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The map label for this gene is manX [H]

Identifier: 126208855

GI number: 126208855

Start: 1592366

End: 1593337

Strand: Direct

Name: manX [H]

Synonym: APL_1391

Alternate gene names: 126208855

Gene position: 1592366-1593337 (Clockwise)

Preceding gene: 126208854

Following gene: 126208856

Centisome position: 70.01

GC content: 44.96

Gene sequence:

>972_bases
ATGAGTATTGCTATTATCATAGCAACCCACGGTGTCGCCGCTGAACAGCTGCTCAAAACGACCGAGATGCTGATTGGTGA
ACAGTCCGATGTTGCTTATATAGATTTCGTACCCGGTGAGAATGCTGAAACCATTATGGGGAAATACCAAGCTAAACTCG
CTAACGAATTGGCACACTGTGATGAAGTATTATTTTTAGTCGATACATGGGGCGGCAGTCCGTTTAATGCGGCAAATCGA
ACTATCGACGGTAAACAAAATATGAATATCGTTACCGGTGTTAATGTGCCGATGTTAGTCGAAACCTTTATGGCGCGTGA
TGACGATCCGTCTTTAGATGAGTTAGTAGAAATCGCTTTAGAAGCCGGTCGTTTAGGCGTTCGAGCATTAAAAGAGCAAG
AACAAACGGCGGTAGAACCGGAAGTTAAACCCGCTCCGGTCGCTCAACCGACAACGGTACAAAATAACGGAGTCGGGCAT
TTAACAATCGGCTTGGCTCGTATTGACGACCGATTAATTCACGGACAAGTTGCAACACGTTGGACAAAGGAAAGCAGAGT
CACTCGCATCGTTGTCGTTAATGATGATGTGGCTAAAGATACGGTACGTTCAACCATGCTGAAAAGCGTTGCGCCTCCCG
GGGTAACCGCACACGTAGTACCGGTCGATAAAATGATTCGAGTGTATAACAATCCCGAATATGCCGGCGAACGTATGATG
TTACTTTTCACTAATCCGACCGATGTCGTACGTTTGTCGGATGCCGGCGTTACGTTCGAATCGATCAATATCGGCGGTAT
GGCGTATAAAGACGGTAAAAAAATGATAACTTCCGCAGTAGCGGTGGATGATCAAGACATTGATGCCTTCAAAACATTAG
ACGCTAAAGGCATTGAGCTGGATGTGCGTAAAGTGTCAAATGACGCTCGTCAGTATATGATGGATTTATTAAAGAAAAAC
AGCTTAATCTAA

Upstream 100 bases:

>100_bases
AATTCACAATTGTTTTGTGTAAATTTTCATCTAGAATTTACTCCGATTTTCACTCTATTTGAAAAAACAGTTTCTAAAAA
ATCCCATTAAGGAGTAAAAA

Downstream 100 bases:

>100_bases
AAGGAAACTAAAATGGAAATTTCAACTTTACAAGTCATTCTTGTCTTCCTCGTGTCCTGTATTTGCGGTGCGGGCTCTAT
TCTTGACGAATTCCAAACGC

Product: PTS system mannose-specific EIIAB component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSIAIIIATHGVAAEQLLKTTEMLIGEQSDVAYIDFVPGENAETIMGKYQAKLANELAHCDEVLFLVDTWGGSPFNAANR
TIDGKQNMNIVTGVNVPMLVETFMARDDDPSLDELVEIALEAGRLGVRALKEQEQTAVEPEVKPAPVAQPTTVQNNGVGH
LTIGLARIDDRLIHGQVATRWTKESRVTRIVVVNDDVAKDTVRSTMLKSVAPPGVTAHVVPVDKMIRVYNNPEYAGERMM
LLFTNPTDVVRLSDAGVTFESINIGGMAYKDGKKMITSAVAVDDQDIDAFKTLDAKGIELDVRKVSNDARQYMMDLLKKN
SLI

