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The map label for this gene is lpxK

Identifier: 126208744

GI number: 126208744

Start: 1469348

End: 1470328

Strand: Reverse

Name: lpxK

Synonym: APL_1278

Alternate gene names: 126208744

Gene position: 1470328-1469348 (Counterclockwise)

Preceding gene: 126208745

Following gene: 126208743

Centisome position: 64.64

GC content: 43.83

Gene sequence:

>981_bases
ATGAATATTTGGCAATCTACCTCAATTATCACTTGGCTGCTTGCGCCGTTTTCATTGTTGTTTTGGCTGGTTAGTCAAAT
CCGCTTGTTTTTGTTTCGCAAAAAAATCTTAAAATCTTACCGCTCTCCGGTGCCGGTGTTAGTGGTCGGTAATATTTCAG
TGGGTGGTAACGGAAAAACGCCTGTGGTTGTGTGGCTTGTGGAACAGCTACAACAACGTGGTGTGAAAGTCGGTGTGATT
TCGCGCGGATATGGCGGCAAAAGTAAGGATTTTCCTCAATTAGTCACCAACCAGAGTTCAGCGGAAATGGTGGGCGATGA
ACCTGTGTTGATTGTGCAACGAACCGGTGTGCCGCTGGCAATTTCAGCTAATCGTCAGCAAAGTATCGAATTATTACTGA
ATCAATTTAAGCTGGATTTAATTGTTACCGATGACGGCTTGCAACATTACGCCTTACAGCGAGATATCGAATGGGTTGTG
GTTGATGGCATACGTCGTTTTGGTAACGGTTTTGTTTTGCCTGCCGGCGGCTTGCGTGAGCTGCCAAGCCGTTTACAATC
GGTACAAGCGATTATCTGTAATGGTGGAAAGGCTCAGCCGAATGAACATTTAATGACACTCGAGCCTGAATTTGCCGTTA
ATCTAAGAACGGGCGAGCAAAAGCCGATAACTGATTTTATCGGGCAAGAATGTGTGGCTATTGCCGGAATCGGGCATCCT
CCCCGATTTTTTAATATGCTTGAAAATTTAGGCGTTAAATTACTTAAAACACAAGGCTTTGCCGATCACCAAGCATTTGA
ACCAGCTCAACTTAAAGCGCTTGCAGCCGAACAAATTCCATTACTTATGACGGAAAAAGATGCGGTAAAATGCCGAACTT
TTGCTCAGCAAAATTGGTGGTATGTACCGGTTTCTGCAAAATTTTCTCCGGAATCGACTGCTTGTTTACTCGAACCGATT
TTAAAACGATTAGGAAAATAA

Upstream 100 bases:

>100_bases
AGAGTAAGCATCGCTAAAATGGACAATGTTCTTCATTGTCCATTTGTTTTATCTGTAGGCATCGCCGTTACGCAAACGAT
AGTATCTTTAAAGAAATTAT

Downstream 100 bases:

>100_bases
TATGAACGAAAAACTAATTAATCATCTTGCTTGTCCGGTAACCAATACCGGACTGGAATGGGATAAAGCCAATAATCGCT
TGATTAGCCGTGAAGCAAAA

Product: tetraacyldisaccharide 4'-kinase

Products: NA

Alternate protein names: Lipid A 4'-kinase

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNIWQSTSIITWLLAPFSLLFWLVSQIRLFLFRKKILKSYRSPVPVLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVI
SRGYGGKSKDFPQLVTNQSSAEMVGDEPVLIVQRTGVPLAISANRQQSIELLLNQFKLDLIVTDDGLQHYALQRDIEWVV
VDGIRRFGNGFVLPAGGLRELPSRLQSVQAIICNGGKAQPNEHLMTLEPEFAVNLRTGEQKPITDFIGQECVAIAGIGHP
PRFFNMLENLGVKLLKTQGFADHQAFEPAQLKALAAEQIPLLMTEKDAVKCRTFAQQNWWYVPVSAKFSPESTACLLEPI
LKRLGK

Sequences:

