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The map label for this gene is gltX

Identifier: 126208741

GI number: 126208741

Start: 1466737

End: 1468176

Strand: Direct

Name: gltX

Synonym: APL_1275

Alternate gene names: 126208741

Gene position: 1466737-1468176 (Clockwise)

Preceding gene: 126208737

Following gene: 126208742

Centisome position: 64.49

GC content: 43.82

Gene sequence:

>1440_bases
ATGAATATCGAAACTCTTTTCCCTTTAGATCCGAATGTAAAAGTGCGTACCCGTTTTGCACCTAGCCCGACCGGCTATTT
ACACGTTGGCGGCGCACGTACCGCTTTATCTTCTTGGCTATATGCAAAACACTTTAACGGTGAATTTGTATTACGTATCG
AAGATACCGACTTAGAACGCTCAACACCGGAAGCAACCGCTGCAATTCTTGAAGGTATGGAATGGTTAAATCTTGCTTGG
GAACACGGCCCATACTATCAAACCAAACGTTTTGACCGTTATAACCAAGTTATCGATCAAATGATCGAACAAGGTTTAGC
ATACCGTTGCTACTGCTCAAAAGAACGTTTGGAAAACTTACGTCACGAACAAGAAGCGAATAAAGAAAAACCTCGCTACG
ACCGTCATTGTTTAGCTCATCACGATCAACCTACGGATGCGCCGCACGTTGTGCGTTTTAAAAATCCGCAAGAAGGTTCG
GTAGTGTTTGACGATGCGGTACGCGGTCGTATCGAAATCAGTAATAGCGAATTAGACGATTTAATTATTCGCCGTACGGA
CGGTTCGCCGACTTATAACTTCTGTGTGGTGGTAGATGACTGGGATATGGGGATTACCCACGTTGTTCGCGGTGAAGATC
ATATCAACAATACGCCTCGTCAAATCAATATCTTAAAAGCGTTAGGCGCACCGATTCCGACTTACGCTCACGTGTCAATG
ATTAACGGTGATGACGGTCAAAAATTATCGAAACGCCACGGGGCGGTAAGCGTAATGCAATATCGTGACGACGGTTATTT
ACCGGAAGCGTTAATTAACTATTTAGTCCGTTTAGGTTGGGGGCACGGCGACCAAGAAATCTTTAGCCGTGAAGAAATGA
TCGAATTATTCGACATTCACTCGGTAAGCAAATCGGCAAGTGCGTTCAATACCGATAAATTACAATGGTTAAACCAACAC
TATATGCGTAGTTTACCAGCCGAACACGTGGCAAAATATTTAGCGTGGCATATGAATGATCAAGCTATCGATACTTCAAG
CGGCCCTGCATTAGAAGAAATCATTCCGGTATTAAGCGAACGTGCGAAAACATTAAAAGAATTAGCTGCCGCGAGCCGTT
ATTTCTATCAAGAATTTGATGGATATGATGAAAAAGCTGCGGCGAAAAACTTCAAAGCGGAAGCGGTTGCACCACTTGCA
AAATTATTGGAAAAATTAACCGCTTTAACCGATTGGAGCGTAGAAGCGATTCACGATGCGATGAATGCGACTGCGGCTGA
TTTAGAAATCGGTATGGGTAAAGTAGGGATGCCGTTCCGTTTAGCGGTAACCGGTTCCGGTCAATCGCCGTCAATGGATA
TTACTGCAAAATTAGTCGGACGTGAACGTACTTTAGCTCGTATCCAAAAAGCGATTGAGTTTATTCAAGCGCAAGCATAA

Upstream 100 bases:

>100_bases
CGTTTTTTAACCTTCAACCACCGGATTAACCGTAGTAGAATAGAAAAATCTTTTGTTTAAAAAAGCCAATCACGGCAAAT
CATTTAATAGGCTGATAATA

Downstream 100 bases:

>100_bases
TTTCCTATTGACAGTTACAGTGGCTACATTTATTATGTCGCCACTTTTCGGGGATATAGCTCAGTTGGGAGAGCGCTTGA
ATGGCATTCAAGAGGTCGTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALSSWLYAKHFNGEFVLRIEDTDLERSTPEATAAILEGMEWLNLAW
EHGPYYQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLENLRHEQEANKEKPRYDRHCLAHHDQPTDAPHVVRFKNPQEGS
VVFDDAVRGRIEISNSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMIELFDIHSVSKSASAFNTDKLQWLNQH
YMRSLPAEHVAKYLAWHMNDQAIDTSSGPALEEIIPVLSERAKTLKELAAASRYFYQEFDGYDEKAAAKNFKAEAVAPLA
KLLEKLTALTDWSVEAIHDAMNATAADLEIGMGKVGMPFRLAVTGSGQSPSMDITAKLVGRERTLARIQKAIEFIQAQA