Sequences:

>Translated_323_residues
MSIAIIIATHGVAAEQLLKTTEMLIGEQSDVAYIDFVPGENAETIMGKYQAKLANELAHCDEVLFLVDTWGGSPFNAANR
TIDGKQNMNIVTGVNVPMLVETFMARDDDPSLDELVEIALEAGRLGVRALKEQEQTAVEPEVKPAPVAQPTTVQNNGVGH
LTIGLARIDDRLIHGQVATRWTKESRVTRIVVVNDDVAKDTVRSTMLKSVAPPGVTAHVVPVDKMIRVYNNPEYAGERMM
LLFTNPTDVVRLSDAGVTFESINIGGMAYKDGKKMITSAVAVDDQDIDAFKTLDAKGIELDVRKVSNDARQYMMDLLKKN
SLI
>Mature_322_residues
SIAIIIATHGVAAEQLLKTTEMLIGEQSDVAYIDFVPGENAETIMGKYQAKLANELAHCDEVLFLVDTWGGSPFNAANRT
IDGKQNMNIVTGVNVPMLVETFMARDDDPSLDELVEIALEAGRLGVRALKEQEQTAVEPEVKPAPVAQPTTVQNNGVGHL
TIGLARIDDRLIHGQVATRWTKESRVTRIVVVNDDVAKDTVRSTMLKSVAPPGVTAHVVPVDKMIRVYNNPEYAGERMML
LFTNPTDVVRLSDAGVTFESINIGGMAYKDGKKMITSAVAVDDQDIDAFKTLDAKGIELDVRKVSNDARQYMMDLLKKNS
LI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=321, Percent_Identity=62.9283489096573, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI87082217, Length=158, Percent_Identity=34.8101265822785, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789527, Length=152, Percent_Identity=30.9210526315789, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35294; Mature: 35163

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAIIIATHGVAAEQLLKTTEMLIGEQSDVAYIDFVPGENAETIMGKYQAKLANELAHC
CEEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
DEVLFLVDTWGGSPFNAANRTIDGKQNMNIVTGVNVPMLVETFMARDDDPSLDELVEIAL
HHEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHH
EAGRLGVRALKEQEQTAVEPEVKPAPVAQPTTVQNNGVGHLTIGLARIDDRLIHGQVATR
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEEEHHCCHHHCCHHHHH
WTKESRVTRIVVVNDDVAKDTVRSTMLKSVAPPGVTAHVVPVDKMIRVYNNPEYAGERMM
CCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCCEEE
LLFTNPTDVVRLSDAGVTFESINIGGMAYKDGKKMITSAVAVDDQDIDAFKTLDAKGIEL
EEEECCCCEEEEECCCCEEEEEECCCEEECCCHHHHHHHHCCCCCCCHHHHHCCCCCCEE
DVRKVSNDARQYMMDLLKKNSLI
EHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIAIIIATHGVAAEQLLKTTEMLIGEQSDVAYIDFVPGENAETIMGKYQAKLANELAHC
EEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
DEVLFLVDTWGGSPFNAANRTIDGKQNMNIVTGVNVPMLVETFMARDDDPSLDELVEIAL
HHEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHH
EAGRLGVRALKEQEQTAVEPEVKPAPVAQPTTVQNNGVGHLTIGLARIDDRLIHGQVATR
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEEEHHCCHHHCCHHHHH
WTKESRVTRIVVVNDDVAKDTVRSTMLKSVAPPGVTAHVVPVDKMIRVYNNPEYAGERMM
CCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCCEEE
LLFTNPTDVVRLSDAGVTFESINIGGMAYKDGKKMITSAVAVDDQDIDAFKTLDAKGIEL
EEEECCCCEEEEECCCCEEEEEECCCEEECCCHHHHHHHHCCCCCCCHHHHHCCCCCCEE
DVRKVSNDARQYMMDLLKKNSLI
EHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]