>Translated_326_residues
MNIWQSTSIITWLLAPFSLLFWLVSQIRLFLFRKKILKSYRSPVPVLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVI
SRGYGGKSKDFPQLVTNQSSAEMVGDEPVLIVQRTGVPLAISANRQQSIELLLNQFKLDLIVTDDGLQHYALQRDIEWVV
VDGIRRFGNGFVLPAGGLRELPSRLQSVQAIICNGGKAQPNEHLMTLEPEFAVNLRTGEQKPITDFIGQECVAIAGIGHP
PRFFNMLENLGVKLLKTQGFADHQAFEPAQLKALAAEQIPLLMTEKDAVKCRTFAQQNWWYVPVSAKFSPESTACLLEPI
LKRLGK
>Mature_326_residues
MNIWQSTSIITWLLAPFSLLFWLVSQIRLFLFRKKILKSYRSPVPVLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVI
SRGYGGKSKDFPQLVTNQSSAEMVGDEPVLIVQRTGVPLAISANRQQSIELLLNQFKLDLIVTDDGLQHYALQRDIEWVV
VDGIRRFGNGFVLPAGGLRELPSRLQSVQAIICNGGKAQPNEHLMTLEPEFAVNLRTGEQKPITDFIGQECVAIAGIGHP
PRFFNMLENLGVKLLKTQGFADHQAFEPAQLKALAAEQIPLLMTEKDAVKCRTFAQQNWWYVPVSAKFSPESTACLLEPI
LKRLGK

Specific function: Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1- P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA)

COG id: COG1663

COG function: function code M; Tetraacyldisaccharide-1-P 4'-kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxK family

Homologues:

Organism=Escherichia coli, GI1787144, Length=317, Percent_Identity=52.3659305993691, Blast_Score=328, Evalue=4e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXK_ACTP2 (A3N1S8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053969.1
- ProteinModelPortal:   A3N1S8
- STRING:   A3N1S8
- GeneID:   4848849
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1278
- eggNOG:   COG1663
- HOGENOM:   HBG663914
- OMA:   VILMDDG
- PhylomeDB:   A3N1S8
- ProtClustDB:   PRK00652
- BioCyc:   APLE416269:APL_1278-MONOMER
- HAMAP:   MF_00409
- InterPro:   IPR003758
- TIGRFAMs:   TIGR00682

Pfam domain/function: PF02606 LpxK

EC number: =2.7.1.130

Molecular weight: Translated: 36237; Mature: 36237

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIWQSTSIITWLLAPFSLLFWLVSQIRLFLFRKKILKSYRSPVPVLVVGNISVGGNGKT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCC
PVVVWLVEQLQQRGVKVGVISRGYGGKSKDFPQLVTNQSSAEMVGDEPVLIVQRTGVPLA
CEEEHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHCCCCCEEEEEECCCCEE
ISANRQQSIELLLNQFKLDLIVTDDGLQHYALQRDIEWVVVDGIRRFGNGFVLPAGGLRE
EECCCHHHHHHHHHHHEEEEEEECCCHHHHHHHCCCCEEEEHHHHHHCCCEEECCCCHHH
LPSRLQSVQAIICNGGKAQPNEHLMTLEPEFAVNLRTGEQKPITDFIGQECVAIAGIGHP
HHHHHHHHHEEEECCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHCCHHEEEECCCCC
PRFFNMLENLGVKLLKTQGFADHQAFEPAQLKALAAEQIPLLMTEKDAVKCRTFAQQNWW
HHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCE
YVPVSAKFSPESTACLLEPILKRLGK
EEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIWQSTSIITWLLAPFSLLFWLVSQIRLFLFRKKILKSYRSPVPVLVVGNISVGGNGKT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCC
PVVVWLVEQLQQRGVKVGVISRGYGGKSKDFPQLVTNQSSAEMVGDEPVLIVQRTGVPLA
CEEEHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHCCCCCEEEEEECCCCEE
ISANRQQSIELLLNQFKLDLIVTDDGLQHYALQRDIEWVVVDGIRRFGNGFVLPAGGLRE
EECCCHHHHHHHHHHHEEEEEEECCCHHHHHHHCCCCEEEEHHHHHHCCCEEECCCCHHH
LPSRLQSVQAIICNGGKAQPNEHLMTLEPEFAVNLRTGEQKPITDFIGQECVAIAGIGHP
HHHHHHHHHEEEECCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHCCHHEEEECCCCC
PRFFNMLENLGVKLLKTQGFADHQAFEPAQLKALAAEQIPLLMTEKDAVKCRTFAQQNWW
HHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCE
YVPVSAKFSPESTACLLEPILKRLGK
EEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA