEL
HHHHHCCCCHHHCCEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEFSQQSLPRRSFLRGHFLNALQTEQVRQQGHHAIRPPWTNLADFYEKCTACDNCISAC
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ETQIIVKGAGGYPEIDFSRGECTFCEACVKSCESDVFLATTEEAWRHKVEIKENCLLKQR
HHEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHH
VECRSCGDGCESRAIRFRPALGGIATLQLDLTACNGCGACLSVCPTSAISILRNEL
HHHHHCCCCHHHCCEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]