Sequences:

>Translated_479_residues
MNIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALSSWLYAKHFNGEFVLRIEDTDLERSTPEATAAILEGMEWLNLAW
EHGPYYQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLENLRHEQEANKEKPRYDRHCLAHHDQPTDAPHVVRFKNPQEGS
VVFDDAVRGRIEISNSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMIELFDIHSVSKSASAFNTDKLQWLNQH
YMRSLPAEHVAKYLAWHMNDQAIDTSSGPALEEIIPVLSERAKTLKELAAASRYFYQEFDGYDEKAAAKNFKAEAVAPLA
KLLEKLTALTDWSVEAIHDAMNATAADLEIGMGKVGMPFRLAVTGSGQSPSMDITAKLVGRERTLARIQKAIEFIQAQA
>Mature_479_residues
MNIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALSSWLYAKHFNGEFVLRIEDTDLERSTPEATAAILEGMEWLNLAW
EHGPYYQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLENLRHEQEANKEKPRYDRHCLAHHDQPTDAPHVVRFKNPQEGS
VVFDDAVRGRIEISNSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMIELFDIHSVSKSASAFNTDKLQWLNQH
YMRSLPAEHVAKYLAWHMNDQAIDTSSGPALEEIIPVLSERAKTLKELAAASRYFYQEFDGYDEKAAAKNFKAEAVAPLA
KLLEKLTALTDWSVEAIHDAMNATAADLEIGMGKVGMPFRLAVTGSGQSPSMDITAKLVGRERTLARIQKAIEFIQAQA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=495, Percent_Identity=30.9090909090909, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI62241042, Length=269, Percent_Identity=25.6505576208178, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=69.6120689655172, Blast_Score=699, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=36.7768595041322, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1786895, Length=122, Percent_Identity=31.1475409836066, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=481, Percent_Identity=30.1455301455301, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17511007, Length=250, Percent_Identity=24, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=31.936127744511, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324742, Length=210, Percent_Identity=30, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321192, Length=241, Percent_Identity=28.6307053941909, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=32.1063394683027, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI17864488, Length=121, Percent_Identity=33.0578512396694, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_ACTP2 (A3N1S5)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053966.1
- ProteinModelPortal:   A3N1S5
- SMR:   A3N1S5
- STRING:   A3N1S5
- GeneID:   4849358
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1275
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   A3N1S5
- ProtClustDB:   PRK01406
- BioCyc:   APLE416269:APL_1275-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54191; Mature: 54191

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 251-251

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALSSWLYAKHFNGEFVLRIEDTDLER
CCCCEECCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCC
STPEATAAILEGMEWLNLAWEHGPYYQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLENL
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
RHEQEANKEKPRYDRHCLAHHDQPTDAPHVVRFKNPQEGSVVFDDAVRGRIEISNSELDD
HHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCCEEEECCCCCCC
LIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
EEEEECCCCCCEEEEEEEECCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCEEEEEE
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMIELFDIH
EECCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SVSKSASAFNTDKLQWLNQHYMRSLPAEHVAKYLAWHMNDQAIDTSSGPALEEIIPVLSE
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
RAKTLKELAAASRYFYQEFDGYDEKAAAKNFKAEAVAPLAKLLEKLTALTDWSVEAIHDA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MNATAADLEIGMGKVGMPFRLAVTGSGQSPSMDITAKLVGRERTLARIQKAIEFIQAQA
HCCCHHHHEECCCCCCCCEEEEEECCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALSSWLYAKHFNGEFVLRIEDTDLER
CCCCEECCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCC
STPEATAAILEGMEWLNLAWEHGPYYQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLENL
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
RHEQEANKEKPRYDRHCLAHHDQPTDAPHVVRFKNPQEGSVVFDDAVRGRIEISNSELDD
HHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCCEEEECCCCCCC
LIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
EEEEECCCCCCEEEEEEEECCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCEEEEEE
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMIELFDIH
EECCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SVSKSASAFNTDKLQWLNQHYMRSLPAEHVAKYLAWHMNDQAIDTSSGPALEEIIPVLSE
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
RAKTLKELAAASRYFYQEFDGYDEKAAAKNFKAEAVAPLAKLLEKLTALTDWSVEAIHDA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MNATAADLEIGMGKVGMPFRLAVTGSGQSPSMDITAKLVGRERTLARIQKAIEFIQAQA
HCCCHHHHEECCCCCCCCEEEEEECCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA